Labshake search
Citations for Promega :
5701 - 5750 of 8279 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... were assayed in white 96 well plates in triplicate by the addition of 50 μl Bright-Glo™ Firefly luciferase assay system (Promega) or freshly prepared 40 ng/ml Coelenterazine in Renilla-Glo buffer (Promega) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cell proliferation and viability were measured at 72 hours post treatment using the CellTiter Blue Viability Assay (Promega, Fitchburg, WI, USA) as described previously (24) ...
-
bioRxiv - Cell Biology 2021Quote: ... bright field images of all plates were acquired with a high-throughput high-content imaging system (Operetta, Perkin-Elmer) and CellTiter-Glo luminescent cell viability assay (Promega, G7570) was performed ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... transduction efficiency was quantified by measuring the activity of firefly luciferase in cell lysates using a luciferase assay system (Promega, USA) and SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices ...
-
bioRxiv - Synthetic Biology 2021Quote: HEK cells were transfected as previously described for the SRE reporter assay with the exception of substituting the SRE reporter with CRE-dependent firefly luciferase 60 (Promega; E8471).
-
bioRxiv - Microbiology 2020Quote: ... or human IFN-β (1000 U/ml) and firefly luciferase activity was determined using the dual luciferase reporter assay system (Promega) on Cytation 3 (BioTek ...
-
bioRxiv - Microbiology 2020Quote: ... pseudovirus titres were measured by luciferase activity in relative light units (RLUs) (Bright-Glo Luciferase Assay System, Promega Biosciences, California, USA). Neutralization assays were performed by adding 100 TCID50 (median tissue culture infectious dose ...
-
bioRxiv - Microbiology 2021Quote: ... The migrated cells in the lower chamber were stained with 5 μl of CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega # G3580) for 2 h at 37° C with 5% CO2 ...
-
bioRxiv - Microbiology 2021Quote: ... At the indicated time points firefly and Renilla luciferase activities were measured using the Dual-Glo® Luciferase assay system (Promega) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... Nanoluciferase activity was measured at 24 or 48 hours post-transfection (hpt) using the Nano-Glo® Luciferase Assay System (#1120, Promega) and a Berthold Centro XS3 luminometer (integration time 1s/well using the MikroWin® software) ...
-
bioRxiv - Developmental Biology 2020Quote: ... Four hours after transfection the tracheal mesenchyme was induced using day5 medium in presence or absence of Wnt agonist (3μM CHIR99021) and cells were cultured for 24 hours and then lysed and assayed using Dual-Luciferase Reporter Assay System (Promega, E1980).
-
bioRxiv - Microbiology 2021Quote: ... Cell viability was measured 48h later (or otherwise indicated) using CellTiter 96® AQueous One Solution Cell Proliferation Assay (G3582, Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2021Quote: ... cells were lysed and activity of firefly and renilla luciferase was measured with a Dual-Luciferase Reporter Assay System (Promega, # E1910).
-
bioRxiv - Microbiology 2021Quote: ... Cells were infected at a MOI of 0.2 as determined by preliminary experiments to generate an ideal dynamic range of the colorimetric CytoTox 96 ® Non-Radioactive Cytotoxicity Assay (Promega). Infections progressed for 72 h at which time supernatant from treatments and controls were processed for LDH release according to the manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2020Quote: ... the culture media were harvested and Nanoluc activity was determined in 10 µl aliquots of culture medium using a Nano-Glo™ Luciferase Assay System (Promega). To normalize the electroporation efficiency ...
-
bioRxiv - Microbiology 2020Quote: ... and Renilla luciferase (Rluc) and firefly luciferase (Fluc) activities were evaluated using the Dual-Luciferase Reporter (DLR™) Assay System (Promega).
-
bioRxiv - Cell Biology 2021Quote: Apoptotic cell death was measured in a 96-well format using the RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay (Promega) per the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... Cell viability was measured with the RealTime-Glo™ MT Cell Viability Assay using the GloMax® 96 Microplate Luminometer (Promega). Luciferase activity was measured with the ONE-Glo™ Luciferase Assay System using the GloMax® 96 Microplate Luminometer according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2021Quote: ... The cell proliferation was assessed 5 days after treatment by measuring viable cells in culture based on quantitation of the ATP present using CellTiter-Glo® Luminescent Cell Viability Assay (Promega) according to the manufacturer’s instructions using a Victor X5 Multi-label plate reader luminescence protocol (Perkin Elmer ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were then cultured overnight before measurement of luciferase and renilla reporter signals using a Dual-Luciferase® Reporter assay system (Promega) and a GloMax® Discover Microplate reader (Promega ...
-
bioRxiv - Physiology 2022Quote: ... the cells were washed with PBS and harvested to determine firefly and Renilla luciferase activities according to the Dual-Luciferase reporter assay system (Promega, USA) on a luminometer (GloMax 20/20 ...
-
bioRxiv - Genomics 2022Quote: ... F-luciferase and R-luciferase expression was assayed in a TECAN Spark plate reader using the Dual-Glo® Luciferase Assay System (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2022Quote: ... Twenty-four hours post treatment the cells were lysed and analyzed for luminescence using the Luciferase Assay system (Promega; Cat #E1500). Normalization among different technical replicates and conditions was carried out by normalizing the relative luminescence units (RLUs ...
-
bioRxiv - Molecular Biology 2022Quote: BD BioCoat Poly-D-Lysine 96-well plates with black sides and clear bottoms were used to run the ApoTox-Glo™ Triplex Assay (Promega). Cells were trypsinized and removed from their culture flasks and diluted to 24,000 cells/ml and 100µl of each sample was added to each well ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were transferred back to the incubator and luciferase was measured at 24 hours post-infection (Bright-Glo™ Luciferase Assay System, Promega). Values were normalized to those derived from wells with pseudovirus but without sera (100% infection) ...
-
bioRxiv - Microbiology 2022Quote: ... Lysates were used to measure the activity of F-Luc and R-Luc via the Dual-Luciferase Reporter Assay System (Promega, E1960) in a GloMax 20/20 luminometer (Promega ...
-
bioRxiv - Molecular Biology 2022Quote: Luciferases were assayed by their activity in oxidizing luciferin with emission of luminescence using the Dual-Luciferase Reporter Assay System (Promega, USA) according to the producer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: ... 48 h later assays were then lysed and read on a FLUOstar Omega plate reader (BMF Labtech) using the Steady-Glo® luciferase assay system (Promega)
-
bioRxiv - Immunology 2022Quote: ... centrifuged at 1,500 × g for 10 min and the viral titers were measured by luciferase activity in relative light units (RLU) (Bright-Glo Luciferase Assay System, Promega, E2620). The supernatants are aliquoted and stored at -80°C until further use ...
-
bioRxiv - Immunology 2022Quote: ... The IC50 values were calculated based on the reduction of 50% relative light units (Bright-Glo Luciferase Assay Vector System, Promega, USA) compared to the virus-only control ...
-
bioRxiv - Microbiology 2021Quote: ... and nanoluciferase activity was measured using Tristar multiwell Luminometer (Berthold Technology) for 2.5 seconds by adding 20 µl of Nano-Glo® substrate in nanoluc assay buffer (Promega Inc). An uninfected monolayer of Vero E6 cells treated identically served as controls for determining background and obtain normalized relative light units ...
-
bioRxiv - Cell Biology 2021Quote: Release of LDH in the cell supernatant was assessed as a marker of cell death in male and female PTECs using the CytoTox96® Cytotoxicity Assay (Promega) following the manufacturer instructions (see Supplemental Methods).
-
bioRxiv - Developmental Biology 2021Quote: ... cells were lysed and analyzed for firefly and Renilla luciferase activities according to the procedure outlined in the Dual-Glo luciferase assay system (Promega #E2920). All luciferase assays were performed in triplicates and repeated at least three times ...
-
bioRxiv - Microbiology 2020Quote: ... SCoV2 infected cells were collected and luciferase activity was then measured using a Nano-Glo HiBiT lytic assay system (Promega, USA), according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... and cells with approximately 70-90% confluency in each plate were transfected with required plasmid(s) for each assay using Fugene 6 transfection reagent (Promega, USA) or 1 mg/ml PEI (polyethyleneimine).
-
bioRxiv - Microbiology 2021Quote: ... were added to 384-well plate wells containing 10 μM of each compound and were incubated with 10 μL of Nano-Glo luciferase assay reagent (Promega Corporation) at 22°C for 10 min and then read using a Perkin-Elmer Enspire plate reader at 460 nm ...
-
bioRxiv - Plant Biology 2021Quote: ... RT-qPCR assays were carried out in triplicate using SYBR® Green chemistry with GoTaq® qPCR Master Mix (Promega, UK) on a StepOne™ Real-Time PCR Machine (Applied Biosystems ...
-
bioRxiv - Neuroscience 2021Quote: The Pgp-Glo™ assay was performed by following our previously described method22 and using the manufacturer’s instructions (Promega, Co. USA). Briefly ...
-
bioRxiv - Microbiology 2021Quote: ... Cell viability of primary human nasal epithelial cells was analyzed during inhibitor only treatment at highest concentrations (50 uM, 60 uM) using the CellTiter-Glo® 2.0 Cell Viability Assay (Promega, G9241) and related to DMSO treated cells.
-
bioRxiv - Neuroscience 2022Quote: ... astrocytes were collected and processed after a 2-hour incubation period to determine intracellular glutamate levels using the Glutamate-Glo™ Assay (Promega) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... Cell viability was measured 72 hours post-infection by adding 100μl of CellTiter-Glo reagent to each well as described in the manufacturers protocol (CellTiter-Glo Luminescent Cell Viability Assay, Promega # G7571). Luminescence was quantified using the Infinite M200Pro TECAN apparatus.
-
bioRxiv - Cell Biology 2022Quote: The number of viable cells inside HTM hydrogels subjected to the different treatments for 10 d was measured with the CellTiter 96® Aqueous Non-Radioactive Cell Proliferation Assay (MTS; Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... Pseudoviruses stock were titrated by infecting 293T-ACE2 cells and luciferase activity was determined following a 44-48 h incubation period at 37°C and 5% CO2 by addition Bright-Glo Luciferase Assay System (Promega, E2650) using a microplate reader (TECAN ...
-
bioRxiv - Cancer Biology 2022Quote: ... treated as indicated for 72 h and assessed for cell viability using the Cell Titer-Glo® Luminescent Cell Viability Assay (Promega) or Cell Counting Kit-8 (CCK-8 ...
-
bioRxiv - Pathology 2020Quote: Cell viability was assessed using the CellTiter-Glo® Luminescent Cell Viability Assay following the manufacturer’s protocol (Promega, Madison, WI, USA). The luminescence was recorded using an Infinite F200 Pro plate reader (Tecan ...
-
bioRxiv - Microbiology 2021Quote: ... cell viability was determined by measuring LDH release using the CellTiter 96® Aqueous Non-Radioactive Cell Proliferation Assay (Promega, MI) as per manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: Stimulation of P-gP ATPase activity was utilized to indicate the interaction between peptides and P-gP using the P-gP-Glo Assay System (Promega, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cells were incubated for 4 days and cell viabilty was measured using CellTiter-Glo® Luminescent Cell Viability Assay (Promega).
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... cellular lysates were assayed for luciferase and Renilla activities using the Dual-Luciferase Reporter assay system (E1980, Promega, Madison, WI, USA). Luminescence was measured using Glomax 20/20 luminometer (Promega ...
-
bioRxiv - Cancer Biology 2020Quote: ... human recombinant NRG-1β (4711, BioCat) or the different CM on cell viability was evaluated using CellTiter-Glo® luminescent assay (G7570, Promega). Prior to addition of CM or NRG-1β (50ng/ml) ...