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Citations for Promega :
1451 - 1500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... Genomic DNA was isolated and purified using the Wizard Genomic DNA Purification Kit protocol for Gram negative bacteria at one-third volume (Promega), then quantified using SYBR Green I Nucleic Acid Stain (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... and primers were removed using the ProNex Size-Selective Purification System (Promega, NG2001) in a 1:2.4 (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... The fragment sizes provided by the Fragment Analyzer were compared to the degradation indices obtained from real-time quantitative polymerase chain reaction (qPCR) with PowerQuant System (Promega Corporation, Madison, WI, USA). After one year of storage in − 20 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... Standard curves based on 1:10 dilutions of 2800 M control DNA (Promega Corporation) (0.001 – 10 ng/µL ...
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bioRxiv - Genomics 2024Quote: ... and dual-luciferase activity was measured using the Dual-Glo Luciferase Assay kit (Promega) after a 48-h incubation with three biological replicates ...
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bioRxiv - Molecular Biology 2024Quote: Metabolic activity of CpdG or DMSO treated and oexORP7 or oexMYC cells was measured using CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega cat #: G358C), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were transfected using FuGENE 6 (Promega) with complementary DNA (cDNA ...
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bioRxiv - Microbiology 2024Quote: ... Amplifications were set up in a total volume of 20 μL containing: 10 μL GoTaq® Green Master Mix (Promega Corporation, Madison, Wisconsin, USA), 1 μL of each 10 μM primer ...
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bioRxiv - Microbiology 2024Quote: ... Amplicons were gel-purified using Wizard® SV Gel and PCR Clean-Up System (Promega) and quantified with a Qubit 4 Fluorometer (Thermofisher scientific ...
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bioRxiv - Microbiology 2024Quote: DNAase treatment using the kit RQ1 RNase-free DNase (Promega, ref. M6101) was performed ...
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bioRxiv - Microbiology 2024Quote: ... The beads were washed thrice with 180 µl 80 % (v/v) ethanol prior to the addition of the digestion enzyme mix (0.6 μg of trypsin (V5111; Promega, Madison, WI, USA) and 0.6 µg LysC (125-05061 ...
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bioRxiv - Microbiology 2024Quote: ... Amplicons were subsequently gel-extracted (Wizard SV Gel and PCR Clean-Up System, Promega), quantified ...
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bioRxiv - Microbiology 2024Quote: ... Luciferase was measured using Brioght-Glo reagent (Promega) approximately 18 hours post- infection ...
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bioRxiv - Microbiology 2024Quote: The genomic DNA was extracted with Wizard Genomic DNA Purification Kit (Promega). The Microbial WGS was performed using Illumina NovaSeq 6000 by Novogene (UK ...
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bioRxiv - Microbiology 2024Quote: ... Then 20 µL of 50 mM ABC containing 1 µg of trypsin (Promega Platinum Mass Spectrometry Grade) was added and the traps were incubated 2h at 47°C in a covered thermomixer without vortexing ...
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bioRxiv - Microbiology 2024Quote: ... supernatants containing trVLPs were collected and the remaining cells were lysed for luciferase assay using the 5x Promega Passive lysis buffer and luciferase activity was measured using a The Dual-Luciferase® Reporter (DLR™) Assay System (Promega, Madison, WI, USA). After confirming that firefly luciferase values remained consistent between conditions ...
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bioRxiv - Microbiology 2024Quote: ... ChIP eluates were purified with Wizard SV Gel and PCR clean-up system (Promega, Cat# A9282). Primer sequences are shown in Table S2 ...
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bioRxiv - Microbiology 2024Quote: ... Quantitative real-time PCR (qRT-PCR) was performed with the GoTaq polymerase (Promega) and a StepOnePlus real-time PCR device (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: The copy number of re-integrant strains was determined by extracting the genomic DNA and performing quantitative PCR (qPCR) using GoTaq polymerase (Promega) and a StepOnePlus real-time PCR machine (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: ... and anti-mouse IgG HRP conjugate (Promega, W4021) as the secondary antibody ...
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bioRxiv - Molecular Biology 2024Quote: ... the cells were transfected using Fugene (Promega) with a plasmid encoding the socRNAs of interest ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibodies (Anti-Rabbit/Mouse IgG HRP Conjugate, Promega) were used at 1:5000 in 5% Blotting-Grade Blocker (BioRad).
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bioRxiv - Microbiology 2024Quote: ... and the luciferase activities of the pseudoviruses were gauged using a luciferase assay system (E1501, Promega, USA).
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bioRxiv - Microbiology 2024Quote: ... or 4 μg of the pNL4.3-ΔEnv EGFP backbone36 (for viral entry assay in primary CD4+ T cells) into 293T cells using the FuGENE6 transfection reagent (Promega, USA). The cells were cultured at 37°C for 6 hours before the medium was completely replaced with fresh medium ...
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bioRxiv - Microbiology 2024Quote: ... after which cell viability was determined using the CellTiter-Glo Luminescent Cell Viability Assay (Promega). All treatments were performed in triplicate and repeated in three separate experiments.
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bioRxiv - Microbiology 2024Quote: ... The hydrolase activity of the UDP-substrate was detected as the release of UDP by the UDP-Glo assay (Promega) after 20 min of incubation with UDP-Glo detection reagent ...
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bioRxiv - Microbiology 2024Quote: The nucleotide sugar donor specificity of SdbA 510-1050 was assayed using the UDP- Glo Glycosyltransferase Assay (Promega) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... An aliquot of medium was assayed for extracellular LDH using the LDH-Glo Cytotoxicity Assay (Promega) to ensure that cell monolayers were not compromised by compound treatment ...
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bioRxiv - Microbiology 2024Quote: ... and luminescence from the activity of luciferase was measured at 48 h post-infection using a Tecan Infinite M200 Pro plate reader after applying Bright-Glo reagent (Promega) to the cells.
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bioRxiv - Microbiology 2024Quote: ... Proteins were then solubilized in 50 mM ammonium bicarbonate for a reduction-alkylation step (dithiothreitol 5 mM – iodoacetamide 10 mM) and an overnight digestion with 300ng of sequencing-grade porcine trypsin (Promega, Fitchburg, MA, USA). Digested peptides were resuspended in 0.1% formic acid (solvent A ...
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bioRxiv - Microbiology 2024Quote: ... with 8% FBS and 1.6% agarose (Promega). After 48 h ...
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bioRxiv - Microbiology 2024Quote: ... and subsequent digestion with sequencing-grade trypsin (Promega). To extract the resulting peptides ...
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bioRxiv - Microbiology 2024Quote: ... and luciferase activity was quantified utilizing a commercially available assay kit (Promega). In addition ...
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bioRxiv - Microbiology 2024Quote: ... and rehydrated with DNA Rehydration Solution (Promega) overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... 5 µl 5x optimized transcription buffer (Promega), 2 µl T7 RNA polymerase (20 U.ml-1 ...
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bioRxiv - Microbiology 2024Quote: ... 60 µM CTP (Promega, 10 mM stock) and 2-4 µl 10 mCi.ml-1 of radiolabelled CTP [α-32P] ...
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bioRxiv - Microbiology 2024Quote: ... which included a 5 µl nucleotide mix containing 2.5 mM NTPs (Promega). For each reaction ...
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bioRxiv - Microbiology 2024Quote: ... 5 µl of 5x optimized transcription buffer (Promega), 2 µl of T7 RNA polymerase (20 U.ml-1) ...
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bioRxiv - Microbiology 2024Quote: ... along with 1.5 µl of rRNasin (40 U.ml-1, Promega), 5 µl of 5x optimized transcription buffer (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... A non-template control consisting of nuclease free-water (Promega Corporation ...
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bioRxiv - Microbiology 2024Quote: ... 50 to 100 ng of template were used per reaction with 1.5 µl rRNasin 40 U.ml-1 (Promega), 5 µl 5x optimized transcription buffer (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... Lysis was completed by adding 5μL of nuclei lysis buffer (Promega) and 180μL of ddH2O ...
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bioRxiv - Microbiology 2024Quote: ... Lysis was completed by adding 10μL of nuclei lysis buffer (Promega) and diluting with 100μL of ddH2O ...
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bioRxiv - Microbiology 2024Quote: ... Complementary DNA (cDNA) synthesis was performed using GoScript™ Reverse Transcriptase (Promega) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing grade trypsin/lysC (Promega, Madison, WI) was added in 1:20 (wt/wt ...
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bioRxiv - Microbiology 2024Quote: ... (2) addition of 600μL of Nuclei Lysis Buffer (Promega), and (3 ...
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bioRxiv - Microbiology 2024Quote: Aliquots of the final populations were thawed and the gDNA was extracted using the Wizard Genomic DNA Purification Kit (Promega). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were precipitated through the addition of Protein Precipitation Solution (Promega) followed by vigorous vortexing ...
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bioRxiv - Molecular Biology 2024Quote: ... together with standard DNA ladders (1 Kb plus; Promega Corp.) to confirm amplification ...
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bioRxiv - Molecular Biology 2024Quote: ... the antigen was detected with the enhanced chemiluminescence (ECL) detection system (Promega) as specified by the manufacturer with Azure Sapphire Biomolecular Imager (Azure Biosystems ...