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Citations for Promega :
451 - 500 of 1197 citations for Transcription Factor 15 TCF15 Antibody FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: IVTT reactions were performed using the SP6 TnT® Quick Coupled Transcription/Translation System (Promega #L2080) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... Reverse transcription (RT) was carried out using 2.5 U avian myeloblastosis virus (AMV) reverse transcriptase (Promega) and a 32P-labelled primer in the presence of 4 mM MgCl2 and 0.5 mM dNTPs ...
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bioRxiv - Immunology 2019Quote: ... RNA was converted into cDNA using GoScript™ Reverse Transcription System (Product code: A5001, Promega, UK). qPCR for the RSV L gene was performed on a Stratagene Mx 3005p (Agilent technologies ...
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bioRxiv - Genetics 2019Quote: ... Digoxigenin-labeled probes were then synthesized using in vitro transcription (T7 RNA Polymerase, Promega / Life Technologies), ethanol precipitated ...
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bioRxiv - Plant Biology 2019Quote: ... we used a control plasmid provided in the TnT® Quick Coupled Transcription/Translation System (Promega). The plasmid was linearized using the restriction enzyme SacI and purified using the DNA Clean and Concentrator Kit (Zymo Research) ...
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bioRxiv - Genetics 2020Quote: ... from the whole liver and reverse-transcribed into cDNAs using the Reverse Transcription System (Promega, A3500). qRT-PCR was performed using SYBR Premix Ex Taq kit (TaKaRa ...
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bioRxiv - Developmental Biology 2021Quote: Proteins were synthesized with the TnT SP6 Quick Coupled Transcription/Translation System (Promega, Madison, Wisconsin, USA).
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bioRxiv - Molecular Biology 2019Quote: ... using a coupled transcription-translation system containing wheat germ extract (TNT kit; Promega, Cat no. L5030), according to the manufacturer protocol.
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bioRxiv - Molecular Biology 2019Quote: ... The extracted RNA was reverse transcribed into cDNA using GoScript™ Reverse Transcription System (Promega, USA) as per the manufacture’s instruction ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription of 2 μg RNA was performed using M-MLV reverse transcriptase (Promega, WI, USA). The PCR-based amplification was done using synthesized-cDNA ...
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bioRxiv - Bioengineering 2021Quote: ... The 1 μg linearized plasmid was serving as a template for in vitro transcription (SP6, Promega), and the products was then transfected into cells with Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: Plasmids for preparing DNA templates for in vitro transcription were assembled using the pGEM-11Zf(+) (Promega) backbone ...
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bioRxiv - Bioengineering 2021Quote: ... and 250 ng were processed by in vitro transcription using the HiScribe T7 kit (Promega, E2040S) and incubated at 37 °C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was synthesized from one μg RNA with GoScript™ Reverse Transcription System (Promega, Benelux BV) and thereafter diluted to 1:30 (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... In vitro transcription was performed using the T7 RiboMAX Express Large Scale RNA Production System (Promega), followed by Lithium Chloride precipitation to purify the synthesized RNA.
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bioRxiv - Microbiology 2022Quote: In vitro transcription was carried out in T7 RiboMAX Large Scale RNA Production System kit (Promega) according to manufacturer instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... The digoxigenin-labeled antisense cRNA probe was synthesized by in vitro transcription with T7 polymerase (Promega), RNA labeling mix (Roche) ...
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bioRxiv - Immunology 2022Quote: ... Brazil.The GoScript reverse transcription system kit and CytoTox 96 Non-Radioactive Cytotoxicity Assay were from Promega. Brazol reagent was from LGC Biotecnologia ...
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bioRxiv - Microbiology 2022Quote: ... T7 transcription was carried out using T7 RiboMax Express large scale RNA production kit (# P1320, Promega).
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bioRxiv - Molecular Biology 2022Quote: ... were incubated with 35S labeled proteins made by using TNT Quick Coupled transcription/Translation System (Promega) with 35S labeled methionine ...
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bioRxiv - Neuroscience 2023Quote: ... The linearized plasmids were used as templates for in vitro transcription with T3 RNA polymerase (Promega) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2022Quote: Synthesis of S35-labled CaVβ chimeras was done by coupled in vitro transcription-translation (Promega (L1170)) ...
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription was then performed with 50 U of Avian Myeloblastosis Virus reverse transcriptase (Promega Corporation) for 20 min at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was produced using random hexamers as primers and the GoScriptTM Reverse Transcription kit from Promega. Amplification of 16S rRNA and fcrX cDNA was made using SsoFast EvaGreen Supermix 2X kit (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: Complementary DNA (cDNA) was synthesized from RNA using the ImProm-II Reverse Transcription System (Promega, #A3800) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Sly1-HA3 was synthesized with the TNT® SP6 Quick Coupled Transcription/Translation system (#L2080, Promega), according to the instructions of the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: Reverse transcription was performed using the Moloney Murine Leukemia Virus Reverse Transcriptase (Promega, Madison, WI, USA), 1000 ng of total RNA and random hexamers ...
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bioRxiv - Systems Biology 2023Quote: ... RNA was reverse-transcribed into cDNA using ImProm-II™ Reverse Transcription System (Promega, WI, USA). Real-time PCR was conducted using the SYBR green PCR reagent (Roche ...
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bioRxiv - Immunology 2023Quote: In-vitro translation assay was performed using TNT® Quick Coupled Transcription/Translation System (Promega #L1170) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... Complimentary DNA was prepared from 1 µg of RNA using a Reverse Transcription Systems Kit (Promega). Quantitative PCR (qPCR ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Digoxigenin-labeled probes were then synthesized using in vitro transcription (T7 RNA Polymerase, Promega / Life Technologies), ethanol precipitated ...
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bioRxiv - Microbiology 2024Quote: ... in vitro transcription was carried out using the Ribomax large-scale RNA production systems from Promega, and capping using the ScriptCap m7G capping system (Cellscript).
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bioRxiv - Physiology 2024Quote: ... Reverse transcription was performed using oligo(dT) primers and M-MLV Reverse Transcriptase (Promega, Madison, WI) at 42°C for 1 hour according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The cDNA was transcribed from 300 ng total RNA using the reverse transcription system (Promega, USA) in a final volume of 50 μl ...
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bioRxiv - Plant Biology 2024Quote: cDNA synthesis was performed with the GoScript™ Reverse Transcription System A5000 (Promega, Madison, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: Complementary DNA (cDNA) was synthesized from RNA using the ImProm-II Reverse Transcription System (Promega, #A3800) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... the fusion proteins were expressed using the cell-free transcription and translation system (Cat. # L4140, Promega) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... The plates were read within 15 minutes (GloMax Discover luminometer, Promega, Madison, WI, USA) equipped with 450 nM BP filter (donor ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were incubated for 15 min with the HaloTag® TMR G8252 ligand (Promega) in fresh medium ...
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bioRxiv - Cell Biology 2021Quote: ... 9 μl purified RNA was incubated with 1 μl Oligo(dT)15 primers (Promega) at 80°C for 3 min ...
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bioRxiv - Microbiology 2021Quote: ... PFGE of total DNA either undigested or digested 15 min with S1 nuclease (Promega) was carried out as described by Freitas et al ...
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bioRxiv - Biochemistry 2022Quote: ... to 100 µl add 15 µl DNase I and 1.5 µl RNASin Plus (Promega) before use) ...
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Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: ... for 15 minutes at 37°C before lysing with reporter lysis buffer (Promega, E397A). Samples were spun at 15,000 rpm for 10 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 15 ng of pRL-null Renilla luciferase control reporter (#E2271, Promega; for background normalization), and compensated with empty vector control (pCMV2c ...
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bioRxiv - Developmental Biology 2023Quote: Carm1 promoter region (-630 to +15) was cloned to pGL4 luciferase reporter vector (Promega) to be the Carm1-reporter ...
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bioRxiv - Biochemistry 2023Quote: ... peptides were collected as described in ProteaseMax application note (Promega, TB373, Rev. 2/15), and purified using Pierce C18 Spin Tips (Thermo Fisher PI84850) ...
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bioRxiv - Cell Biology 2022Quote: ... were stained with CaspACE FITC-VAD-FMK (fluorescein isothiocyanate-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone) in situ Marker (Promega, Mannheim, Germany) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: The increase in caspase like enzymatic activity was evaluated by using a staining solution containing (FITC)-VAD-FMK (CaspACE™, Promega) at a final concentration of 10 µM/ml (74) ...
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bioRxiv - Biophysics 2021Quote: ... and were produced by translation in rabbit reticulocyte lysates using TnT Quick Coupled Transcription/Translation System (Promega) (see Supplementary Table 5 for plasmids) ...
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bioRxiv - Cancer Biology 2021Quote: ... One μg of RNA was reverse transcribed into single stranded cDNA (Go Script Reverse Transcription System, Promega) and subsequently used for qPCR analyses on a Step One Plus real time PCR detection system (Applied Biosystems) ...