Labshake search
Citations for Promega :
5451 - 5500 of 8279 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 100 μl of ATP-depleted faecal suspension was mixed with 100 μl BacTiter-Glo™ Microbial Cell Viability Assay (Promega) in a clear ...
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bioRxiv - Genomics 2022Quote: ... Firefly (transfection control) and Renilla (target) luciferase activities were sequentially measured using the Dual-Glo Luciferase Assay System (Promega E2920) and detected on a Spectramax M plate reader (Molecular Devices) ...
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bioRxiv - Immunology 2022Quote: ... and a freeze-thay cycle and Firefly Luciferase activity was assessed using the Firefly Luciferase Assay following the manufacturer’s recommendations (E4550 from Promega, Belgium). Percent infection was calculated relative to infected cells in the absence of test serum ...
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bioRxiv - Immunology 2022Quote: ... cells were treated with experimental media containing metabolites and drugs as described in figure legends for 16 hours and then luciferase signal assessed using the Steady-Glo luciferase assay system (Promega) detected using a luminometer ...
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bioRxiv - Immunology 2022Quote: ... or HT1080/Ace2 cl14 cells (for all variant neutralization assays) for 48 hours after which cells were washed with PBS and lysedwith Luciferase Cell Culture Lysis 5× reagent (Promega). Nanoluc Luciferase activity in lysateswas measured using the Nano-Glo Luciferase Assay System (Promega ...
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bioRxiv - Neuroscience 2022Quote: ... supernatant was collected and protein concentration quantified using the 660 nm Protein Assay Reagent (Pierce) supplemented with 50 mM Ionic Detergent Compatible Reagent (Pierce) in a GloMax Multi Detection Plate Reader (Promega). After boiling in 1X Laemmli Sample Buffer (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... Cell viability was assessed using a Cell Titer 96 AQueous One Solution Cell Proliferation Assay (Promega Corp, Madison, Wisconsin, USA). Absorbance measurements at 490 nm were normalized to the maximum read per cell line ...
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bioRxiv - Immunology 2022Quote: ... Plates were scored for CPE five days post infection using the CellTiter-Glo® Luminescent Cell Viability Assay per the manufacture’s protocol (Promega). TCID50 was calculated using the Reed and Muench method (67).
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bioRxiv - Synthetic Biology 2022Quote: ... Expression of Firefly luciferase (FLuc) and Renilla luciferase (RLuc) were determined with the Dual-Glo® Luciferase Assay System (Promega) following manufacturer’s instruction with some modifications ...
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bioRxiv - Microbiology 2022Quote: ... The cells were incubated for 24 hrs at 37 °C (for HEK-293T) or 39 °C (DF-1) and luciferase activity was measured by using the Dual-Glo luciferase assay system (Promega). The polymerase activity was calculated by normalising firefly luciferase activity relative to the Renilla luciferase activity.
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bioRxiv - Microbiology 2022Quote: Cell viability of different 2D cultures at different time points of infection or exposure to virus proteins or HAE supernatant was determined using the CellTiter 96 AQueous One Solution cell proliferation assay (G3582; Promega), while HTO viability was determined by the CellTiter-Glo 3D cell viability assay (Promega G9681 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Kinase activity of phosphorylated CDK2 with cyclin A and varying amounts of inhibitor (dinaciclib or 5) were measured using the ADP-Glo Kinase Assay (Promega) in a luminescence plate reader (Tecan Infinite M1000 PRO) ...
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bioRxiv - Microbiology 2022Quote: Purified recombinant His6-SBP-tagged HipBLp, HipSLp, and HipTLp (WT, D197Q, D219Q) were tested for ATP hydrolytic activity using the ADP-Glo kinase assay (Promega) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... used as PCR template and directly for luminescence quantification according to the manufacturer’s instructions (20 μL phage plus 20 μL substrate, Nano-Glo® Luciferase Assay System, Promega).
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bioRxiv - Molecular Biology 2023Quote: ... and firefly luciferase (FLuc) activities were measured by using the Nano-Glo Dual Luciferase Assay on a Glomax Multidetection System (Promega). GFP fluorescence was measured using an Infinite M1000 microplate reader (Tecan Group Ltd) ...
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bioRxiv - Plant Biology 2023Quote: ... Luciferase activity was measured combining by combining 20 μl of sample with 50 μl of enzyme substrate (Luciferase 1000 Assay System, #E4550, Promega), vortexing sample briefly and counting photons for 5 s in the luminometer (Luminoskan TL plus ...
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bioRxiv - Microbiology 2022Quote: ... We estimated the number of infectious virus after two days by quantifying the luminescence in each well using Bright Glo Luciferase Assay System (Promega). Differences in relative luciferase units (RLUs ...
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bioRxiv - Molecular Biology 2022Quote: ... FLuc and RLuc activities in lysates were sequentially measured by using Dual-Glo luciferase assay reagents and a GloMax 20/20 luminometer (Promega) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... and dual-luciferase assay performed using homemade reagents (as described in Baker et al., 2014) on the GloMax Discover GM3000 (Promega).
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bioRxiv - Microbiology 2022Quote: ... cells were harvested 24 hours post transfection using 1X passive lysis buffer and the dual luciferase assay was performed according to instructions from manufacturer (Promega). Luciferase signals were read using a BioTek Synergy H1 plate reader (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... Cells were harvested 24 h of post-transfection and RLuc and FLuc activities were measured by Dual Glo luciferase assay (Promega), according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: The proliferation of Ba/F3 expressing WT or mutants TpoR mutants was assessed by Cell-Titer-Glo luminescent cell viability assay (Promega). Prior to the experiment ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were lysed and the activities of both Renilla and Firefly luciferases measured on a plate reader using the Dual Luciferase Assay System (Promega). For each cell line quantifications were done in technical triplicates ...
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bioRxiv - Cancer Biology 2022Quote: ... and luciferase activity was measured after 48 h using the dual-luciferase reporter assay system and GloMax 20/20 (Promega). pRL-null Renilla plasmid was cotransfected at 0.4 pg/μL to control for transfection efficiency ...
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bioRxiv - Cancer Biology 2022Quote: The cell viability was measured by MTS assay according to the manufacturer’s protocol (Cat. No. G-111, Promega, Madison, WI) and as described previously16,26 ...
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bioRxiv - Biochemistry 2022Quote: ... with 10 ng pGL4.32[luc2P/NF-κB-RE/Hygro] (NF-κB response element-dependent firefly luciferase) or pGL4 [luc2P/AP-1-RE/Hygro] and 5 ng pRL-TK using the Dual Luciferase Reporter Assay (Promega). 24 hours post transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... then cell viability was evaluated in culture based on quantification of the ATP present using CellTiter Glo Luminescent cell viability assay (Promega). Raw luminescence was recorded using a CLARIOStar (BMG Labtech ...
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bioRxiv - Cell Biology 2022Quote: Cell viability was determined by the using CellTiter-Glo 2.0 Cell Viability Assay according to the manufacturer’s instructions (Promega, cat # G9241). Unless otherwise indicated ...
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bioRxiv - Biophysics 2024Quote: ... cells were washed once with 0.1 mL phosphate-buffered saline (PBS) and luciferase activity was measured with Dual Luciferase Reporter Assay System (Promega, E1910) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... Four microliters of CFPS sample were added to 30 μL of ONE-Glo Luciferase Assay System (Promega, Madison WI, USA) in a white 96-well plate (Costar 3693 ...
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bioRxiv - Microbiology 2023Quote: ... The viability of Vero cells treated with 5-FU and EIDD-1931 alone were assessed using CellTiter-Glo Viability Assay (Promega).
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bioRxiv - Synthetic Biology 2024Quote: ... per well and then 25 μl PBS and 25 μl of the Nano-Glo® Luciferase Assay System reagent (Promega) were added ...
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bioRxiv - Physiology 2024Quote: ... Proliferation rate of NIH-3T3 fibroblasts was determined 24 h after MNP treatment via MultiTox-Fluor Multiplex Cytotoxicity Assay (Promega). As controls ...
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bioRxiv - Molecular Biology 2024Quote: ... The phase separation assay was performed in the presence or absence of 10% polyethylene glycol 8000 (PEG-8000, Promega, V3011) and the reaction mix loaded in a home-made imaging chamber consisting of a glass slide attached to a cover slip by two parallel strips of double-sided tape ...
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bioRxiv - Immunology 2024Quote: ... Cells were collected 24-hr post transfection and firefly and renilla luciferase were analyzed with the Dual Luciferase Reporter Assay system (Promega). Normalization was performed using Western blot analysis for protein concentration ...
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bioRxiv - Molecular Biology 2024Quote: ... The promoter activity was measured based on the production of luminescence by using the Dual-Luciferase Reporter Assay system (#E1910, Promega) and the Glomax Multi+ Detection System (Promega) ...
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bioRxiv - Molecular Biology 2024Quote: ... at 48-72 hrs post-transfection and lysates were assayed for luciferase activity using the dual-luciferase reporter assay system (Promega) and a luminometer ...
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bioRxiv - Microbiology 2024Quote: ... ATP-depleted uRBCs were then washed three times with PBS and ATP-depletion was evaluated for each dilution using the CellTiter-Glo→ Luminescent Cell Viability assay (Promega) following the instructions provided in the kit (see Extended Data Fig ...
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bioRxiv - Microbiology 2024Quote: ... Lactic acid production was assessed for each representative strain after 24 hours incubation in the same conditions as for the growth assessment using the Lactate-Glo Assay (Promega) following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2024Quote: ... Reactions were incubated for 1.5 hr at 30 °C and luciferase signal was measured using the Dual-Glo Luciferase Assay System (Promega E2920).
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bioRxiv - Microbiology 2023Quote: ... transfected cells were treated with human IFN-γ at (100 U/mL for 24 hr) and firefly luciferase activity was determined using the dual luciferase reporter assay system (Promega) on SpectraMax iD3 (Molecular Devices ...
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bioRxiv - Cancer Biology 2024Quote: ... The activity of caspase 3/7 was measured after 48 h using the Caspase-Glo® 3/7 assay (Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The hydrolase activity of the UDP-substrate was detected as the release of UDP by the UDP-Glo assay (Promega) after 20 min of incubation with UDP-Glo detection reagent ...
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bioRxiv - Cell Biology 2024Quote: ... Reporter activity was analyzed 48 h post-transfection using the Dual luciferase reporter assay system and GloMax 20/20 Luminometer (Promega) instrument ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: β-arrestin2 recruitment to human chemokine receptors in response to 1 µM VUF15485 or 200 nM positive control chemokines was also monitored by NanoLuc complementation assay (NanoBiT, Promega) (Dixon et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was synthesized using Superscript III Reverse Transciptase (#18080044, ThermoFisher Scientific. qPCR assays were performed on the cDNA using Gotaq Green Master Mix (Promega) following the manufacturer’s instruction and read using CFX96 connect (BioRad) ...
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bioRxiv - Genomics 2024Quote: ... The final DNA yield (17.3 µg) was quantified using a Quantus Fluorometer (QuantiFluor ONE dsDNA Dye assay; Promega, Madison, WI). The size distribution of the HMW DNA was estimated using the Femto Pulse system (Agilent ...
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bioRxiv - Immunology 2024Quote: ... cells were lysed in native lysis buffer and luciferase activity was assayed using the Dual-Luciferase Reporter Assay (E1910, Promega) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... The presence of released LDH in the supernatant was assessed using the Cytotox 96 Non-Radioactive Cytotoxicity Assay (Promega, G1780). The extended protocol can be found in the supplementary materials.
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bioRxiv - Bioengineering 2023Quote: ... luminescence was read by removing 150 µL of media and adding 50 µL of substrate from the Nano-Glo Luciferase Assay System (N1110, Promega). The mixture was incubated for 3 minutes in a white-walled plate before analysis with a SpectraMax M3 plate reader (Molecular Devices).