Labshake search
Citations for Promega :
5951 - 6000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... cell lysates were analyzed using the Dual-Luciferase® Reporter Assay System (Promega) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... A GloMAX™ 96 microplate luminometer (Promega) was used to measure the bioluminescence.
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bioRxiv - Genomics 2023Quote: ... Proteins were digested with modified trypsin (sequencing grade, porcine, Promega) in enzyme:protein ratio of 1:50 overnight at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... on day three the media was completely removed and 60 µL lysis buffer together with 60 µL luciferase substrate (Promega) was added per well ...
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bioRxiv - Microbiology 2023Quote: ... chymotrypsin (Mass Spectrometry Grade, Promega) or alpha lytic protease (Sigma Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins captured on the columns were then digested by adding 20 μL of digestion buffer (50 mM TEAB, pH 8.5) containing 1 μg of sequencing grade trypsin (Promega) 1 hour at 47
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bioRxiv - Cancer Biology 2023Quote: ... CellTiter-Glo assay (Promega) was performed ...
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bioRxiv - Cancer Biology 2023Quote: ... Endpoint cell viability was measured using the Cell Titer-Glo luminescent assay kit (Promega) and normalized to day one ...
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bioRxiv - Cancer Biology 2023Quote: ... Membrane-bound antibodies were detected by the enhanced chemiluminescence system (Promega). Primary antibodies were rabbit anti-EGFR (Cell Signaling Technology #4267 ...
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bioRxiv - Microbiology 2023Quote: ... pLenti-LgBiT-T2A-Blast was generated by subcloning the LgBiT coding sequence from the FRB-LgBiT plasmid (Promega N2016) into the lenti-dCas9-VP64-Blast plasmid.
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bioRxiv - Microbiology 2023Quote: ... along with 40 units of Recombinant RNasin (Promega, USA). After confirmation of DNA free preparations using PCR ...
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bioRxiv - Microbiology 2023Quote: ... the RNA was further reverse transcribed to cDNA using MMLV-RT (Promega, USA) and used for downstream mRNA qPCR assays ...
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bioRxiv - Genomics 2023Quote: ... were plated 24-hours before transfection in a 24-well plate with either 100ng WT or MUT pGL2 plasmids (Promega Cat E1631) and 5ng of Renilla luciferase control (Promega Cat E2231) ...
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bioRxiv - Genomics 2023Quote: ... and 5ng of Renilla luciferase control (Promega Cat E2231). Luciferase activity was measured 48-hours post-transfection with the Dual-Luciferase Reporter Assay System (Promega Cat E1910) ...
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bioRxiv - Genomics 2023Quote: ... cells were labeled with HaloTag Oregon Green (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... Individual reactions were combined for each sample and column purified (Promega, A9282). The purified products were then amplified by indexing PCR to add Illumina TruSeq adaptors and sample index sequences with the following primers ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were digested using Trypsin Gold (Promega) in 100 mM HEPES pH 7.6 at 37°C overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... 200 μM dNTPs (Promega, Madison, WI), and forward and reverse oligonucleotides (IDT ...
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bioRxiv - Developmental Biology 2023Quote: ... 200 μM dNTPs (Promega, Madison, WI), and oligonucleotides (IDT ...
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bioRxiv - Developmental Biology 2023Quote: ... 200 μM dNTPs (Promega, Madison, WI), and 3 µM Illumina adaptors ...
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bioRxiv - Developmental Biology 2023Quote: ... 200 μM dNTPs (Promega, Madison, WI), and forward and reverse oligonucleotides (IDT ...
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bioRxiv - Developmental Biology 2023Quote: ... and 0.33µl RNasin® Plus Ribonuclease Inhibitor (40U/ µl) (Promega, Madison, WI). We incubated the solution on ice for 10 minutes ...
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bioRxiv - Cell Biology 2023Quote: Protein-protein interactions between Mkl1/2 and Foxo1/3/4 or glucocorticoid receptors were also measured using NanoBiT PPI Starter Systems (Promega). 293FT cells were seeded in 96-well white wall microplates and co-transfected with the LgBiT and SmBiT plasmids ...
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bioRxiv - Cell Biology 2023Quote: ... and luminescence was measured using the GloMax Discover Microplate Reader (Promega).
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bioRxiv - Cell Biology 2023Quote: ... 1.5 μg of total DNA (1:10 ratio of the Cre plasmid to the fusion protein plasmid) were mixed with 6 μl of Fugene 6 transfection reagent (Promega) in 100 μl of Opti-MEM ...
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bioRxiv - Cell Biology 2023Quote: ... These sgRNA vectors were co-transfected with a dsRED expression vector using ViaFect transfection reagent (Promega #E4981). After 3 days ...
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bioRxiv - Cell Biology 2023Quote: ... FuGene 4K Transfection Reagent (Promega) as well as hemin in a subset of dishes ...
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bioRxiv - Cell Biology 2023Quote: ... Annealed gRNA oligonucleotides were inserted into vector px458 and the gRNA construct was transfected into HeLa cells using ViaFect (Promega) transfection reagent ...
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bioRxiv - Cell Biology 2023Quote: ... the proteins were digested with 1 µg of trypsin/Lys-C mix (Promega) for 16 h at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... and Alkaline Phosphatase-conjugated goat anti-mouse IgG (Promega).
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bioRxiv - Cell Biology 2023Quote: ... It was then resuspended in RNAse (A7973; Promega) 100 µg/mL final concentration in nuclease-free water and incubated for 1 h at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was isolated from the DS-12 isolate using the Wizard gDNA Purification Kit (Promega A1125) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... jejuni NCTC12658 was isolated using Maxwell 16 Tissue DNA extraction kit (Promega) (Illumina sequencing ...
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bioRxiv - Microbiology 2023Quote: ... Chromosomal DNA from the LJ variants was isolated using Maxwell 16 Tissue DNA extraction kit (Promega) according to manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2023Quote: ... We synthesised cDNA with M-MVL reverse transcriptase (Promega) and random hexamer primers (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... A-tailed PCR products were ligated with digested linear pGEM®-T Vector (Promega, Cat. No. A3600), followed by transformation with One Shot™ TOP10 Chemically Competent E ...
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bioRxiv - Developmental Biology 2023Quote: ... After 7 days of culture the MTS cell viability reagent (CellTiter 96® Aqueous One Solution Cell Proliferation Assay, Promega) was added and plates incubated for 4 hours at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Beetle luciferin (Promega) was added to the medium to final concentration of 0.5 mM ...
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bioRxiv - Neuroscience 2023Quote: ... The plasmids were linearized by NotI (Promega) and transcribed to cRNAs using mMESSAGE mMACHINE SP6 transcription kit (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... Chemiluminescence was detected with ECL Western blotting Substrate (Promega, Walldorf, Germany, W1001) using the ChemiDoc MP Imaging System (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... quantifying the ATP production using the CellTiter-Glo Luminescent Cell Viability Assay (Promega) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and 50U/ml of RNasin (Promega, cat# N2611), centrifuged and nuclei were resuspended in 1 ml of washing buffer ...
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bioRxiv - Neuroscience 2023Quote: ... washed with PBS and fluorescence intensity was measured by using the microplate reader GloMax fluorimeter (Promega Corporation, Madison, WI, USA) at the excitation wavelength of 475 nm and emission wavelength 555 nm ...
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bioRxiv - Neuroscience 2023Quote: ... Trypsin (Promega) was added at 1:50 (wt/wt ...
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bioRxiv - Cell Biology 2023Quote: ... Firefly and Renilla luciferase activity was measured in a GloMax Navigator (Promega, #GM2000). Firefly luciferase values were normalized to Renilla luciferase values ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed after exactly 24 hours of stimulation in 1X Passive Lysis Buffer (100 μl per well (Promega, #E1941)) ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA was DNAse treated with RQ1 DNAse (Promega, #M6101). cDNA synthesis was performed using 4000ng RNA using SuperScript IV Reverse Transcriptase (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... HaloTag was labeled with HaloTag-TMR (Promega, G8251) as described in the “Cell culture” section ...
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bioRxiv - Neuroscience 2023Quote: ... or elastase (Promega). Samples were analyzed by nano LC-MS/MS on a Waters M-Class HPLC coupled to a ThermoFisher Orbitrap Fusion Lumos mass spectrometer ...
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bioRxiv - Cell Biology 2023Quote: ... HaloTag was labeled with HaloTag-TMR (Promega, G8251) as described in the “Drug treatments and competition assays” section ...