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Citations for Promega :
1051 - 1100 of 1165 citations for RT qPCR Master Mixes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Marker genes in isolated DNA were PCR-amplified using GoTaq Master Mix (Cat. No. M5133, Promega, Madison, WI, USA) as described in Caporaso et al.[24] PCR products were cleaned and normalized using the SequalPrepTM Normalization Plate Kit (Cat ...
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bioRxiv - Synthetic Biology 2022Quote: ... The presence and orientation of DNA fragments were confirmed by colony PCR using GoTaq Green Master Mix (Promega, USA), whereas the sequence of the genetic construct was checked by Sanger sequencing reactions (Macrogen Inc. ...
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bioRxiv - Molecular Biology 2022Quote: Edited loci were amplified using locus-specific primer pairs described in Supplementary Table 1 using GoTaq master mix (Promega) and 200ng of genomic DNA ...
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bioRxiv - Plant Biology 2023Quote: ... Gene-specific and transposon multiplex primer PCR was performed under standard conditions with 2X GoTaq Green Master Mix (Promega) with 5% DMSO (v/v).
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bioRxiv - Neuroscience 2023Quote: Adult worms were lysed following standard protocols and PCRs were performed using GoTaq Green Master Mix (Promega, REF-M7123). Mutant alleles were distinguished from wild-type by imaging Restriction Fragment Length Polymorphisms (RFLP ...
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bioRxiv - Plant Biology 2023Quote: ... gRNA-targeted regions were amplified from genomic DNA isolated from the T1 plants with GoTaq Green Master Mix (Promega) using the primers g1-seqF/g1-seqR and g2-seqF/g2-seqR for gRNA1 and gRNA2 ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μg of pCMV-Sport- Polκ were incubated with 100 µL of TnT® Sp6 Quick Master Mix (Promega) supplemented with 4 μL of 1 mM methionine for 90 min at 30°C ...
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bioRxiv - Neuroscience 2023Quote: ... For all animals universal PCR reaction was used as follows: 12.5 μl of GoTaq Hot Start Green Master Mix (Promega), 0.5 μl of each primer (25 μM ...
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bioRxiv - Plant Biology 2022Quote: ... 6.5 μl of 1:100 diluted cDNA per reaction were used with the GoTaq® qPCR Mastermix (Promega GmbH, Madison, USA) in a total volume of 15 μl ...
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bioRxiv - Cancer Biology 2023Quote: ... washed with PBS and inflammasome activation was assessed using qPCR or inflammasome caspase-1 activity was measured using Caspase-Glo® 1 inflammasome assay kit (G9951; Promega) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... and gene expression was determined by quantitative PCR (QuantStudio 6 Pro Real-Time PCR System) with GoTag qPCR System (Promega, A6002). Actb and Rplp0 were used as housekeeping genes ...
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bioRxiv - Plant Biology 2020Quote: ... corniculatus plants was synthesized from 3 μg of total RNA using a Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) (Promega, WI, USA) and 100 pmol of random hexamers (Pharmacia Biotech) ...
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bioRxiv - Developmental Biology 2020Quote: The coding region of her9 was amplified by RT-PCR and the cDNA was cloned into a pGEMT-easy plasmid (Promega, Madison, WI). Capped mRNA was synthesized using the mMessage T7 kit (Ambion ...
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bioRxiv - Biochemistry 2020Quote: ... Moloney murine leukemia virus reverse transcriptase (MMLV-RT) enzyme and recombinant RNasin® ribonuclease inhibitor were purchased from Promega (Madison, WI, USA). Evagreen 2X qPCR MasterMix was purchased from Applied Biological Materials Inc ...
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bioRxiv - Immunology 2021Quote: ... Luminescence was read after 2 minutes shaking on an orbital shaker and 10 min incubation at RT using the GloMax instrument (Promega, Madison, USA).
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bioRxiv - Molecular Biology 2020Quote: ... all RT mixture of samples were pooled and purified with Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA) according to the manufacture’s manual except for using 80% EtOH instead of Membrane Wash Solution ...
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bioRxiv - Plant Biology 2022Quote: ... cDNAs were synthesized from 1.5 μg of total RNA with oligo(dT) primers using Moloney Murine Leukemia Virus Reverse Transcriptase MMLV-RT according to the manufacturer’s instructions (Promega, Madison, WI, USA). RT-qPCR was carried out in a Chromo4 system (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... the buffer was diluted with 50 mM ABC to a final of 2M urea and digested overnight at RT with 1 µg trypsin (Promega, Walldorf, Germany). On the next day ...
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bioRxiv - Microbiology 2024Quote: ... followed by reverse transcription to complementary DNA (cDNA) using 30 enzyme units(U) of the Moloney Murine Leukemia Virus reverse transcriptase (MMLV-RT) (Promega, WI, USA). The extension products were then analysed using the ABI3730xl DNA Analyzer (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... PCRs were used to assay for wild-type or T-DNA insertion alleles in 20 μL reactions using GoTaq Green Master Mix (Promega) using manufacturer’s instructions and 30 cycles as follows ...
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bioRxiv - Genetics 2021Quote: ... a 599 bp long construct was PCR amplified from genomic DNA isolated from the tail of a Pahenu2 homozygous animal using GoTaq G2 HotStart Green Master Mix (Promega). Primers are listed in Suppl ...
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bioRxiv - Developmental Biology 2021Quote: ... Real time PCR was performed on an ABI Prism 7900 detection system using Gotaq master mix (Promega, Madison, WI, USA). Expression relative to the housekeeping gene TBP was calculated using the delta(d)Ct method and expressed as 2^(-dCT ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Cytochrome B was amplified from the same saliva sample in which DrDV-B was detected using primers Bat 05A and Bat 04A (45) and GoTaq Green Master Mix (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The amplification was performed in 25 μl mixtures containing 12.5 μl GoTaq□Green Master Mix (Promega, Madison, Wl, United States), 0.625 μl of each primer (20mM) ...
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bioRxiv - Microbiology 2022Quote: ... The amplification was performed in 50 μl mixtures containing 20 μl GoTaq□Green Master Mix (Promega, Madison, Wl, United States), 1 μl of each primer (20 mM) ...
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bioRxiv - Molecular Biology 2021Quote: A conventional PCR targeting the hsp65 gene was conducted using the GoTaq® Green Master Mix (Promega, Madison, Wisconsin, USA) in a final reaction volume of 13μl comprising 6.25μl of 2X GoTaq Hot Start Green Master Mix ...
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bioRxiv - Molecular Biology 2019Quote: ... qPCR amplifications (12 μl) were done in duplicates using 1 μl of cDNA and the GoTaq Probe 2X Master Mix (Promega) on a LightCycler 480 (Roche) ...
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bioRxiv - Bioengineering 2020Quote: ... The second round of PCR was run with 10μL GoTaq Green Master Mix (Promega Biosciences LLC, San Luis Obispo, CA), 4.2μL of water ...
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bioRxiv - Neuroscience 2021Quote: Genomic DNA was extracted using HotSHOT (hot sodium hydroxide and tris) and PCR was performed using GoTaq green master mix (Promega) (Truett et al ...
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bioRxiv - Microbiology 2020Quote: ... Twenty nanograms of DNA were used as a model for 18 cycles of amplification of the V4 region of the 16S rRNA gene, using primers 515F and 806R (Caporaso, et al. 2012: 1621-4) and GoTaq Master Mix (Promega). PCR products were quantified with the Qschd dsDNA HS kit (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR was performed with 40 ng of cDNA for the cell and 1.6 ng of cDNA as the template and GoTaq™ master mix according to manufacturer instructions (Promega). The oligonucleotides were designed with PRIMER-Blast using the following parameters ...
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bioRxiv - Bioengineering 2020Quote: ... The second round of PCR was run with 10μL GoTAQ Green Master Mix (Promega Biosciences LLC, San Luis Obispo, CA), 4.2μL of water ...
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bioRxiv - Cancer Biology 2022Quote: ... a gene-specific amplicon was generated in a 20 μL reaction for 35 cycles with GoTaq G2 Green Master Mix (Promega). The PCR product was purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) ...
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bioRxiv - Plant Biology 2019Quote: ... subspecies nebraskensis were carried out in 20 μl reactions containing 10 μl of 2X GoTaq® Green Master Mix (Promega), 1 μl of each forward and reverse primer (5 μM) ...
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bioRxiv - Genetics 2020Quote: PCR used to detect the viral cDNA was performed using the GoTaq® Green Master Mix (Promega, Madison, WI, USA) under the following conditions ...
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bioRxiv - Molecular Biology 2021Quote: ... PAT-PCR was performed using a 3’ UTR-specific forward primer and a y300 PAT universal C10 primer with GoTaq Green Master Mix (Promega). The primer sequences are provided in Table S1 ...
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bioRxiv - Microbiology 2021Quote: 16S rRNA gene of each isolate was amplified from its genomic DNA using GoTaq G2 Hot Start Master Mix (Promega) and the purified PCR products were sequenced using BigDye Terminator v3.1 Cycle Sequencing Kit according to a previous report [15].
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR was performed in a 25 µl reaction volume with 12.5 µl of GoTaq ® Green Master Mix (Promega, USA), 1 µl of 100 ng/µl gDNA ...
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bioRxiv - Zoology 2021Quote: ... Polymerase chain reactions in a 15 μL solution containing 7.5 μL GoTaq® G2 Hot Start Green Master Mix (Promega), 0.4 μmol of each primer and 46 – 247 ng of genomic DNA were performed using a C1000 Touch Thermal Cylinder (BioRad) ...
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bioRxiv - Plant Biology 2021Quote: ... The DNA pellet was resuspended in water with RNase A (10 µg/mL) for amplicon library preparation using GoTaq G2 Green Master Mix (Promega) and primers (Supplemental Table S1 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... were then used to amplify a 443 bp region of the mitochondrial cox1 gene using GoTaq Hot Start Master Mix (Promega). Each 50 µL reaction contained 500 nM each primer and cycling conditions were as described above ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Each reaction was carried out in 15 μl volume containing 7.5 μl of GoTaq® Hot Start Green Master Mix (Promega), 0.5 μl each of the forward and reverse primer ...
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bioRxiv - Genetics 2020Quote: ... 1-2 μL of cDNA was used for 25 μL PCR reactions using the GoTaq Hot Start Master Mix (Promega). Cycling parameters consisted of an initial denaturation of 95°C for 2 min. ...
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bioRxiv - Cancer Biology 2022Quote: ... Pups were screened for the deletion by classical genotyping PCR with the GoTaq R2 Hot Start Green Master Mix (Promega) with fw (5’-CCTCGGAAGCTGCCTAAGAT-3’) ...
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bioRxiv - Microbiology 2023Quote: ... The PCR reaction was performed in 25 μL final volumes and consisted of 12.5 μL GoTaq® G2 Hot Start Colorless Master Mix (2X - Promega), 1 μL of each primer (10 μM) ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR Tag analysis was performed either by adding 1.8 μL of yeast lysate used as template DNA to 6.25 μL of 2X GoTaq® Green Master Mix (Promega), 4.75 μL of nuclease-free water and 400 nM of each primer or by adding 1 µl of yeast lysate to 5 µl of DreamTaq Green PCR Master Mix (2X ...
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bioRxiv - Neuroscience 2023Quote: ... We performed targeted PCR by adding 1 μL of cell lysate (1:5 dilution) to a 25-μL PCR reaction containing GoTaq Hot Start Master Mix Green (Promega) and 0.5 μL of the primers (10 µM ...
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bioRxiv - Plant Biology 2023Quote: ... Approximately 20 ng of trichome DNA was amplified by combining 0.25 μM primer and 1X GoTaq Green Master Mix (Promega, USA) in a 50μl reaction volume ...
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bioRxiv - Bioengineering 2023Quote: ... Presumptive screening of 10 colonies per mPilA variant was performed via colony-PCR using GoTaq® Green Master Mix (Promega) and forward and reverse primers Diag:71301:MPilA:All:FWD and Diag:71301:MPilA:All:REV (SI Table S1) ...
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bioRxiv - Cell Biology 2023Quote: ... Presence of rat cells in myoblast culture/muscle lysate was assessed by PCR for rat dystrophin using primers Rat Dmd i22-i23.F/ Rat Dmd i22-i23.R and GoTaq G2 Hot Start Green Master Mix (M7423, Promega) via the following program ...