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Citations for Promega :
1101 - 1150 of 1165 citations for RT qPCR Master Mixes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... PCR reactions were conducted in 10 μL volume comprising 5 μL Promega 2x PCR Master Mix (Promega, Madison, Wisconsin, USA), 10 pmol of forward and reverse primers ...
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bioRxiv - Molecular Biology 2024Quote: ... PAT-PCR was performed using a 3′ UTR-specific forward primer and a PAT universal primer with GoTaq Green Master Mix (Promega). The PCR products were separated by 6% PAGE in 0.5× TBE ...
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bioRxiv - Molecular Biology 2023Quote: ... and target sites from the TRIP library were amplified by GoTaq G2 Hot Start Green Master Mix (Promega, 26 cycles). Amplicons were purified via gel extraction using a NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel) ...
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bioRxiv - Microbiology 2024Quote: ... Kanamycin-sensitive exconjugants were screened for the mutant with colony PCR using altgenoF and altgenoR primers designed outside of the deletion flanks with the following PCR reaction mix: 10 µl of GoTaq Green master mix (Promega), 0.5 µl of each primer at 10 µM concentration ...
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bioRxiv - Microbiology 2024Quote: ... 2 µL of extracted DNA was added to an amplification mixture containing 1 µl (10 µM) of primers and 12.5 μl of PCR Master Mix Plus (Promega, USA). To each mixture ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was extracted from lymphoma derived cell lines as described above and PCR amplified using sgRNA specific primers (Supplementary Table 3, p53 primers as previously described (13) and GoTaq Green Master Mix (Promega). PCR products were amplified a second time using indexing primers ...
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bioRxiv - Cancer Biology 2024Quote: ... Integrated sgRNAs in each lymphoma sample were amplified from 100 ng of genomic DNA using GoTaq Green Master Mix (Promega) and indexing primers with unique overhangs (12) ...
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bioRxiv - Developmental Biology 2024Quote: ... Digoxigenin-labelled or Fluorescein-labelled mRNA probes were either prepared as PCR probes with primers listed in Table S4 using the Kit GoTaq green master mix (Promega) or as described (Orgeur et al. ...
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Evolution of sex hormone binding globulins reveals early gene duplication at the root of vertebratesbioRxiv - Evolutionary Biology 2020Quote: ... Reverse transcription (RT) was performed using 1 μg of RNA for each sample with M-MLV reverse transcriptase and random hexamers (Promega, Madison, WI, USA). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... plates were removed from the incubator after 70 hours and 10 µL of 4x RT-Glo substrate and enzyme per well (Promega, Madison, WI, USA) diluted in P/S free medium ...
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bioRxiv - Microbiology 2020Quote: ... followed by a reverse transcription using a universal degenerated oligo specific to all 5′ non-coding sequences of BTV-1 segments (BTV/Uni1; 5′ GTTAAAWHDB 3′) and the GoScript Reverse Transcription (RT) System (Promega, Madison, WI, USA). The cDNAs obtained for the segment 7 were then quantified with a real time quantitative PCR (qPCR) ...
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bioRxiv - Pathology 2020Quote: ... cDNA synthesis from the mRNA was performed using a Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) kit (Promega, Madison, Wisconsin, USA) in a Mastercycler® Gradient (Eppendorf ...
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bioRxiv - Plant Biology 2022Quote: ... benthamiana leaf discs were ground to a fine powder in liquid nitrogen in 2 ml Eppendorf Safe-Lock tube (1 leaf disc in each tube) and subsequently incubated 5 min at room temperature (RT) with 200 µl of the Passive lysis buffer (Promega catalog number E1910). The resulting crude leaf extract was centrifuged at 20,000 g for 2 min at RT ...
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bioRxiv - Biochemistry 2024Quote: ... was synthesised from 2 µg of total RNA using the Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT; Promega, Madison, WI, USA) using random primers and oligo-dT primers (3:1 mol ...
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bioRxiv - Cancer Biology 2019Quote: Genomic DNA was isolated and region of sgPten targeting was PCR amplified using GoTaq G2 Green Master Mix (Promega, Cat. # M7823). The PCR product was purified using E.N.Z.A ...
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bioRxiv - Evolutionary Biology 2020Quote: ... All PCR reactions were performed at 25µl total with 12.5 ul 2X Promega Hot Start Master Mix (Promega Corporation, Madison, USA) and the primer conditions listed in Tab 1 ...
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bioRxiv - Plant Biology 2020Quote: ... Individual reactions conducted with UGA-CPES consisted of a 10 µL mixture containing 5 µL of GoTaq Green Master Mix 2X (Promega Corporation), 0.5 µL of template DNA ...
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bioRxiv - Cell Biology 2021Quote: ... Real time quantitative PCR was performed in a CFX 96 BioRad CFX 96 Real-Time PCR detection instrument with GoTaq qRT-PCR master mix (Promega, A6002). The sequence of the primers used in this study are ...
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bioRxiv - Molecular Biology 2022Quote: ... A 20 μl reaction volume was prepared for each reaction using the GoTaq® Green Master 2X Mix (M7122, Promega, Belgium) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... RT-PCR analysis of XBP1 splicing was carried out using: XBP1 F 5’-GGAGTTAAGACAGCGCTTGGGGA-3’ and XBP1 R 5’-TGTTCTGGAGGGGTGACAACTGGG-3’ oligonucleotides and GoTaq® Green Master Mix (Promega), using a 58⁰C annealing temperature for 25 cycles ...
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bioRxiv - Genomics 2021Quote: ... 23.5 μl PCR master mix (20 μl Buffer 5X, 2 μl dNTPs 10 μM, 1.5 μl GoTaq; Promega, cat. no. M3001), and 5 μl of Forward universal primer ...
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bioRxiv - Microbiology 2021Quote: ... The amplification was performed in 25 µl mixtures containing 12.5 µl GoTaq® Green Master Mix (Promega, Madison, WI, United States), 0.625 µl of each primer (20 µM) ...
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bioRxiv - Microbiology 2021Quote: ... The amplification was performed in 50 µl mixture containing 20 µl GoTaq®Green Master Mix (Promega, Madison, WI, United States), 1 µl of each primer (10 µM) ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was diluted 5 folds with distilled water and used in a reaction mix composed of 1x GoTaq qRT-PCR master mix (Promega, USA) and 10 nM primers for RT-qPCR ...
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bioRxiv - Genomics 2022Quote: ... The efficiency and specificity of the designed primers was tested through PCR using the GoTaq Green Master kit (Promega, Madison, USA) with thermal cycling conditions of 2 min at 95 °C ...
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bioRxiv - Synthetic Biology 2022Quote: ... and the integration of antibiotic resistance cassettes or reporter genetic constructs in the genome was confirmed by PCR under standard conditions using the GoTaq Green Master Mix (Promega, USA). The strains used and generated herein are shown in Table S1 ...
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bioRxiv - Microbiology 2023Quote: ... Each PCR reaction with a total volume of 25 μL contained 12.5 μL of PCR master mix (Promega; Madison, WI, USA), 8.0 μL of nuclease free water ...
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bioRxiv - Immunology 2023Quote: ... 25 ng of c-DNA was used per reaction in each well containing the 2X SYBR green PCR master mix (Promega, USA) along with appropriate primers ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR reactions were prepared in 25 μL final reaction volume containing 12,5 μL of GoTaq® G2 Hot Start Colorless Master Mix (Promega, USA), 0,25 μL (10 μM ...
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bioRxiv - Microbiology 2023Quote: Insertion sites in individual clones were mapped using a two-step nested arbitrary PCR approach using 2X GoTaq master mix (Promega M7122). The first reaction (1X GoTaq master mix ...
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bioRxiv - Microbiology 2023Quote: ... Marker genes in isolated DNA were polymerase chain reaction (PCR)-amplified using GoTaq Master Mix (Cat. No. M5133, Promega, Madison, Wisconsin) and 515 F (5′-GTGCCAGCMGCCGCGGTAA-3′) ...
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bioRxiv - Molecular Biology 2023Quote: ... The PEG10-5’AmC6-modified primers were used for the amplification of the donor template using GoTaq® Green Master Mix DNA polymerase (Promega). The SmUbi-EGFP-SmUbi construct with the 600 bp HA sequence (21 ...
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bioRxiv - Biochemistry 2023Quote: ... and the DNA fragments encoding the designs were amplified following a colonyPCR protocol using GoTaq® Green DNA polymerase master mix (#M7122; Promega) and T7 reverse and forward primers ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and a final extension at 72 °C for 10 min using GoTaq® G2 Hot Start Green Master Mix (Promega, USA). The PCR products of each gene were then separated on 2% agarose gels containing SYBR® Safe DNA Gel Stain (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... 25 ng of c-DNA was used per reaction in each well containing the 2X SYBR green PCR master mix (Promega, USA) along with appropriate primers ...
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bioRxiv - Neuroscience 2024Quote: ... One µL of lysate was then added to make 20µL PCR reactions with GoTaq G2 Green Master Mix (Promega, Cat# M7823) and 1µM forward and reverse primers (Table 1) ...
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bioRxiv - Genetics 2024Quote: ... 500–800 nt amplicons were amplified from genomic DNA from individual insertion lines through single fly PCR using GoTaq green master mix (Promega M7122).
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized using 5 μl of the eluent as template using the U3 antisense primer 5’ TGTGTGGTAGATCCACATCG-3’ and M-MLV RT RNase [H-] (Promega, WI. Cat. No. MR3681) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... according to manufacturer’s instructions and analyzed for the presence of the wildtype and knock-in Lmna allele by PCR using the GoTaq green master mix (Promega, Madison, WI) and the following primer pairs (see also ‘Primer and sgRNA sequences’):
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bioRxiv - Molecular Biology 2019Quote: ... PCR was performed using primers spanning 75-150 base pairs (Table S5) for each transcript using GoTaq® Green Master Mix (Promega, M7122) for 35 cycles.
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bioRxiv - Bioengineering 2021Quote: ... Presence of endogenous and/or exogenous Nix in the genome was assessed by PCR using GoTaq® Green Master Mix (M712, Promega, France) according to the manufacturer’s recommendations with the Nix-833 published primers 25 and primer EM1926-EM1927 (Supplementary Table 1) ...
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bioRxiv - Molecular Biology 2022Quote: ... and a 20 μl reaction volume was prepared for each reaction using the GoTaq® Green Master 2X Mix (M7122, Promega, Belgium) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Synthesized cDNA as described above was used to measure levels of unspliced and spliced XBP1mRNA were measured by regular PCR performed with GoTaq Green Master Mix (Promega cat#: M7123) on a T100 Thermo Cycler (Bio-Rad cat# ...
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bioRxiv - Physiology 2019Quote: An aliquot of cDNA obtained by reverse transcription was amplified in a PCR reaction with GoTaq Green Master Mix (Promega, Milan, Italy). For each pair of primers ...
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bioRxiv - Microbiology 2023Quote: ... 5’-CGCCTTCGTTACAAGCATCG-3’; antisense, 5’-AAGAGCAAACGCATCTCCGA-3’), GAPDH (sense, 5’-ACCCACTCCTCCACCTTTGAC-3’; antisense, 5’-CTGTTGCTGTAGCCAAATTCGT-3’) using Green GoTaq master mix (Promega, Madison, WI) with C1000 Touch Thermal Cycler (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2023Quote: ... one microliter volumes of Reverse Transcription reactions were used as template for amplification of internal cDNA fragments using in 20 μl volume PCR reactions (35 rounds of amplification using Promega 2X PCR Master Mix (Promega, Madison, WI). Gene-specific primers and annealing temperatures are listed in Table 1 ...
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bioRxiv - Molecular Biology 2023Quote: Endogenous target sites and reporter target sites for arrayed editing were amplified by GoTaq G2 Hot Start Green Master Mix (Promega, 30 cycles) and were purified with Sera-Mag Select (Merck) ...
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bioRxiv - Microbiology 2023Quote: ... we confirmed that each primer set amplified single bands in a ligation- dependent manner by GoTaq Hot Start Green Master Mix (Promega, Madison, WI) (Figure S6) ...
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bioRxiv - Molecular Biology 2020Quote: ... purified amplicons from each single-plex TaqMan® qPCR were cloned into a plasmid vector using the pGEM®-T Easy Vector System I kit (Cat.# A1360, Promega, Walldorf, Germany) and One Shot® TOP10 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... The fragment sizes provided by the Fragment Analyzer were compared to the degradation indices obtained from real-time quantitative polymerase chain reaction (qPCR) with PowerQuant System (Promega Corporation, Madison, WI, USA). After one year of storage in − 20 °C ...