Labshake search
Citations for Promega :
5351 - 5400 of 8279 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2022Quote: ... Lysates were measured for luciferase activity and RLuc using the Dual Glo luciferase assay system on a Glomax reader (both Promega). To assess BMP sensitivity ...
-
Combining LSD1 and JAK-STAT inhibition targets Down syndrome-associated myeloid leukemia at its corebioRxiv - Cancer Biology 2022Quote: ... Cell viability was determined on day 6 using the CellTiter-Glo® 2.0 Cell Viability Assay (Promega, Madison, WI, USA) according to the manufacturer’s recommendations ...
-
bioRxiv - Cancer Biology 2022Quote: ... Treatments were removed 48 or 72 h later and enzymatic activities of caspase-3 and -7 were measured using Caspase-Glo 3/7 Assay (Promega) and read by a microplate reader (SpectraMax M5).
-
bioRxiv - Microbiology 2022Quote: ... Replicon activity was calculated as the ratio of Renilla (subgenomic reporter) to Firefly (co-transfected loading control RNA, cap-dependent translation) using Dual-Luciferase Stop & Glo Reporter Assay System (Promega) and normalized by the same ratio for the control WT replicon ...
-
bioRxiv - Biochemistry 2022Quote: ... cells were lysed and assayed with firefly luciferase and renilla luciferase substrates in the Dual-Luciferase Reporter Assay System (E1980, Promega). Firefly luciferase activity (RLU1 ...
-
bioRxiv - Biochemistry 2022Quote: ... Reactions were diluted if needed to bring the AMP concentration within the linear window of the AMP Glo assay (Promega). The AMP Glo assay was then performed according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2022Quote: LDH and cardiac troponin T release was quantified per manufacturer instructions using Promega LDH-Glo™ Cytotoxicity Assay (Promega J2380) and human cardiac troponin T ELISA kit (Abcam ...
-
bioRxiv - Developmental Biology 2022Quote: ... The activity of the Firefly luciferase was measured and normalized to Renilla luciferase activity using the Dual-Luciferase Reporter Assay System (Promega).
-
bioRxiv - Plant Biology 2021Quote: ... a 10 mm × 10 mm piece of tissue surrounding the inoculation point was applied to the Dual-Luciferase Reporter Assay System (Promega), to detect the firefly luciferase activity (or activation of the ACS2 and ACS2mut promoters) ...
-
bioRxiv - Microbiology 2020Quote: ... Media was changed 24 hours post infection and luciferase levels were measured 48 hours post infection using Steady-Glo Luciferase Assay system (Promega) per the manufacturer’s recommendation and an automated plate reader ...
-
bioRxiv - Microbiology 2021Quote: The hydrolytic activity of immunoprecipitated PDEs bound to anti-HA magnetic beads was measured using the PDE-Glo assay (Promega), a bioluminsescence-based assay which quantifies the amount of cAMP or cGMP hydrolysed by a given PDE ...
-
bioRxiv - Microbiology 2020Quote: ... 20μl of each sample was read on Fluo Star Optima plate reader with injection of 50 μl of Luciferase Assay Reagent (Promega).
-
bioRxiv - Microbiology 2020Quote: ... The 50% tissue culture infectious dose (TCID50) of SARS-CoV-2 pseudovirus was determined using the Steady-Glo luciferase assay system (Promega).
-
bioRxiv - Microbiology 2020Quote: ... The plates were further incubated for 48 hours at 37°C before readout of luciferase reporter gene expression on a Perkin Elmer EnVision Multilabel Reader using the Bright-Glo Luciferase Assay System (Promega).
-
bioRxiv - Microbiology 2020Quote: ... the cells were lysed with lysis buffer and luciferase activity was immediately measured by dual-glo luciferase assay system (Promega). The inhibition rate was calculated as (sera signals – blank control signals)/ (virus signals – blank control signals ...
-
bioRxiv - Microbiology 2020Quote: ... the cells were lysed in passive lysis buffer and luciferase activities of the firefly luciferase and renilla luciferase were determined using the dual-glo luciferase assay system (Promega) and an Orion II microplate Luminometer (Berthold) ...
-
bioRxiv - Plant Biology 2021Quote: ... 20 μl of the supernatant were used to measure the LUC and RLUC activity using a Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions in a Tecan M-100PRO plate reader ...
-
bioRxiv - Microbiology 2021Quote: ... used for FISH-Flow analysis or lysed in passive lysis buffer to measure luciferase activity using Luciferase Assay System (Promega). Relative light units were normalized to protein content determined by Bradford assay (Bio Rad).
-
bioRxiv - Molecular Biology 2020Quote: ... cells were rinsed with ice-cold PBS and Renilla and Firefly luciferase activities were assessed using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... 50 or 100 μl of supernatants was transferred to 96-well plates and ATP was measured using the CellTiter-Glo Luminescent Cell Viability Assay (Promega) according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2020Quote: ... A reporter vector carrying the WT or MUT sequences of IκBα-UTR was assayed for luciferase expression using the Dual-Luciferase® Reporter Assay System (Promega) following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2020Quote: ... 20 μl of cell lysate or medium were subjected to NanoLuc luciferase assay using Nano-Glo Live Cell Reagent containing 1/50 diluted furimazine substrate (Promega). Relative Luminescence Unit (RLU ...
-
bioRxiv - Biochemistry 2020Quote: ... cells were lysed in 60 µl of 1x passive lysis buffer of the Dual-Luciferase Reporter Assay System (Promega, E1980) and directly assayed or frozen at −20°C ...
-
bioRxiv - Microbiology 2021Quote: ... Samples from three technical replicate transfections were measured in duplicate using a Zylux FB12 luminometer and the Dual-Luciferase Reporter Assay System (Promega). Luciferase activity measurements were normalized to total protein concentrations detected in cell lysates ...
-
bioRxiv - Immunology 2022Quote: ... Cells were transferred to a white-walled 96-well assay plate and mixed with an equal volume of Cell Titer Glo reagent (Promega). Measurements for fluorescence at 544-15 nm (excitation) ...
-
bioRxiv - Microbiology 2022Quote: ... Twenty-five microliters of the substrate diluted at 1/20 in buffer (NanoGlo live assay, Promega, see the manufacturer’s instructions) was added extemporaneously ...
-
bioRxiv - Microbiology 2022Quote: ... then cells were harvested and cell lysates were used to determine luciferase using a Dual-Luciferase Reporter Assay System (Promega). The firefly luciferase activities were normalized to Renilla luciferase activities.
-
bioRxiv - Neuroscience 2022Quote: ... the plasmid gene expression under the regulation of both Nogo-A 3’UTRs was evaluated by measuring firefly and Renilla luciferase activities using the Dual-GLO luciferase assay system (Promega) in an Infinite M200 plate reader (Tecan ...
-
bioRxiv - Cancer Biology 2022Quote: HEK293 cells stably expressing pFN31K-Nluc-PAK1 were assayed for luciferase activity according to the manufacturer’s Nano-Glo® Live Cell Assay System protocol (Promega). In brief ...
-
bioRxiv - Microbiology 2022Quote: ... Nluc activity in the cell culture supernatants at the same times post-infection was quantified using a microplate reader and a Nano-Glo Luciferase Assay system (Promega) following the manufacturers’ recommendations ...
-
bioRxiv - Immunology 2022Quote: ... followed by a PBS wash and lysis buffer to measure the luciferase activity in cell lysates using the Nano-Glo Luciferase Assay System (Promega) and GloMax system (Turner BioSystems) ...
-
bioRxiv - Microbiology 2020Quote: ... cell supernatants were used to monitor GLuc levels in Nunc 96-well optical-bottom plates (ThermoScientific) using Dual-luciferase reporter assay system (Promega) as per manufacturer’s protocol while the harvested cell lysates were assayed for GFP levels in Varioskan microplate reader (Thermo Fisher Scientific).
-
bioRxiv - Genetics 2020Quote: ... Cells were lysed 48 hours after transfection and luciferase/Renilla luciferase activity was measured using the Dual-Luciferase Reporter Assay System (Promega). To measure the transcriptional activity of the MMETN element upstream of the Cd59a gene ...
-
bioRxiv - Molecular Biology 2020Quote: ... and activity of both luciferases was measured in 10 μL of the sample with the Dual Luciferase Reporter Assay system (Promega) on a Modulus Single Tube Reader (Turner Biosystems) ...
-
bioRxiv - Cell Biology 2020Quote: Measurements of caspase activities in cells were performed using the commercially available Caspase-Glo 3/7 Assay (Promega, Madison, WI) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were lysed 48 hours after transfection and assayed for luciferase activity using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions using a Flexstation 3 Multi-mode Microplate Reader (Molecular Devices) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Transfected cells were collected in PBS and luciferase activity was assessed using the Nano-Glo Dual Luciferase reporter assay system (Promega). Dual luciferase signal was quantified using a VICTOR-3 plate reader (Perkin Elmer) ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Luciferase activity was measured using a Berthold Centro XS3 LB 960 microplate luminometer with automatic injection of Luciferase Assay Reagent (Promega). Luminescence measured in DMSO only wells was averaged and subtracted from all values on the plate ...
-
bioRxiv - Genomics 2020Quote: ... firefly and Renilla luciferase luminescence was measured using the Dual-Glo® Luciferase Assay System (Promega Corporation, Madison, WI, USA). Firefly luminescence values were normalized to Renilla luminescence and compared to the empty pGL3::PRO vector.
-
bioRxiv - Immunology 2020Quote: ... The activity of Renilla luciferase in total cell lysates was measured with the use of a Dual-Luciferase Reporter Assay System (Promega) and was normalized by that of firefly luciferase derived from 80 ng of a control plasmid.
-
bioRxiv - Microbiology 2020Quote: ... towards HeLa cells was determined as a release of the intracellular enzyme lactate dehydrogenase (LDH) into the cell culture media using CytoTox 96 assay (cat.no. G1780, Promega, USA) or as changes in cell membrane integrity using CellTox Green Cytotoxicity Assay (cat.no ...
-
bioRxiv - Cell Biology 2020Quote: IC50 was determined on days 3 and 4 after vemurafenib treatment using Real-time-Glo MT Cell Viability Assay according to the manufacturer’s protocol (Promega, #G9711). Using a 96-well white plate ...
-
bioRxiv - Molecular Biology 2020Quote: ... Apoptosis was assessed by incubating the cells with 200nM Staurosporin or medium for 4 h and the caspase 3/7 activity was assayed using the ApoOne Caspase 3/7 Assay (Promega). Senescence associated β-Galactosidase activity was analyzed with the Senescence Associated β-Galactosidase Staining Kit (Cell Signalling Technologies) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Luciferase activity present in 10 µl of lysate clarified by centrifugation was then quantitated in white 96-well luminometer plate (Microlumat Plus 1b 96V instrument, Berthold Technologies) using 50 µl of luciferase assay reagent (Promega).
-
bioRxiv - Systems Biology 2020Quote: ... cells were lysed and cellular luciferase activity was measured to evaluate the HCV replication activity with a Luciferase Assay System (Promega) per manufacturer’s protocol (18).
-
bioRxiv - Cancer Biology 2021Quote: ... Cell proliferation was measured every day for up to 72 hours with the CellTiter-Blue assay (#G8080, Promega, Madison, WI) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... The numbers of cells that migrated into the lower chamber as well as those detached by Trypsin-EDTA were quantified by CellTiter-Glo (CTG) assay (Promega). % Migration = the number of cells that migrated in response to chemoattractant / the total number of cells seeded on the chamber.
-
bioRxiv - Developmental Biology 2020Quote: ... the cells for the luciferase assay were exposed to 100 μL of a 1:1 dilution of DMEM and luciferase substrate (ONE-Glo Luciferase Assay System, Promega), and luminescence from each well measured in a GloMax-96 Microplate Luminometer (Promega) ...
-
bioRxiv - Microbiology 2020Quote: ... All drugs were dissolved in dimethyl sulfoxide (DMSO) and their cytotoxicity determined using a lactate dehydrogenase (LDH) assay (Promega, UK).
-
bioRxiv - Microbiology 2020Quote: ... by counting the number of green cells in each well using an Acumen eX3 scanner or by measuring luciferase activity with Steady-Glo Luciferase assay system (Promega) using EnVison (Perkin Elmer).