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Citations for Promega :
8501 - 8550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and 160U GoScript Reverse Transcriptase (Promega #A5004) was then added to each well ...
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bioRxiv - Immunology 2023Quote: ... Baculoviruses were generated by transfecting purified bacmid DNAs into Sf9 cells using FuGENE HD (Promega), and subsequently used to infect suspension cultures of High Five cells (Life Technologies ...
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bioRxiv - Immunology 2023Quote: ... 1% Triton X-100) and Bright-Glo (Promega Corporation E2620) at a 1:10 ratio ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids were stained using Hoechst 33342 (1 μg/mL) and CellTox Green (Promega, 1/20 000) dyes for 8 h prior to imaging at Opera Phenix (Perkin Elmer ...
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bioRxiv - Immunology 2023Quote: ... at the indicated time points media was removed and a CellTiter-Glo assay (Promega) was completed following manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... The culture was diluted 1:100 in fresh LB supplemented with chloramphenicol and kanamycin including a 1:5000 dilution of the Nano-Glo® Luciferase Assay Substrate (Promega). Preliminary growth curves in the presence of the substrate showed no impact on growth at this concentration ...
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bioRxiv - Microbiology 2023Quote: ... AMV-RT (Promega) was used to reverse-transcribe the RNA-protein complex reaction at 30 °C for 1 h ...
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HIVtat Alters Epithelial Differentiation State and Increases HPV16 Infectivity in Oral KeratinocytesbioRxiv - Microbiology 2023Quote: ... 200 ml of media was treated with 4 U of RQ1 RNase-free DNase (Promega) for 1 hour at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... sequencing reaction (fmol kit, Promega) using the same end labeled primer was run to identify the exact position of RT stops.
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bioRxiv - Microbiology 2023Quote: ... goat α-mouse IgG antibodies (Promega) and donkey α-goat IgG (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... alkylated with iodoacetamide and digested overnight with trypsin (Promega Mannheim, Germany). Resulting peptide mixtures were extracted twice by exchange of 5% of formic acid (FA ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was washed twice with 1X TBS-TT (10X TBS-TT is 10X TBS buffer with 5 mL/L Tween 20 and 5 mL/L Triton X) and once with 1X TBS before incubation with the secondary antibody (IgG anti-rabbit HRP conjugate, Promega) 1:1,000 diluted in blocking solution for 1 hr ...
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bioRxiv - Microbiology 2023Quote: ... The Wizard® Genomic DNA Purification Kit (Promega Corporation, WI, USA) was used to extract the genomic DNA according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... firefly luciferase signal of HEK-NF-κB_luc cells was determined using Steady Glo/Bright Glo Luciferase Assay (Promega, USA), as previously described (12) ...
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bioRxiv - Microbiology 2023Quote: ... Gene expression was assessed by qPCR using primers listed in the supplemental material Table S6 and the GO Taq PCR kit (Promega). Gene expression was quantified using SYBR Green (Roche ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was isolated using the Wizard genomic DNA purification kit (Promega) according to manufacturer’s protocol ...
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HIVtat Alters Epithelial Differentiation State and Increases HPV16 Infectivity in Oral KeratinocytesbioRxiv - Microbiology 2023Quote: ... Total RNA was incubated with RQ1 RNase-free DNase (Promega) for 45 minutes at 37° C prior use in reverse transcription reactions ...
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HIVtat Alters Epithelial Differentiation State and Increases HPV16 Infectivity in Oral KeratinocytesbioRxiv - Microbiology 2023Quote: ... Blots were washed twice with 1X PBS-T followed by incubation for 1 hour with HRP-conjugated secondary antibody (Promega) diluted 1:10,000 in PBS-T ...
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bioRxiv - Microbiology 2023Quote: ... HaloTag staining was conducted with the HaloTag TMR Ligand according to the manufacturer’s instructions (Promega). Confocal images were acquired with a Zeiss ConfoCor 2 (63× ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... Cell pellets were resuspended in 70 μL of a 50/50% mixture containing PBS and the Caspase-Glo 3/7 reagent (Promega). Lysates were incubated at least 2 hours protected from the light at room temperature ...
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bioRxiv - Microbiology 2023Quote: This was assessed using the CellTiter-Glo® reagent (Promega, Cat. No. G7572) to measure levels of ATP as a real-time indicator of parasite viability.48 Test compounds were serially diluted in DMSO across eight concentrations ranging from 4 μM to 0.87 nM ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... and the protein solution was digested with trypsin (Promega) overnight at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Blots were washed twice with 1X PBS-T followed by incubation for 1 hour with HRP-conjugated secondary antibody (Promega) diluted 1:10,000 in PBS-T ...
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bioRxiv - Microbiology 2023Quote: ... 5 μL GoTaq Colorless Master Mix (Promega Corporation, Madison, Wisconsin) including 200 μM of each dNTP and 1 unit Taq DNA polymerase ...
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bioRxiv - Microbiology 2023Quote: ... viral RNA was quantified by real-time RT-qPCR (GoTaq 1 step RT-qPCR kit, Promega). Quantification was provided by serial dilutions of an appropriate RNA standard ...
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bioRxiv - Microbiology 2023Quote: ... 30 μg of the total RNA was firstly digested with RQ1 DNase (Promega, WI, USA), and then reverse transcribed into complementary DNA (cDNA ...
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bioRxiv - Microbiology 2023Quote: ... and then reverse transcribed into complementary DNA (cDNA) using 30 enzyme units(U) of the MMLV-RT (Promega, WI, USA) and 1μM of the labeled primer ...
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bioRxiv - Microbiology 2023Quote: ... RNA was reverse transcribed into complementary DNA using M-MLV Reverse Transcriptase (Promega, MA, USA). qPCR assay was prepared using KAPA SYBR® FAST qPCR Kit (Kapa Biosystems ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... cDNA (50 μL) was synthesized using a Reverse Transcription System Kit (Promega). Expression of the RNA-dependent RNA polymerase (RdRP ...
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bioRxiv - Microbiology 2023Quote: Cell viability was measured using the CellTiter-Glo Luminescent Cell Viability Assay (Promega). Cells were seeded in opaque-walled clear 96 well plates (Corning ...
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bioRxiv - Microbiology 2023Quote: ... cells were lysed using the passive lysis buffer supplied by the Dual-Luciferase® Reporter Assay System (Promega, #E1910). Measurement of firefly luciferase activity by adding LAR II ...
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bioRxiv - Microbiology 2023Quote: ... Luminescence was measured using the GloMax Explorer Reader (Promega) with an integration time of 0.3 secs per well ...
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bioRxiv - Microbiology 2023Quote: ... RNase inhibitor (Promega, #N2611); Streptavidin (Invitrogen™ ...
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bioRxiv - Microbiology 2023Quote: ... 2.5 μl of translation reaction products and 50 μl of Luciferase Assay Reagent (Promega) were mixed by gently pipetting and subjected to luminometer reading (Cytation 5 imaging multimode reader ...
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bioRxiv - Microbiology 2023Quote: ... 1uL RNasin Ribonuclease Inhibitor (Promega, N2511), 1uL TGIRT (InGex ...
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bioRxiv - Microbiology 2023Quote: ... 100μL luciferase assay substrate (Promega, Southampton, UK) was added to 20μL of the thawed cell lysate in white 96 well plates ...
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bioRxiv - Microbiology 2023Quote: ... and the protein solution was digested with 0.5 µg trypsin (Promega) overnight at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... After 16 h cells were lysed in 1x passive lysis buffer (Promega) and firefly luciferase activity was measured after addition of firefly substrate-containing buffer (20 mM Tricine ...
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bioRxiv - Microbiology 2023Quote: ... the ladder region was cut off and stained for 30 min in the running buffer containing 10000x diluted Diamond™ Nucleic acid dye (Promega). The transfer of the RNA from the agarose gel to the nylon membrane (Amersham Hyband™-N+ ...
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bioRxiv - Microbiology 2023Quote: ... All RNA extractions were performed on a Maxwell 16 instrument (Promega) with the Maxwell RSC Viral Total Nucleic Acid Purification Kit and eluted in 50uL of nuclease-free water ...
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bioRxiv - Microbiology 2023Quote: ... Total genomic DNA was isolated from overnight cultures of Ag1 and Ag2 using the Genome Wizard kit (Promega, WI), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... cDNA was generated via reverse transcription using the GoScript Reverse Transcription System (Promega) with 1 ul GoScript RT and 250 ng of RNA in a total volume of 20ul followed by incubation at 25 °C for 5min ...
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bioRxiv - Genomics 2023Quote: qRT-PCR was performed using GoTaq qPCR Master Mix (Promega) per the manufacturer’s in-structions on a CFX Connect Real-Time PCR system (BioRad ...
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bioRxiv - Microbiology 2023Quote: ... using the CellTiter-Glo Luminescent Viability Assay kit (Promega).
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bioRxiv - Microbiology 2023Quote: ... The luciferase enzymatic activities were measured using the Renilla Luciferase Assay system (Promega) and a Centro XS3 luminometer (Berthold ...
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bioRxiv - Microbiology 2023Quote: ... Luciferase activity was measured at 24 hours post-infection (hpi) using the Nanoluc Luciferase kit (Promega) and a Centro XS3 luminometer (Berthold) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and the quantity was measured by a Quantus fluorometer (Promega).
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bioRxiv - Evolutionary Biology 2023Quote: ... Quality was assessed with an Agilent Bioanalyser and the quantity was calculated by an RNA-specific kit from Quantus (Promega).
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bioRxiv - Cell Biology 2023Quote: ... Libraries were amplified using GoTaq (Promega), with 20 cycles for PCR1 and 15 cycles for nested PCR2 on 50% material from 1st PCR ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... GloSensorTM cAMP Assay Reagent (Promega, E1290), ZeocinTM Selection Reagent (ThermoFisher Scientific ...