Labshake search
Citations for Promega :
8051 - 8100 of 8279 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... Organoids were cultured for another 5 days before proceeding with microscopy image and viability test by CellTiter-Glo 3D kit (Promega: G9681) following manufacturer’s instructions.
-
bioRxiv - Biophysics 2023Quote: ... Subsequently we ran the digested fragment on a 1% TAE agarose gel and the desired ∼14 kbp DNA fragment was isolated from an agarose gel using a gel purification kit (Promega, A9282).
-
bioRxiv - Genomics 2023Quote: ... DNA desulphonation and cleanup steps were immediately performed following the manufacturer instructions of the MethylEdge® Bisulfite Conversion System kit from Promega. After the column cleanup ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were cultured in a 6-well plate and transfected with 10 µg of linearised plasmid expressing the SV40 Large T antigen using the FuGene Transfection Kit (Promega, #E2691) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... prior to firefly and Renilla luciferase activity measurements which were performed in triplicate using the Dual-GLO kit (Promega cat. #E2920). The whole procedure was repeated three times independently for each construct.
-
bioRxiv - Developmental Biology 2023Quote: Notch reporter 4xcsl::H2B-mvenus plasmid(Nowotschin et al., 2013) was injected at 30ng/µl after purification using the PureYield Midiprep kit (Promega, #A2492). For antisense in situ hybridization probes ...
-
bioRxiv - Cancer Biology 2023Quote: ... they were treated for the indicated times and viability was assessed using the CellTiter-Glo® 3D Cell viability kit (Promega), following the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2023Quote: ... MDA-MB231 cells were imaged by SEM and quantified by fluorescence analysis of DNA content with the QuantiFluor dsDNA kit (Promega, US). For detection of stem-like properties of cells in scaffolds ...
-
bioRxiv - Bioengineering 2023Quote: ... Substrate-dependent differences of cell adhesion and growth were determined by fluorescence analysis of DNA content with the QuantiFluor dsDNA kit (Promega, US) after 5 h and 7 d of culture ...
-
bioRxiv - Microbiology 2023Quote: DNA extractions for genomic sequencing were performed using Maxwell™ RSC Cell DNA Purification Kit and automated extraction on the Maxwell RSC 48 Instrument (Promega). The DNA concentration was evaluated using a Qubit dsDNA BR assay kit and Qubit Fluorometer (ThermoFisher) ...
-
bioRxiv - Genomics 2023Quote: ... leaves and fruits were collected and in the field and total RNA was extracted with Maxwell® RSC Plant RNA Kit (Promega). Two pools were generated and used for PacBio Iso-Seq library preparation (Procedure & Checklist – Iso-Seq™ Express Template Preparation for Sequel® and Sequel II Systems ...
-
bioRxiv - Developmental Biology 2023Quote: ... Targeted sequence of each single clone was validated by Sanger sequencing after gDNA isolation through Wizard® Genomic DNA Purification Kit (#A1120, Promega). For Prkaa1 knockout ...
-
bioRxiv - Cancer Biology 2023Quote: ... Two replicate electroporations were each divided into three wells for 48h of culture and readout using the Dual-Glo Luciferase kit (Promega; E2940) in a Bio-Tek Synergy HT Microplate Reader.
-
bioRxiv - Neuroscience 2023Quote: ... equal amount of RNA (2 μg) from each sample was subjected to cDNA synthesis using reverse transcriptase Kit (Promega Corporation, USA) following standard procedures ...
-
bioRxiv - Biophysics 2023Quote: ... from the access handles by running this on a 1% agarose gel and gel purify the right DNA fragment with a gel purification kit (Promega, A9282).
-
bioRxiv - Genomics 2023Quote: ... Resulting genomic DNA was assessed for concentration and purity using Promega DNA quantification kit on the Promega Quantus Fluorometer (Promega, Germany) and measurement of A260/A280 ratio on the MultiSkan Sky High spectrophotometer (Thermo Fisher ...
-
bioRxiv - Microbiology 2023Quote: ... Nucleic Acid Purification with Proteinase K digestion was performed using Maxwell ® RSC Total Viral Nucleic Acid Purification Kit according to the manufacturer’s protocols (Promega, USA). For preparation of bacterial genomic DNA ...
-
bioRxiv - Cancer Biology 2023Quote: Genomic DNA was prepared from single cell suspensions of thymic leukemias using the Wizard Genomic DNA Purification Kit (Promega, Madison WI). PCR amplification of the Jak3 pseudokinase domain was performed using MyTaq HS Red Master Mix (FroggaBio ...
-
bioRxiv - Cell Biology 2023Quote: Constitutional genomic DNA is extracted from total bone marrow at the remission and purified using magnetic beads (Maxwell® RSC Whole Blood DNA Kit, PROMEGA). Tumor genomic DNA is extracted and purified using magnetic beads (Maxwell® RSC Tissue DNA Kit ...
-
bioRxiv - Microbiology 2023Quote: ... The amplified PCR fragments were checked for uniformity and size using 0.8% agarose gel electrophoresis and purified using a PCR fragment recovery kit (Promega, WI, USA). The recovered DNA fragments were sequenced using the MiSeq 500PE v.2 kit (Illumina ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Luciferase activity was assayed in an Omega luminescence plate-reader (Fluostar) with four different gains following the instructions of the Nano Dual-Luciferase® Reporter kit (Promega). A further correction for luminescence bleed-through and background signal was applied using the software developed by Mauriet al.58 Then NLuc signal was divided by the FLuc signal to normalize for the transformation efficiency.
-
bioRxiv - Cancer Biology 2024Quote: ... The BSP amplification product was purified by Vazyme Biotech (China) amplification product purification kit and cloned into pGEM-Tasy-vector system (Promega, USA). and the percentages of methylated cytosines over total cytosines were calculated.
-
bioRxiv - Microbiology 2024Quote: ... and tryptone (Becton Dickinson DifcoTM,)) overnight at 35°C and genomic DNA was extracted using the Wizard® Genomic DNA purification kit (Promega) as previously described [35] ...
-
bioRxiv - Microbiology 2024Quote: DNA extraction of the bacterial strains isolated from oysters and cultivated on agar plates was carried with the Wizard® Genomic DNA Purification Kit (Promega) according to the manufacturer instructions ...
-
bioRxiv - Physiology 2024Quote: Total RNA was isolated from snap frozen LV wall or kidney after mechanical grinding (Precellys Evolution, Bertin Technologies, France) using the Maxwell RSC microRNA Tissue kit (Promega, USA). RNA was quantified via NanoDrop (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2024Quote: ... Total RNA was extracted from 10 ⨯ 10 µm FFPE tissue sections using the Maxwell RSC FFPE RNA kit in accordance with the manufacturer’s manual (Promega, United States). RNA concentration was determined using a Qubit 3.0 Fluorometer using the Qubit RNA BR Assay Kit (Thermo Fischer Scientific ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Larvae from progenies with low homing rates of dsxFGFP-null were pooled to perform genomic DNA extraction (Wizard® Genomic DNA Purification Kit, Promega) and subsequently do amplicon sequencing on the dsx target to check for the presence of mutations that could have prevented the homing ...
-
bioRxiv - Microbiology 2024Quote: ... PCR amplified plasmids and DNA fragments were then purified by the Wizard® SV Gel and PCR Clean-Up kit (Promega) followed by DpnI digestion of purified plasmids ...
-
bioRxiv - Microbiology 2024Quote: The high molecular weight genomic DNA of APEC isolates was extracted and purified using a DNA Wizard® genomic purification kit (Promega), according to the instructions from the manufacturer ...
-
bioRxiv - Genetics 2024Quote: ... then genomic DNA was extracted with the QIAamp 96 DNA QIAcube HT Kit and quantified using the QuantiFluor® dsDNA System (Promega). 2-5 ng of DNA from every colony was used as template for targeted amplicon sequencing (Fw primer and Rv primer ...
-
bioRxiv - Plant Biology 2024Quote: RNA was isolated from plant tissues using a Maxwell RSC Instrument and Maxwell RSC Plant RNA Kit (Promega, Madison, WI, USA) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... ATRX 5ʹ-TGAAACTTCATTTTCAACCAAATGCTC-3ʹ and 5ʹ-ATCAAGGGGATGGCAGCAG-3ʹ All PCR reactions were performed using GoTaq® G2 DNA polymerase kit from Promega following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... cell pellets were washed with 1× PBS and resuspended in either RNA lysis buffer (mirVana kit) containing 0.5 U/μL RNAsin Plus (Promega; Madison, WI) for RNA isolation or 1X radioimmunoprecipitation assay (RIPA ...
-
bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from 10 whole fly bodies collected on dry ice between ZT 6 and 8 using SV RNA Total Isolation kit (Promega #Z3101) according to manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2024Quote: ... Each pool was normalized using the ‘Just-a-Plate’ kit (Charm Biotech) then DNA was quantified using the Quantus™ fluorometer (Promega). The final pools were combined in equal molarity and sequenced on the Illumina platform Novaseq 6000 at 250 bp PE.
-
bioRxiv - Cell Biology 2024Quote: The insulin secreted from islets ex vivo and plasma insulin from blood samples a were measured using Lumit Insulin Immunoassay kit (Promega, CS3037A05) as per the manufacturer’s instructions and measured using a Flexstation-3.
-
bioRxiv - Cancer Biology 2024Quote: JMJD6 and YBX1 constructs containing an upstream T7 promoter were linearized and used as a template for coupled transcription and translation in rabbit reticulocyte lysates according to manufacturer’s protocols (TnT kit,Promega Corporation, USA). Synthesis was confirmed using Western blot analysis using respective antibody ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1 µg of total RNA was reversed-transcribed with High-Capacity cDNA Reverse Transcription Kit (Thermo-Fisher # 4368814) and qPCR performed with GoTaq (Promega # M3001) according to instructions with primer sets listed in Table S5 and analyzed likewise 2,−ΔΔCt method (Livak & Schmittgen ...
-
bioRxiv - Developmental Biology 2024Quote: ... Tail clips were taken from each pup on the weaning day and genotyping was performed using the GoTaq® Flexi DNA Polymerase kit (Promega). Separate PCR reactions were set up to amplify either the wildtype or mutant allele using forward wildtype primer (5’-CCCATTGTCAGCGGGCTGTAG-3’) ...
-
bioRxiv - Cell Biology 2024Quote: ... Radiolabeled rat ornithine transcarbamylase (OTC) precursor protein was synthesized from plasmid OTC with the TNT Quick Coupled Transcription/Translation kit (Promega #L2080). Upon import into the mitochondrial matrix ...
-
bioRxiv - Plant Biology 2020Quote: ... the band of interest excised from the gel and cleaned using Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit and cloned into pGEM-T (Promega) for amplification before sequencing.
-
bioRxiv - Cancer Biology 2021Quote: ... genomic DNA was extracted from cells at the indicated time points using the Maxwell® RSC Cultured Cells DNA Kit (Promega AS1620). DNA was amplified using Q5® High-Fidelity 2X Master Mix (New England BioLabs® M0492L ...
-
bioRxiv - Cell Biology 2020Quote: Genomic GNA (gDNA) was extracted from each of the GFP+ and GFP-cell types using the Wizard Genomic DNA Purification Kit (Promega, Southampton, UK) and quantified using a Nanodrop 1000 Spectrophotometer (Thermo Fischer Scientific ...
-
bioRxiv - Microbiology 2021Quote: ... the total genomic DNA from single colonies of isolated bacteria from the custom designed selective medium described in the section 2.3 was conducted using a Wizard™ genomic DNA extraction kit (Promega Corporation, USA), as per the manufacturer instructions ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... DNA extraction was performed as described for whole-genomic DNA purification using the Promega™ Wizard™ Genomic DNA Kit (A1125, Promega). Genome quality and quantity were checked in 1% agarose gels stained with %0.0001 SYBR Safe and measured in a NanoDrop (ACTGene ASP-3700 ...
-
bioRxiv - Immunology 2021Quote: ... vRNA was isolated from plasma samples using the Maxwell Viral Total Nucleic Acid Purification kit on the Maxwell 48RSC instrument (Promega, Madison WI). vRNA was then quantified using a highly sensitive qRT-PCR assay based on the one developed by Cline et al.99 ...
-
bioRxiv - Genomics 2020Quote: Total RNA was extracted using goat tissues from gastrointestinal tract (including abomasum, duodenum, jejunum, ileum, and caecum) using Eastep Super Total RNA Extraction Kit (Promega, Shanghai, China). cDNA was generated via PrimerScriptTM RT Reagent Kit with genomic DNA Eraser (Perfect Real Time ...
-
bioRxiv - Evolutionary Biology 2021Quote: Genomic DNA was extracted from about 20 mg of muscle tissue using the Wizard® Genomic DNA Purification kit (Promega, WI, USA) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: DNA was extracted from concentrated phage samples using a modified protocol with the Wizard DNA Clean-Up Kit (Product #A7280; Promega, Madison, WI). Briefly ...
-
bioRxiv - Neuroscience 2020Quote: The toxicity of PX was investigated on monolayers of BLEC seeded on 96 well plates or on inserts luminal side in co-culture experiments using the commercially available Cytotoxicity Detection Kit (Promega, United States). An aliquot of 25 μl medium was taken to quantify the lactate dehydrogenase (LDH ...