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Citations for BioLegend :
6301 - 6350 of 7138 citations for Primary Human Melanocyte Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... target cells were identified by flow cytometric surface staining with CD38-PE (Fisher) and CD138-PERCP-Cy5.5 (Biolegend) (DL-101 ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated for 10 min at 4 °C with Fc receptor block (TruStain FcX, BioLegend, 1:10). Cells were washed and incubated in FACS buffer (1xPBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Then blocking solution was aspirated and the cells were incubated with phycoerythrin (PE) anti-FOLR1 antibody (908304, Biolegend) at room temperature for 1h ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T cells were >99% pure and assessed by flow cytometry staining with CD8-Pacific Blue antibody (Biolegend). Total CD8+ T cells were activated using anti-CD3/CD28 coated beads (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Activation of CD8+ CAR-T cells was determined with CD25 staining using CD25-APC antibody (Biolegend, cat# 302610). CAR expression of anti-CD19 CAR was determined with Human CD19 (20-291 ...
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bioRxiv - Immunology 2024Quote: ... Cells washed with 500 µL of 1% FBS in PBS and incubated with streptavidin-AF488 (1:200, BioLegend) for 20 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... cells were washed once more with PBS and stained with the Zombie VioletTM Fixable Viability Kit (Biolegend, USA) for 15 min following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were incubated for 30min with the following antibodies in 1:100 concentration: APC anti-mouse CD45 (Biolegend), APC anti-mouse Ter119 (Biolegend) ...
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bioRxiv - Cancer Biology 2024Quote: ... then the cell pellet was resuspended in 100µL PBS with 1µL Zombie Green viability dye (Biolegend, cat# 423111). Cells were stained at room temperature out of light for 15 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... This was centrifuged again (300xg for 7 minutes) and resuspended in 1X red blood cell lysis buffer (BioLegend) in deionized water ...
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bioRxiv - Immunology 2024Quote: ... Naïve T cells were skewed to the following subsets with cytokines: Th1 (10ng/mL mIL-12 (Biolegend, 577004), 10ng/mL recombinant human IL-2 ...
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bioRxiv - Bioengineering 2024Quote: ... and cell pellets were washed with PBS before being treated with the Zombie NIRTM Fixable Viability Kit (Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Activated total PBMCs or isolated activated CD4+ and CD8+ T cells were treated with anti-PD-1 (BioLegend, Ultra LEAF purified human anti-PD-1 antibody ...
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bioRxiv - Neuroscience 2024Quote: ... Brain and spleen cells were stained with 1 µL from each FITC anti-mouse CD45 (Biolegend, Cat. 157214), PE anti-mouse CD11b (Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: ... then the cell pellet was resuspended in 100µL PBS with 1µL Zombie Green viability dye (Biolegend, cat# 423111). Cells were stained at room temperature out of light for 15 minutes ...
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bioRxiv - Immunology 2024Quote: ... Red blood cells were then removed with a 2-minute incubation in RBC Lysis Buffer (10X) (BioLegend 420301). The solution was centrifuged again at 300 x g ...
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bioRxiv - Immunology 2024Quote: ... Single cell suspensions were stained with the Zombie NIR™ Fixable Viability KIT (780nm of excitation; Biolegend, #423106) at a 1/250 dilution in PBS (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated for 15 min at room temperature in 100 μl of Annexin binding buffer (ABB, BioLegend) containing APC-labeled Annexin V ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells from the remaining wells were collected and resuspended in 1mL PBS with Zombie Violet™ 1000x (BioLegend®) to exclude dead cells and analyzed by flow cytometry ...
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bioRxiv - Cell Biology 2021Quote: Detection of apoptotic cells was done by using FITC-Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend#640922). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... Following two washes with PBS (centrifuged at 700 x g for 5 minutes), cells were stained in 200 μL PI staining solution (0.1% Triton X-100, 50 μg propidium iodide (BioLegend), 0.2 mg/mL RNase ...
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bioRxiv - Immunology 2021Quote: ... mice were irradiated with a total of 1100 rad in two divided doses and injected intravenously with a 1:1 mixture of T cell-depleted (magnetic bead depletion; Thy1.2; BioLegend) bone marrow from CD45.1+CD45.2+ mice and control C57BL/6J (CD45.2+ ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained for 20-30 minutes on ice with 1:200 anti-CD11b-BV711 (Clone M1/70; Biolegend) prior to analysis by flow cytometry ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated with Live/Dead Yellow (1:1000) followed by Fc block (1:200; BioLegend, San Diego, CA), and stained with antibody cocktails for 30 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... were co-incubated with 6×105 target cells expressing mCherry at 37°C for 2 hours in the presence of 2µM monensin (BioLegend). The cells were centrifuged ...
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bioRxiv - Immunology 2021Quote: ... the block was washed off and the cells were stained with 50μl of Anti-Mouse anti-CD-11b-BV785 (clone M1/70, Biolegend) and anti-F4/80-BV650 (clone BM8 ...
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bioRxiv - Immunology 2021Quote: ... lymph node cell IL-1β/IL-23 plus spermidine treatment group (LN sper) with hashtag 5 (BioLegend, Cat# 155809). The five cell populations were mixed and barcoded ...
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bioRxiv - Immunology 2021Quote: ... spleen T cell IL-1β/IL-23 plus spermidine treatment group (SPT sper) with hashtag 3 (BioLegend, Cat# 155805); lymph node cell IL-1β/IL-23 stimulation group (LN stim ...
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bioRxiv - Developmental Biology 2021Quote: ... E14 cells were collected from culture dishes and incubated for 30 min at 4 °C with CITE-seq cell hashing62 antibody Ab_CD15 (1:200) (Biolegend). XEN-eGFP were collected from culture plates and incubated for 30 min at 4 °C with CITE-seq cell hashing antibody Ab_CD140 (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... XEN-eGFP were collected from culture plates and incubated for 30 min at 4 °C with CITE-seq cell hashing antibody Ab_CD140 (1:200) (Biolegend). In the gastruloid sample ...
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bioRxiv - Biochemistry 2020Quote: ... Purity of isolated cells was checked by flow cytometry using antibodies against neutrophil surface markers: CD11b (PE) (Biolegend, 301305) and CD15 (AlexFluor700 ...
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bioRxiv - Immunology 2021Quote: ... then stained with the I-A/E-APC antibody (MHCII) (1:1,200) (eBioscience) and positively selected for I-A/E-positive cells using anti-APC Microbeads (Biolegend). The obtained final cell fraction consisted of total mTECs (CD45-EpCAM+Ly51-I-A/E+).
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bioRxiv - Immunology 2021Quote: ... The depleted cell fraction was then stained for 20 min at 4 °C with the CD45-PerCPCy5.5 antibody (1:50) (Biolegend) and the EpCAM-FITC antibody (G8.8 clone ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were incubated for 1 h on ice in PE/Cy7 anti-mouse CD45.2 antibody (clone:104, BioLegend) and PE-conjugated anti-mouse IL1a antibody (clone:ALF161 ...
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bioRxiv - Molecular Biology 2021Quote: CH12 cells were stimulated to undergo CSR to IgA by treatment with 1-5 μg/mL α CD40 (BioLegend), 5 ng/ml TGF (R&D Systems ...
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bioRxiv - Immunology 2020Quote: ... were added to the plated tumor cells and co-cultured overnight in the presence or absence of anti-PD-L1 2μg (BioLegend). Following overnight incubation ...
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bioRxiv - Microbiology 2022Quote: ... The cells were washed twice and resuspended in 300 μl of staining buffer containing 7-AAD (Biolegend, CA, USA) for flow cytometry analysis (Thermo Fisher Attune NxT - 14 color analyzer ...
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bioRxiv - Physiology 2022Quote: ... Cells were then washed in HBSS with 3% BSA and incubated with CD34 Brilliant Violet 421 (BioLegend 230 119321) at 1:400 and CD24 PerCP-Cyanine 5.5 (eBioscience ...
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bioRxiv - Microbiology 2022Quote: ... the cell culture media was replaced with complete Huh-7.5 media with 5 μg/mL BFA (Brefeldin A, Biolegend) or equal volumes of DMSO (vehicle control ...
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bioRxiv - Microbiology 2022Quote: ... The followed anti-mouse antibody panels were used for cell subset analysis: CD45 (clone 30-F11, Pe-Cy7, Biolegend), CD3e (clone 145-2C11 ...
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bioRxiv - Genomics 2019Quote: ... The resuspended cells were incubated for 20 min at 4 °C with the fluorophore-labeled antibodies CD45-PerCPCy5.5 (1:50) (Biolegend), Ly51-PE (1:800 ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated at 4°C for 15 minutes with an Fc receptor blocker (BioLegend TruStain FcX, cat # 422302) according to manufacturer instructions before cocktails of surface antibodies were added for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Mouse neutrophils were purified from bone marrow cells using negative magnetic bead selection according to the manufacturer’s instructions (MojoSort, BioLegend). These neutrophils had > 90% purity and > 90% viability as determined by flow cytometry ...
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bioRxiv - Immunology 2021Quote: ... and stained with 1 ug/test cell-hashing antibody (TotalSeq C0251, C0254, C0256, C0260, clones LNH-95, 2M2, BioLegend) for 30 minutes at 4C ...
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bioRxiv - Immunology 2021Quote: ... about 4 × 106 cells were stained with 1 μl mouse Fc-block (anti-CD16/32; BioLegend, San Diego, CA) in 50 μl FACS buffer (PBS containing 2% FBS ...
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bioRxiv - Bioengineering 2019Quote: ... the cell morphological change was examined under bright field microscope and macrophage markers CD36 (2.5 µg per 1×106 cells in 100 µL; Cat. no. 108418, BioLegend) and CD71 (2.5 µg per 1×106 cells in 100 µL ...
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bioRxiv - Cancer Biology 2019Quote: ... Purified cells were plated at 1×105 cells per well on a 96 well plate coated with 10μg/ml αCD3 (Biolegend), and cultured with 2μg/ml soluble αCD28 (Biolegend) ...
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bioRxiv - Cell Biology 2019Quote: The immune phenotype of isolated cells was analyzed by staining with monoclonal antibodies CD90-PE/Cy5 (328112; BioLegend, USA), CD90- Brilliant Violet 421 (328122 ...
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bioRxiv - Immunology 2019Quote: ... Total cells were counted by hemacytometer and incubated with PE-conjugated-anti-CD3 (Clone 17A2, BioLegend, San Diego, CA) at a concentration of 1 µg / 106 cells for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were washed three times in FACS buffer and resuspended in FACS buffer containing 3 μg/ml DAPI (BioLegend). Flow cytometric analysis was done on a BD LSRFortessa analyzer and sorting was done on a BD FACSAria1 machine.