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Citations for BioLegend :
6451 - 6500 of 7138 citations for Primary Human Melanocyte Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Cells were then incubated with the L5 clone of Alexa Fluor® 594 anti-Flag antibody (BIOLEGEND INC, 637314), diluted 1:200 in PBS buffer containing 2% BSA ...
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bioRxiv - Immunology 2022Quote: ... enriched single-cell suspensions from tumor lysates were labelled with biotinylated anti-CD11b antibody (BioLegend, San Diego, CA, USA) and separated on MACS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were first stained with viability stain (Zombie NIR fixable viability kit, BioLegend, San Diego, California, USA) followed by either BV510-αCD45 (clone ...
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bioRxiv - Bioengineering 2023Quote: Lung single-cell suspensions were stained for 30 min at RT with Zombie Red™ Fixable Viability Kit (BioLegend) and were blocked with anti-mouse CD16/32 (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with PBS and intracellularly stained with PE anti-phospho-p38-MAPK (Thr180/Tyr182; 1:20; BioLegend) ab or PE mouse isotype control IgG1,κ (1:20 ...
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bioRxiv - Immunology 2023Quote: ... and then single-cell suspensions were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Biolegend, #426803) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... At the end of the incubation cells were stained with PE anti-CD8 antibody (clone RPA-T8, Biolegend #301008) and the degranulation extent was measured among the GFP+ population as the percentage of CD8+ T cells expressing LAMP1 on their surface by flow cytometry analysis.
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bioRxiv - Bioengineering 2023Quote: ... The following antibodies were mixed together and added directly to the cell suspension: BV605 CD45 (1:500, Biolegend, 103139), AF700 CD11b (1:300 ...
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bioRxiv - Cancer Biology 2023Quote: ... naïve CD4+ T cells were co-cultured with APCs at indicated ratio in the presence of 0.5 μg/mL (anti-CD3 BioLegend), 0.5ng/mL TGFβ (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... expanded CD4+ T cells were cocultured at different ratios with CFSE/CTV labeled CD8+ T cells and stimulated with Mouse T-Activator CD3/CD28 Dynabeads at 2 cells per bead ratio and 0.5ng/mL IL-2 (BioLegend). Cell culture media consisted with RPMI 1640 ...
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bioRxiv - Neuroscience 2023Quote: ... Single cell suspensions were first stained for viability using Zombie NIR™ Fixable Viability Kit (BioLegend; San Diego, CA) for 20 min at room temperature in phosphate buffer solution pH 7.2 (PBS ...
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bioRxiv - Immunology 2023Quote: ... A single cell suspension was generated and stained with eBioscience’s Fixable Viability Dye eFluor780 and anti-CD45.2 (104, BioLegend, #109808).
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bioRxiv - Immunology 2023Quote: ... followed by 100ul of Streptavidin nanobeads for 1×10^8 cells in 1ml of MACS buffer for 15min on ice (MojoSort Mouse pan B cell isolation kit II, Cat.no. 480088, Biolegend). The cell suspension was diluted and put on a pre-rinsed LS Column (Cat ...
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bioRxiv - Immunology 2023Quote: ... cells were stimulated with PMA/ionomycin with Brefeldin A (1:500 or equivalent to 1.3 μM ionomycin and 81 nM PMA, Cell Activation Cocktail, Biolegend) for 4 hours ...
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bioRxiv - Immunology 2023Quote: ... Cells were then incubated with FITC, and PE-conjugated antibodies (1:100, IFNr or FoxP3, GZMB) purchased from Biolegend. Aqua LIVE/DEAD used for cell viability was purchased from Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... The cells were then stained with Alexa Fluor® 700 anti-mouse CD206 (MMR) antibody (1:200; Biolegend, C068C2) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... cell pellet was resuspended in 100 µl ice cold PBS and 0.2 µL Zombie NIR viability dye (BioLegend, 77184). Cells were kept on ice until their flow cytometry analysis using FACS Canto II from BD-Biosciences.
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bioRxiv - Immunology 2023Quote: ... Cells were incubated 20 min on ice with anti-CD16/32 (Fc-block, clone 93, BioLegend, San Diego, CA) then stained with the following antibodies directed toward cell surface receptors ...
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bioRxiv - Immunology 2023Quote: ... Isolated lymphocytes were stimulated with PMA/ionomycin with Brefeldin A (1:500 or equivalent to 1.3 μM Ionomycin and 81 nM PMA, Cell Activation Cocktail, Biolegend) for 5 h at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... Quality and purity of isolated cells was estimated by flow cytometry after surface staining for CD3 (Biolegend, Cat.No. 300428), and γ9 TCR (Biolegend ...
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bioRxiv - Genomics 2023Quote: ... Cells were stained on ice in the dark for 30 mins with CD34-APC (clone 581; Biolegend; 1:100), CD38-PE/Cy7 (clone HB7 ...
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bioRxiv - Cell Biology 2023Quote: ... the cell pellet was resuspended in 1% BSA/ PBS containing secondary fluorescence antibody (PE anti-mouse IgG1, BioLegend, 406608) and incubated for 1 h as described above ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were labelled with rat-anti-Prominin-1 antibody (Brilliant Violet 421-conjugated; BioLegend, #141213, Lot# B255870, 1:1000) and Alexa Fluor 647-conjugated recombinant human EGF (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... the cells were harvested and washed twice before subjecting to propidium iodide (PI) solution (10 µg/ml) (BioLegend, #421302) for 10 min ...
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bioRxiv - Immunology 2023Quote: Antibodies used for staining sections for confocal imaging or isolated cells for flow cytometry include: CD64 (clone X54-57.1; BioLegend), B220 (clone RA3-6B2 ...
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bioRxiv - Immunology 2023Quote: ... A single cells suspension was generated and stained with eBioscience’s Fixable Viability Dye eFluor780 and anti-CD90.2 (30-H12, BioLegend, #105308) and anti-CD19 (6D5 ...
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bioRxiv - Systems Biology 2022Quote: Sorted live single cells were centrifuged at 400 x g for 5min at 4°C and resuspended in 25μL of cell staining buffer (BioLegend). Human TruStain FcX FC blocking reagent (BioLegend ...
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bioRxiv - Systems Biology 2022Quote: ... Each tube was made up to 100μL with cell staining buffer and TotalSeq Hashtag (HTO 1-8) reagents (BioLegend) were added to each sample for 20min on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were also supplemented with a physiological growth factor cocktail consisting of 0.2 ng/ml stem cell factor (SCF, catalogue no. 573902; BioLegend), granulocyte macrophage colony-stimulating factor (GM-CSF ...
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bioRxiv - Cancer Biology 2022Quote: ... about 1-2e6 mononuclear cell suspension was stained with a panel of Total-seq B antibodies (1ug/each, Biolegend) and fluorochrome conjugated flow antibodies for CD45 (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... Cell mixtures were then washed in PBS and stained with mAbs to CD56 (FITC or BV605, Biolegend, #318304, #318334), NKG2A (PE ...
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bioRxiv - Immunology 2023Quote: Listed below are the antibodies used for flow cytometric analysis of mouse cell-surface molecules: ① Purchased from Biolegend(USA): Anti-CD45-BV510(clone 30-F11);Anti-Ly-6G/Ly-6c(Gr-1)-PerCP(clone RB6-8C5);Anti-CD4-BV421(clone GK1.5);Anti-F4/80-FITC(clone BM8);Anti-CD8a-PE(clone 53-6.7) ...
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bioRxiv - Immunology 2023Quote: ... Soluble peptide:IAg7 proteins were produced using S2 insect cells as previously described and made into tetramers by conjugating the soluble peptide:IAg7 molecules with BV421 (BioLegend), PE ...
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Induction of PARP7 Creates a Vulnerability for Growth Inhibition by RBN2397 in Prostate Cancer CellsbioRxiv - Cancer Biology 2023Quote: ... Cells were then filtered through 35 μm nylon mesh and stained with Annexin V-FITC and 7-AAD (Biolegend). 50,000 cells per sample were collected for analysis using a LSRII flow cytometer (BD Biosciences ...
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bioRxiv - Bioengineering 2023Quote: Single cell suspensions of MSCs were stained for the following antibodies: CD34(MOPC-173) (BioLegend, San Diego, CA, USA), CD146(P1H12 ...
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bioRxiv - Immunology 2023Quote: ... apoptosis of the Neuro-2a cell was analyzed by Annexin-V-FITC and 7-AAD staining accordingly to the manufacturer’s instruction (BioLegend). Cells were immediately acquired on a Canto II flow cytometer (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... and around 1×106 cells were blocked with purified mouse CD16/32 antibody (1:100 dilution; cat. #101301; BioLegend) for 5 minutes on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting single cells were resuspended in PBS and stained with 1:1000 Zombie Violet™ dye (Biolegend, 423113) for 15 minutes to exclude dead cells ...
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bioRxiv - Cell Biology 2022Quote: ... one million cells were first resuspended in 100 μL buffer containing 5 μL Fc Receptor Blocking Solution (Biolegend, #422302) and incubated for 15 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... cells were subject to surface staining by being incubated with a cocktail of anti-CD11b (Biolegend, USA, Catalog #101225), anti-CD45 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... congenic CD45.1 mouse spleen was isolated and red blood cells were lysed with RBC lysis buffer (BioLegend, CA, USA). Leukocytes were then resuspended in complete RPMI with 1µg/mL high molecular weight Poly(I:C) ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated with phospho-EZH2 (S21) (Affinity Biosciences, Cat# AF3822) or rabbit IgG isotype control (BioLegend, Cat# 910801) followed by PE-conjugated anti-rabbit antibody (BioLegend ...
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bioRxiv - Synthetic Biology 2024Quote: ... 50,000 CAR-T cells were co-cultured with 100,000 target cells for 5h in the presence of CD107a antibody (Supp. Table 3) and 1x Brefeldin A (Biolegend). Following co-culture ...
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bioRxiv - Immunology 2023Quote: ... CD4+ OT-II KN2 cells were purified from CD45.1.2 OT-II KN2 donor mice with the MojoSort™ Mouse CD4 T Cell Isolation Kit (Biolegend) and labeled with cell proliferation dye eFluor 450 (0.01 mM ...
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bioRxiv - Immunology 2023Quote: ... Primary mouse lung fibroblasts were freshly isolated after magnetic bead-based negative selection of epithelial cells (118204, Biotin anti-mouse CD326 Epcam, Clone: G8.8, BioLegend), endothelial cells (102404 ...
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bioRxiv - Immunology 2023Quote: ... CD4 T cells were purified from spleens and lymph nodes using MojoSort™ CD4 isolation kit and magnet (Biolegend). Purities of cells were determined for each experiment using flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... Spleen standard cell suspensions were stained in the same conditions but used anti-CD45-FITC (Biolegend 109806, clone 104) to distinguish it from the other samples once pooled ...
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bioRxiv - Immunology 2023Quote: ... cells were washed with cold FACS buffer and fixed in Fixation buffer (Cat#420801, BioLegend, San Diego, CA, USA) at 4 °C for 1 h ...
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bioRxiv - Immunology 2023Quote: ... CD73- CD4+ CD25- Foxp3- newly generated CD4SP thymocytes were sorted from Foxp3eGFP-Cre-ERT2 reporter mice and reconstituted at a concentration of 20,000 cells/100uL in complete RPMI + 100ng/mL recombinant mouse IL-7 (Biolegend). CD4SP thymocytes and DC subsets were co-cultured at a 2:1 ratio in round-bottom 96-well plates for 4 days and then analyzed by flow cytometry on day 5 to evaluate the percentage of Tregs generated in vitro.
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bioRxiv - Immunology 2023Quote: Single-cell suspensions from the tumors and draining lymph nodes were first stained with 1 μL Zombie Aqua (BioLegend) to exclude dead cells ...