Labshake search
Citations for Promega :
1151 - 1165 of 1165 citations for RT qPCR Master Mixes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 30 min at 60°C) an alkylation (IAA 0.5 M, 30 min RT) proteins were digested using trypsin (Gold, Promega, 1ug / sample, overnight at 37°C). For LC MSMS analysis ...
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bioRxiv - Cell Biology 2020Quote: ... Two microliters aliquots of the generated cDNA was amplified in 50 μl of PCR reaction mixture containing 2X GoTaq® green master mix (Promega, Madison, WI), 100 nM of each primer and ddH2O ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCRs were performed in a total volume of 10µl (1µl DNA sample, 3µl Milli-Q water, 5µl GOTag Green Master Mix buffer (Promega, note with 1.5mM MgCl2 for 1x reaction), 0.5µl forward primer and of reverse primer each) ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by 85℃ heat inactivation for 1 hour and PCR-based genotyping (GoTaq Green Master Mix, Promega, and C1000 Touch Cycler, Bio-rad). Tcf12 [34] ...
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bioRxiv - Cell Biology 2021Quote: ... 30 min at 60°C) an alkylation (IAA 0.5M, 30 min RT) microtubule-associated protein enriched fractions were digested using trypsin (Gold, Promega, 1 μg / sample, overnight at 30°C). Peptide clean-up was done using OMIX C18 (Agilent ...
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bioRxiv - Neuroscience 2021Quote: ... All animals were genotyped according to the MMRRC strain-specific primers and protocols using GoTaq Green PCR master mix (Cat. No. M712, Promega Corporation, Madison, WI, USA).
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bioRxiv - Neuroscience 2022Quote: ... All animals were genotyped according to the MMRRC strain-specific primers and protocols using GoTaq Green PCR master mix (Cat. No. M712, Promega Corporation, Madison, WI, USA).
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bioRxiv - Developmental Biology 2020Quote: ... Tail biopsies of mice were lysed by a HotShot protocol (incubating the tail sample at 95°C for 30 min in an alkaline lysis reagent followed by neutralization) and used for PCR-based genotyping (GoTaq Green Master Mix, Promega, and Nexus X2, Eppendorf). Perinatal mice were also genotyped fluorescently (BLS miner’s lamp ...
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Throat colour polymorphism in relation to sex and body size of the Litter skink, Lankascincus fallaxbioRxiv - Zoology 2022Quote: ... The PCR amplification was carried out in 25 μl volumes employing 35 cycles with an annealing temperature of 55 °C following standard PCR protocols with Promega PCR master mix (Promega Corporation, Madison, Wisconsin, USA). The success of the PCR amplification of each sample was confirmed through Gel electrophoresis using 5 μl of each sample ...
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bioRxiv - Developmental Biology 2023Quote: ... Tail biopsies of mice were lysed by a HotShot protocol (incubating the tail sample at 95°C for 30 min in an alkaline lysis reagent followed by neutralization) and used for PCR-based genotyping (GoTaq Green Master Mix, Promega, and Nexus X2, Eppendorf). Perinatal mice were also genotyped fluorescently (BLS miner’s lamp ...
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bioRxiv - Microbiology 2023Quote: ... using the described conditions for PCR in 25 µL using GoTaq® G2 DNA Polymerase and Master Mix (Promega Benelux BV, Leiden, the Netherlands), 10 µM of each primer ...
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bioRxiv - Microbiology 2024Quote: ... Amplifications were set up in a total volume of 20 μL containing: 10 μL GoTaq® Green Master Mix (Promega Corporation, Madison, Wisconsin, USA), 1 μL of each 10 μM primer ...
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bioRxiv - Microbiology 2021Quote: ... and the near full-length 16S rRNA gene was amplified using primers 8F (5’-AGAGTTTGATCCTGGCTCAG-3’) and 1492R (5’-TACGGTTACCTTGTTACGA-3’) with the GoTaq® Hot Start Colorless Master Mix (Promega Corp., Madison, WI, USA). PCR was performed using Eppendorf Vapo Protect Mastercycler Pro S 6325 (Hamburg ...
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Exploring the interspecific interactions and the metabolome of the soil isolate Hylemonella gracilisbioRxiv - Microbiology 2022Quote: ... gracilis 16S rRNA PCR was performed from grown colonies in a 50 μl PCR-GoTaq™ green master mix (Promega Corp. Madison, USA cat# M712). For 16S rRNA gene amplification the following primers were used ...
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bioRxiv - Developmental Biology 2020Quote: ... Tail biopsies of mice were lysed by a HotShot protocol (incubating the tail sample at 95°C for 30 min in an alkaline lysis reagent followed by neutralization) and used for GoTaq Green Master Mix PCR-based genotyping (Promega, and Nexus X2, Madison, WI). Mice were euthanized by over-dosage of carbon dioxide or decapitation under inhalation anesthesia in a drop jar (Fluriso ...