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Citations for Promega :
701 - 750 of 1197 citations for Transcription Factor 15 TCF15 Antibody FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... One microgram of total RNA was reverse transcribed using an oligo(dT)15 and the MMLV-RT (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The reaction mixture (15 μl) contained about 7.5 μL of GoTaq® G2 Green Master Mix (Promega, USA), 1.0 μL of primer ...
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bioRxiv - Cancer Biology 2022Quote: Cell viability assays were performed as previously described (15) using CellTiter-Glo® luminescent cell viability assay (Promega). Serial dilutions of commercial drugs or anti-LGR5 ADC were added at the indicated concentrations and allowed to incubate at 37°C for 3 to 4 days ...
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bioRxiv - Microbiology 2020Quote: ... emission 410 nm) was immediately measured every 15 minutes at 37°C using a GloMax plate reader (Promega).
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bioRxiv - Biochemistry 2019Quote: ... Approximately 15 μg of isolated IgG was treated with 0.1 μg of sequencing grade trypsin (Promega, Fitchburg, WI) and incubated overnight at 37 °C ...
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bioRxiv - Genetics 2020Quote: ... PCR was performed in 15 µl reactions using 2x GoTaq DNA Polymerase master mix (Promega Cat. No. M3008) with 5 ng of gDNA and 500 nM of each primer ...
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bioRxiv - Biophysics 2021Quote: ... Human Gβ with a C-terminal 15-amino-acid polypeptide linker followed by a HiBiT (peptide 86, Promega) and human Gγ were cloned into pFastBac vector ...
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bioRxiv - Cell Biology 2021Quote: ... was then used to transcribe 1 µg of total RNA to cDNA using Oligo(dT)15 primers (Promega). KAPA Probe Fast qPCR Master Mix (2X ...
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bioRxiv - Genomics 2021Quote: ... either an oligo(dT)15 primer or a gene-specific primer were used with ImPromII Reverse Transcriptase (Promega). All primers used in RT-qPCR were listed in Supplemental Table 4.
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bioRxiv - Developmental Biology 2021Quote: ... DIG-labeled RNA probes were produced using DIG-UTP by in vitro transcription with T3 RNA polymerase (Promega, Catalog No. P208C) according to the Roche Applied Science’s protocol (Roche Applied Science ...
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bioRxiv - Cell Biology 2020Quote: BBSome subunits and fragments thereof were translated in vitro from pCS2-Myc plasmids using the TNT SP6 Quick Coupled Transcription/Translation system (Promega L2080). 16 µL TNT SP6 Quick Master Mix ...
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bioRxiv - Genetics 2021Quote: ... Approximately 1 μg RNA per sample was converted to cDNA using the ImProm-II Reverse Transcription System kit (Promega, Madison, WI) using the Oligo(dT)15 primer supplied with the kit and with a final concentration of 3 mM MgCl2 according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: hNAA40 and dTIS mutagenized hNAA40 pOTB7 constructs were used as templates for in vitro coupled transcription/translation in a rabbit reticulocyte lysate system according to the manufacturer’s instructions (Promega, Madison, WI) to generate [35S] methionine labeled translation products ...
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bioRxiv - Microbiology 2019Quote: ... Linearized plasmids were used as templates to obtain WT and DI genomic RNAs by in vitro transcription using RiboMAX™ Large Scale RNA Production Systems (Promega). In vitro transcribed RNAs were purified by phenol-chloroform extraction and the quality of purified RNAs was analyzed by electrophoresis on a 1% agarose gel in tris-acetate-EDTA Buffer (TAE).
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bioRxiv - Genetics 2019Quote: For strand-specific RT-PCR of the D and U amplicons cDNA was generated from 150 ng of DNase-treated RNA using the GoScript™ Reverse Transcription System (Promega) with either the Downstream-F or Downstream-R primer ...
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bioRxiv - Systems Biology 2019Quote: Synthesis of proteins was performed separately using the in vitro coupled transcription-translation system based on Insect Cell Extract (ICE) (TnT-T7-ICE, Promega, #L1102). In this ICE coupled transcription-translation system ...
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bioRxiv - Genetics 2019Quote: ... cDNA was synthesised using 1 µg of total RNA in a 20 µL final volume using the GoScript Reverse Transcription System (Promega, USA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... Transcription of tRNApheV and tRNAleuZ was performed with 20 U T7 RNA Polymerase (37 °C for 90 min) using the Riboprobe® in vitro Transcription System (Promega) essentially as described in the instructions manual ...
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bioRxiv - Developmental Biology 2021Quote: ... pCDNA-Cnot3 Δ701-751 and pCDNA-Cnot3Δ651-751 were in vitro transcribed/translated using a TNT® Quick Coupled Transcription/Translation kit (Promega, USA) according to manufacturer’s instructions using 10μCi of 35S-Methionine to radiolabel the proteins ...
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bioRxiv - Cancer Biology 2020Quote: ... 1.0 mcg of linearized purified plasmid were subjected to wheat germ extract in vitro transcription/translation systems employing the non-radioactive Transcend tRNA system according to manufacturer’s instructions (Promega, Madison, WI). 10 of 50uL from the reaction volume was then heat-denatured in the presence of DTT and protein bands were detected by SDS-PAGE gel electrophoresis using a Tris-Glycine 10-20% gel (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were produced using in vitro transcription and translation system using wheat germ extract following manufactures’ instructions (SP6 High Yield Expression System from Promega). Briefly ...
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bioRxiv - Biochemistry 2021Quote: ... import assays of the comparatively higher molecular weight ACE2 and S proteins (50 μL reactions) were both performed using the TNT® Coupled Transcription/ Translation system (Promega) for 90 min at 30°C as described by the manufacturer (∼50 ng/μL cDNA,1 μM Ipom-F or an equivalent volume of DMSO ...
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bioRxiv - Biochemistry 2021Quote: DNA constructs were transcribed and translated in vitro in pairs using equimolar plasmids in 70 μL of TNT® Quick Coupled Transcription/Translation System (Promega). RNaseOUT (0.5 ΜL ...
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bioRxiv - Cell Biology 2021Quote: ... Then a digoxigenin-labeled probe against sox846 was transcribed in vitro by using this linearised plasmid as a template and by following the instructions of a commercial in vitro Transcription System (Promega P1420). Templates for WT α-Tubulin-GFP (Addgene 56450) ...
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bioRxiv - Microbiology 2021Quote: ... and the extracted mRNA was used as a template for reverse transcription using M-MLV reverse transcriptase (Promega, Madison, WI, USA). Virus was quantified using real-time PCR using SYBR Premix Ex Taq™ (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: In vitro translated (IVT) proteins were generated in rabbit reticulocyte lysate using the SP6 TNT Quick-Coupled Transcription/Translation kit (Promega, L2080) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2022Quote: ... Recombinant C-terminally HA tagged Su(H) was produced using in vitro coupled transcription/translation (TNT with rabbit reticulocyte extracts; Promega Inc.) from DNA matrices generated by PCR using Su(H)-HA forward and reverse primers combined with the Su(H ...
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bioRxiv - Evolutionary Biology 2019Quote: ... probe was generated by an in vitro transcription system using primer pairs with T7 and SP6 promoter regions (T7 and SP6 Ribomax™ large scale RNA kit, Promega) (Mlig-pro46 ...
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bioRxiv - Microbiology 2021Quote: ... 5-10 μg DNA templates of T7 promoter-driven BYSMV trailer sequence served as templates for in vitro transcription with the RiboMAXTM Large Scale RNA Production Systems (Promega, P1300) based on the manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2020Quote: ... purified total RNA was reverse transcribed into cDNA by ImProm-II™ Reverse Transcription System (Promega; Madison, WI; Cat. No. A3800). Target gene fragments were first amplified from a mixed cDNA library of planula larva and primary polyps ...
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bioRxiv - Neuroscience 2021Quote: ... reverse transcription of viral RNA followed by quantitative PCR was performed with GoTaq® Probe 1-Step RT-qPCR System (Promega) on a 7500 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2021Quote: Extracted RNA from RSL3-treated cells was converted into cDNA using the Go-Script reverse transcription system (Promega, Madison, Wisconsin, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Linearized plasmids were used as templates to obtain WT and DI genomic RNAs by in vitro transcription using RiboMAX™ Large Scale RNA Production Systems (Promega). In vitro transcribed RNAs were purified by phenol-chloroform extraction and the quality of purified RNAs was analyzed by electrophoresis on a 1% agarose gel in tris-acetate-EDTA Buffer (TAE ...
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bioRxiv - Biochemistry 2022Quote: ... The mRNAs were produced and purified by in vitro transcription using the T7 RiboMAX™ Express Large Scale RNA Production System (Promega) according to the manufacturer’s protocol and subsequent phenol:chloroform extraction and isopropanol precipitation ...
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bioRxiv - Cancer Biology 2022Quote: ... The first strand cDNA was reverse transcribed from the RNA templates using the GoScript Reverse Transcription system kit (Promega, United States) and Oligo(dT)15 primers (Promega ...
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bioRxiv - Microbiology 2023Quote: ... of virus pool stock was used for viral RNA extraction by GeneAid viral RNA extraction kit (GeneAid) and converted to cDNA using ImProm-II™ reverse transcription system (Promega) according to manufacturers’ protocols ...
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bioRxiv - Molecular Biology 2022Quote: ... and 3μg RNA was used to synthesize the first strand cDNA in a 20μl reaction using the Reverse Transcription System kit (Promega, Beijing, China). The GoTaq® qPCR and RT-qPCR Systems kit (Promega ...
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bioRxiv - Microbiology 2023Quote: The effect of pyocin SX2 on protein synthesis was assessed by an in vitro transcription-translation assay (E. coli T7 S30 Extract System for Circular DNA. Promega, Germany). pRL-SV40 vector (Promega ...
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bioRxiv - Plant Biology 2024Quote: Protein expression was carried out using the in vitro transcription and translation system (TnT™ T7 Quick for PCR DNA, Promega) and the EMSA reaction was carried out following manufacturer’s instruction (odyssey) ...
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bioRxiv - Microbiology 2023Quote: ... 1 µg linearized vector was used for in vitro transcription using the T7 RiboMAX™Express Large-Scale RNA Production System (Promega) according to the manual ...
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bioRxiv - Developmental Biology 2023Quote: Pou5f3-3xFLAG 12 and Nanog-HA (kind gift of Nadine Vastenhouw) expression plasmids were in-vitro translated using TnT® Quick Coupled Transcription/Translation Systems (Promega) according to the instructions ...
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bioRxiv - Microbiology 2023Quote: A full-length CVB5 RNA was produced by in vitro transcription of an MluI-linearized plasmid using the T7 RiboMAX™ Express Large Scale RNA Production System (Promega). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... The antiHER2 protein was produced from CD81-antiHER2-tGFP vector (600 ng) using TNT® Quick Coupled Transcription/Translation Systems (Promega), following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription was achieved from 1 𝜇g of total RNA with M-MLV reverse transcriptase (RNase H minus, Point Mutant, Promega, USA) using an anchored oligo(dT)20 primer ...
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bioRxiv - Plant Biology 2023Quote: ... 500 ng-1 µg of the purified template DNA library was used for the in vitro transcription reaction (T7 RiboMAX™ Express Large Scale RNA Production System, Promega). Unincorporated nucleotides were removed using mini Quick Spin RNA Columns (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... the samples were incubated at either 15°C or 37°C for 10 min in Buffer I containing 0.4 U/µL of RNasin (Promega). Following renaturation ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and cDNA synthesis was carried out using an oligo d(T)15 primer and MMLV reverse transcriptase (Promega, USA) enzyme ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA from splicing reactions (∼20 fmol pre-mRNA in 10.5 µL splicing reaction) was analyzed by primer extension (15 µL reaction volume total) using AMV reverse transcriptase (Promega) as previously described (Mayas et al ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were lysed by a 15-min incubation at room temperature with passive lysis buffer (E1941, Promega, Madison, USA). Cell lysates (30 μL ...
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bioRxiv - Developmental Biology 2020Quote: ... with 100 ng of firefly luciferase reporter p12xAT_luc or p12xAT_luc(Mut) (Hojo et al., 2016) 15 ng of the pRL-TK Renilla luciferase control plasmid (Promega) and 100ng (unless otherwise indicated ...