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Citations for Promega :
8651 - 8700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... followed by blotting with HRP-conjugated secondary antibodies (Promega). The protein bands were obtained with the ChemiDoc XRS+system (Bio-Rad ...
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bioRxiv - Bioengineering 2023Quote: ... and M-MLV reverse transcriptase (Promega). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Bioengineering 2023Quote: ... mRNA was reverse-transcribed to cDNA by using oligo-dT primer (Promega) and M-MLV reverse transcriptase (Promega) ...
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bioRxiv - Immunology 2023Quote: ... Levels of infection was measured with the Bright-Glo luciferase kit (Promega) on a Victor X3 multilabel reader (Perkin Elmer) ...
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bioRxiv - Cancer Biology 2023Quote: ... Luciferase gene expression in E0771 and RM-1 cells was validated using a luciferase activity assay kit (Promega). Luciferase-labeled E0771 and RM-1 cells were maintained in RPMI 1640 media supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: Proteolytic activity was assessed using a Proteasome-Glo™ assay system (Promega, G8531), comprising Proteasome-Glo™ Chymotrypsin-Like Assay (G8621) ...
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bioRxiv - Cancer Biology 2023Quote: ... cell viability was measured using CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega, G3582).
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bioRxiv - Cancer Biology 2023Quote: ... Cells were lysed with 100μL of lysis buffer provided with the Dual-Glo Luciferase assay kit (Promega). The GloMax® 96 Microplate Luminometer was used to measure the activity of Firefly and Renilla luciferases according to the manufacturer’s protocol (Promega) ...
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bioRxiv - Cancer Biology 2023Quote: ... The GloMax® 96 Microplate Luminometer was used to measure the activity of Firefly and Renilla luciferases according to the manufacturer’s protocol (Promega). For each sample ...
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bioRxiv - Cancer Biology 2023Quote: ... and pGL3-Control (Promega, E1741) or pGL3-IFNB (Addgene ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were transfected with pRL-SV40 (Promega, E2231) and pGL3-Control (Promega ...
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bioRxiv - Cancer Biology 2023Quote: Luciferase assays were performed using Promega Dual-Glo Luciferase Assay system (Promega, E2920), following manufacturer protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was determined using the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (MTS) kit (Cat. #G3580, Promega) at 24 ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by addition of 25 uL/well of Antibody Dependent Cellular Cytotoxicity (ADCC) Bioreporter Cells (Promega cat# G7010). The ADCC Bioreporter cells are engineered Jurkat cells stably expressing FcγRIIIa V158 (high affinity ...
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bioRxiv - Cancer Biology 2023Quote: ... 25 μL of CellTiter-Glo reagent (Promega #G9241) was added to each well using a Multidrop Combi to measure viability ...
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bioRxiv - Cancer Biology 2023Quote: ... First-strand complementary DNA (cDNA) synthesis was performed using 2 μg of total RNA with M-MLV reverse transcriptase (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were digested with Lys-C (Wako) and sequencing-grade trypsin (Promega, V5117) (1 μg of each in 20 μL of 50 mM TEAB ...
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bioRxiv - Microbiology 2023Quote: ... The amplified products were cloned into the pGEM-T Easy vector (Promega) and subcloned into the pAE expression vector [55] ...
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bioRxiv - Microbiology 2023Quote: ... using the Luciferase Assay System (Promega, Madison, WI, United States) according to manufacturer’s instructions.
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested by addition of 1 µg of Trypsin (Promega) and incubated overnight at 37 °C at 600 rpm on a thermoshaker ...
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bioRxiv - Genetics 2023Quote: ... pNL1.1PGK[Nluc/PGK] (Promega #N1441) was used as transfection control ...
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bioRxiv - Genetics 2023Quote: ... Luciferase activity was assessed 48h post-transfection (72h post siRNA transfection) using the Nano-Glo Dual-Luciferase Reporter Assay System (Promega) in a Synergy 2 Multi-Mode Microplate Reader (BioTek) ...
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bioRxiv - Genetics 2023Quote: ... The TK promoter from pGL4.54[luc2_TK] (Promega #E5061) was cut with KpnI and HindIII ...
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bioRxiv - Genetics 2023Quote: ... The pGL4.54[luc2_TK] (Promega #E5061) was used as transfection control ...
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bioRxiv - Genetics 2023Quote: ... Luciferase activity was assessed 48h post-transfection using the Nano-Glo Dual-Luciferase Reporter Assay System (Promega) in a Synergy 2 Multi-Mode Microplate Reader (BioTek) ...
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bioRxiv - Genetics 2023Quote: ... the PGK promoter from pNL1.1.PGK[Nluc/PGK] (Promega #N1441) was removed by digestion with KpnI and HindIII ...
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bioRxiv - Genetics 2023Quote: ... 12.5μl of GO Taq green master mix (Promega) and 2μl of template DNA (average concentration = 30ng/μl) ...
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bioRxiv - Immunology 2023Quote: ... stable HEK293 EBNA cell lines were generated using the sleeping beauty transposon system.91 Expression constructs were co-transfected with a transposase plasmid (10:1) into the HEK293 EBNA cells using FuGENE® HD transfection reagent (Promega GmbH, Madison, USA) in DMEM/F12 supplemented with 6% FBS ...
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bioRxiv - Immunology 2023Quote: ... dNTP mixture (Promega Corp., Madison, WI, USA.), and Oligo dT primers (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Nanoluciferase activity was measured using the Nano-Glo Luciferase Assay System (Promega, catalog #N1110) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Retroviral producer cell lines were generated by transiently transfecting of H29 cells with named constructs utilizing standard CaPO4 transfection (Promega, Madison, WI, USA). Supernatant from transfected H29 cells was used to transduce Phoenix-ECO (pECO ...
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bioRxiv - Immunology 2023Quote: T-cell and prodrug cytotoxicity assays with secreted enzymes were performed after 24 to 48 hours using CellTiter-Glo (Promega, Madison, Wisconsin, USA) or luminescence using firefly (ffLuc ...
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bioRxiv - Evolutionary Biology 2023Quote: ... one microliter volumes of Reverse Transcription reactions were used as template for amplification of internal cDNA fragments using in 20 μl volume PCR reactions (35 rounds of amplification using Promega 2X PCR Master Mix (Promega, Madison, WI). Gene-specific primers and annealing temperatures are listed in Table 1 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... post-injection using a Synergy HTX Multi-mode Microplate Reader (Bio Tek Instruments, Winooski, VT) and the Nano-Glo Dual Luciferase Reporter System (Promega, Madison, WI) as per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... and digested with trypsin/Lys-C mix (V5071, Promega, Madison, WI). The resulting peptides were cleaned up with Acclaim™ PepMap™ 100 C18 spin columns (SEM SS18V ...
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bioRxiv - Developmental Biology 2023Quote: ... and transcription with T7 RNA polymerase (Promega, Madison, WI).
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bioRxiv - Genetics 2023Quote: ... 36 hours after transfection firefly and renilla luciferase activities were measured using the Dual- Luciferase Reporter Assay System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... The firefly luciferase constructs were co-transfected with the Renilla luciferase vector (Promega) in 1-2X10^6 LCLs at a ratio of 2ug:40ng respectively ...
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bioRxiv - Genetics 2023Quote: ... The assay was performed on fresh leaf disks collected 72 hours after Agrobacterium-mediated infection and following the manufacturers instruction (Promega). A reference vector overexpressing the Renilla reniformis luciferase (REN ...
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bioRxiv - Microbiology 2023Quote: ... phage genome was extracted using Wizard genomic DNA purification kit (Promega, Madison, WI) and sequenced at Massachusets General Hospital next generation sequencing core facility ...
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bioRxiv - Microbiology 2023Quote: ... in the presence of ProteaseMax (Promega) overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... Slides were incubated in freshly made stain solution (5mM potassium ferricyanide + 5mM potassium ferrocyanide + 1mg/mL X-gal (Promega cat no. V3941) in wash buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... then incubated in freshly made stain solution (5mM potassium ferricyanide + 5mM potassium ferrocyanide + 1mg/mL X-gal (Promega cat no. V3941) in wash buffer ...
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bioRxiv - Cell Biology 2023Quote: ... and 1μg total DNA:4μL of Fugene (Promega) per well ...
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Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: ... GoTaq qPCR master mix (Promega A6001) was used in a final reaction volume of 20 µl ...
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bioRxiv - Developmental Biology 2023Quote: ... mouse anti-beta galactosidase (Promega Z378825580610; 1:500), guinea pig anti-brk (provided by from Gines Morata ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Multiplex PCRs were modified using 5X GoTaq Flexi colorless reaction buffer (Promega Corp., Madison, USA) instead of 1X and performed using a T100™ Thermal Cycler (Bio-Rad Laboratories Inc.).
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bioRxiv - Evolutionary Biology 2023Quote: ... Amplicons were cloned into the pGEM-T vector (Promega, USA) and their identity confirmed using Sanger sequencing ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Finally embryos were placed in BCIP-NBT (Promega) with AP buffer to react ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Probe synthesis reactions were performed at 37 °C for 3 h and then were treated with DNase I (Promega) at 37 °C for 20 min ...