Labshake search
Citations for Promega :
7901 - 7950 of 8279 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... Their identity was confirmed through cell line authentication carried out by BMR Genomics (Italy) by amplification of 23 STR loci (PowerPlex Fusion System kit, Promega). Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Bioengineering 2023Quote: ... PCR products were confirmed on a 0.8% agarose gel and purified using Wizard® SV Gel and PCR Clean-Up kit (Promega). DNA fragments were quantified using Nanodrop (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA was isolated from peripheral blood samples using the Maxwell RSC Blood DNA kit (Promega, Cat# AS1400 using the Maxwell RSC benchtop automated DNA/RNA extraction instrument (Promega). Total CER-1236 T cells present in the blood was determined using ddPCR as previously described (17) ...
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bioRxiv - Cell Biology 2023Quote: ... Necrosis was quantified by measuring release of lactate dehydrogenase in 30 µl samples of the medium and apoptosis by determining cell-associated Caspase3/7 activity using the respective kits from Promega Corp. ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was isolated from 30 mg of ground plant tissue using the SV Total RNA Isolation System Kit (Promega) and its concentration and integrity were assessed using the RNA 6000 Nano Kit on a Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Biochemistry 2023Quote: ... Authentication of this cell line was performed by the Johns Hopkins Genetics Resources Core Facility by short tandem repeat analysis using the PowerPlex 16 HS kit (Promega) within a year of study ...
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bioRxiv - Microbiology 2022Quote: ... We extracted genomic DNA from whole blood using a Wizard® Genomic DNA Purification Kit (#A1120, Promega Corp., Madison, USA) and then performed IPATS-BLV ...
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bioRxiv - Genetics 2022Quote: ... we added 25 uL Dulbecco’s PBS and 25 uL of reagent 1 from the Dual-Glo luciferase kit (Promega Inc.) and mixed well to lyse the cells ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was purified from the gel fragments using the Wizard SV Gel and PCR Clean Up kit (Promega, Fitchburg, USA) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: ... sequence was amplified from human genomic DNAs and used as in vitro transcription template in the reaction using Riboprobe System-T7 Kit (P1440, Promega) in the presence of 0.4μL 5-(3-Aminoallyl)-uridine-5’-triphosphate labeled with ATTO 680(Aminoallyl-UTP-ATTO-680 ...
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bioRxiv - Developmental Biology 2022Quote: ... the T7-VEE constructs were digested with Mlul and then in vitro transcribed using RiboMAX Large Scale RNA Production System Kit (Promega). The transcripts were 2’-O-methylated ...
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bioRxiv - Genomics 2022Quote: ... A single colony was used to inoculate LB Broth and genomic DNA extracted from 1 mL using a Promega Wizard kit according to the manufacturer’s instructions (Promega, USA). DNA was quantified using the Qubit 3 and Nanodrop (Thermofisher ...
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bioRxiv - Physiology 2022Quote: ... cDNA was synthesized by using qScript cDNA Synthesis Kit (QuantaBio, Beverly, MA) and PCR was performed with GoTaq qPCR Master Mix (Promega) with HPRT as the control gene ...
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bioRxiv - Microbiology 2022Quote: ... Approximately 4 µg of the extracted material was then used as template to in vitro synthesized viral genomic RNA transcripts using the Ribomax T7 RNA transcription Kit (Promega) and Ribo m7G Cap Analogue (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... The firefly luciferase activity was detected in homogenates of organs by luminescence measurements using Synergy H4 hybrid reader (Bio-Tek) and Bright-Glo Luciferase Kit (Promega).
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bioRxiv - Synthetic Biology 2022Quote: ... and plasmids were extracted from individual cultures outgrown in LB/ampicillin (50 μg/mL) using the PURE Yield Plasmid Miniprep kit (Promega). Individual clones were screened and confirmed by Sanger sequencing at Macrogen-Europe B.V ...
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bioRxiv - Plant Biology 2022Quote: ... The DAP-seq gDNA library was prepared using KAPA HiFi HotStart ReadyMix with unique index primers. ZmEREB57 was fused to HaloTag using the kit from pFN19K HaloTag T7 SP6 Flexi Vecto (cat. no. G184A) (Promega). ZmEREB57 fused to HaloTag was expressed using the TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260 ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was then synthesized using 2 μg of mRNA and a Transcriptase first-strand cDNA synthesis kit (Promega, Madison, WI). Real-Time qPCR to detect the indicated targets was performed on a LightCycler® 480 System (Roche Life Science ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells were transiently transfected with plasmid DNA pHIV-1 NL4·3Δenv-Luc and Spike-Δ19-D614G by using profection mammalian transfection kit (Promega Inc.). After 48 hours ...
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bioRxiv - Genomics 2022Quote: ... DNA used for microarray was isolated from frozen cell pellets (3×106-7×106 cells) using the Maxwell RSC Cultured Cells DNA Kit on a Maxwell RSC 48 instrument (Promega). DNA was genotyped at the Children’s Hospital of Philadelphia’s Center for Applied Genomics using the Infinium Omni2.5-8 v1.3 BeadChip (Illumina ...
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bioRxiv - Biochemistry 2023Quote: ... HiBit and firefly luciferase activity were measured simultaneously using a Nano-Glo HiBiT Dual-Luciferase Reporter System kit (Promega, N1630) with purified LgBiT Protein (Promega) ...
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bioRxiv - Biochemistry 2023Quote: ... PCR reaction mixtures were solved in 1% (w/v) agarose gel and purified using Wizard SV gel & PCR Clean-Up kits (Promega). Then ...
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bioRxiv - Cancer Biology 2022Quote: ... for 72 hours and cell proliferation was assayed using the CellTiter 96 Non-Radioactive Cell Proliferation kit and a GloMax Discovery plate reader (Promega). For proteomic mass spectrometry ...
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bioRxiv - Cancer Biology 2023Quote: ... All cell lines were validated by DNA fingerprinting through the University of Colorado Molecular Biology Service Center utilizing the STR DNA Profiling PowerPlex-16 HS Kit (DC2101, Promega)(Extended Data Figure 11).
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bioRxiv - Genomics 2023Quote: DNA was extracted from the agar/cell mixture from the output and control pools using a Wizard Genomic DNA Purification Kit (Promega). The manufacturer’s protocol was followed with two modifications ...
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bioRxiv - Biochemistry 2023Quote: ... U87MG cells were authenticated within a year of their use by the Johns Hopkins Genetics Resources Core Facility by short tandem repeat analysis using the PowerPlex 16 HS kit (Promega). Unless otherwise indicated ...
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bioRxiv - Microbiology 2023Quote: Genomic DNAs were purified from 600 µL of overnight bacterial culture using the Wizard HWM genomic DNA prep kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was extracted from backup samples for transcriptome analysis using Eastep® Super Total RNA Extraction Kit (Promega, USA). Reverse transcription was performed using GoScriptTM Reverse Transcription System (Promega ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the bands were excised from the gel and TA cloned using the pGEM- T easy-vector II cloning kit (Promega). The standard mini-prep step was avoided by using colony PCR ...
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bioRxiv - Microbiology 2023Quote: ... Gene expression was assessed by qPCR using primers listed in the supplemental material Table S6 and the GO Taq PCR kit (Promega). Gene expression was quantified using SYBR Green (Roche ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Quality was assessed with an Agilent Bioanalyser and the quantity was calculated by an RNA-specific kit from Quantus (Promega).
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bioRxiv - Plant Biology 2023Quote: ... Transcription factors were expressed using at least 2000 ng PCR product per sample with the TnT T7 Quick for PCR DNA in vitro protein expression kit (Promega). All reaction volumes were doubled to yield a total of 100 µL protein product per transcription factor ...
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bioRxiv - Microbiology 2023Quote: High-molecular weight genomic DNA was isolated based on a modified version of Promega’s Genomic DNA Isolation Kit (Promega, A1120) [63] ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Bacterial genomic DNA was extracted from static overnight cultures of WS clones using the Wizard Genomic DNA purification kit (A1125, Promega) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: Stereocaulon virgatum RNA was extracted from the fungal culture using the Maxwell® RSC Plant RNA Kit (Promega, Madison, WI). Tissue was homogenized in 30 second intervals for 1.5 minutes at 30hz ...
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bioRxiv - Microbiology 2024Quote: ... Total DNA from feather pulps and tracheas were extracted using the MagaZorb® DNA mini-prep kit (Promega, Madison, WI), following the manufacturer’s recommendations with a few modiications ...
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bioRxiv - Microbiology 2024Quote: ... The bacteria were pelleted by centrifugation and DNA extraction was performed with the Wizard® genomic DNA purification kit (Promega).
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bioRxiv - Developmental Biology 2024Quote: ... The 4 µg of linearized vectors were transcribed 5h using RiboMAX™ Large Scale T7 RNA polymerase kit (Promega, #P1300) with 5.6 mM Ribo m7G Cap Analog (Promega ...
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bioRxiv - Microbiology 2024Quote: ... In vitro mRNA synthesis was then carried out at 37 °C for 4 h in T7 RiboMAX Large Scale RNA Production System kit (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and used the GoScript Reverse Transcription System kit to generate cDNA from extracted RNA (Catalog #A5000, Promega Corporation, Madison, WI). We designed primers using the tool primer-Blast134 and the predicted cDNA sequence of kitlga (Fig ...
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bioRxiv - Plant Biology 2024Quote: One microgram of DNA-free total RNA was reverse transcribed using Avian Myeloblastosis Virus (AMV) reverse transcriptase and oligo(dT)_15 primers (Reverse Transcription System Kit, Promega). PCR amplification was performed with Phusion DNA Polymerase (LifeTechnologies ...
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bioRxiv - Microbiology 2024Quote: A specific double-stranded RNA (dsRNA) was synthesized using the T7 RiboMAX Express RNAi System kit (Promega, Madison, WI, USA), and the primer sequence was listed in Table S4 ...
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bioRxiv - Microbiology 2024Quote: ... Cytotoxicity was measured by quantifying the activity of lactate dehydrogenase (LDH) in the extracellular medium (Cytotox 96 non-radioactive cytotoxicity kit, Promega) and normalised against cells lysed by 0.04% Triton X-100 ...
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bioRxiv - Immunology 2024Quote: ... Supernatants were then assayed for IL-1β (human; DuoSet; R&D) and relative LDH levels (as a surrogate for pyroptosis) using a CytoTox 96 Kit (Promega). For the THP-1 LPS signaling assays ...
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bioRxiv - Immunology 2024Quote: ... Supernatants were then transferred to a 96-well plate for storage and assayed for IL-1β (human; DuoSet; R&D) and relative LDH levels using a CytoTox 96 Kit (Promega). Once supernatants were removed ...
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bioRxiv - Microbiology 2024Quote: ... using random primers for cDNA synthesis and gene-specific primers (primer sequences are listed in S3 table) for qPCR (GoTaq qPCR kit, Promega). The transcript levels for innate immune factors were normalized to the geometric mean of those for the housekeeping genes ACTB and HPRT (ΔCT ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from different tissues of shrimp using the Eastep Super Total RNA Extraction Kit (Promega, Shanghai, China). The genomic DNA of shrimp tissues was extracted using a genomic DNA extraction kit (Vazyme ...
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bioRxiv - Microbiology 2024Quote: ... genomic DNA was genotyped for 15 autosomal STR loci and amelogenin (X/Y) with the Powerplex 16HS PCR kit (Promega). Positive control (gDNA 2800M ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was isolated from the cell lysis using Maxwell® RSC simply RNA Cells/Tissue Kit (Promega, Madison, WI, USA) and cDNA was synthesized ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was extracted from bacterial cells grown overnight in MOKA-rich medium using the Wizard genomic DNA purification kit (Promega) or as described (62 ...