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Citations for Promega :
1401 - 1450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and oligo(dT)15 primer (Promega). The mRNA transcript levels of selected IVSPER genes were measured by quantitative reverse transcription-PCR (qRT-PCR ...
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bioRxiv - Cell Biology 2024Quote: ... CytoLuc-pGL3 basic (Promega #E1751), pLVX-NEO-CMV-2mt-GCaMP6s and SNAP25-Aequorin were kindly provided by Dr ...
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bioRxiv - Cell Biology 2024Quote: All human cell lines were authenticated at each batch freezing by STR profiling (StemElite ID System, Promega). All cell lines were tested for mycoplasma at each batch freezing by PCR (32 ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa cells at 50 – 60% confluence were transfected using FuGene (Promega) according to Manufacturer’s instruction ...
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bioRxiv - Microbiology 2024Quote: 100,000 HEK-293T cells were co-transfected with 150 ng GFP/GFP-IpaH4/GFP-IpaH4C339S and 350 ng target vectors for 48 h using FuGENE (Promega; Table S4). Cells were harvested in RIPA buffer containing 100 µM PMSF and protease inhibitor cocktail (Abcam ...
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bioRxiv - Microbiology 2024Quote: ... Dual-luciferase reporter assays (Promega) were performed according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... the extracted RNA was reverse transcribed by using the reverse transcription system (Promega Corporation, Fitchburg, WI, USA). For this ...
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bioRxiv - Microbiology 2024Quote: LDH in neutrophil supernatants was quantified using the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega®) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... cell supernatants were harvested for luciferase activity detection using Nano-Glo Luciferase Assay System kit (Promega) on Glomax Multi-Detection System (Promega).
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bioRxiv - Microbiology 2024Quote: ... on Glomax Multi-Detection System (Promega).
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bioRxiv - Cancer Biology 2024Quote: ... cells were treated with increasing concentrations of CMLD012824 and analyzed using a RealTime-Glo Annexin V Apoptosis and Necrosis Assay kit (Promega JA1011) at 1 to 72 h ...
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bioRxiv - Cancer Biology 2024Quote: ... with 200U/mL RNAsin (Promega) (3x pellet volume ...
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bioRxiv - Cancer Biology 2024Quote: Rabbit reticulocyte lysates (RRL) (Promega L4960) were treated with micrococcal nuclease (NEB M0247S ...
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bioRxiv - Cancer Biology 2024Quote: ... Reactions were incubated for 1.5 hr at 30 °C and luciferase signal was measured using the Dual-Glo Luciferase Assay System (Promega E2920).
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bioRxiv - Microbiology 2024Quote: ... the reporter virus plasmid was linearized and in vitro transcribed to generate RNA using T7 RiboMax Express Large Scale RNA Production System (Promega). Transcribed RNA was purified using RNeasy Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... the lysates were diluted with 50mM HEPES pH8.0 to <2M urea and digested overnight with trypsin (Promega) at 1:50 (enzyme:protein ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 μl of cell lysate was added to 20 μl of Luciferase Substrate (Promega), rotationally mixed for 15 minutes at RT and luminescence quantified on a FLUOstar Omega plate reader (BMG Labtech) ...
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bioRxiv - Cancer Biology 2024Quote: ... then washed with 1 ml of PBS and lysed using 60 μl of Cell Culture Lysis Reagent (Promega). 20 μl of cell lysate was added to 20 μl of Luciferase Substrate (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... ATP-depleted uRBCs were then washed three times with PBS and ATP-depletion was evaluated for each dilution using the CellTiter-Glo→ Luminescent Cell Viability assay (Promega) following the instructions provided in the kit (see Extended Data Fig ...
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bioRxiv - Microbiology 2024Quote: Recombinant FIKK kinase domains activity was measured using the ADP-Glo kinase assay (Promega), which quantifies the amount of ADP produced during the kinase reaction ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was digested with ApaI and XhoI and then purified (Wizard® SV Gel, Promega, WI, USA). The eluted fragment was ligated downstream of an inducible acetoacetate decarboxylase promoter (Padc ...
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bioRxiv - Microbiology 2024Quote: ... DTT was then added to a final concentration of 3 mM before adding 1 μg of sequencing-grade trypsin (Promega) and incubating at 37C overnight ...
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bioRxiv - Cancer Biology 2024Quote: 1 microgram total RNA extracted using ReliaPrep™ RNA Cell Miniprep System (Promega, Madison, WI, USA), were checked using the Agilent-2100 Bioanalyzer System and subjected to library preparation utilizing the TruSeq stranded total RNA Gold library preparation kit with RiboZero Gold treatment and unique dual indexes according to the manufacturer’s instructions (Illumina Inc. ...
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bioRxiv - Cancer Biology 2024Quote: ... the CellTiter-Glo assay (Promega), was then performed according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... CellTitier-Glo assay (Promega) was performed according to manufacturer’s instructions at indicated days and the viability was normalized to day 0 or DMSO ...
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bioRxiv - Molecular Biology 2024Quote: ... Luciferase assays were carried out in using the GloMax Discover Microplate Reader (Promega).
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bioRxiv - Cancer Biology 2024Quote: ... and ABCB-CoChR-mCherry gene fragments were PCR amplified from pNL-CMV-NLuc (Promega #N1091), pcDNA3.0-Magneto2.0-p2A-mCherry ...
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bioRxiv - Cancer Biology 2024Quote: ... cell viability was measured using CellTiter-Glo Luminescent Cell Viability Assay (Promega, Madison, MI), as previously described(72).
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bioRxiv - Cancer Biology 2024Quote: ... and the CellTiter-Glo assay (Promega) was then performed according to the manufacturer’s instructions to determine cell proliferation ...
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bioRxiv - Microbiology 2024Quote: ... followed by addition of 100 ng porcine sequencing grade modified trypsin (Promega) in 100 mM ammonium bicarbonate (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: Assays were performed using Promega MTase-Glo Methyltransferase Assay (Promega V7601) with the following modifications ...
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bioRxiv - Microbiology 2024Quote: ... The master mix for the PCR reaction included 10 µL GoTaq Hot Start Green Master Mix (Promega, Madison, WI US), 2 µL of each primer at 10 µM ...
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bioRxiv - Cancer Biology 2024Quote: ... we supplemented culture medium with 10 μL of CellTiter96 Aqueous Non-Radioactive Cell Proliferation Assay (MTS) kit (Promega) reagent for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... the luciferase assay was performed using a luciferase assay kit from Promega following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... 20 μM of EnduRen live-cell substrate (Promega, E6481) was added to the cells in DMEM and incubated for at least 1 hour before detection using the Varioskan LUX Multi-well Luminometer (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... and simultaneously transfected with 0.4 μg of respective EGFP-Rab plasmids using FuGENE 6 transfection reagent (Cat. E269A, Promega, Madison, WI) per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Luciferase activity was measured in cell extracts using a Dual-Luciferase Reporter Assay Kit (Promega).
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bioRxiv - Microbiology 2024Quote: ... 1522bp using primers 5’-AAGGTACCTGAGGCTGGAGAGATGGCC-3’ and 3’-TAAAAGCTTCACCGGACTGGGCTAGTTCAG-5’ were PCR amplified and cloned in promoterless PGL3 enhancer empty vector (Promega, E1771) at the upstream of luciferase gene ...
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bioRxiv - Microbiology 2024Quote: ... mNeonGreen and Halotag fusion proteins were detected using polyclonal anti-mNeonGreen (NC, unpublished) and anti-Halotag (Promega) antibodies diluted at 1:10,000 and 1:2,000 respectively ...
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bioRxiv - Microbiology 2024Quote: ... 6μL Promega PCR amplification-grade water (Promega) and 2 μL of the sample DNA (corresponding to about 20 ng) ...
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bioRxiv - Microbiology 2024Quote: ... 4,7 μL of PCR amplification-grade water (Promega Corporation, Wisconsin, USA) and 2 μL of sample DNA (about 20 ng) ...
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bioRxiv - Microbiology 2024Quote: ... 40 μL of One-Glo-EX substrate (Promega) was added to the cells and incubated for at least 5 minutes on a plate shaker in the dark ...
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bioRxiv - Microbiology 2024Quote: ... Relative light units (RLU) were determined using the GloMax 20/20 Luminometer (Promega). GFP intensity was analyzed using a fluorescence microscope (Mshot ...
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bioRxiv - Molecular Biology 2024Quote: ... and gene expression was determined by quantitative PCR (QuantStudio 6 Pro Real-Time PCR System) with GoTag qPCR System (Promega, A6002). Actb and Rplp0 were used as housekeeping genes ...
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bioRxiv - Molecular Biology 2024Quote: ... Cholesterol depletion was validated using a Cholesterol/Cholesterol ester-glo assay (Promega: J3190). Endocytosis was blocked using two drugs Dynosore (selleckchem ...
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bioRxiv - Microbiology 2024Quote: ... the cells were lysed using passive lysis buffer (Promega). The transduction efficiency was quantified by measuring the GFP fluorescence in the prepared cell lysates using a Tecan infinite 200 pro plate reader ...
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bioRxiv - Microbiology 2024Quote: ... added same volume of CellTiter-Glo® Luminescent Cell Viability Assay reagent (Promega), and luciferase activity was measured using a BioTek Cytation 3 quipped with Gen5 software (v3.08).
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bioRxiv - Microbiology 2024Quote: ... the culture media were replaced with 50 μL of 1x Cell Culture Lysis Reagent (Promega) for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... The Steady-Glo Luciferase Assay System kit from Promega was used for all luciferase assays ...
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bioRxiv - Microbiology 2024Quote: ... or LDH-Glo Cytotoxicity Assay (Promega, J2380) were used to determine cell death ...