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Citations for Promega :
601 - 650 of 1197 citations for Transcription Factor 15 TCF15 Antibody FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Reverse transcription was performed with Moloney murine leukemia virus reverse transcriptase (MMLV-RT) according to the manufacturer’s protocol (Promega) using 200 ng of total RNA ...
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bioRxiv - Genetics 2020Quote: ... Protein extracts were obtained from in vitro coupled transcription/translation systems (TNT® T7 Coupled Reticulocyte Lysate Systems, Promega). 15 µl of Flag-tagged proteins from TNT assays were pre-incubated on ice for 10 minutes in modified DBB (DBB ...
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bioRxiv - Cell Biology 2022Quote: ... and complementary DNA synthesis was performed using M-MLV reverse transcription in the presence of RNasin RNase inhibitor (Promega). PCR primers were all intron spanning ...
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bioRxiv - Microbiology 2022Quote: Two micrograms of DNAse-free RNA was used for cDNA synthesis using an InProm-II Reverse transcription system (Promega) according to the manufacturer’s recommendation ...
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bioRxiv - Molecular Biology 2022Quote: Complementary DNA was prepared from total RNA extracted from indicated samples using the ImProm-II Reverse Transcription System (Promega). qRT-PCR analysis was performed using a master mix with ROX reference dye (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... RNA reverse transcription and cDNA amplification was then carried out by the GoTaq 1-Step RT-qPCR System (Promega), using established primers (31) ...
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bioRxiv - Microbiology 2023Quote: ... In vitro transcription was performed using T7 RiboMAX Express Large Scale RNA Production System following manufacturer’s protocol (Promega, WI). RNA was quantitated by nanodrop on DS-11 FX Spectrophotometer (DeNovix ...
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bioRxiv - Microbiology 2023Quote: ... 2 μg of RNA was used for cDNA synthesis by Go Script TM Reverse Transcription System (Promega, Madison, USA). Then qRT-PCR was performed using Maxima SYBR Green/ROX qPCR master mix (Thermo Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... One microgram of RNA was converted to cDNA using ImProm-II™ Reverse Transcription System (PROMEGA, Madison, WI, USA) following the manufacturer’s instructions performed on T100™ Thermal Cycler (BIO-RAD) ...
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bioRxiv - Bioengineering 2023Quote: ... DNA libraries were used as templates for in vitro transcription by RiboMAX Large-scale RNA production system-T7 (Promega). The reaction mixture was incubated at 37°C for 2 h ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was used for first-strand cDNA synthesis with the Reverse Transcription System I (Promega, Madison, WI, USA), then diluted 1:50 in water ...
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bioRxiv - Molecular Biology 2023Quote: ... 400 ng of total RNA was transcribed to cDNA using the ImProm-II™ reverse transcription kit (Promega, A3500) with random primers ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA (1-2 μg) was reverse transcribed with a GoScriptTM Reverse Transcriptase cDNA reverse transcription kit according to the manufacturer’s instructions (Promega Corporation ...
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bioRxiv - Cell Biology 2023Quote: ... Amplified constructs were used as inputs for reactions (final reaction concentration of 6.96 ng/µL) using the TnT Quick Coupled Transcription/Translation System (Promega). Reactions were performed as described for the TnT system ...
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bioRxiv - Neuroscience 2023Quote: ... The antisense radio-labeled 35S-Vglut2 riboprobe was generated by in vitro transcription using a T7 RNA polymerase (Promega). 35S-labeled riboprobe was diluted in hybridization solution (50% formamide ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA samples (from three independent plants in all cases) were subjected to reverse transcription with the AMV enzyme (Promega) and random hexanucleotides as primers ...
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bioRxiv - Cell Biology 2024Quote: ... Stag2 and Pds5b variants were first expressed by ‘cold’ coupled in vitro transcription- translation (IVTT; TNT reticulocyte lysate, Promega). IVTT aliquots (3 µl ...
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bioRxiv - Immunology 2024Quote: ... RNA was subjected to reverse-transcription using GoScript reverse transcriptase primed with a mix of Oligo(dT)s (Promega) and random hexamers (Promega) ...
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bioRxiv - Molecular Biology 2020Quote: ... lysed by rocking for 15 min at room temperature in 500 μL of Passive Lysis Buffer (Promega), and clarified by centrifugation at 21,000 × g for 2 min ...
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bioRxiv - Biochemistry 2022Quote: ... after which the dsDNA template was degraded for 30 minutes with 15 units of RQ1 DNAse (Promega). The transcribed sgRNA was PAGE purified on an 8% denaturing polyacrylamide gel containing 7 M urea ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated with cell-permeant TMR red Halo-tag ligand (1:2,000 for 15 min, Promega) 24 hours post-transfection ...
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bioRxiv - Molecular Biology 2020Quote: shERH plasmids were diluted in 500 μl of OPTI-MEM with 15 μl of Fugene HD (Promega) and mixed well with rapid pipetting ...
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bioRxiv - Molecular Biology 2019Quote: ... lysed by rocking for 15 min at room temperature in 500 μL of Passive Lysis Buffer (Promega), and clarified by centrifugation at 21,000 x g for 2 min ...
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bioRxiv - Microbiology 2019Quote: ... lysed by rocking for 15 min at room temperature in 300 μl of Passive Lysis Buffer (Promega), and clarified by centrifuging at 14,000 g for 1 min ...
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bioRxiv - Immunology 2021Quote: ... The fluorescent output was measured every 15 min at room temperature on a GloMax Discover (Promega Instrument) microplate reader (Ex= ∼495 nm ...
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bioRxiv - Cancer Biology 2022Quote: ... Plates were shaken for 15 min and bioluminescence was determined using the GloMax Discover microplate reader (Promega). Blank-corrected bioluminescence values were normalized to DMSO-treated wells ...
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bioRxiv - Molecular Biology 2023Quote: ... lysed by rocking for 15 min at room temperature in 500 µl of passive lysis buffer (Promega), and clarified by centrifugation for 2 min at 21,000 x g ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA was retrotranscribed into cDNA using M-MLV reverse transcriptase with Oligo(dT)15 primers (Promega) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... using a dounce homogenizer and incubated for 15 minutes on ice and treated with RQ1 DNase (Promega) for 5 min at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... using a dounce homogenizer and incubated for 15 minutes on ice and treated with RQ1 DNase (Promega) for 5 min at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... 15 mg of His-Tev-HaloTag-(3C)-human γ-TuNA was coupled to Halo Magne beads (Promega). Xenopus laevis egg extract was prepared by standard methods28 ...
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bioRxiv - Microbiology 2023Quote: ... for at least 15 minutes and luciferase activity was measured with Dual-Glo Luciferase Assay System (Promega) following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... proteins were produced from pcDNA3-based plasmids at 30 °C for 90 min using the in vitro transcription & translation (IVTT) kit (Promega), following manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... NPs and Su9-DHFR were in vitro translated using rabbit reticulocyte lysate with either the TnT SP6 quick couple Transcription/Translation System (Promega) for cDNA sequences flanking the SP6 promoter ...
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bioRxiv - Molecular Biology 2022Quote: ... and random hexamers were used for reverse transcription and PCR amplification was carried out using 25 cycles and GoTaq polymerase (Promega) with linker specific primers ...
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bioRxiv - Neuroscience 2021Quote: Radioactive U1 and U7 snRNAs were generated by run-off transcription with T7 polymerase from linearized plasmid DNA templates in the presence of m7G cap analog (Promega) using the MEGAshortscript T7 kit (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... of linearized DNA were used as templates for in vitro transcription in the T7 RiboMAX Large Scale RNA Production System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 μl of Reverse Transcription Mix (10 μl of 10 mM dNTPs and 40 μl of 5X MMLV RT Buffer Promega), 1 μl of 10 mM ddTTP for the sequencing reaction and 1 μl of MMLV Reverse Transcriptase RNAse H minus (Promega ...
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bioRxiv - Developmental Biology 2019Quote: ... Species-specific digoxigenin (DIG) labeled riboprobes were generated by in vitro transcription of Crabp1 fragments cloned in pGEMT-Easy (Promega) vector ...
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bioRxiv - Cell Biology 2019Quote: A DNA fragment of Il24 (−1036 ∼ −598 bp in the upstream of transcription start site) was subcloned into a luciferase reporter vector pGL4 (Promega). AML12 cells were cultured in 24- well plates for 24 hours ...
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bioRxiv - Molecular Biology 2019Quote: ... The first strand cDNA was synthesized with 2 μg of purified total RNA using the reverse transcription system (Promega, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: In vitro translation reactions were performed using coupled bacteriophage T7 RNA polymerase transcription-rabbit reticulocyte lysate translation reactions as per the manufacturer’s instructions (Promega TNT). Samples were radiolabelled with 35S-methionine (Perkin Elmer ...
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bioRxiv - Immunology 2021Quote: ... and cDNA was synthesized via reverse-transcription of 1 μg of RNA using M-MLV Reverse Transcriptase (Promega (Wisconsin, USA). cDNA was used to analyse differences in gene expression by qRT-PCR employing iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Genomics 2021Quote: ... full-length BORIS and CTCF 11 ZF domain were translated in vitro using the TnT Quick Coupled Transcription/Translation System (Promega).
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bioRxiv - Genetics 2019Quote: In vitro transcription and translation were realized from 500ng of cDNA pGEM-T construct using the TnT T7 Quick Coupled Transcription/Translation kit (Promega) and Transcend Biotin-Lysine-tRNA following the manufacturer protocol ...
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bioRxiv - Microbiology 2021Quote: ... proteins were expressed by in vitro transcription/translation using the TNT SP6 High-Yield Wheat Germ Protein Expression System (Promega) prior to incubation with the affinity resins ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 4 μg of the extracted material was then used as template to in vitro synthesized viral genomic RNA transcripts using the Ribomax T7 RNA transcription Kit (Promega) and Ribo m7G Cap Analogue (Promega ...
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bioRxiv - Biochemistry 2020Quote: All basic transcription-translation reactions for PrP constructs were performed using a Rabbit Reticulocyte Lysate (RRL) derived SP6 coupled system (Promega) for 30 min at 30 °C in the presence of 2.5 μCi [35S]-methionine in a total volume of 10 μl ...
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bioRxiv - Bioengineering 2020Quote: ... Reverse transcription into cDNA was done from 3 μg of RNA by using 500 μg/mL random hexamers (Promega, Switzerland) and 0.5 μL of 200 UI/mL SuperScript III reverse transcriptase (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μg of total RNA was used for first strand cDNA synthesis with the ImProm-II Reverse Transcription System (Promega) according to the manufacturer’s instructions ...