Labshake search
Citations for Promega :
8101 - 8150 of 8279 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... the full-length fragment of human PFKFB3-5 was amplified with primers PFKFB3-5 reverse and PFKFB3-5 forward and subcloned into the plasmid pGEM-T using the T/A Cloning Kit (Promega, Mannheim, Germany). Subsequently ...
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bioRxiv - Cell Biology 2021Quote: ... Cell death was determined according to the activity of lactate dehydrogenase (LDH) in the culture supernatants using a CytoTox® Kit (Promega, USA) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2021Quote: ... total RNA was extracted from ∼25 mg of powdered right brain hemisphere tissue using Maxwell 16 Tissue LEV Total RNA Purification Kit (AS1220, Promega, Madison, WI). UNC HTSF core performed RNA concentration and quality check using fluorometry (Qubit 2.0 Fluorometer ...
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bioRxiv - Microbiology 2020Quote: Total RNA was extracted from 100 μl culture supernatants or apical washes using Monarch® Total RNA Miniprep Kit (Promega, Cat#: T2010S). For viral copy number quantification droplet digital PCR technology was applied (Bio-Rad Laboratories Inc ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR product was run on 2% agarose gel and the expected bands were eluted using Wizard® SV Gel and PCR cleanup kit (Promega, USA),cloned in pGEM-T easy vector (Promega ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from faecal samples not stored in RNAlater® using the Maxwell 16 LEV Blood DNA kit (AS1290, Promega, UK) and automated DNA extraction system ...
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bioRxiv - Microbiology 2021Quote: ... genomic DNA was extracted from an overnight culture of PS1793 using a Promega Maxwell Cell DNA Purification Kit (Promega Corp., Madison, WI). For short-read sequencing ...
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bioRxiv - Evolutionary Biology 2021Quote: ... plantarum genomic DNA was isolated via lysozyme-based cell lysis (47) followed by purification using the Wizard® Genomic DNA Purification Kit according to the manufacturer’s instructions (Promega, Dübendorf, Switzerland). The genome of the reference strain NZ3400* was sequenced at the Functional Genomics Center Zurich (Zürich ...
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bioRxiv - Microbiology 2021Quote: DNA extraction of samples was performed according to an adapted version of the “DNA purification from human feces using the Maxwell® RSC instrument and the Maxwell® RSC PureFood GMO and Authentication Kit” developed by Promega (Promega Corporation ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted from 50–100 mg of EDTA blood or 20 mg of liver using the SimplyRNA Tissue kit and Maxwell RSC16 instrument (Promega, Wisconsin, USA), as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... High quality whole genome was extracted from a pure culture of 16h growth in Luria-Bertani broth (Oxoid, Basingstoke, UK) by using the Wizard Genomic DNA Purification Kit (Promega, Madison, USA) according to the instructions of the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... Cell death of infected cardiomyocytes was determined according to the activity of lactate dehydrogenase (LDH) in the culture supernatants using a CytoTox® Kit (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... PAO1-WT and few colonies that grew in 3 mM 22D-chelated condition were isolated and gDNA extracted using Wizard® Genomic DNA Purification Kit (Promega, USA) for use in whole genome sequencing ...
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bioRxiv - Neuroscience 2022Quote: ... The firefly and Renilla luciferase activity of the cells was quantified by a Dual Luciferase Reporter System Kit (E1910, Promega, WI, USA) following the manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: ... All growth was collected using sterile plastic loops and placed directly into Wizard Genomic DNA purification kit Nuclei Lysis solution (Promega, reference A1120). The Wizard kit extraction protocol was then followed ...
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bioRxiv - Microbiology 2020Quote: ... Filters were transferred to 2 mL microfuge tubes for further processing using the Maxwell® RSC PureFood GMO and Authentication Kit (Promega Corporation) with a modified version of the kit protocol ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was extracted from 1.5□mL of that culture by using a Wizard genomic DNA purification kit (Promega, Madison, WI, USA), prepped using the Nextera XT kit ...
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bioRxiv - Microbiology 2021Quote: ... PA) and AccuMap™ low pH protein digestion kit (with trypsin and lysC) and chymotrypsin (sequencing grade) were from Promega (Madison, WI). PreScisson protease was from GenScript (Piscataway ...
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bioRxiv - Neuroscience 2021Quote: ... Cytotoxicity was determined according to the activity of lactate dehydrogenase (LDH) in the culture supernatants using a CytoTox® Kit (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Positive clones were cultured in liquid LB medium supplemented with kanamycin (50 μg mL−1) for extraction of plasmid DNA with the Wizard Plus SV Minipreps DNA Purification System Kit (Promega - Madison, USA). Plasmids with unique EcoRI/HindIII restriction patterns were subjected to re-transformation and phenotypic confirmation by streaking the clones in solid LB medium supplemented with kanamycin (50 μg mL−1) ...
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bioRxiv - Microbiology 2021Quote: ... baumannii strain AYE (ATCC BAA-1710) was then similarly amplified from genomic DNA prepared with the Wizard Genomic DNA Extraction Kit (Promega, Madison, WI) using primers “F AdeC” and “R AdeC” and cloned downstream from the vector pLac site and Shine-Delgarno sequence in pBMTL-2NTC using vector primers ...
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bioRxiv - Pathology 2022Quote: ... or 300 uL urine+DMSO using the Maxwell RSC Viral Total Nucleic Acid Purification Kit on the Maxwell 48 RSC instrument (Promega, Madison, WI).
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bioRxiv - Cancer Biology 2022Quote: DNA and RNA were isolated using the Maxwell® RSC Cultured Cells DNA and the Maxwell® RSC simplyRNA Cells Kit (Promega) according to manufacturer’s instruction ...
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bioRxiv - Physiology 2022Quote: ... DNA was isolated from snap-frozen ACA tissues with the Maxwell® 16 Tissue DNA Purification Kit (#AS1030, Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted from approximately 106 cells via the Maxwell 16 LEV simply RNA Tissue Kit (Cat. #AS1280, Promega, Madison, WI) on a Maxwell 16 Instrument (Cat ...
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bioRxiv - Genetics 2020Quote: ... Dog DNA was isolated from clinical blood samples provided by CEU Cardenal Herrera Veterinary Hospital of University of Valencia using the Wizard Genome DNA Purification Kit (Promega, WI, USA). The use of dog blood samples was approved by the Animal Experimentation Ethics Committee of CEU Cardenal Herrera University of Valencia ...
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bioRxiv - Genetics 2020Quote: The RNA was isolated using a Maxwell® 16 AS2000 instrument with a Maxwell® 16 LEV simply RNA Tissue Kit (both Promega) following the recommended protocol ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was extracted from whole blood obtained from GALA II study subjects using the Wizard Genomic DNA Purification kits (Promega, Fitchburg, WI), and DNA was quantified by fluorescent assay ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from this concentrated virus sample using a modified protocol with the Wizard Genomic DNA Purification Kit A1120 (Promega, Madison, WI). Briefly ...
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bioRxiv - Cancer Biology 2020Quote: In vitro translation of full-length SREBP1a and full-length SREBP1c was carried out using the TNT rabbit reticulocyte lysate translation kit (Promega, Wisconsin, USA) as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was isolated from tomato freeze-dried tissue (seedlings, leaves or fruit pericarp) using the Maxwell® RSC Plant RNA Kit with the Maxwell® RSC Instruments (Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from these bacterial suspensions using a Maxwell® 16 automated nucleic acid purification system and the Maxwell® tissue DNA purification kit following the manufacturer’s instructions (Promega, UK). Purified DNA extracts were treated with RNase (New England BioLabs ...
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bioRxiv - Genomics 2021Quote: ... germinated urediniospores and from rust infected leaves at 6 and 9 days post inoculation (dpi) was extracted using the Promega Maxwell® RSC Plant RNA Kit with a Maxwell® RSC instrument (Promega.com.au). The spores were induced for germination by placing on the surface of milli-Q water ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Evolved strains were thawed and grown overnight in 150 ml of liquid medium and DNA was extracted using the Wizard Genomic DNA Purification Kit from Promega (WI, USA). One DNA sample (20 to 60 µg ...
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bioRxiv - Evolutionary Biology 2021Quote: Viral RNA (vRNA) was extracted from 100 μl of VTM using the Viral Total Nucleic Acid Purification kit (Promega, Madison, WI, USA) on a Maxwell RSC 48 instrument and eluted in 50 μL of nuclease-free H2O.
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bioRxiv - Microbiology 2021Quote: ... Primary human airway epithelial cells were homogenized in homogenization solution supplemented with 1-thioglycerol and lysed by addition of lysis buffer (Maxwell 16 LEV simplyRNA cells kit; Promega #AS 1270). Prior to RNA extraction ...
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bioRxiv - Microbiology 2020Quote: ... the Wizard Genomic DNA Purification Kit was used including both lysozyme and lysostaphin (10 mg/ml each) (Ref. A1120, Promega Corporation, Spain); PacBio sequencing ...
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bioRxiv - Microbiology 2020Quote: ... All PCR products were purified with an AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, USA) and quantified using a QuantiFluor™-ST (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: The extent of ADCC activation induced by human γ-globulins was evaluated with the use of an ADCC Reporter Bioassay (Core Kit; Promega, G7010) and the EnSpireMultimode Plate Reader (PerkinElmer) ...
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bioRxiv - Immunology 2023Quote: Total RNA was isolated from 1×106 cryopreserved lymph node (LN) mononuclear cells (LNMC) isolated from LN biopsies using the SimplyRNA Tissue kit for the Maxwell RSC instrument (Promega, Madison, WI). RNA was then tested for both SIV gag and a cellular gene ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted from Tetrahymena WT (SB210, with or without BrdU-labeling) and ΔAMT1 cells using Wizard® Genomic DNA Purification Kit (Promega, A1120), sheared to 3-5kb in length with Megaruptor (Diagenode Diagnostics) ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA from MB cells treated with RA for 48 h was extracted using the SV Total RNA Isolation System kit (Promega, Madison, USA), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was extracted at the NCPHL from subcultures inoculated with single bacterial colonies and grown in nutrient agar (Oxoid, USA) at 37°C overnight according to the manufacturer instructions (Wizard® Genomic DNA Purification kit, Promega, UK). Genomic DNA samples (derived from 10 environmental and 250 clinical samples ...
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bioRxiv - Physiology 2024Quote: RNA was isolated from islets from ∼15-week-old male mice (WT and Kcnk16 L114P (L/P)) using Maxwell® 16 LEV simplyRNA Purification Kits (Catalog no. AS1280, Promega, USA). RNA integrity was analyzed using an Agilent 2100 Bioanalyzer ...
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bioRxiv - Molecular Biology 2024Quote: ... tissue homogenization was carried out by bead beating and immediately followed by RNA extraction using the Maxwell® RSC simplyRNA Tissue Kit (Promega AS1340) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: Complete genomic DNA was extracted from fresh overnight growth on Nutrient agar (Oxoid, Hampshire, England, U.K) using a Wizard genomic DNA purification kit (Promega Corporation, Madison, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... and the three randomly selected variants (AMA22_1 IHCA and AMA23 IHC AVI 4 μg/mL and R AVI 8 μg/mL) was performed following the manufacturer’s instructions using the Wizard Promega kit (Promega, Madison, WI, USA). For whole genome sequencing ...
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bioRxiv - Immunology 2023Quote: DNA in the HHMX-stimulated supernatants of THP1 dual reporter cells was quantified using the QuantiFluor dsDNA Detection Kit from Promega (catalog # E2670) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: RNA isolation from the cultured THP1 dual reporter cells was performed by using ReliaPrep RNA Cell Miniprep Kit (Promega, USA, catalog # Z6011) according to the instructions of the manufacturer ...
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bioRxiv - Developmental Biology 2023Quote: ... Single transformed ampicillin resistant colonies were grown in LB + Ampicillin and their DNA was extracted by the Wizard® Plus SV Minipreps DNA Purification System kit (Promega, A1460). Clones with correct insertions were evaluated by Sanger sequencing (LightRun tube ...