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Citations for Promega :
8901 - 8950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The 5’UTR region of the cyclin E gene was amplified from normal human peripheral blood lymphocytes genomic DNA using the Phusion High Fidelity DNA polymerase and was cloned downstream of the SV40 promoter and upstream of the firefly luciferase cDNA sequence in the pGL3 control vector (Promega, Madison, WI, USA). The DDX3X knockout HEK293FT cells were co-transfected with the Cyclin E-5’UTR luciferase reporter construct ...
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bioRxiv - Genomics 2023Quote: ... .pGEM-T easy cloning vector (Promega, Madison, WI, USA) was used for cloning ...
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bioRxiv - Biochemistry 2023Quote: ... The P450-Glo CYP1A1/1B1 assay kit and NADPH regeneration system and luciferase assay kit were purchased from Promega (Promega, Madison, WI) and utilized under the following conditions ...
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bioRxiv - Biochemistry 2023Quote: ... The P450-Glo CYP1A1/1B1 assay kit and NADPH regeneration system and luciferase assay kit were purchased from Promega (Promega, Madison, WI) and utilized under the following conditions ...
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bioRxiv - Cancer Biology 2023Quote: ... and RealTime-Glo Annexin V Apoptosis and Necrosis Assay (Promega), respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... CellTiter-Glo 2.0 Cell Viability Assay (Promega) and RealTime-Glo Annexin V Apoptosis and Necrosis Assay (Promega) ...
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bioRxiv - Cancer Biology 2023Quote: MSI status was determined using the MSI Analysis kit (MD1641, Promega) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: Luciferase DNA was constructed by cloning luciferase gene (luc2) (pGL4.13, Promega, Madison, WI), 120 adenine ...
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bioRxiv - Cell Biology 2023Quote: ... cells were assayed for luciferase activity using the dual luciferase assay kit (E1910, Promega, Madison, WI) as per manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... RNA (3μg) was converted to first strand cDNA using reverse transcriptase (Promega, United States) and oligo-dT ...
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bioRxiv - Biochemistry 2023Quote: ... 5 µL of 2x GoTaq (Promega, #A600A), 0,4 µL forward primer (0,5 µM) ...
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bioRxiv - Biochemistry 2023Quote: ... Rat Gβ1 was fused with a His-tag at the N terminus and with a SmBiT subunit (peptide 86, Promega)34 after a 15-amino acid linker at its C terminus ...
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bioRxiv - Biochemistry 2023Quote: ... Sequencing grade trypsin (Promega, WI) was diluted in HA buffer so that adding 2 μL of trypsin to each heat denatured aliquot resulted in a 1:100 molar ratio of trypsin to spike ...
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bioRxiv - Genomics 2023Quote: ... Resulting genomic DNA was assessed for concentration and purity using Promega DNA quantification kit on the Promega Quantus Fluorometer (Promega, Germany) and measurement of A260/A280 ratio on the MultiSkan Sky High spectrophotometer (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: ... containing RNase inhibitor RNasinTM (Promega). FACS analysis and sorting were performed on a (BD FACS Aria III ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit and mouse anti-β-Gal (MP Biomedics #559761 and Promega #Z378A, 1:1000), rabbit and mouse anti-pH3 (Merck Millipore #06-570 and Cell signal technology #9796 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA was isolated using the Wizard genomic DNA purification kit (Promega), and a PacBio library was prepared using the SMRTbell Express template prep kit (Pacific Biosciences) ...
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bioRxiv - Genetics 2023Quote: ... proteins in the plugs were digested overnight with trypsin (Promega) at 37°C in a 25-mM NH4HCO3 buffer (0.2 µg trypsin in 20 µL) ...
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bioRxiv - Genetics 2023Quote: ... Digestion was performed overnight at 37°C with 1 µg of trypsin (Promega). The tryptic digestion was stopped by lowering the pH of the reaction below pH 4 by adding extraction buffer (0.1% TFA 2% acetonitrile) ...
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bioRxiv - Genetics 2023Quote: ... and for 1 hour at room temperature in secondary antibody (1:4,000 anti-Mouse-HRP, Promega). In order to detect the endogenous Ptiwi09 protein ...
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bioRxiv - Genetics 2023Quote: ... Cells were lyzed and assayed for luciferase activity using the Dual Luciferase Reporter Assay System (Promega, Fitchburg, WI). Firefly luciferase activity was normalized to Renilla luciferase activity as relative light units (RLU) ...
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bioRxiv - Genomics 2023Quote: ... RNA concentrations were measured using the Qubit fluorometer (Promega). RNA integrity was evaluated using the Bioanalyzer system (Agilent ...
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bioRxiv - Synthetic Biology 2023Quote: ... or zero min at 37°C before addition of Nano-Glo substrate (Promega N1110) in 80 µL ...
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bioRxiv - Synthetic Biology 2023Quote: ... the Nano-Glo Luciferase Assay System (Promega N1110) was used ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were washed with PBS and harvested with 1X Passive Lysis Buffer (5X, Promega #E1941) diluted in water ...
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bioRxiv - Microbiology 2023Quote: ... Sequences encompassing the SD site and the env gene were acquired after cloning the PCR fragments inserted into pGEM-Teasy (Promega). Sanger sequencing with primers env7254(+ ...
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bioRxiv - Microbiology 2023Quote: ... Bright-Glo luciferase reagent (Promega) was added to each well ...
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bioRxiv - Physiology 2023Quote: ... The gDNA was isolated from cell pellets using the Wizard Genomic DNA Purification kit (Promega, A1120). Preparation and Illumina short read sequencing (PE 150 ...
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bioRxiv - Genetics 2023Quote: ... or the GloMax Explorer Multimode Microplate Reader (Promega, #GM3500). Relative luciferase activity (RLA ...
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bioRxiv - Immunology 2023Quote: ... luminescence was generated by adding 25 μL of Nano-Glo Luciferase Assay System (Promega). Spectramax M3 plate readers (Molecular Devices ...
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bioRxiv - Immunology 2023Quote: ... Eluted proteins were then treated with PNGase F (Promega) according to manufacturer’s protocol and analyzed with SDS-PAGE ...
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bioRxiv - Synthetic Biology 2023Quote: ... The Wizard Genomic DNA Purification Kit (Promega) was used for genome extraction ...
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bioRxiv - Synthetic Biology 2023Quote: ... strain ADP were extracted using Wizard™ Genomic DNA Purification Kits following the standard protocol provided by Promega.
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bioRxiv - Synthetic Biology 2023Quote: ... FluoroTectTM GreenLys tRNA was provided by Promega. Glyoxylic acid was purchased from Acros organics ...
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bioRxiv - Microbiology 2023Quote: ... and the input for the luciferase assay was normalized by using a CellTiter-Glo 2.0 assay kit (Promega) following the manufacturers’ instructions ...
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bioRxiv - Microbiology 2023Quote: ... a GloMax Explorer Multimode Microplate Reader 3500 (Promega) was used.
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bioRxiv - Immunology 2023Quote: RNA from purified splenic CD8 T cells was reverse transcribed into cDNA using oligo-d(T) primers and M-MuLV reverse transcription (Promega). Real-time quantitative PCR was performed using SYBR DNA polymerase (Thermo Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were prepared for the Bright-Glo Luciferase Assay according to the manufacturer’s protocol (Promega). Luciferase activity was detected by using a luminescence microplate reader (Cytation 5 ...
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bioRxiv - Biochemistry 2023Quote: NSD2 full-length template was cloned in frame into a pNLF1 vector (Promega) using the In-Fusion HD Cloning kit (Takara ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were lysed and assayed using the Dual-Luciferase® Reporter Assay System (Promega, E1910) per the manufacturors instructions ...
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bioRxiv - Microbiology 2023Quote: ... Porcine trypsin (1:50, w/w) (Promega) was added ...
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bioRxiv - Immunology 2023Quote: ... cells were co-transfected with 10µg TK-Renilla (#E2241, Promega) and 10µg HRE-luciferase (a gift from Navdeep Chandel ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were digested overnight on a 1000 rpm shaker at 37 ℃ with Trypsin Gold (Promega, Madison, WI, USA, Cat# V5820) in a 50:1 ratio (wt/wt ...
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bioRxiv - Bioengineering 2023Quote: ... Transfection was performed with 1 μg of plasmid DNA in combination with 3 μL of Fugene HD transfection reagent per well (Promega).
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bioRxiv - Cancer Biology 2023Quote: ... 100 µL of cells were plated in opaque-walled (white) multi-well plates and 100 µL CellTiter-Glo (Promega, WI, USA, G7572) solution was added avoiding bubbles ...
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bioRxiv - Bioengineering 2023Quote: ... alkylated with 55 mM iodoacetamide (IAA) and digested with the endopeptidase sequencing-grade Trypsin (Promega; Madison, WI, USA) overnight at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... in 25 mM NH4HCO3 (pH 8.0-8.5) and 0.01% ProteaseMAX w/v (Promega) for 16 hr at 37⁰C ...
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bioRxiv - Cancer Biology 2023Quote: Cell viability assays were performed using the RealTime-Glo™ MT Cell Viability Assay according to the manufacturer’s protocol (Promega, Madison, WI, USA). Cells were seeded in standard media in white-walled 96-well plates at a density of 5000 cells/well and were allowed to attach overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then incubated with Dual-Glo luciferase substrate (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... and cells using Maxwell 16 LEV Simply RNA Tissue Kit (Promega, Madison, WI, USA), according manufacturer’s protocol ...