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Citations for Promega :
8451 - 8500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... paraffin-embedded xenograft tumor tissue sections using DeadEnd Colorimetric TUNEL System (Promega) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with 20 μl of 4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) (AqueousOne, Promega) at 37°C for 1-2 hours for MTS colorimetric cell viability assay ...
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bioRxiv - Cancer Biology 2023Quote: ... the Nano-Glo® Live Cell reagent was reconstituted (Promega) and 25μL was added to the transfected cells ...
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bioRxiv - Cancer Biology 2023Quote: ... A quantification of organoid viability was performed using CellTiter-Glo 3D (Promega, #G9682) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... Halo-EGFR constructs used for FRET-FLIM experiments were created using the HA-EGFR plasmid and the Gibson assembly approach to replace the N-terminal HA tag with the HaloTag® plasmid (Promega, #G7711). The final resulting construct contained the HaloTag and a GSGS linker region after the EGFR signal sequence ...
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bioRxiv - Cancer Biology 2023Quote: ... The mice received intraperitoneal (IP) injection of 2 milligrams of luciferin (Promega Corporation, Madison, WI) and subjected to luminescence imaging using an IVIS® Spectrum In Vivo Imaging System (PerkinElmer Inc ...
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bioRxiv - Cancer Biology 2023Quote: ... Signals were detected with ECL Western Blotting Substrate (Promega Corp., Fitchburg, Wisconsin, USA), and immunoreactive bands were visualized using ChemiDoc Touch MP (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with 1× PBS and lysed with PLB according to the manufacturer’s instruction for 15 mins (Promega, E1910). Cells were transferred to a 96-well plate ...
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bioRxiv - Cancer Biology 2023Quote: ... cell viability was measured using CellTiter-Glo 2 reagent (Promega). After addition of the reagent to cell culture medium ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was detected using CellTiter Glo 2 reagent (Promega). After addition of the reagent to cell culture medium ...
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bioRxiv - Cancer Biology 2023Quote: ... The isolated RNAs (20 ng) were reverse-transcribed and quantitative PCR (qPCR) were perform using GoTaq Probe 1-Step RT-PCR system (Promega) in a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2023Quote: ... Authentication of cell lines was confirmed regularly by short tandem repeat (STR) profiling by using the GenePrint 10 kit (Promega, Madison, WI, USA) on an ABI 3730 DNA analyzer (Applied Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... The amplified PCR products were isolated and subcloned into pGEM-T easy vector (Promega) and sequenced using Bigdye terminator v3.1 (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2023Quote: The assessment of gene expression was performed by quantitative real-time Polymerase Chain Reaction (qPCR) using the GoTaq® qPCR Mix (Promega) in the 7500 Fast Real Timer PCR system (Applied Biosystems®) ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1:250 RNasin (Promega, #2515). Centrifugally clarified cell extracts were incubated with affinity medium (20 μl of slurry for αORF1p and αORF2p for 30 min at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: An MTS assay using the CellTiter 96® Aqueous One Solution (Promega, Madison) was performed to assess the rates of cell viability and proliferation in vitro ...
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bioRxiv - Cancer Biology 2023Quote: The activity of EBV miRs-BART 7-3p and 9-3p was evaluated in Akata-EBV/Cas9 cells by luciferase reporter gene assay with constructs based on the psiCheck-2 backbone (Promega). 3’-UTR sequences with miRNA-binding sites (Supplementary Material ...
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bioRxiv - Cancer Biology 2023Quote: ... according to the manufacturer’s protocol and the GloMax® Discover Microplate Reader (Promega).
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bioRxiv - Cancer Biology 2023Quote: ... into HEK293T cells in 57 µL Opti-MEM and 6 µL FuGENE HD (Promega, Fitchburg, WI, USA) transfection reagent as described (Gerstmeier et al ...
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bioRxiv - Cancer Biology 2023Quote: ... and 40 μg/mL streptomycin containing RealTime Glo reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... we used Caspase-Glo 3/7 and 8 assay systems (Promega). Approximately 2.0 × 103 cells/well were seeded in a 96-well plate and (1 ...
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bioRxiv - Cancer Biology 2023Quote: Cell proliferation was measured using CellTiter-Glo 3D (Promega G9683) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Caspase 3/7 activity was measured using the Caspase-Glo 3/7 Assay Kit (Promega G8091). Pre-treatment of cells with EGF ...
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bioRxiv - Cancer Biology 2023Quote: ... All cells were confirmed to be free of mycoplasma (PCR-based method) and authenticated by Short Tandem Repeat (STR) analysis using the GenePrint® 10 System (Promega, Madison ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were subjected to a CellTiterGlo cell viability assay (Promega), as described before36 ...
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bioRxiv - Cancer Biology 2023Quote: pRL-TK-renilla and pGL3 plasmids were obtained from Promega (Charbonnieres, France). pEF-cmyc-RIP140 and pEGFP-RIP140 have been previously described (31) ...
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bioRxiv - Cancer Biology 2023Quote: ... Firefly and Renilla signals were measured 24 h after transfection using the Dual-Glo Luciferase Assay System (Promega, cat#E2920) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was measured at day 4 and day 6 using the Cell-Titer Glo assay (Promega). Viability data was analyzed by comparing the relative viability change between base editing with the uORF gRNA and the associated parental CDS gRNA ...
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bioRxiv - Cancer Biology 2023Quote: ... and Quantus (Promega) instrument ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability in 3D was determined using CellTiter Glo (Promega, cat.#G9681) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was measured CellTiter-Glo reagent (Promega, Madison, WI) was measured at 16 hours post-transfection for a baseline assessment ...
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bioRxiv - Cancer Biology 2023Quote: ... and with fluorescence-based method using QuantiFluor Dye kit (Promega) and Quantus (Promega ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA and RNA were extracted from a single 10 μm curl using the Maxwell HT FFPE DNA Isolation System (Promega, Madison, WI) on the KingFisher™ Flex instrument (Thermofisher ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was assessed using a CellTiter cell proliferation assay kit (Promega), according to the instructions of the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stably transduced with a lentivirus-based expression construct generated by cloning a polymerase chain reaction (PCR)-amplified DNA fragment encoding luciferase from a pGL4.10-lucIIplasmid (Promega, Sydney, Australia) into a pLenti CMV Hygro DEST vector (Addgene ...
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bioRxiv - Cancer Biology 2023Quote: ... Viable cellularity was quantified at day 7 with CellTiter-Glo reagent (Promega) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Two replicate electroporations were each divided into three wells for 48h of culture and readout using the Dual-Glo Luciferase kit (Promega; E2940) in a Bio-Tek Synergy HT Microplate Reader.
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bioRxiv - Cancer Biology 2023Quote: ... after which viability was measured using the luminescence-based the CellTiter- Glo® Assay (Promega, G7571) on a SpectraMax iD3 or iDx Microplate Reader (Molecular Devices ...
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bioRxiv - Cancer Biology 2023Quote: ... Using FuGENE6 reagent (Cat. E2692; Promega), populations were transfected with TOP-FLASH or FOP-FLASH plasmid ...
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bioRxiv - Cancer Biology 2023Quote: TCF transcriptional activity was evaluated using a Luciferase Assay System (Cat. E1501; Promega) and TOP-FLASH ...
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bioRxiv - Cancer Biology 2023Quote: ... at 3,000 cells/well in 50 μL media containing IFN-α for 72 h prior to cell lysis with 50 μL CellTiterGlo 2.0 (Promega, G9243). The reaction was incubated in the dark for 20 min at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... PCTs were treated with the drug dosages specified in the main text and the viability was quantified by luminescent CellTiter-Glo® 3D assay (G9681, Promega). Representative images of PCTs viability were obtained at the confocal microscope using LIVE/DEAD™ Viability/Cytotoxicity Kit (L3224 ...
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bioRxiv - Cancer Biology 2023Quote: ... and cell culture identity verification was done by short tandem repeat profiling (GenePrint® 10 System, Promega #B9510).
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bioRxiv - Cancer Biology 2023Quote: ... by the dual luciferase assay system (Promega, Madison, WI,USA) following manufacturer’s instructions and as described before.39
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bioRxiv - Cancer Biology 2023Quote: ... Luminescence was measured using a Glomax Multi Plus Detection System (Promega, Madison, WI, USA), and caspase 3/7 activities was normalized by the number of cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Caspase-3 and -7 activities were measured using the Caspase-Glo 3/7 assay kit (Promega, G8090) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA concentration was measured using a Quantus fluorometer (Promega). Library preparation and 150bp paired-end sequencing with 450bp insert was conducted at Novogene.
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bioRxiv - Immunology 2023Quote: ... As per the manufacturer’s instructions (Promega, Cat# G3582) the absorbance at 490 nm was determined 1 hour following the addition of MTS and its inhibitory concentration 50 (IC50 ...
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bioRxiv - Cancer Biology 2023Quote: ... Luminescence was measured on the GloMax Discover (Promega, GM3000) and viability was normalized to 0 U/mL IFN control for each cell line.
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bioRxiv - Immunology 2023Quote: ... following the GoScript Reverse Transcription System protocol (Promega). Primers for Actb (Mm00607939 ...