Labshake search
Citations for Promega :
8151 - 8200 of 8279 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: Total RNA was isolated from 1×106 cryopreserved lymph node (LN) mononuclear cells (LNMC) isolated from LN biopsies using the SimplyRNA Tissue kit for the Maxwell RSC instrument (Promega, Madison, WI). RNA was then tested for both SIV gag and a cellular gene ...
-
bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted from Tetrahymena WT (SB210, with or without BrdU-labeling) and ΔAMT1 cells using Wizard® Genomic DNA Purification Kit (Promega, A1120), sheared to 3-5kb in length with Megaruptor (Diagenode Diagnostics) ...
-
bioRxiv - Microbiology 2022Quote: The extent of ADCC activation induced by human γ-globulins was evaluated with the use of an ADCC Reporter Bioassay (Core Kit; Promega, G7010) and the EnSpireMultimode Plate Reader (PerkinElmer) ...
-
bioRxiv - Bioengineering 2023Quote: ... We then performed first-strand cDNA synthesis from 400 ng total RNA in 20 uL reactions using Promega GoScript Reverse Transcription Kit (Promega, catalog # A5000).
-
bioRxiv - Molecular Biology 2023Quote: ... DNA for PCR was isolated from 50 µl of tissue lysate using the Maxwell RSC tissue DNA Kit (Promega, Madison, WI, USA).
-
bioRxiv - Cancer Biology 2023Quote: Total RNA from MB cells treated with RA for 48 h was extracted using the SV Total RNA Isolation System kit (Promega, Madison, USA), following the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2023Quote: ... Single transformed ampicillin resistant colonies were grown in LB + Ampicillin and their DNA was extracted by the Wizard® Plus SV Minipreps DNA Purification System kit (Promega, A1460). Clones with correct insertions were evaluated by Sanger sequencing (LightRun tube ...
-
bioRxiv - Immunology 2023Quote: ... the infectivity of the pseudotyped viruses was determined by measuring the luciferase activities using a PicaGene Luminescence Kit (Fujifilm Wako, Osaka, Japan) with a GloMax Navigator Microplate Luminometer (Promega, WI, USA).
-
bioRxiv - Genomics 2023Quote: Complete genomic DNA was extracted from fresh overnight growth on Nutrient agar (Oxoid, Hampshire, England, U.K) using a Wizard genomic DNA purification kit (Promega Corporation, Madison, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: DNA in the HHMX-stimulated supernatants of THP1 dual reporter cells was quantified using the QuantiFluor dsDNA Detection Kit from Promega (catalog # E2670) according to the manufacturer’s instructions.
-
bioRxiv - Immunology 2023Quote: RNA isolation from the cultured THP1 dual reporter cells was performed by using ReliaPrep RNA Cell Miniprep Kit (Promega, USA, catalog # Z6011) according to the instructions of the manufacturer ...
-
bioRxiv - Microbiology 2023Quote: We isolated the genomic DNA of the strains used in this work using the Wizard genomic DNA purification kit (Promega, WI, USA) and quantified using the Qubit 4 fluorometer (ThermoFisher Scientific ...
-
bioRxiv - Pathology 2024Quote: ... amniotic fluid and eluates from swab samples) using the Viral Total Nucleic Acid Purification Kit for the Maxwell RSC instrument (Promega, Madison WI) with the following modifications ...
-
bioRxiv - Microbiology 2024Quote: ... The PCR products were purified with the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) and quantified using Quantus™ Fluorometer (Promega, USA). Purified amplicons were pooled in equimolar amounts ...
-
bioRxiv - Biochemistry 2024Quote: Total RNA was isolated from the cells of 6-well plates treated as above using the Maxwell RSC simplyRNA kit and the Maxwell RSC Instrument (both Promega, Southampton, UK) according to manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2024Quote: ... Two hundred ng RNA were used to prepare complementary DNA (cDNA) using M-MLV reverse transcriptase kit (Promega, Charbonnières-les-Bains, France) and oligo(dT ...
-
bioRxiv - Neuroscience 2024Quote: ... Total RNA from forebrain of P5 WT (n=2) and TgRBFOX1 (n=2) mice was isolated using the Maxwell® 16 RSC simplyRNA Tissue Kit (Promega, AS1340). Total RNA was quantified by Qubit® RNA BR Assay kit (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2024Quote: ... The resulting PCR products were purified using the GENECLEAN Turbo kit and subjected to in vitro coupled transcription/translation reactions using the TNT® Quick Coupled Transcription/Translation System (Promega, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... Total RNA was extracted using the Maxwell 16 LEV simply RNA tissue kit in combination with a Maxwell nucleic acid extraction robot (Promega, WI, USA) for rabbit caecal content ...
-
bioRxiv - Synthetic Biology 2024Quote: ... cultures were centrifuged and the cell pellets were subjected to genomic DNA isolation using the Wizard Genomic DNA Purification Kit (Promega, Cat. # A1120). Isolated genomic DNA was fully sequenced using Nanopore sequencing (Plasmidsaurus Inc) ...
-
bioRxiv - Pathology 2024Quote: ... Total RNAs were extracted from the leaf sheaths or rice seeding leaves using an Eastep Super Total RNA Extraction Kit (Cat. no. LS1040, Promega, Shanghai, China). Reverse transcription was used to generate cDNA using a reverse transcription kit (Cat ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2 ul were used as templates for PCR with the pairs of primers designed to amplify the genomic region of interest (Table S4) and using GoTaq® Flexi DNA Polymerase kit (Promega, M7806). PCR products were run on agarose (Invitrogen ...
-
bioRxiv - Plant Biology 2024Quote: ... Products were run out on a 1% agarose gel and bands of 1200-1400 bp were excised and subsequently gel extracted with the Wizard SV Gel Cleanup Kit (Promega, https://www.promega.com). Following gel extraction ...
-
bioRxiv - Immunology 2024Quote: ... the first strand cDNA synthesis was conducted using a Reverse Transcription System kit according to the instructions of the manufacturer (Promega, Madison, WI).
-
bioRxiv - Physiology 2024Quote: ... HiBiT intensity was measured daily in three biological replicates (3 measurements x 3 wells x 3 replicates / day) in 384-well format using a HiBiT quantification kit (Promega Nano-Glo). 5µl HiBiT reaction mixture (1:100 LgBiT protein and 1:50 HiBiT lytic substrate in HiBiT lytic buffer ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: RNA was reverse transcribed to complementary DNA (cDNA) using the GoScriptTM Reverse Transcriptase Random Primers kit (Promega Ref A2801, Madison, WI, USA) according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2024Quote: High molecular weight genomic DNA of purified isolates was extracted using the Promega Wizard Genomic DNA Purification Kit (Promega, Madison, WI, USA) and subjected to random amplified polymorphic DNA (RAPD ...
-
bioRxiv - Microbiology 2024Quote: ... DNA was extracted from 150 µL of supernatant using the Maxwell 16 robotic system and corresponding Tissue DNA kit (Promega, WI, USA) following the manufacturer’s instructions.
-
bioRxiv - Genomics 2024Quote: ... Six of these kits were specifically designed for the extraction of high molecular weight (HMW) DNA: the Promega Wizard® HMW DNA Extraction kit (hereafter referred as Promega, P) which uses centrifugation for the DNA cleaning step ...
-
bioRxiv - Molecular Biology 2024Quote: 1mg of total RNA was used as input for the first step of poly(A) mRNA enrichment with the PolyATtract® mRNA Isolation Systems kit (Promega, Z5310) and purified according to the manufacturer’s protocol ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... we apply a standard salt-based method for extracting DNA using the Promega WizardTM Genomic DNA Purification kit (Promega, Madison, WI, USA), followed by library preparation using the NEBNext UltraTM II FS DNA Library Preparation Kit for Illumina (New England Biolabs ...
-
bioRxiv - Cell Biology 2024Quote: ... the PCR product was pooled and the 357 bp product was gel purified with the wizard gel and PCR clean up kit (Promega, Madison, Wisconsin) for sequencing with the Illumina NextseqTM 500 system high-output kit with 75 bp read length ...
-
bioRxiv - Cancer Biology 2021Quote: Genomic DNA from cell lines was isolated using Maxwell® 16 Instrument and the Maxwell® 16 DNA Purification Kit (Promega Cat.# AS1020) following the protocol provided ...
-
bioRxiv - Microbiology 2020Quote: ... for 20 min at 56°C before DNA was purified with Maxwell® RSC Blood DNA Kit (Promega Pte. Ltd., Cat. No. AS1400). DNA concentration was quantified using Qubit® 2.0 fluorometer ...
-
bioRxiv - Microbiology 2020Quote: ... of luciferase was determined using a PicaGene Luminescence Kit (TOYO B-Net Co., LTD, Tokyo, Japan) and GloMax Navigator System G2000 (Promega Corporation, Madison, WI), according to the manufacturer’s protocol.
-
bioRxiv - Microbiology 2020Quote: ... and DNA-concentration was determined using a QuantiFluor® dsDNA kit (detection limit: 50 pg/mL; sensitivity: 0.01 – 200 ng/µL) and Glomax® –Multi+ system (Promega, Madison, WI) with the blue fluorescence optical kit installed (Ex ...
-
bioRxiv - Microbiology 2020Quote: ... Extraction of total RNA from viral transport media was performed using the Maxwell RSC Whole blood RNA/DNA kit (Promega Corporation, Madison, WI) on the Maxwell RSC 16 automated nucleic acid extractor ...
-
bioRxiv - Microbiology 2020Quote: Faecal DNA was extracted and purified using a combination of repeated bead-beating (method 5) (Costea et al, 2017) and the Maxwell 16 Tissue LEV Total RNA Purification Kit (Promega, Maddison, WI, USA), with STAR (Stool transport and recovery ...
-
bioRxiv - Plant Biology 2020Quote: ... and five early developmental buds (one early developmental sample) per individual using the Maxwell 16 LEV Plant RNA Extraction Kit (Promega Corporation, Madison, WI).
-
bioRxiv - Molecular Biology 2020Quote: ... and then cloned to ensure representative amplification of the parental copies using pGEM-T Easy-cloning Vector Kit (Promega Corp., Madison, WI, USA) and transformed into competent Escherichia coli JM109 at 42 °C ...
-
bioRxiv - Developmental Biology 2020Quote: ... DNAs were purified using the PureYield Midiprep kit and were linearized before in vitro synthesis of anti-sense RNA probes using T7 or Sp6 polymerase (Promega, #P2077 and #P108G), RNAse inhibitor and dig-labeled rNTPs (Roche ...
-
bioRxiv - Developmental Biology 2022Quote: ... cells were detached using AccutaseTM and RNA was extracted using the Promega kit ReliaPrep TM RNA Cell Miniprep System (Promega, US. Catalog# Z6011). RNA was reverse transcribed using Omniscipt TM RT kit (Qiagen ...
-
bioRxiv - Plant Biology 2022Quote: Healthy young leaves from each line were collected and genomic DNA was extracted using a Maxwell RSC Plant DNA Kit (Promega, Madison, WI, USA). The presence or absence of the 5.46-kb fragment containing DAI was confirmed by PCR (primers 1 and 2) ...
-
bioRxiv - Cell Biology 2022Quote: ... was used as a template in PCR reactions and amplifications were performed with the GoTaq®G2 Flexi DNA Polymerase kit (Promega, Southampton, UK). Cycling parameters were one cycle of 95°C for 5 minutes for initial denaturation followed by touch-down cycling for the first 14 cycles ...
-
bioRxiv - Microbiology 2020Quote: ... genomic DNA was isolated from the corresponding BKE (Bacillus Knockout Erythromycin collection - (Koo et al., 2017)) strains using the Wizard® Genomic DNA Purification Kit (Promega, Madison, WI). Of note ...
-
bioRxiv - Cancer Biology 2020Quote: ... Lipofectamine 3000 was used to co-transfect HEK-293T cells with wild-type and mutant luciferase reporter vectors as well as P51-ETS1-NC or P51-ETS1.Relative luciferase activity was expressed as the ratio of firefly luciferase activity to sea kidney luciferase activity using a dual luciferase reporting kit (Promega, Madison, Wisconsin, USA) after 48 h.
-
bioRxiv - Immunology 2020Quote: ... were verified by Sanger sequencing and used as DNA templates in the T7 TNT in vitro transcription/translation kit (Promega, Madison, WI; #L1170) using [35S]-methionine (PerkinElmer ...
-
bioRxiv - Pathology 2020Quote: ... cDNA synthesis from the mRNA was performed using a Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) kit (Promega, Madison, Wisconsin, USA) in a Mastercycler® Gradient (Eppendorf ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: Total RNA was isolated from EpCAM+ and EPCAM- cell fractions using a Maxwell® LEV simply RNA Cells/Tissue kit (Promega, WI, USA) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... dahliae strain JR2 was isolated from tomato roots seven days after root dip inoculation and following five days of in vitro growth in soil extract and potato dextrose broth (PDB) using the Maxwell® 16 LEV Plant RNA Kit (Promega, Madison, USA). Real-time PCR was performed as described previously17 to determine the expression of effector genes relative to VdGAPDH with primer pairs as shown in Extended data Table 6.