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Citations for Promega :
5301 - 5350 of 8279 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The cell lysates were transferred to a 96-well black plate and then an equal volume of Luciferase assay reagent (Promega) was added ...
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bioRxiv - Cancer Biology 2020Quote: ... 72 and 96 hours with a Tecan Infinite M200PRO reader using the CellTiter-Glo® Luminescent Cell Viability Assay according to manufacturer’s directions (Promega). For WCM154 cells ...
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bioRxiv - Cancer Biology 2020Quote: ... and measured for reporter activity by mixing 50 μl of ONE-Glo EX Luciferase Reagent and NanoDLR Stop & Glo Reagent sequentially as described in the Nano-Glo Dual Luciferase Assay System manual (Promega).
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bioRxiv - Cell Biology 2021Quote: ... Firefly and renilla luciferase activities were measured using an Optocomp 1 luminometer and a Dual Luciferase Reporter Assay System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... R-Luc and F-Luc activities were measured 48 h after transfection using the Dual-Luciferase Reporter Assay System (Promega). Renilla luciferase activity was normalized to firefly luciferase.
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bioRxiv - Cell Biology 2021Quote: ... the luciferase activities were measured in 20 μl of cell-lysate using the dual-luciferase assay system (Promega, Madison, WI) with a luminometer (LumiCount ...
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bioRxiv - Cell Biology 2021Quote: ... After 24 hours incubation with Wnt3A CM the cells were harvested in passive lysis buffer and both Firefly and Renilla luciferase activity was measured in the same sample using the Dual-Luciferase Reporter Assay System (Promega). The luminescence was quantified on a GloMax-Multi microplate reader (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... Cell death was measured 24 hours post seeding via luminescence using the Caspase Glo assay (Promega, Madison, WI, USA: #G8090). Annexin-V /7AAD staining (Biolegend ...
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bioRxiv - Microbiology 2020Quote: Apoptosis induction by T3DC and inhibition by Z-VAD-FMK was determined using the Caspase-Glo 3/7 Assay System (Promega). PC3 cells were plated at 1 × 104 cells per 96 well plate and 24 h later were either treated with docetaxel ...
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bioRxiv - Microbiology 2020Quote: ... The infected cells were harvested at 8hpi and the viral NLuc activity was measured by using Nano-Glo® Luciferase Assay System according to the manufacturer’s instructions (Promega) and the luminescence was detected by using a Luminometer (Glomax 20/20 ...
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bioRxiv - Plant Biology 2021Quote: ... The GST-PIF3 and GST-PIF3mTAD constructs for GST pulldown assays were generated by subcloning PIF3 or PIF3mTAD into the EcoRI/XhoI sites of the pET42b vector (Promega). The PIF3p::HA-YFP-PIF3 and PIF3p::HA-YFP-PIF3mTAD constructs were generated by subcloning 2-kb PIF3 promoter ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were harvested 24 hours post-transfection and RLuc and FLuc activities were measured by Dual Glo luciferase assay (Promega), according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: ... The samples were sonicated for 30 seconds before centrifugation and 5 μl of the supernatants were collected for luciferase expression reading by the dual-luciferase reporter assay system (Promega) using a GLOMAX 20/20 luminometer (Promega) ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were treated in triplicates with increasing doses of in-house small molecule inhibitor drugs and a DMSO vehicle control for 3 days and subsequently assessed for cell viability by measurement of ATP with CellTiter-Glo Luminescent Cell Viability Assay (Promega). Signal intensity was read on a Glomax™ 96 Microplate Luminometer (Promega ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... at room temperature, with 40 μL assay buffer (CO2-independent medium, 10% FBS) supplemented with 2% (v/v) GlosensorTM reagent (Promega). To stimulate endogenous cAMP via β adrenergic-Gs activation ...
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bioRxiv - Plant Biology 2021Quote: ... the activities of the Firefly and NanoLuc luciferases were measured in a TriStar2 LB942 luminometer (Berthold) using the ONE-Glo Luciferase Assay System (Promega) and the Nano-Glo Live Cell Assay System (Promega).
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bioRxiv - Microbiology 2021Quote: ... The cells were transferred to white- walled 96-well plates and processed using the Bright-Glo Luciferase Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: The effects of inhibitors as single agent on viability of PBMCs was measured using CellTiter-Glo assay (Promega, WI, USA) as described previously3 ...
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bioRxiv - Molecular Biology 2021Quote: ... lysates were transferred into white 96-well plates and FLuc activity was measured using a commercial substrate (Beetle-Juice, PJK; Luciferase Assay System, Promega) and a plate luminometer (Hidex Sense Plate Reader ...
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bioRxiv - Microbiology 2020Quote: ... MTS assays were performed to measure the cell viability using CellTiter 96 AQueous One reagent (G3580, Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... cytotoxicity of Bordetella infection was measured as lactate dehydrogenase (LDH) release into cell culture media using CytoTox 96 assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then left at 32°C or moved to 37°C for 9 hours before read on a luminometer using the Dual-Luciferase Reporter Assay Step (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the transduction efficiency was quantified by measuring the firefly luciferase activity in cell lysates using the firefly luciferase substrate of the dual-luciferase reporter assay system (Promega) and a Glowmax plate luminometer (Promega).
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bioRxiv - Microbiology 2020Quote: ... and luciferase activity was measured in 3 µl aliquots of the cell lysates using a Dual-Luciferase Reporter Assay System (Promega). Firefly luciferase activity was normalized with that of Renilla luciferase.
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bioRxiv - Microbiology 2020Quote: ... at 9 h post-electroporation and luciferase activity was measured in 10 µl aliquots of the cell lysates using a Luciferase Reporter Assay System (Promega).
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bioRxiv - Cancer Biology 2020Quote: HF3016 cells were plated in individual wells of a 96-well plate and viable cells were quantified using CellTiter-Glo 3D Cell Viability Assay (Promega) in triplicate wells as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Short term cell viability was assessed using the Cell Titer 96 AQeous One Solution MTS cell proliferation assay (Promega, UK), as previously described {Beeby ...
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bioRxiv - Cancer Biology 2020Quote: ... and extracts were assayed in technical triplicate for firefly and renilla luciferase activity using the Dual Luciferase Reporter Assay (Promega) and a Synergy H1 Hybrid Multi-Mode Microplate Reader (Biotek ...
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bioRxiv - Cell Biology 2020Quote: ... Luciferase activity was measured 60 h post-transfection using the Dual-Luciferase® Reporter Assay System (Promega GmbH, Mannheim, Germany) on an Infinite® M200 Pro (TECAN ...
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bioRxiv - Cell Biology 2020Quote: ... the release of LDH was monitored from an aliquot of 250 μL supernatant using the LDH-Glo cytotoxicity assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... The quenched reactions were then transferred to a 384-well white round bottom microplate to profile for methyltransferase activity (cumulative mono-, di- and tri-methylation) using the MTase-Glo assay (Promega), which detects the production of S-adenosyl homocysteine (SAH ...
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bioRxiv - Cancer Biology 2021Quote: ... T cells was detected by co-culturing with MDA-MB-231 GFP-Luc and Hep3B GFP-Luc at various E/T ratios for 24 hours or 96 hours followed by measurement of the luciferase activity using the luciferase assay system (Promega) on Victor (PerkinElmer) ...
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bioRxiv - Cell Biology 2020Quote: Identification of the β5-integrin carboxyl domain as Src substrate was determined using the ADP-Glo Kinase Assay (Promega, V6930) following protocols recommended by the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were cultured for 48 h and the luciferase activity was measured using the Dual-Luciferase Reporter Assay System (Promega). The final firefly luciferase activity was normalized to the co-expressed renilla luciferase activity.
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bioRxiv - Cell Biology 2021Quote: ... luciferase activity was measured at luminometer Glomax 20/20 using Dual-Luciferase Reporter Assay System (cat. #E1910-Promega, Fitchburg, USA) following manufacturer's instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were lysed in passive lysis buffer and Renilla and firefly luciferase activity was measured in duplicates using the Dual-Glo Luciferase Assay System (Promega) according to the manufacturer’s instructions in a GloMax 96 Microplate Luminometer (Promega) ...
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bioRxiv - Cancer Biology 2022Quote: ... 25,000 leukemia cells in 250µL of drug-containing medium were seeded in a 48-well plate and viability was assessed every 4 days by using the CellTiter-Glo Luminescent Cell Viability assay (Promega). For RNA-Seq and ChIP-Seq experiments ...
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bioRxiv - Microbiology 2022Quote: ... was used to measure the luciferase activity of each well after the addition of 50-100 µl of reconstituted luciferase assay buffer (Promega) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Caspase 3/7 activity was assayed according to the manufacturer’s protocol for ApoOne® Homogenous Caspase 3/7 Assay (Promega). Fluorescence was measured with Glomax Multi plate reader (Promega).
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bioRxiv - Cancer Biology 2022Quote: Half-maximal inhibitory concentrations (IC50) of binimetinib (Selleckchem, MEK162) were determined for each cell line using CellTiter-Glo (CTG) luminescent cell viability assay (Promega). Cells were seeded at density of 10,000 (SKMEL2 ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μl of the lysate was used to measure luciferase activity by sequential mixing with 10 μl of each substrate from the Dual Luciferase Reporter Assay System (Promega). Luminiscence was measured on a Mithras LB 940 microplate reader (Berthold Technologies) ...
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bioRxiv - Immunology 2022Quote: Lytic cell death was determined by measuring LDH release from cell-free supernatants using a colorimetric assay (Promega or Takara) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... reduction in cell viability was determined as reduction of cellular ATP levels by using the CellTiter-Glo Luminescent Cell Viability Assay (Promega). Cells were treated with 5 µM nigericin as a positive control for inflammasome activation or 0.5% DMSO as a negative control ...
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bioRxiv - Microbiology 2022Quote: ... the luminescence signals of biosensors and renilla luciferase (an internal control reporter) from pGS vectors were measured by Dual-Glo Luciferase Assay System (Promega). The ratio (firefly luciferase/renilla luciferase ...
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bioRxiv - Plant Biology 2022Quote: ... Firefly luciferase and Renilla luciferase activities were assayed in extracts from leaf disc (1cm in diameter) using Dual-Luciferase Assay System E1910 (Promega) according to manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Media from the basal layer of the tissue were collected at the same time points for LDH cytotoxicity measurements (LDH-Glo Cytotoxicity Assay, Promega). TCID50 assay was performed as previously described [19] ...
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bioRxiv - Microbiology 2022Quote: Cellular surface levels of PS were assessed using Promega’s RealTime-Glo Annexin V Apoptosis and Necrosis Assay (Promega, cat. JA1012) according to manufacturer specifications ...
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bioRxiv - Microbiology 2022Quote: ... was used to measure the luciferase activity of each well after the addition of 50-100 µl of reconstituted luciferase assay buffer (Promega). Inhibitors were normalized to vehicle control.
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bioRxiv - Genomics 2022Quote: ... Cells were harvested 72 hours post-transfection using the Passive lysis buffer (provided with the Dual Luciferase Reporter Assay system (Promega)) ...
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bioRxiv - Biochemistry 2022Quote: ... Pre-B Cell viability was assessed using trypan blue dye exclusion test54 and the CellTiter-Glo® assay (Promega, G7570). For the CellTiter-Glo assay ...