Labshake search
Citations for BioLegend :
6201 - 6250 of 7138 citations for Primary Human Melanocyte Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... 106 cells per sample were blocked with 0.025mg anti-CD16/32 (clone 93; BioLegend, San Diego, CA, USA) and then stained with a panel of antibodies shown in Table 1 ...
-
bioRxiv - Immunology 2020Quote: ... Cell death was analysed 18h later by FITC Annexin V/PI Apoptosis Detection Kit with PI (640914; Biolegend).
-
bioRxiv - Immunology 2021Quote: ... CD45+ cells were then positively selected using CD45 antibodies conjugated to magnetic beads (Biolegend MojoSort CD45 nanobeads 480029). Viable cells were counted on a hemocytometer ...
-
bioRxiv - Immunology 2021Quote: ... the isolated B cells were incubated with 1 μl Zombie UV Fixable Viability kit (Biolegend, cat. no. 423107) per 1 ml cell suspension for 30 min on ice ...
-
bioRxiv - Cell Biology 2022Quote: ... dissociated pancreatic cells/units were incubated with anti-mouse CD16/32 (10 μg/mL; BioLegend, San Diego, CA) for 5 min on ice to reduce non-specific binding ...
-
bioRxiv - Cancer Biology 2022Quote: ... The cell suspension was stained with Zombie NIR Fixable Viability Dye or Zombie Aqua Fixable Viability Dye (BioLegend) to label dead cells ...
-
bioRxiv - Immunology 2022Quote: ... Cells were then incubated in 2% FCS/PBS solution containing anti-mouse CD16/32 antibody (BioLegend clone 93) for 15 minutes and subsequently stained with a combination of fluorophore-conjugated primary antibodies against mouse CD45 (BioLegend clone 30-F11) ...
-
bioRxiv - Immunology 2022Quote: ... and bone marrow single-cell suspensions was performed by flow cytometry using fluorochrome-conjugated antibodies purchased from BioLegend, unless stated otherwise ...
-
bioRxiv - Genomics 2022Quote: Single-cell RNA sequencing (scRNAseq) libraries were prepared from viably revived BM samples using hashtag B antibodies (Biolegend) and Chromium single cell 3’V3 reagent kits (10x genomics) ...
-
bioRxiv - Immunology 2022Quote: ... Depleted cells were pelleted and stained with anti-CD34 FITC and anti-FITC biotin (Biolegend, clone FIT-22). Cells were washed ...
-
bioRxiv - Immunology 2022Quote: ... The cells were then incubated for 10 minutes with purified rat anti-mouse CD16/CD32 (Biolegend, 93 clone)(only for mouse cells ...
-
bioRxiv - Genetics 2022Quote: ... PT3 bone marrow-derived CD34+ cells were stained using 1:40 APC-conjugated CD34 (clone 581, 343509, BioLegend). For mtscATAC-seq and ASAP-seq experiments residual granulocytes were excluded by staining of cells using 1:50 PE-conjugated CD66b (clone G10F5 ...
-
bioRxiv - Immunology 2022Quote: ... Heart CD45+ cells were enriched via positive selection using 0.2μg anti-mouse CD45 PE (Biolegend, clone 30-F11) per 1×106 cells followed by anti-PE microbeads (Biolegend) ...
-
bioRxiv - Immunology 2022Quote: ... Single-cell suspensions of rested PBMCs were aliquoted into plates pre-coated with anti-CD3 (clone : UCHT1, Biolegend) and anti-CD28 (Clone:CD28.2 ...
-
bioRxiv - Microbiology 2022Quote: ... the single-cell suspension was stained with the following antibodies: PacBlue-conjugated anti-CD45 (Clone 30-F11, BioLegend) and APC-conjugated anti-EpCAM (Clone G8.8 ...
-
bioRxiv - Microbiology 2022Quote: ... 107 cells per sample were then incubated with 10 μl of Fc-blocker (anti-CD16/CD32 antibody, Biolegend) for 30 min ...
-
bioRxiv - Systems Biology 2023Quote: ... Dead cells were stained with Fixable Viability Dye eFluor 780 (eBioscience) or Zombie Violet Fixable Viability Kit (BioLegend). Cells were stained with the following fluorochrome-conjugated anti-human Abs ...
-
bioRxiv - Immunology 2022Quote: ... 3-4 × 106 splenocytes after cell surface staining were fixed with Cyto-Fast Fix/Perm buffer (BioLegend, USA) for 20 min at room temperature and washed twice with FACS staining buffer ...
-
bioRxiv - Immunology 2022Quote: ... Cell surface staining was performed at RT for 15 minutes with a panel of antibodies purchased from Biolegend, BD Biosciences or Miltenyi (Table S13) ...
-
bioRxiv - Immunology 2024Quote: ... all cells were blocked with 1:50 dilution of TruStain FcX anti-mouse CD16/CD32 (Clone 93, Biolegend) and Fixable Viability Dye (eBioscience ...
-
bioRxiv - Cancer Biology 2022Quote: ... Washed and filtered single-cell suspension were stained with viability dye (Zombie NIR, 1:500, BioLegend, no. 423105), blocked with Fc-block (1:200 ...
-
bioRxiv - Cell Biology 2024Quote: ... single cells were digested from skin wounds and pre-incubated with purified anti-CD16/CD32 antibody (101301, BioLegend) (1.0 μg per 106 cells in 100 μl volume ...
-
bioRxiv - Cancer Biology 2024Quote: ... For senescence and exhaustion NK cell evaluation the following antibodies were used: anti-PD-1 (clone EH12.2H7, Biolegend), - CD57 (clone NK-1 ...
-
bioRxiv - Immunology 2023Quote: ... mLN single-cell suspensions were stained for flow cytometry using PerCP-Cy5.5-conjugated anti-CD80 (16-10A1) (BioLegend), eFluor 450-conjugated anti-CD11c (N418 ...
-
bioRxiv - Cancer Biology 2024Quote: ... anti-PD-1 (Bio X Cell, RMP1-14, BE0146, 10 μg/mL or Biolegend, 114102, 10 μg/mL), anti-CD38 (Novus ...
-
bioRxiv - Neuroscience 2024Quote: Cell suspensions were first stained for viability using Zombie NIR™ Fixable Viability Kit (BioLegend; San Diego, CA) for 20 min at room temperature in phosphate buffer solution (pH 7.2S ...
-
bioRxiv - Immunology 2024Quote: ... 106 cells per sample were blocked with 0.025mg anti-CD16/32 (clone 93; BioLegend, San Diego, CA, USA) and then stained with a panel of antibodies shown in SI Table 9 ...
-
bioRxiv - Immunology 2024Quote: ... Cell supernatant was collected after 24 h to measure IL-6 and TNF-α levels using ELISA (BioLegend) according to manufacturer’s instructions.
-
bioRxiv - Immunology 2024Quote: ... and adjusted to 1 × 106 cells in 1 mL of DPBS containing 10 µM of Calcein-AM (BioLegend) for cell staining at 37°C for 30 min ...
-
bioRxiv - Immunology 2024Quote: ... Cells were stained at room temperature with a viability dye (Zombie NIRTM, Biolegend #423105 or ZombieRedTM, Biolegend #423109) for 15 minutes then washed and stained for 20 minutes at room temperature with fluorochrome-conjugated antibodies ...
-
bioRxiv - Immunology 2024Quote: ... Single cell suspensions were washed in PBS and then incubated with 50 μl Zombie Aqua viability dye (BioLegend) for 10 minutes at room temperature in the dark ...
-
bioRxiv - Immunology 2024Quote: ... Cells were then washed and surface stained with CD4-PE-Cy5 (Clone RPA-T4, BioLegend 300510, 1:400), CD69-BUV496 (Clone FN50 ...
-
bioRxiv - Immunology 2024Quote: Single cell suspensions were stained with Live/Dead stain (LIVE/DEAD Fixable Near-IR, Yellow or Green, Biolegend) and Fc-Receptor block was performed (clone 93 ...
-
bioRxiv - Immunology 2024Quote: ... mast cells were stained using CD117 (c-Kit)-APC or APC/Cy7 and FcεRIα-PE (all from Biolegend) at 1:200 dilution for 20 min on ice ...
-
bioRxiv - Immunology 2024Quote: ... Mouse and human T cells were washed three times with RPMI 10% FBS and 2.5 x 105 cells were added to each well with 2 µg/ml soluble anti-CD28 (37.51 or CD28.2, Biolegend). For co-culture experiments ...
-
bioRxiv - Immunology 2023Quote: Cells were first washed with PBS then resuspended in Zombie Aqua Viability Dye (1:500 in PBS, Biolegend). For extracellular staining ...
-
bioRxiv - Immunology 2023Quote: ... Fc receptors were then blocked by incubating cells in TruStain Fcx plus Fc block (1:100; BioLegend, 156604) in FACS buffer (DPBS containing 2% FBS and 2mM EDTA ...
-
bioRxiv - Immunology 2023Quote: ... cells were re-suspended in PBS and treated with anti-CD16/CD32 (Clone 93, Biolegend, 101301, 1:200) for 15 min on ice ...
-
bioRxiv - Immunology 2023Quote: ... cells were centrifuged and resuspended in 1 ml of −20°C cold TruePhos™ Perm Buffer (BioLegend, USA) and incubated at −20°C for 1 hour ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... The cell suspension was stained with a panel of antibodies against CD45-Alexa 700 (1:1200; Biolegend; #147716) and CD11b-PE dazzle (1:800 ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Dead cells were excluded using the live/dead stain Zombie NIR fixable viability dye (1:2000; Biolegend; #423105). The samples were run on a Cytek® Northern Lights™ cytometer (Cytek biosciences ...
-
bioRxiv - Immunology 2022Quote: ... naïve CD4 T cells were magnetically isolated from the spleen and LNs of Rag2-/- TCR7B8 Tg or TCR1A2 Tg mice on CD45.2/CD45.2 background using the MojoSort™ Mouse CD4 Naïve T cell Isolation kit (BioLegend). Then ...
-
bioRxiv - Molecular Biology 2023Quote: ... Washed cells were stained with two fluorescently labeled epitope tag-specific antibodies (anti-FLAG PE, Biolegend, cat# 637309), anti-HA Alexa 647 ...
-
bioRxiv - Microbiology 2022Quote: ... Cell viability was determined using Zombie-NIR Fixable Viability Dye (0.4 μL per 50 μL staining volume; Biolegend). Antibodies used for surface staining were as follows ...
-
bioRxiv - Immunology 2023Quote: ... cells were treated with 81 nM phorbol 12-myristate 13-acetate (PMA) plus 1.34 µM Ionomycin (BioLegend, 423301) for 30 min ...
-
bioRxiv - Immunology 2023Quote: ... CD45-labelled cells were mixed (maximum 6 samples together) and stained with APC-conjugated FCRL5 antibodies (Biolegend #340305)(for panel #2) ...
-
bioRxiv - Cell Biology 2022Quote: ... pelleted cells were resuspended in MACS buffer containing anti-CD45-FITC (1:1000, clone 30-F11, Biolegend, 103108), PI (1:100 ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were stained with cell surface marker antibodies (CD3ε: PerCP/Cyanine5.5, clone 145-2C11; CD8a: FITC, clone 53-6.7; both BioLegend) and Fixable Viability Dye eFluor 780 (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were kept on ice after detachment and stained for 30 min with anti-ICAM1-PE (Biolegend #353106) and anti-MHCII-FITC (ImmunoTools #21629233X2) ...
-
bioRxiv - Cell Biology 2023Quote: ... magnetic activated cell sorting (MACS) was applied using the mouse IgM BG-1 antibody (BioLegend, San Diego, CA) coupled to α-mouse IgM microbeads (Miltenyi Biotec ...