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Citations for Promega :
7201 - 7250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... on the Envision plate reader after cell lysis with 1X passive lysis buffer (E1941, Promega). Results were normalized to the total protein amount per well determined using a BCA protein assay kit (Pierce) ...
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bioRxiv - Molecular Biology 2023Quote: ... after cell lysis with 1X passive lysis buffer (E1941, Promega), on the Envision (Perkin Elmer plate reader) ...
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bioRxiv - Molecular Biology 2023Quote: ... a second 96-well plate was infected with P1 to P4 stocks according to the end-point titration method and NLuc was measured with the Nano-Glo Luciferase Assay System (Promega) after cell lysis with 1X passive lysis buffer (E1941 ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 μL 5 ng/μL sequencing grade trypsin (Promega; V5111) prepared in 50 mM ABC supplemented with 1 mM CaCl2 were added to the gel pieces and incubated 30 min at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were directly digested by incubation with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) overnight at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: Assays used the Dual-Luciferase Reporter Assay System (Promega, catalog # E1910). Each transfected well was lysed into 200μl Passive Lysis Buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... and 36 µL of Fugene (Promega #E2691) were mixed and incubated at room temperature for 15 min before being added to a 10-cm plate containing 5 million HEK-293T cells ...
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bioRxiv - Genomics 2023Quote: ... pGL3- Promoter (Promega, E1761), and pGL3-basic (Promega ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed in 1×PBST and then incubated with the secondary antibody (anti-rabbit or anti-mouse IgG conjugated with horse radish peroxidase at 1:10 000, Promega). The membranes were then revealed using an ECL kit (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... The bacterial cells were collected from 2mL of the culture and the genomic DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega). Samples were then sent to Novogene Co. ...
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bioRxiv - Genomics 2023Quote: ... Relative luminescence signals were measured using the Dual-Luciferase Reporter Assay System (Promega, E1960) and GloMax® 20/20 Luminometer by normalizing firefly luciferase signal with renilla luciferase signal.
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bioRxiv - Genomics 2023Quote: ... including the pRL-TK Renilla luciferase control vector (Promega, E2241), pGL3- Promoter (Promega ...
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bioRxiv - Physiology 2023Quote: ... for 45 minutes as per the protocol described elsewhere(Villanueva et al, 2020) These samples were then subjected to trypsin (cat no V5111 sequencing grade, Promega) digestion in an enzyme to substrate ratio of 1:20 (trypsin ...
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bioRxiv - Bioengineering 2023Quote: ... this template was generated from the cDNA of SARS-CoV-2 AI/I-004/2020 using the T7 RiboMAX Express Large Scale RNA Production System (Promega, Madison, WI, USA). The primers and probe used to detect the WK-521 strain were as follows ...
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bioRxiv - Bioengineering 2023Quote: ... imaging was performed 10 min after intraperitoneal (i.p.) injection with 200 μL of 15 mg/mL luciferin substrate (Promega). The total flux of luminescence was calculated by gating a region of interest (ROI ...
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bioRxiv - Cancer Biology 2023Quote: ... were authenticated by the Genetic Resources Core Facility at the John Hopkins University School of Medicine by performing short tandem repeat profiling via GenePrint 10 (Promega) and comparing to the ATCC database ...
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bioRxiv - Genomics 2023Quote: ... 1 μL of each primer (10 μM) and 12.5 μL of GoTaq Green Master mix (Promega®, USA). The PCR was run in a thermocycler (BioRad ...
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bioRxiv - Immunology 2023Quote: ... and luciferase activity (RLU) was measured using the ONE-GloTM Luciferase Assay System (Promega) and Clariostar Plus Microplate Reader (BMG Labtech).
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bioRxiv - Microbiology 2023Quote: ... dissolved in 2 volumes of 0.1 M Tris-HCl (pH 8.0) with the addition of 1 μg of trypsin (Sequencing Grade Modified, Promega) and incubated for 12 hours at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were digested with trypsin (Promega; 1:100 (w/w) enzyme-to-protein ratio ...
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bioRxiv - Plant Biology 2023Quote: ... 10 μg/mL Trypsin (Promega) and 1 mM DTT] was added to digest protein samples on beads ...
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bioRxiv - Bioengineering 2023Quote: HaloTagged AP-2 was visualized in the SUM159 cells through the addition of membrane-permeable JaneliaFluor646-HaloTag ligand (Promega) at a concentration of 125 nM ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were transfected the next day with a mixture of TP1-firefly luciferase and pRL-TK (Promega) plasmids at a 49:1 ratio using Lipofectamine 2000 (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were then incubated overnight at 37°C with 100 ng trypsin (sequence grade, Promega). The peptides formed from the digestion were further purified by µC18 ZipTips (Millipore ...
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bioRxiv - Cell Biology 2023Quote: The ApoTox-Glo triplex assay (Promega, G6320) were performed in 96 well plates (Greiner Bio-one #655090 ...
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bioRxiv - Cancer Biology 2023Quote: The NAD/NADH-Glo Assay Kit (Promega) was used to measure NAD+ abundance in HCT 116 nuclear extracts ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was determined after 72 h using the CellTiter-Glo® luminescent assay (Promega, catalog number G9242). Cell viability of DMSO-treated cells was set to 100% ...
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bioRxiv - Molecular Biology 2023Quote: ... Cytopathic effect was assessed using the Viral ToxGlo Assay kit (Promega) 72 h after infection ...
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bioRxiv - Biophysics 2023Quote: ... Apoptosis and necrosis assays were performed with a RealTime-Glo Annexin V Apoptosis and Necrosis Assay kit (JA1011, Promega, Madison, WI, USA) at the indicated times after changing the culture medium ...
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bioRxiv - Biophysics 2023Quote: ... The reference vector pRL-TK Renilla plasmid was purchased from Promega UK Ltd and was co-transfected with each of the Firefly plasmids using Lipofectamine 2000 (Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... or mutant β2AR INTAL and a plasmid encoding a cyclic-permuted luciferase reporter construct (pGloSensor-20F, Promega) and luminescence values were measured ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested in 100 mM ammonium bicarbonate containing 2 μg of trypsin/Lys-C enzyme mix (Promega) at 37 °C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... the efficiency of capture on streptavidin-coated paramagnetic beads (Promega) was evaluated as described before ...
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bioRxiv - Bioengineering 2023Quote: ... coli (Promega, JM109), and plated on LB-Ampicillin plates ...
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bioRxiv - Bioengineering 2023Quote: ... TMLCs were then lysed and the luminescence was measured using a luciferase assay kit (Promega) and luminometer (Centro LB ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were then in-gel digested using modified trypsin (sequencing purity, Promega), as previously described (Casabona et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... NAD+ levels were then measured using a GloMax microplate reader (Promega) and following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... using SIRT-Glo Assay kit (Promega), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... were made by replacing the ORF3 region encoding 5–105 amino acids with an in-frame insertion downstream of 4th VP2 codon sequence of UnaG and NanoLuc (Promega) coding regions with their own stop codons ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant plasmids were confirmed by PCR (GoTaq DNA Polymerase, Promega) and Sanger sequencing (Azenta) ...
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bioRxiv - Molecular Biology 2023Quote: ... Both DNA and RNA luminescence assays were performed using the Dual-Glo Luciferase assay system (Promega) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Surface expression was measured using a HiBit-tagged 5-HT2A receptor and the Nano-Glo HiBit Extracellular Detection System (Promega). N-terminal HiBit-tagged human 5-HT2A receptor was cloned into pcDNA3.1 using Gibson Assembly ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5μl of GTP (100mM, Promega®), 1μl of Ribolock™ (40U/μl ...
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bioRxiv - Cell Biology 2023Quote: ... a master mix was prepared by diluting the Extracellular NanoLuc Inhibitor at a 1:1000 ratio and the NanoBRET Nano-Glo Substrate at a 1:333 ratio in PBS (Promega, Madison, WI, USA). Aliquots of the Nluc substrate/inhibitor master mix (100 µl ...
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bioRxiv - Cell Biology 2023Quote: ... the indicated samples were subjected to RNAse ONETM (Promega®) using 10U of the enzyme in the presence of urea (300mM final concentration ...
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bioRxiv - Microbiology 2023Quote: ... 1 μL RNasin Plus RNase inhibitor (40 U/μL, Promega) and 6.5 μL saturated trehalose ...
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bioRxiv - Microbiology 2023Quote: ... GoTaq green polymerase (Promega), and DMSO (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... a complete standard mixture of amino acids (Promega, Medison, WI, USA) was analyzed by CE-MS to obtain MS and MS/MS spectra of the proteinogenic amino acids ...
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bioRxiv - Cancer Biology 2023Quote: Extracellular lactate was measured by Lactate-Glo assay (Promega, J5021). MM134 were reverse transfected with siRNA as above in 96-well plates (25k cells/well) ...
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bioRxiv - Cell Biology 2023Quote: ... primers designed for the gene of interest (Table 1) and GoTaq(R) qPCR Master Mix (Promega). Samples were run on an AB 7300 Real Time PCR System (Applied Biosystems ...