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Citations for Promega :
5801 - 5850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: FLAG-tagged SPUD in vitro expression was done with Transcend Non-Radioactive Translation Detection System (Promega) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... cells were rinsed and lysed with passive lysis buffer (Promega). Luminescence assay was performed with the Dual-Glo Luciferase Assay System (Promega ...
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bioRxiv - Microbiology 2023Quote: ... Luminescence assay was performed with the Dual-Glo Luciferase Assay System (Promega) following manufacture’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified using PCR cleanup kit (Promega). Sanger sequencing was performed at Psomagen ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted using a DNA Wizard Kit (Promega, UK). Upon extraction ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then lysed and NanoLuc activity measured using the NanoGlo Luciferase Assay System (Promega, Madison, WI, USA). Luciferase activity was normalized to the number of GFP positive cells in each well.
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bioRxiv - Microbiology 2023Quote: ... and a Quantus Fluorometer with Quantifluor ONE dsDNA dye (Promega #E4891).
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bioRxiv - Microbiology 2023Quote: ... CM was then harvested by centrifugation and active caspase-1 measured with the Caspase-Glo 1 Inflammasome Assay (Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... the cleaned PCR product was eluted from the beads in 10 µL of buffer solution containing 10 mM Tris-HCl pH 8.0 with 50 mM NaCl and the DNA concentration was quantified on a Quantus Fluorometer using Quantifluor ONE dsDNA dye (Promega #E4891).
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bioRxiv - Microbiology 2023Quote: ... 3 ml overnight cultures were subjected to DNA extraction using an automated Maxwell® 16 Tissue DNA purification kit and following the manufacturer’s instructions (Promega, UK). For long-read complete genome analysis ...
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bioRxiv - Microbiology 2023Quote: ... The 650 bp positive fragment was purified by Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison, WI, USA).
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bioRxiv - Microbiology 2023Quote: ... The 1800 bp positive amplicons was purified using Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison, WI, USA).
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bioRxiv - Molecular Biology 2023Quote: Ni-NTA beads were firstly digested with 500 ng recombinant Lys-C (Promega) at RT while shaking at 1,400 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... and T7 polymerase (Promega) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... cells were cultured together with the compound for 40 hours before luciferase activity in the infected cells was measured using Nano-Glo Luciferase Assay System (Promega, cat. N1120). Inhibition of SARS-CoV-2 variants RIM-1 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed with clear medium to remove free probe and fluorescence intensity (excitation=485 nm; emission=530 nm) was measured using a microtiter plate reader (Promega, GloMax Microplate reader). In each experimental plate ...
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bioRxiv - Microbiology 2023Quote: ... The amount of dsDNA was quantified using a dsDNA estimation kit with a Fluorometer (Quantiflour, Promega, Madison, USA).
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bioRxiv - Genetics 2023Quote: ... samples were suspended in homogenization buffer (50mM Tris, pH7.5, 100mM KCl, 12mM MgCl2, 1%NP40-substititue, 1mM DTT, 200U/mL Promega RNasin ...
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bioRxiv - Genetics 2023Quote: ... and spiked with 1% unmethylated Lambda DNA (Promega, cat # D1521) to monitor bisulfite conversion efficiency ...
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bioRxiv - Genetics 2023Quote: RNA was isolated from mouse liver using a Maxwell 16 LEV simplyRNA kit (Promega). For the American vs standard diet comparison strand-specific mRNA libraries were generated using the TruSeq Stranded mRNA protocol (Illumina ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and digestion with Trypsin and Lys-C (Trypsin/Lys-C Mix, Mass Spec Grade Promega) for 14 hours at 37° C.
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bioRxiv - Evolutionary Biology 2023Quote: ... 1.00 U of GoTaq DNA polymerase (Promega), 1 ng of template plasmid ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Each 25 μL PCR consisted of 1 x GoTaq Buffer (Promega), 3 μM MgCl2 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Each 50 μL amplification PCR consisted of 1 x GoTaq Buffer (Promega), 3 μM MgCl2 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 1.0 U of GoTaq DNA polymerase (Promega), and 5 ng of each PCR product from the two previous reactions ...
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bioRxiv - Microbiology 2023Quote: ... The samples were then digested with trypsin/LysC (Promega) in the presence of ProteaseMax (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... 400U of RNAsin plus RNase inhibitor (Promega, N261B); 20U of Turbo DNase (Ambion ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were digested by incubation with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) overnight at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... using Random hexamers (Promega, 300453). qPCR reaction was performed in 20ul volume using PowerSYBR Green PCR Master Mix (AppliedBiosystems ...
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bioRxiv - Molecular Biology 2023Quote: The pSV-Rluc renilla luciferase internal standard vector was derived from psiCHECK-2 (Promega, catalog # C8021) by digestion with EagI to remove the firefly luciferase gene ...
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bioRxiv - Microbiology 2023Quote: ... The virus infectivity was determined 48 h later by quantifying the luciferase activity (luciferase assay system, Promega, Madison, WI). Neutralization curves were normalized to virus only control ...
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bioRxiv - Microbiology 2023Quote: ... 100 μL of these purified Se cells or 100 μL of the corresponding Se-Ai cocultures were mixed with 100 μL Nano-Glo Luciferase assay reagent (50:1 mixture of substrate:buffer, Promega N1110) in a 96-well plate ...
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bioRxiv - Microbiology 2023Quote: ... Nl ML1and Ai F0345 was isolated using the Wizard HMW DNA Extraction kit (Promega). Sequencing was performed on Illumina iSeq and MiSeq and Oxford Nanopore (ONT ...
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bioRxiv - Microbiology 2023Quote: ... and 30 µL of BacTiter-Glo reagent (Promega, US) were added to each well ...
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bioRxiv - Microbiology 2023Quote: ... cells were treated with 5 μM Z-VAD-FMK (Promega G7231) or DMSO vehicle control ...
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bioRxiv - Microbiology 2023Quote: ... and 30 µL of BacTiter-Glo reagent (Promega, US) were added to each well ...
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bioRxiv - Molecular Biology 2023Quote: ... The clone was moved into pGL4.10[luc2] (Promega, catalog # E6651) with XhoI and NcoI ...
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bioRxiv - Molecular Biology 2023Quote: ... according to the manufacture’s protocol followed by treatment with RQ1 DNase (Promega) to remove DNA template ...
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bioRxiv - Genomics 2023Quote: ... Amplified cDNA samples were purified with ProNex beads (Promega, WI, USA) using the standard workflow and assuming that most transcripts are ∼2 kb ...
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bioRxiv - Genomics 2023Quote: ... Fluorotect Green BODIPY-FL-labeled charged lysine tRNA (Promega) was added to allow protein visualization ...
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bioRxiv - Genomics 2023Quote: Proteins were expressed in rabbit reticulocyte lysate (TNT T7 Quick Coupled Transcription/-Translation System, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Equal amounts of the lysate were transferred into two Microtiter plates (one for Renilla and the second for Bright Glo (Promega)) ...
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bioRxiv - Molecular Biology 2023Quote: ... and cloned into pGEM-T-Easy (Promega) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... at 42°C for 8 hours in the presence of RNasin Ribonuclease Inhibitor (Promega N2111) and Pyrophosphatase (NEB M0361S) ...
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bioRxiv - Molecular Biology 2023Quote: ... The media mixture was removed from each well and the cells were lysed with a room tempered 1xGlo buffer (Promega) for 5 min at 25°C while shaking at 550 rpm ...
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bioRxiv - Genomics 2023Quote: ... and pGL3-basic (Promega, E1741).
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bioRxiv - Microbiology 2023Quote: The cytotoxic activity on Hela cells of each bacterial strain was analyzed using the CellTiter-Blue® Cell Viability Assay (Promega) following manufacturer instructions ...
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bioRxiv - Genomics 2023Quote: ... Biotinylated RNAs were captured using streptavidin magnetic beads (Promega), washed with salt buffers ...
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bioRxiv - Genomics 2023Quote: ... 0.25 μl of 1% Digitonin in DMSO (Promega (2%), Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... The resultant PCR products were inserted into a pFN21A HaloTag CMV Flexi Vector (Promega) using SgfI/PmeI restriction enzyme recognition sites ...