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Citations for Promega :
8551 - 8600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... immunodepleted protein samples were successively digested with LysC (0.008 μg/μL, Wako) and trypsin (0.002 μg/μL, Gold, Promega), using a filter-aided sample preparation (FASP ...
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bioRxiv - Physiology 2023Quote: ... cDNA was synthesized using Oligo-dTs and M-MLV RT (Promega). qRT-PCR was performed with the StepOnePlus System (Applied Biosystems ...
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bioRxiv - Pathology 2023Quote: ... PCRs were performed in a final volume of 20 µL containing 1X of Colorless GoTaq® Flexi Buffer (Promega), 0.0625 mM of each dNTP ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Oligo dT(18)-primed reverse transcription was carried out with ImpromII Reverse Transcriptase (Promega). Semi-quantitative RT-PCR was carried out in the presence of [α-32P] dCTP (Perkin Elmer ...
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bioRxiv - Pathology 2023Quote: ... 0.4U of GoTaq® Flexi DNA Polymerase (Promega), 1.5 mM of MgCl2 (Promega ...
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bioRxiv - Physiology 2023Quote: ... the FuGENE 6 transfection reagent (Promega, WI, USA) was used to transfect wild-type (WT ...
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bioRxiv - Neuroscience 2023Quote: ... or T3 RNA polymerases (Promega) and purified through Micro Bio-Spin Columns with Bio-Gel (BIO-RAD) ...
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bioRxiv - Neuroscience 2023Quote: ... of neuropeptide and neurotransmitter marker genes were PCR-amplified from zebrafish brain cDNA or genomic DNA and cloned into the pGEM-T Easy vector (Promega). Three glutamate decarboxylase genes (gad1a ...
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bioRxiv - Neuroscience 2023Quote: HEK-293T cells were transfected with FuGene HD reagent (Promega # E2311) and PSP plasmid ...
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bioRxiv - Molecular Biology 2023Quote: ... and luminescence was measured by the Dual-Luciferase Reporter Assay System (Promega) and GloMax Navigator System (Promega).
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bioRxiv - Molecular Biology 2023Quote: ... cells were lysed with 100 μl of Passive Lysis Buffer (Promega). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... Histones were then digested with 1 µg of sequencing grade trypsin (Promega) diluted in 50mM ammonium bicarbonate (1:20 ...
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bioRxiv - Molecular Biology 2023Quote: ... 400 ng of total RNA was transcribed to cDNA using the ImProm-II™ reverse transcription kit (Promega, A3500) with random primers ...
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bioRxiv - Molecular Biology 2023Quote: ... and Renilla and Firefly luciferase activities were measured on a luminometer using the Promega dual luciferase kit (Promega, E1910) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: Genomic DNAs were purified from 600 µL of overnight bacterial culture using the Wizard HWM genomic DNA prep kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... EIL1-GST and GST proteins were synthesized using the TnT SP6 High-Yield Wheat Germ Protein Expression System (Promega, L3260), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Firefly LUC and REN activities were measured using the Dual-Luciferase Reporter Assay System (Promega, E1910) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... or rabbit anti chicken IgY HRP (Promega Corporation, G1351) 1:10,000] ...
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bioRxiv - Plant Biology 2023Quote: ... Reverse transcription was performed using 1μg total RNA and the M-MLV Reverse Transcriptase (Promega). qPCR was performed on a StepOne™ Real-Time PCR System (Thermo Fisher ...
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bioRxiv - Plant Biology 2023Quote: ... incubated with the secondary antibody rabbit anti chicken IgY HRP [(Promega Corporation, G1351) 1:10000] for 2 h and again washed three times ...
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bioRxiv - Plant Biology 2023Quote: ... In-gel digestion of proteins was performed at 37 °C overnight using 2.5 μg Trypsin/Lys-C (Promega). Samples were further digested with 2.5 μg Chymotrypsin (Promega ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were diluted 1:4 with 50 mM Tris/HCl (pH 8.0) and sequencing grade modified trypsin (Promega) was added in an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Plant Biology 2023Quote: ... Samples were further digested with 2.5 μg Chymotrypsin (Promega) at 25 °C for 4 hours ...
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bioRxiv - Plant Biology 2023Quote: ... After treatment with RQ1 RNase-Free DNase (Promega), the total RNA was reverse-transcribed using ReverTra Ace (Toyobo Life Science ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 20] using the T7 RiboMAXTM Express RNAi kit (Promega, USA) according to manufacturer’s instructions (Supplementary Table 2) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: CellTox assay (G8742, Promega, Leiden, Belgium) was used to assess survival of NIH-3T3 TrkB or PC12 cells under serum deprivation conditions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and incubated for 15’ with Proteinase K (1:500, in 1XPBS, Promega B.V., Leiden, The Netherlands) at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... NanoLuc luciferase activity in the lysates was measured using the Nano-Glo Luciferase Assay System (Promega) with a Tecan Infinite microplate reader ...
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bioRxiv - Physiology 2023Quote: ... Complementary cDNA was synthetized using M-MLV Reverse transcriptase (Promega). Messenger RNA (mRNA ...
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bioRxiv - Physiology 2023Quote: ... primers at a final concentration of 300nM and 6µL of GoTaq® qPCR Mix (Promega, Madison, USA). qPCR conditions were the following ...
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bioRxiv - Systems Biology 2023Quote: ... 40 µl PBS and 40µl Bright-GloTM luciferase assay reagent (Promega, E2650) were added per well ...
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bioRxiv - Systems Biology 2023Quote: Intracellular GSH/GSSG was measured using GSH/GSSG-GloTM luminescent assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: Cell viability was measured using a commercial kit (Cell Titre 96 AQueous One Solution cell proliferation assay; Promega, Madison, USA) that uses a tetrazolium salt-based colorimetric assay [20] ...
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bioRxiv - Plant Biology 2023Quote: ... Protein for the 4-methylumbelliferyl-b-D-glucuronide (MUG) and luciferase (LUC) assays was extracted in 1x passive lysis buffer (PLB: Promega). MUG assays were performed by adding 40 mL of protein extract to 100 mL of MUG assay buffer [2 mM MUG ...
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bioRxiv - Plant Biology 2023Quote: ... LUC activity was measured with 20 mL of protein sample and 100 mL of LUC assay reagent (Promega). Promoter activation was calculated as (rate of MUG accumulation / LUC luminescence ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were digested in-gel using trypsin (modified, sequencing purity, Promega), as previously described [88] ...
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bioRxiv - Plant Biology 2023Quote: ... and luciferase activities were measured using a dual-luciferase reporter assay kit (Promega, Beijing China).
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bioRxiv - Plant Biology 2023Quote: ... The recombinant baculovirus was generated using FuGENE HD Transfection reagent (Promega) in Sf9 cells using Insect X-press (Lonza ...
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bioRxiv - Plant Biology 2023Quote: ... following the DNA extraction protocol published by (Mahuku 2004) and further purified with a DNA purification kit (PROMEGA). The quantity and quality of DNA were confirmed by electrophoresis on agarose gels stained with GelRed and visualized under UV light using a NanoDrop 1000 spectrophotometer (Thermo Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... RNA was reverse-transcribed into cDNA using ImProm-II™ Reverse Transcription System (Promega, WI, USA). Real-time PCR was conducted using the SYBR green PCR reagent (Roche ...
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bioRxiv - Synthetic Biology 2023Quote: ... Firefly and Renilla luciferase activities were then measured sequentially on a Pherastar using the Dual-Glo Luciferase Assay (Promega, catalog #E2920) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: The hygromycin CDS of pGL4.33[luc2P/SRE/Hygro] (Promega) was replaced with a Renilla luciferase CDS via Gibson assembly using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Synthetic Biology 2023Quote: Cleavage by TEV protease was performed using ProTEV Plus (Promega) with 1 mM DTT ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli by miniprep (PureYield™ Plasmid Miniprep System, Promega), ethanol precipitated ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 μL of stopped reaction samples were mixed with 50 μL of luciferase substrate solution (Luciferase Assay System kit, Promega) in a white flat bottom 96-well microplate ...
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bioRxiv - Synthetic Biology 2023Quote: ... Reactions were set using the RiboMAX™ Large Scale RNA Production System (Promega) and incubated at 37ºC overnight ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli S30 Extract System for Circular DNA (Promega). The second kit was only used for the T7 RNA polymerase circuit ...
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bioRxiv - Systems Biology 2023Quote: ... containing 0.5 µg Trypsin (Promega), 0.1 µg Lysyl Endopeptidase (FUJIFILM Wako ...
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bioRxiv - Systems Biology 2023Quote: ... 0.1 µg Lysyl Endopeptidase (FUJIFILM Wako) and 0.01% ProteaseMaxTM (Promega) in 20 mM ABC was added ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... and luciferase activity was recorded using the Promega Luciferase assay system (Promega #E4030) according to the manufacturer’s instructions and as published45 using a TECAN reader (SPARK®TECAN) ...