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Citations for Promega :
401 - 450 of 1197 citations for Transcription Factor 15 TCF15 Antibody FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... In vitro transcription and translation experiments were done with rabbit reticulocyte lysate (TNT systems, Promega) according to the manufacturer’s recommendation ...
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bioRxiv - Neuroscience 2019Quote: ... Reverse-transcriptase reactions were performed with the ImProm-II Reverse Transcription System (Promega, Madison, USA) using 2 μg total RNA ...
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bioRxiv - Genomics 2019Quote: ... RNA probes were labeled by in vitro transcription of plasmids with T7 RNA polymerase (Promega) in the presence of Biotin RNA labeling mix (Roche) ...
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bioRxiv - Biochemistry 2020Quote: ... Radiolabelled PDIP38 preprotein was synthesized using TNT® SP6 Quick Coupled Transcription-Translation System (Promega) with undigested pOTB7/PDIP38 as template and 11 μCi of [35S]Met/CysEXPRE35S35S protein labelling mix (specific activity of >1000 Ci/mmol ...
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bioRxiv - Genetics 2021Quote: ... DIG-labelled RNA probes were produced by in vitro transcription with T3-RNA polymerase (Promega). The Crabp1 probe template was amplified from a plasmid containing Crabp1 cDNA sequence ...
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bioRxiv - Cell Biology 2022Quote: Transcription of pGEM plasmids in vitro was carried out according to the manufacturer’s instructions (Promega) using SP6 polymerase ...
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bioRxiv - Developmental Biology 2022Quote: ... one microgram of RNA was reverse transcribed using the ImProm-II Reverse Transcription System (Promega). Quantitative RT-PCR was performed in triplicate with custom-designed oligos using the CFX96 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Genetics 2022Quote: ... In vitro transcription was performed with the T7 RiboMAX™ Kit (Promega, cat. no. P1320). Transcripts were purified by phenol-choloroform extraction and isopropanol precipitation.
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bioRxiv - Neuroscience 2022Quote: ... one microgram of RNA was reverse transcribed using the ImProm-II Reverse Transcription System (Promega), thereafter qRT-PCR was performed in triplicate with custom designed oligos (Table S3 ...
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bioRxiv - Microbiology 2022Quote: ... expressed using the TNT SP6 High Yield Wheat Germ in vitro transcription/translation system (Promega) in accordance with the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... was generated by in vitro transcription using T7 RNA polymerase (Promega, Charbonnières-les-Bains, France) in presence of Digoxygenin-labeled nucleotides (DIG RNA Labeling Mix ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were produced in vitro via the TNT SP6 Quick Coupled Transcription/Translation System (Promega) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and DNase-I digested before murine leukemia virus (MLV) reverse transcription using random primers (Promega). qPCR was performed using the TaqMan technology (Life Technologies) ...
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bioRxiv - Pathology 2023Quote: ... RNA (1 µg) was reverse transcribed using GoScript Reverse Transcription System (A5001, Promega, Madison, WI). cDNA was measured by quantitative PCR with FastStart Universal SYBR Green Master (Rox ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and reverse transcription was carried out using the Improm-II reverse transcriptase (Promega, Medison, USA) under the conditions described in the kit manual ...
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bioRxiv - Molecular Biology 2023Quote: Proteins for CoIP were produced with the TnT Quick Coupled Transcription/Translation System (Promega, www.promega.com) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... and then reversely transcribed into single-stranded cDNAs by a Reverse-Transcription Kit (Promega, USA). In parallel experiments ...
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bioRxiv - Genomics 2023Quote: Proteins were expressed in rabbit reticulocyte lysate (TNT T7 Quick Coupled Transcription/-Translation System, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... 1 μg of total RNAs was reverse-transcribed using ImProm-IITM Reverse Transcription System (Promega) and genes were analysed with Quantifast SYBR green master mix (Qiagen) ...
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bioRxiv - Cell Biology 2022Quote: ... RNA (1 μg per reaction) was reverse transcribed using the GoScript Reverse Transcription System (Promega). Following reverse transcription ...
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bioRxiv - Cell Biology 2023Quote: ... The circularized RNA was reverse transcribed via the GoScript™ Reverse Transcription System (Promega #A5001). PCR was performed using 2 × Rapid Taq Master Mix (Vazyme ...
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bioRxiv - Genetics 2022Quote: Proteins were produced in vitro via the TNT SP6 Quick Coupled Transcription/Translation System (Promega). If two proteins were to be tested together in a single binding reaction ...
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bioRxiv - Biochemistry 2023Quote: Proteins labeled with [35S]-methionine were produced by coupled transcription and translation reactions (T7, Promega). Translation products were partially purified by ammonium sulfate precipitation [87] ...
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bioRxiv - Plant Biology 2023Quote: ... Reverse transcription was performed using 1μg total RNA and the M-MLV Reverse Transcriptase (Promega). qPCR was performed on a StepOne™ Real-Time PCR System (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA (4 μg) was reverse-transcribed using oligo-dT and the Reverse Transcription System (Promega). cDNA samples were diluted 1:10 and 5 μl of each sample were used for PCR reactions (final volume of 20 μl ...
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bioRxiv - Cell Biology 2023Quote: ... Test proteins were generated in vitro using the TNR quick-coupled transcription-translation kit (Promega). For radiolabeling ...
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bioRxiv - Evolutionary Biology 2024Quote: ... In vitro transcription was performed with T7 RiboMAX Express Large Scale RNA Production System (Promega) according to manufacturer protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... Isolated RNA was then retro-transcribed into cDNA with GoScript™ Reverse Transcription System (Promega) and the expression of the CRISPRi targeted lncRNA genes was assessed through Real-Time PCR with the GoTaq® qPCR Master Mix (Promega) ...
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bioRxiv - Developmental Biology 2024Quote: The cDNA from the isolated RNAs was obtained following GoScript Reverse Transcription System protocol (Promega), using 3 to 4 μg of total RNA for each sample ...
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bioRxiv - Cell Biology 2020Quote: ... total RNA was annealed with 0.1 mg/ml oligo(dT)15 primer (Promega) and cDNA synthesis was performed with M-MLV Reverse Transcriptase (Promega ...
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bioRxiv - Biochemistry 2019Quote: ... activating adaptors were first coupled to 15 µL of Magne HaloTag Beads (Promega) in 2 mL Protein Lo Bind Tubes (Eppendorf ...
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bioRxiv - Biochemistry 2022Quote: ... and then treated for 30 minutes with 15 units of RQ1 DNAse (Promega). The transcribed sgRNAs are subsequently PAGE purified on an 8% denaturing (7 M urea ...
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bioRxiv - Developmental Biology 2020Quote: ... 1μg of RNA was reverse transcribed with oligo(dT)15 Primers (Promega C1101)] using SuperScript III Reverse Transcriptase (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... cells were incubated for 15 min with 500nM Halo TMR ligand (Promega G8251), washed with 2i media three times followed by 30 min incubation in 2i media without the ligand to remove unbound HaloTaq ligands before fluorescence imaging in L-15 medium ...
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bioRxiv - Cell Biology 2021Quote: The medium in 96-well plate was replaced at the indicated time points with 100 μl of PBS supplemented with 0.2 g/l EDTA and 50 μM FITC-VAD-fmk (Promega, USA) or 50 μM 1,1’,3,3,3’,3’-hexamethylindodicarbo-cyanine iodide (DiIC1(5) ...
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bioRxiv - Molecular Biology 2020Quote: ... Two micrograms of total RNA were used for cDNA synthesis with a reverse transcription kit (Promega). Quantitative real-time PCR was performed using the SYBR premix kit (Promega) ...
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bioRxiv - Molecular Biology 2020Quote: ... In vitro translation was performed using a TNT SP6 Quick Coupled Transcription / Translation System (Promega, L2080). 40 μl of rabbit reticulocyte lysates supplemented with methionine were incubated with 1 μg of pKS103-xLIG1 ...
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bioRxiv - Genomics 2019Quote: ... cDNA was synthesized using 200 ng total RNA by the Reverse Transcription kit (Promega, Madison, USA) according to the instruction ...
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bioRxiv - Pathology 2020Quote: ... The extracted RNA (1 μg) was reverse-transcribed using Reverse Transcription System (Promega, Madison, Wisconsin, USA), and cDNAs were amplified using GeneAmp PCR System 9700 (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: Substrate RNAs were synthesized by in vitro transcription using a T7 RNA production system (Promega, P1300) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... and transcribed into complementary DNA (cDNA) with the GoScript Reverse Transcription System (Promega, Madison, WI, USA). cDNA was then amplified with the SYBR Green Master kit (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... [35S]-labeled proteins were synthesized in vitro via a coupled transcription/translation rabbit reticulocyte kit (Promega) incubated at 30°C for 90 min with [35S]Met/Cys metabolic labeling reagent (MP Biomedical ...
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bioRxiv - Microbiology 2022Quote: ... cDNA synthesis was prepared with 1 µg of RNA using an AMV reverse transcription system (Promega) and random primers according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The linearized plasmids were used as templates for in vitro transcription with T3 RNA polymerase (Promega) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... and proteins were expressed with a TNT Quick Coupled Transcription/Translation System (Promega, Madison, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... First-strand cDNA was synthesized from purified RNA with ImProm-II™ Reverse Transcription System (Promega) and diluted 10 times for RT-qPCR to detect related genes with specific primers ...
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bioRxiv - Biochemistry 2021Quote: Mitochondrial 35S-labeled precursor proteins were synthesized using the TNT SP6-coupled transcription/translation kit (Promega) and plasmid-vectors pSP64 containing the genes of interest ...
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bioRxiv - Biochemistry 2021Quote: ... Reverse transcription was performed with 1 unit of Avian Myoblastosis Virus (AMV) reverse transcriptase (Promega®) at 42°C for 2 min ...
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bioRxiv - Developmental Biology 2022Quote: ... first-strand cDNA was synthesized using 1 µg total RNA with MMLV Reverse Transcription Kit (Promega) and poly-T primer ...
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: ... Reverse transcription (RT) was performed with the GoScript™ Reverse Transcriptase kit Random Primers (Promega, A2801) to convert 1 μg of RNA into cDNA ...