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Citations for Promega :
1201 - 1236 of 1236 citations for RT qPCR Master Mixes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Insertion sites in individual clones were mapped using a two-step nested arbitrary PCR approach using 2X GoTaq master mix (Promega M7122). The first reaction (1X GoTaq master mix ...
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bioRxiv - Microbiology 2023Quote: ... Marker genes in isolated DNA were polymerase chain reaction (PCR)-amplified using GoTaq Master Mix (Cat. No. M5133, Promega, Madison, Wisconsin) and 515 F (5′-GTGCCAGCMGCCGCGGTAA-3′) ...
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bioRxiv - Molecular Biology 2023Quote: ... The PEG10-5’AmC6-modified primers were used for the amplification of the donor template using GoTaq® Green Master Mix DNA polymerase (Promega). The SmUbi-EGFP-SmUbi construct with the 600 bp HA sequence (21 ...
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bioRxiv - Biochemistry 2023Quote: ... and the DNA fragments encoding the designs were amplified following a colonyPCR protocol using GoTaq® Green DNA polymerase master mix (#M7122; Promega) and T7 reverse and forward primers ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and a final extension at 72 °C for 10 min using GoTaq® G2 Hot Start Green Master Mix (Promega, USA). The PCR products of each gene were then separated on 2% agarose gels containing SYBR® Safe DNA Gel Stain (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: PCRs for amplification of chromosomal termini were performed on tailed DNA in 20 µL using GoTaq® Long PCR master Mix (Promega). PCR mix included 10 µL of GoTaq® Long PCR master Mix ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized using 5 μl of the eluent as template using the U3 antisense primer 5’ TGTGTGGTAGATCCACATCG-3’ and M-MLV RT RNase [H-] (Promega, WI. Cat. No. MR3681) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... according to manufacturer’s instructions and analyzed for the presence of the wildtype and knock-in Lmna allele by PCR using the GoTaq green master mix (Promega, Madison, WI) and the following primer pairs (see also ‘Primer and sgRNA sequences’):
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bioRxiv - Molecular Biology 2019Quote: ... PCR was performed using primers spanning 75-150 base pairs (Table S5) for each transcript using GoTaq® Green Master Mix (Promega, M7122) for 35 cycles.
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bioRxiv - Bioengineering 2021Quote: ... Presence of endogenous and/or exogenous Nix in the genome was assessed by PCR using GoTaq® Green Master Mix (M712, Promega, France) according to the manufacturer’s recommendations with the Nix-833 published primers 25 and primer EM1926-EM1927 (Supplementary Table 1) ...
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bioRxiv - Molecular Biology 2022Quote: ... and a 20 μl reaction volume was prepared for each reaction using the GoTaq® Green Master 2X Mix (M7122, Promega, Belgium) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Synthesized cDNA as described above was used to measure levels of unspliced and spliced XBP1mRNA were measured by regular PCR performed with GoTaq Green Master Mix (Promega cat#: M7123) on a T100 Thermo Cycler (Bio-Rad cat# ...
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bioRxiv - Physiology 2019Quote: An aliquot of cDNA obtained by reverse transcription was amplified in a PCR reaction with GoTaq Green Master Mix (Promega, Milan, Italy). For each pair of primers ...
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bioRxiv - Microbiology 2023Quote: ... 5’-CGCCTTCGTTACAAGCATCG-3’; antisense, 5’-AAGAGCAAACGCATCTCCGA-3’), GAPDH (sense, 5’-ACCCACTCCTCCACCTTTGAC-3’; antisense, 5’-CTGTTGCTGTAGCCAAATTCGT-3’) using Green GoTaq master mix (Promega, Madison, WI) with C1000 Touch Thermal Cycler (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2023Quote: ... one microliter volumes of Reverse Transcription reactions were used as template for amplification of internal cDNA fragments using in 20 μl volume PCR reactions (35 rounds of amplification using Promega 2X PCR Master Mix (Promega, Madison, WI). Gene-specific primers and annealing temperatures are listed in Table 1 ...
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bioRxiv - Molecular Biology 2023Quote: Endogenous target sites and reporter target sites for arrayed editing were amplified by GoTaq G2 Hot Start Green Master Mix (Promega, 30 cycles) and were purified with Sera-Mag Select (Merck) ...
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bioRxiv - Microbiology 2024Quote: ... we confirmed that each primer set amplified single bands in a ligation-dependent manner by GoTaq Hot Start Green Master Mix (Promega, Madison, WI) (Figure S10) ...
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bioRxiv - Molecular Biology 2020Quote: ... purified amplicons from each single-plex TaqMan® qPCR were cloned into a plasmid vector using the pGEM®-T Easy Vector System I kit (Cat.# A1360, Promega, Walldorf, Germany) and One Shot® TOP10 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... The fragment sizes provided by the Fragment Analyzer were compared to the degradation indices obtained from real-time quantitative polymerase chain reaction (qPCR) with PowerQuant System (Promega Corporation, Madison, WI, USA). After one year of storage in − 20 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... 30 min at 60°C) an alkylation (IAA 0.5 M, 30 min RT) proteins were digested using trypsin (Gold, Promega, 1ug / sample, overnight at 37°C). For LC MSMS analysis ...
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bioRxiv - Cell Biology 2020Quote: ... Two microliters aliquots of the generated cDNA was amplified in 50 μl of PCR reaction mixture containing 2X GoTaq® green master mix (Promega, Madison, WI), 100 nM of each primer and ddH2O ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCRs were performed in a total volume of 10µl (1µl DNA sample, 3µl Milli-Q water, 5µl GOTag Green Master Mix buffer (Promega, note with 1.5mM MgCl2 for 1x reaction), 0.5µl forward primer and of reverse primer each) ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by 85℃ heat inactivation for 1 hour and PCR-based genotyping (GoTaq Green Master Mix, Promega, and C1000 Touch Cycler, Bio-rad). Tcf12 [34] ...
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bioRxiv - Cell Biology 2021Quote: ... 30 min at 60°C) an alkylation (IAA 0.5M, 30 min RT) microtubule-associated protein enriched fractions were digested using trypsin (Gold, Promega, 1 μg / sample, overnight at 30°C). Peptide clean-up was done using OMIX C18 (Agilent ...
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bioRxiv - Neuroscience 2021Quote: ... All animals were genotyped according to the MMRRC strain-specific primers and protocols using GoTaq Green PCR master mix (Cat. No. M712, Promega Corporation, Madison, WI, USA).
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bioRxiv - Neuroscience 2022Quote: ... All animals were genotyped according to the MMRRC strain-specific primers and protocols using GoTaq Green PCR master mix (Cat. No. M712, Promega Corporation, Madison, WI, USA).
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bioRxiv - Developmental Biology 2020Quote: ... Tail biopsies of mice were lysed by a HotShot protocol (incubating the tail sample at 95°C for 30 min in an alkaline lysis reagent followed by neutralization) and used for PCR-based genotyping (GoTaq Green Master Mix, Promega, and Nexus X2, Eppendorf). Perinatal mice were also genotyped fluorescently (BLS miner’s lamp ...
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Throat colour polymorphism in relation to sex and body size of the Litter skink, Lankascincus fallaxbioRxiv - Zoology 2022Quote: ... The PCR amplification was carried out in 25 μl volumes employing 35 cycles with an annealing temperature of 55 °C following standard PCR protocols with Promega PCR master mix (Promega Corporation, Madison, Wisconsin, USA). The success of the PCR amplification of each sample was confirmed through Gel electrophoresis using 5 μl of each sample ...
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bioRxiv - Microbiology 2024Quote: ... Amplifications were set up in a total volume of 20 μL containing: 10 μL GoTaq® Green Master Mix (Promega Corporation, Madison, Wisconsin, USA), 1 μL of each 10 μM primer ...
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bioRxiv - Developmental Biology 2023Quote: ... Tail biopsies of mice were lysed by a HotShot protocol (incubating the tail sample at 95°C for 30 min in an alkaline lysis reagent followed by neutralization) and used for PCR-based genotyping (GoTaq Green Master Mix, Promega, and Nexus X2, Eppendorf). Perinatal mice were also genotyped fluorescently (BLS miner’s lamp ...
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bioRxiv - Microbiology 2023Quote: ... using the described conditions for PCR in 25 µL using GoTaq® G2 DNA Polymerase and Master Mix (Promega Benelux BV, Leiden, the Netherlands), 10 µM of each primer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 5 µL of amplificon was amplified in a 25 µL mixture containing 12.5 µL of GoTaq® Green Master Mix 2X (Promega, Madison, Wisconsin, United States), 2.5 μL of each primer at 10 μM TGV (5’-TGTAACTCGGTGTAYGGNTTYACNGGNGT-3’ ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 2 µL of DNA was amplified in a 25 µL mixture containing 12.5 µL of GoTaq® Green Master Mix 2X (Promega, Madison, Wisconsin, United States), 2.5 μL of each primer at 10 μM DFA (5’-GAYTTYGCNAGYYTNTAYCC-3’ ...
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bioRxiv - Microbiology 2021Quote: ... and the near full-length 16S rRNA gene was amplified using primers 8F (5’-AGAGTTTGATCCTGGCTCAG-3’) and 1492R (5’-TACGGTTACCTTGTTACGA-3’) with the GoTaq® Hot Start Colorless Master Mix (Promega Corp., Madison, WI, USA). PCR was performed using Eppendorf Vapo Protect Mastercycler Pro S 6325 (Hamburg ...
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Exploring the interspecific interactions and the metabolome of the soil isolate Hylemonella gracilisbioRxiv - Microbiology 2022Quote: ... gracilis 16S rRNA PCR was performed from grown colonies in a 50 μl PCR-GoTaq™ green master mix (Promega Corp. Madison, USA cat# M712). For 16S rRNA gene amplification the following primers were used ...
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bioRxiv - Developmental Biology 2020Quote: ... Tail biopsies of mice were lysed by a HotShot protocol (incubating the tail sample at 95°C for 30 min in an alkaline lysis reagent followed by neutralization) and used for GoTaq Green Master Mix PCR-based genotyping (Promega, and Nexus X2, Madison, WI). Mice were euthanized by over-dosage of carbon dioxide or decapitation under inhalation anesthesia in a drop jar (Fluriso ...