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Citations for Promega :
5401 - 5450 of 8279 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The cytotoxicity of compounds (100 uM to 1 uM, 3-fold dilution) was examined using the CellTiter-Glo Luminescent Cell Viability Assay (Promega). Cell cytotoxicity data was normalized to DMSO control as 0% cell death.
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bioRxiv - Microbiology 2021Quote: ... Sister plates were exposed to drug but not infected to gauge cytotoxicity via CellTiter-Glo assay (CTG, Promega, Madison, WI), 48 hrs post treatment.
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bioRxiv - Cell Biology 2021Quote: Proteasome activity was measured in iPSC-CMs using the Proteasome-Glo™ Chymotrypsin-Like Assay according to manufacturer’s instructions (Promega). Cells were incubated with the inhibitors (ranging from 2 to 5000 nM ...
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bioRxiv - Immunology 2020Quote: ... the cells were lysed and luciferase activity was measured in the lysates using the Nano-Glo Luciferase Assay System (Promega). Relative luminescence units (RLU ...
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bioRxiv - Pathology 2021Quote: ... induction of apoptosis was determined by measurement of caspase-3/7 activity using the luminometric Caspase-Glo 3/7 assay (Promega) according to the manufacturer’s protocol and a microplate reader (Microplate Reader SH900) ...
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bioRxiv - Molecular Biology 2020Quote: ... Western blots to assess the abundance of the proteins were performed on the same volumes of lysates obtained from the luciferase measurements using the passive lysis buffer supplied with the Dual-Luciferase Reporter Assay System (Promega). Antibodies used for western blots were previously described (Grozdanov et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... Reporter plasmids were constructed by inserting the mouse Olig2 gene mR2 and mR6 regions identified by the ChIP assays in situ into the pGL3-Basic vector (Promega), respectively ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed with passive lysis buffer 48 hours after infection and reporter activity was then measured with a Nano-Glo luciferase assay system (Promega). For IFN-I based experiments increasing amounts of IFN-I were added 16 hours before infection and for TRIM25 overexpression experiments cells were reverse transfected with either TRIM25 or YFP as well as TIM1 24 hours before infection.
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bioRxiv - Cancer Biology 2021Quote: Cells were seeded at a concentration of 3×105/mL in RPMI medium on day 0 and the number of cells was measured every two-three days using the MTS assay from Promega following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... After 24 hours the cells were washed briefly with 1x PBS prior to measurement of luciferase activity by Dual Luciferase Reporter Assay (Promega).
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bioRxiv - Cancer Biology 2020Quote: ... Induction of ISRE-Luc reporter was evaluated by measuring Firefly and Renilla luciferase activity with dual-luciferase reporter assays on Glomax-Multi Detection System (Promega). The results were presented as relative light units (RLU) ...
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bioRxiv - Cancer Biology 2021Quote: The LC50 values for the drugs (MTX, CPT and TDX) were determined by CellTiter-Blue® cell viability assay (Promega). Cells were seeded into 96 plates at a density of 3000 cells per well ...
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bioRxiv - Zoology 2021Quote: ... The cells transfected with different recombinant vectors were cultured for additional 36 h at 37 °C for transcriptional activity analysis by using the Dual-Glo Luciferase Assay System (Promega) with a luminometer (Promega ...
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bioRxiv - Biochemistry 2020Quote: ... the culture medium was aspirated and 20 μL of cell lysate was added from the Luciferase Assay System (Promega, E1500) to each well ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were exposed to the compounds for 72h and then processed for CellTiter-Glo® Luminescent Cell Viability Assay (Promega) readout according to manufacturer’s instructions and read on EndVision (Perkin Elmer) ...
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bioRxiv - Cancer Biology 2020Quote: ... Gene expression in the tumor was analyzed by using human primers using SYBR green gene expression assays (GoTaq qPCR Master Mix, A6002, Promega).
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bioRxiv - Cancer Biology 2020Quote: ... Cell viability for 3 biological replicates of each treatment condition was assessed after 72 hours after drug treatment using the CellTiter-Glo Luminescent Cell Viability Assay (G7571, Promega) and an EnVision Multilabel Plate Reader (PerkinElmer) ...
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bioRxiv - Physiology 2021Quote: ... the firefly luciferase signal of the unk-reporter and the renilla luciferase signal were quantified using the Dual-Glo Luciferase Assay system (Promega) following the manufacturer instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... assessed every day for 4 days by measuring ATP content through Cell Titer-Glo® Luminescent Cell Viability assay (Promega), was compared to cell viability assayed at day 1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysates were prepared by addition of 0.3N hydrochloric acid then 450nM Tris (pH=8) and metabolites were analyzed using the Glutamine/Glutamate Glo Assay (Promega; J8021) per manufacturer’s instruction.
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bioRxiv - Genetics 2020Quote: ... Transfected cells were assayed after 48 h (as indicated for firefly and Renilla luciferase activity using the Dual-Luciferase Reporter Assay System (Promega) in a GloMax® Explorer System (GM3500).
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bioRxiv - Cancer Biology 2021Quote: ... Cell number was measured after 3 and 7 days and normalized to the initial reading at day 0 using the CellTiter Glo Luminescent Cell Viability Assay (Promega). The experiments shown represent fold change at day 7 relative to day 0 ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 ml of culture supernatants were collected every 6 hours and nanoluciferase activities associated with released virus in the supernatants were measured using Nano-Glo luciferase assay system (Promega).
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bioRxiv - Immunology 2020Quote: The fluorescent fusion assay was adapted to allow quantitative measurement of cell-cell fusion by leveraging the NanoBiT complementation system (Promega). Donor HEK293 cells were transfected with full-length S and the 11S subunit in 96-well white flat bottom TC-treated microtest assay plates ...
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bioRxiv - Microbiology 2020Quote: ... IRES activity was determined by measuring Renilla luciferase (RLuc) and firefly luciferase (FLuc) activities using the Dual Luciferase Reporter Assay System (Promega).
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bioRxiv - Microbiology 2020Quote: Cytotoxicity of macrophages after 8 hours of infection was quantified by measuring the release of the enzyme lactate dehydrogenase in cell supernatants using the CytoTox 96 Non-Radioactive Cytotoxicity Assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed in 1 x PBS and cell viability was assessed using CellTiter-Glo Luminescent Cell Viability Assay (Promega). Luminescence read-out was normalized to signal from vehicle treated cells and represented as percentage of viable cells compared to vehicle ...
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bioRxiv - Immunology 2021Quote: Cell death was assessed by measuring lactate dehydrogenase (LDH) release into the supernatant using a CytoTox 96 Non-Radioactive Cytotoxicity Assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 100 mL of media was removed and replaced with 100 mL of Bright-Glo Luciferase Assay Reagent (Promega, Cat # E2620). Luciferase activity was measured on the EnSpire Plate Reader (PerkinElmer) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed and luciferase activity was assessed using the Luciferase Assay System and the Glomax 20/20 luminometer (Promega). Infectivity values were analyzed and plotted using Prism 8.
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bioRxiv - Molecular Biology 2021Quote: Cells were harvested two days after transfection and firefly and Renilla luciferase activities were measured using the Dual-Luciferase reporter assay system (Promega). Total RNA was isolated using TriFast (Peqlab biotechnologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... Luciferase assays require washing the harvested cells 3 times in PBS prior to lysis in passive lysis buffer (Promega, E1941). NLuc activity was assayed using the Nano Luciferase Assay Kit (Promega ...
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bioRxiv - Microbiology 2021Quote: ... The reading of luminescence was performed using the Nano-Glo® Luciferase Assay System according to the manufacturer’s instructions (Promega). After 3 minutes of incubation ...
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bioRxiv - Immunology 2020Quote: ... Recombinant DJ-1 protein (1 μM) was added to the transfected HEK293 cell lines and luciferase activities were measured using a Luciferase Assay System (Promega). 10 μg mL−1 of peptidoglycan (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2020Quote: ... The luciferase assay was performed by thoroughly mixing 10 μl of a sample with 50 μl of the luciferin substrate (Luciferase Assay System, Promega) and measuring the luminescence intensity after 10 and 20 seconds using a luminometer (TD-20/20 Luminometer ...
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bioRxiv - Immunology 2021Quote: ... extensively washed to remove unbound virus and co-cultured with Huh7.5 for 5 days at 37°C after which they were analyzed for with the Luciferase assay system (Promega, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and luciferase activity was quantified in a Tristar LB 941 luminometer (Berthold Technologies, Bad Wildbad, Germany) using Renilla Luciferase Assay System (Promega) as recommended by the manufacturer.
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bioRxiv - Cell Biology 2021Quote: ... Aliquots of culture medium (300 μl) were taken at indicated timepoints and mixed with 100 μL of 4X RealTime-Glo extracellular ATP assay reagent (Promega) reconstituted in RPMI 1640/B27(-Ins ...
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bioRxiv - Immunology 2021Quote: ... caspase-1 activity in the cell culture supernatants was measured using Caspase-Glo® 1 inflammasome assay according to the manufacturer’s instructions (Promega). Luminescence was measured using an Omega plate reader.
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bioRxiv - Cancer Biology 2021Quote: ... cells were lysed in the plate with 250 μl of 1x Passive Lysis Buffer and luciferase activity was determined using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: The viability of cells was measured after six days exposure to various concentrations of drugs using the Cell Titre-Glo assay (Promega). Long-term drug exposure effects were assessed by colony formation assay after 12–14 days exposure to a drug-containing medium (refreshed weekly ...
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bioRxiv - Cancer Biology 2023Quote: ... in 6 well plates or 96 well plates (details in the figure legends) and assayed using crystal violet staining or Celltiter-Glo luminescent viability assay (Promega) according to manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... Cell viability was assessed using a Cell Titer 96 AQueous One Solution Cell Proliferation Assay (Promega Corp, Madison, Wisconsin, USA). Absorbance measurements at 490 nm were normalized to the maximum read per cell line ...
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bioRxiv - Biochemistry 2022Quote: ... Luciferase activity was determined 18-24 hr post-transfection from cell lysate using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturers protocols ...
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bioRxiv - Immunology 2023Quote: ... 105 cells per target population were sorted and kept on ice until further processing using GSH-GloTM Glutathione Assay (Promega), strictly following manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... The plates were incubated at 37°C for ~12 hours before luciferase activity was quantified using the Luciferase Assay System (Promega) using SoftMax Pro v.7.0.2 (Molecular Devices) ...
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bioRxiv - Immunology 2023Quote: ... and luciferase activity was measured in a Tecan infinite 200Pro by automatic injection of 50 μl luciferase assay substrate (Promega) and 10 sec integration time.
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bioRxiv - Plant Biology 2022Quote: ... The activity of Firefly luciferase and the reference Renilla luciferase were quantified using the Dual-Luciferase Reporter Assay System (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: The bioluminescence resonance energy transfer (BRET) ubiquitination assay was performed using the NanoBRET Nano-Glo Detection Systems (Standard) (N1661, Promega) according to the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2023Quote: ... Luminescence was measured for each N1-X/N2-Y pairwise combination by auto-injecting 50 µL Renilla luciferase substrate (Renilla Luciferase Assay system, catalog No. E2820, Promega) per well and integrating light output for 4 s using a TriStar luminometer (Berthold) ...