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Citations for Promega :
5651 - 5700 of 8279 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µL of an aliquot of the reaction mixture was added to 30 µL of the Luciferase Assay Reagent (Promega), and luminescence was measured as translational activity using a luminometer (GloMax ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were treated with ferroptosis inducers and inhibitors for 24 hours and cytotoxicity was measured using Cell Titer Glo2 Assay (Promega) based on the manufacterer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... cells were incubated for 48 h before performing a luciferase assay using Bright-Glo™ Luciferase reagent (Promega, Madison, WI). A BioTek Synergy HTX multimode luminometer (BioTex ...
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bioRxiv - Cancer Biology 2024Quote: ... 95% humidity for 3 days (72 hours. Cell viability was assessed by the CellTiter-Glo luminescent cell viability assay (Promega). SynergyFinder (https://synergyfinder.fimm.fi ...
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bioRxiv - Bioengineering 2024Quote: ... cell survival in each well was quantified on a Tecan Spark plate reader using CellTiter-Glo 2.0 Cell Viability Assay (Promega, G9242). Percent survival was calculated as the ratio of luminescence in the presence versus absence of puromycin for each lentiviral dilution ...
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bioRxiv - Cell Biology 2024Quote: ... After 25 days the total number of colonies was counted in a blinded manner and later also quantified based on an ATP-sensitive luminescent assay (CellTiterGLO, Promega).
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bioRxiv - Bioengineering 2024Quote: ... IC50 concentrations of these drugs were determined by studying their cytotoxic properties using CellTiter-Glo® 2.0 Luminescent Cell Viability Assay (Promega). MDA-MB-231 cells expressing HMGB1-GFP and CRT-RFP (stained MDA cells ...
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bioRxiv - Cancer Biology 2024Quote: ... RLI from transduced cells was added to cytokine-starved CTLL-2 cells with proliferation of these cells subsequently measured via Colorimetric MTS Assay (Promega). Co-culture assays were conducted by incubating SB28 tumor cells with freshly isolated NK cells (BioLegend MojoSort™ NK Cell Isolation Kit ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was assessed after 72 h of treatment by measuring ATP levels using a luminescence-based assay (CellTiter-Glo, Promega) on a Perkin Elmer Envision Multilabel Plate Reader ...
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bioRxiv - Cancer Biology 2024Quote: ... Cellular proliferation was also measured at Day 0 and Day 2 using RealTime-Glo™ MT Cell Viability Assay (Promega).
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bioRxiv - Cancer Biology 2024Quote: Cell viability by loss in ATP content was measured using the CellTiter-Glo Luminescent and CellTiter-Glo 3D Luminescent Cell Viability Assays (Promega). Cells were seeded in duplicates in 100 μL media on a Nunc 96-Well White Microplate (Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... 150μL of media was carefully removed from each well and 50μL of the CellTiter-Glo 3D cell viability assay (Promega, G9682) reagent was added ...
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bioRxiv - Cancer Biology 2024Quote: ... and NIC-PS on the growth of HepG2 and Huh7 cells were assessed using the CellTiter 96 Aqueous One Solultion Cell Proliferation assay (cat# G4100, Promega). Cells were seeded at 3×103 cells per well in 96-well plates and allowed to adhere overnight ...
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bioRxiv - Cancer Biology 2024Quote: Intracellular levels of glutathione (GSH and GSSG) in the cells were determined using the GSH/GSSG-GloTM Assay (Promega, V6611). Cells (HaCaT and Beas-2B ...
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bioRxiv - Genetics 2024Quote: ... as established by assaying total ATP levels (indirect readout of cell grown) during a 6-day treatment using the Cell Titer Glo assay (Promega), as depicted in Fig ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The Renilla luciferase activity associated with WT or uORF-mutated 5’UTRs was measured according to the manual of the Dual-Luciferase Reporter Assay System (Promega) 32 h after transfection and was normalized to the activity of firefly luciferase.
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bioRxiv - Genetics 2022Quote: Genomic DNA was isolated from blood samples using commercial kit (Wizard® Genomic DNA Purification Kit; Promega), following the fabricant protocol ...
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bioRxiv - Plant Biology 2023Quote: ... The proteins were separated by 12% Tris–Glycine– SDS–PAGE and the gel was electroblotted to a PVDF membrane (0.2 µm) followed by immunodetection using Anti-HaloTag® Monoclonal Antibody (1:5000) (Promega, Madison, Wisconsin, United States) in Tris-buffered saline (TBS ...
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bioRxiv - Cancer Biology 2021Quote: ... The endpoints of the second screen were caspase 3/7 activity measured using the CaspaseGlo® 3/7 assay reagent (Promega) at the 8-hour and 16-hour time intervals ...
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bioRxiv - Neuroscience 2021Quote: Cell cytotoxicity was detected by measuring lactate dehydrogenase (LDH) levels using the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, G1780). The manufacturer’s instructions were followed and the absorbance reflecting the LDH content in the cell supernatant was measured at 492 nm (600 nm reference ...
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bioRxiv - Cancer Biology 2021Quote: ... were seeded in 96-wells white plate and caspase-1 activities were assessed using Caspase-Glo 1 Inflammasome Assay (Promega #G9951) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: Intracellular NanoBRET assays were performed as previously described (10) using NanoLuc®-STK3 Fusion Vector Cat.# NV4301 (Promega, Madison, WI, USA). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... Cell growth and viability were measured on the 96 well white bottom plate as an end point measurement for each time point and/or treatment using the Cell-titer GLO assay (Promega, # G7570) based on manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The caspase-3/7 activity of the lysates or the plated cells was analysed using Apo-ONE ® Homogenous Caspase-3/7 Assay (Promega) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... TUNEL assay was performed using a DeadEnd(tm) Fluorometric TUNEL System according to the manufacturer’s instructions (Promega Corporation, Madison, WI, USA). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... The activities of firefly luciferase (LUC) and Renilla luciferase (REN) were measured using a Dual-Luciferase Reporter Assay System (E2920, Promega, USA). The LUC activity was normalized to the REN activity ...
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bioRxiv - Neuroscience 2020Quote: ... cells were washed with PBS and lysed directly on the plate on ice using the Cell Culture Lysis Reagent from the Luciferase Assay System (Promega#E1500). Lysates were transferred to microcentrifuge tubes ...
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bioRxiv - Developmental Biology 2020Quote: ... and 20 µL of resulting supernatant was used to measure luciferase activity with the Dual-Luciferase Reporter Assay System (Promega, E1980) on a GloMax-Multi plate reader (Promega).
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bioRxiv - Microbiology 2022Quote: ... medium was changed and cellular expression of luciferase reporter indicating viral infection was determined using ONE-Glo™ Luciferase Assay System (Promega) in 48 hr after infection ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cell viability was determined prior to gene expression studies on day 7 by measuring ATP release in supernatants with the CellTiter-Glo 3D assay (Promega, G9681) according to the manufacturer’s protocol to pre-specify appropriate testing ranges ...
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bioRxiv - Neuroscience 2021Quote: ... neonatal and adult astrocytes plated on 96-well plates (50,000 cells per well) was measured with the Glucose Uptake-Glo™ Assay (Promega, J1341) according to manufacturer’s protocol ...
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A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteinsbioRxiv - Molecular Biology 2020Quote: ... The absorbance of cells was measured every 24 hours using CellTiter 96® Non-Radioactive Cell Proliferation Assay (Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 μL of cell lysate was distributed to mix with 25 μL of Nano-Glo Luciferase Assay System (Promega, Cat.# N1120), and another 25 μL of cell lysate was mixed with 25 μL of ONE-Glo Luciferase Assay System (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... and then Renilla luciferase activities were measured after the addition of 100 ml of Stop & Glo® Reagent (Luciferase Assay Reagent, Promega). Finally ...
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bioRxiv - Genomics 2020Quote: ... DNA was subcloned into the enhancer barrier vector28 (gift from Dr. Laura Elnitski, NHGRI) or pGL4.23 for enhancer assays (Promega, Madison, WI). Constructs were prepared using the Plasmid MidiPrep Kit (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... Firefly luciferase activity was measured by adding 25 μL Luciferase Assay Buffer II (LARII) to 5 μL of lysate and measured immediately with the GloMax 20/20 Luminometer (Promega, E5311). Reactions were stopped and Renilla luciferase activity was measured by adding 25 μL Stop&Go reagent ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: PTC readthrough by ACE-tRNA was assessed by measuring NanoLuc (NLuc) luciferase activity using the Nano-Glo Luciferase Assay System (Promega, #N1130) on a Synergy2 multi-mode microplate reader (BioTek Instruments ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates that produced over 104 luminescence units sing a high expression RNA containing NanoLuc Luciferase (A[WT]) and the Promega Nano-Glo Luciferase assay system (Promega N1110) in preliminary tests were pooled and optimized for the needed amount of Mg(OAc)2 and incubation time using A[WT] mRNA ...
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bioRxiv - Molecular Biology 2021Quote: ... The experiments performed here used a pool of lysates from multiple different4 harvests that were each individually tested for a minimal activity of 104 using a high expression RNA containing NanoLuc (A[WT]) and the Promega Nano-Glo Luciferase assay system (Promega N1110). Pooled lysates were then optimized for the needed amount of Mg(OAc)2 and an optimal incubation time at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 8uL of reaction was mixed with 8uL of Nano-Glo buffer/substrate mix following the Nano-Glo Luciferase Assay System (Promega N1110) instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... CA) already containing 20uL of room temperature 1X Passive Lysis Buffer from the Dual Luciferase Reporter Assay System (Promega, Madison, WI). Cultures were then lysed by shaking at 300 rpm for 25 minutes at room temperature ...
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bioRxiv - Genetics 2020Quote: ... Cells were harvested 48 hours after transfection and assayed for luciferase activity using the Dual-Luciferase Reporter (DLR™) Assay System (Promega). Data were expressed relative to renilla luciferase activity to normalize for transfection efficiency ...
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bioRxiv - Biophysics 2021Quote: ... and the twitchin kinase domain lacking flanking tails (Kin) was measured by monitoring ATP hydrolysis using the Kinase Glo™ luminescent assay system (Promega). Assays were performed using 10 µg of protein sample in a 50 µL reaction volume ...
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bioRxiv - Microbiology 2020Quote: ... Plates were incubated at 37°C (5% CO2) for 48 h before performing CellTiter-Glo (CTG) assays as per the manufacturer’s instruction (Promega, Madison, WI). Luminescence was read on a BioTek Synergy HTX plate reader (BioTek Instruments Inc. ...
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bioRxiv - Cancer Biology 2020Quote: ... expression of the luciferase reporter plasmid followed by measurement of luciferase activity after lysing cells with Bright-Glo Luciferase Assay System (Promega #E2610), both using Biotek Cytation 5 plate reader ...
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bioRxiv - Biochemistry 2020Quote: ... Cellular debris was then pelleted by centrifugation and either 5 μl or 10 μl of the clarified supernatant used in dual luciferase assays following the manufacturer’s prescribed protocol (Promega, cat # E1910). Data are shown as the ratio of RLUC to FLUC activity averaged over six experiments.
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bioRxiv - Neuroscience 2020Quote: 50 μl supernatant were collected as designed time point into a 96-well plate for lactate dehydrogenase (LDH) release assay as manufacture’s protocol (Promega, cat# G1780). Briefly ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were collected in lysis buffer and Firefly/Renilla luciferase activity were measured according to the manufacturers’ protocol using the Dual-Luciferase® Reporter Assay System (E1910; Promega) on a Microplate Luminometer LB 96V (EG&G Berthold) ...
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bioRxiv - Cancer Biology 2021Quote: ... Firefly and Renilla luciferase activities were determined 24 hours post transfection with the dual-luciferase reporter assay system (Promega, cat.no. E1910). Firefly luciferase readings were normalized against internal control Renilla luciferase and calculated as fold differences against the activity obtained from cells transfected with empty vector.
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bioRxiv - Cancer Biology 2021Quote: ... treated with LD50 concentrations of 10058-F4 or CB-839 for 12 h in DMEM without FBS then analyzed by Glutamate-Glo™ Assay (Promega) according to the manufacturer’s instructions ...