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Citations for BioLegend :
6151 - 6200 of 7138 citations for Primary Human Melanocyte Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Supernatants were aspirated and cells were then treated with Fc block (clone 93, Ref: 101302, BioLegend, 1:1000) at 4°C for 15 min ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were trypsinized and fixed in 70% ethanol followed by staining with propidium iodide (PI) (BioLegend, Cat # 421301) using manufacture’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... cells in each condition were recovered by trypsin and stained intracellularly with Granzyme B (Biolegend, AF647, clone GB11). Flow cytometry was performed by BD LSR Fortessa HTS and data were analysed using GraphPad Prism V9.
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed twice and each experimental group (ummodified, modified, unpulsed) was differentially stained with either CFSE (Biolegend), CellTracker Deep Red (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed 3 times in FACS buffer and incubated in Cyto-Fast Fix/Perm Buffer (BioLegend, 426803) for 20 minutes at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... CD8 T cells were >99% pure and assessed by flow cytometry staining with CD8-Pacific Blue antibody (Biolegend). Total CD8 T cells were activated using anti-CD3/CD28 coated beads (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... 5×105-1×106 cells were stained with antibodies to CD45 (D058-1283, Biolegend, San Diego, CSA, USA), CD3 (clone SP34 ...
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bioRxiv - Cell Biology 2022Quote: ... Bone marrow cells were differentiated into BMDMs using 40 ng/mL recombinant M-CSF (BioLegend, catalog number 576406) in the same media formulation as TR-AMs ...
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bioRxiv - Microbiology 2022Quote: Single-cell suspensions were incubated and labeled with the following antibodies obtained from BioLegend (San Diego, CA, USA) at a 1:100 dilution ...
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bioRxiv - Cancer Biology 2022Quote: RAW 264.7 cells or BMDMs were cultured as described above and then treated with recombinant mouse TGFβ1 (Biolegend). Fifteen minutes later ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated in 2% FCS/PBS solution containing anti-mouse CD16/32 antibody (BioLegend clone 93) for 15 minutes and subsequently stained with a combination of fluorophore-conjugated primary antibodies against mouse CD45 (BioLegend clone 30-F11) ...
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bioRxiv - Cell Biology 2019Quote: ... F4/80+ cells were then isolated by positive selection using a rat anti-F4/80 antibody (Biolegend, 123120) and sheep anti-rat Dynabeads (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: Freshly isolated mammary cells were incubated at 4°C for 20 min with the following antibodies from Biolegend: anti-CD24-BViolet421 (clone M1/69 ...
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bioRxiv - Immunology 2020Quote: ... Cell surface antigens were stained with combinations of the following antibodies: CD93-FITC (AA4.1, Biolegend, San Diego CA), CD23-PE (B3B4 ...
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bioRxiv - Immunology 2021Quote: ... 2.5×105−1×106 cells for each condition resuspended in 100μl PBS per tube with 1μl/100 μl Zombie Aqua Fixable Live Cell Detection reagent (Biolegend). Tubes were incubated for 15 minutes in the dark at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Surface staining was performed using appropriate dilutions of antibodies in 100 μL of cell staining buffer (Biolegend, CA) for 30 min in the dark at 4°C or on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... 3× before being incubated with APC-conjugated rat anti-CD83 antibody (1.25 μL/10×106 cells; Biolegend, 121510) for 30 minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with a mix of Fc Block (anti-mouse CD16/CD32 mAb; clone 2.4G2, BioLegend) and fluorophore-conjugated anti-mouse mAbs.
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bioRxiv - Immunology 2022Quote: ... After 1hr cells in the bottom chamber (migrated) were analysed by flow cytometry with precision count beads (Biolegend).
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in FACS buffer (PBS + 4% FBS) supplemented with Fc block (clone 93; BioLegend Cat. 101320) and incubated at 4°C for 5 minutes before staining ...
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bioRxiv - Immunology 2022Quote: ... The live cells were identified by trypan blue staining and stained according to the manufacturer’s protocol (Biolegend, USA). Briefly ...
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bioRxiv - Immunology 2022Quote: Spleens were processed into single-cell suspensions and stained using the following antibodies: B220-BV605 (RA3-6B2, Biolegend), CD86-PE/Cy5 (GL-1 ...
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bioRxiv - Immunology 2022Quote: ... 5x105 cells were plated per well of a 48-well plate coated with CD3 (5 µg/ml) (Biolegend) and cultured in regular T cell media (RPMI 1640 supplemented with 10% heat inactivated FBS (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... or spleen mononuclear cells were lysed for 10-15 minutes with RBC lysis buffer (BioLegend, San Diego, CA) and washed twice with FACS buffer ...
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bioRxiv - Biochemistry 2022Quote: ... gels and western blotting using anti-FMRP (Cell signaling #LS-C82231, 1:500 or Biolegend #6B8, 1:1000), anti-GAPDH (Santa Cruz Biotechnology #sc-47724 ...
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bioRxiv - Immunology 2022Quote: ... 5 x 105 naïve CD4+ T cells were isolated from the spleen and mesenteric lymph nodes of HDAC3FF and HDAC3ΔIEC mice via the MojoSort Mouse CD4 Naïve T Cell Isolation (BioLegend). Cell purity was confirmed by flow cytometry and T cells injected IP into age- and sex-matched Rag1-/- recipients ...
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bioRxiv - Cancer Biology 2022Quote: ... Progenitor and stem cell subpopulations were identified by staining with the following additional markers: CD34 (clone MEC14.7; BioLegend), Fc-gamma-II/III-R (clone 93 ...
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bioRxiv - Physiology 2019Quote: ... Live cells were washed with complete media and were incubated with anti-HA antibody (1:500, Biolegend, #901513) for 2hrs at 4°C prior to fixation ...
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bioRxiv - Synthetic Biology 2020Quote: ... cells were stained with an IgG1 κ isotype control directly conjugated to PE/Dazzle 594 (BioLegend catalogue #400177).
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bioRxiv - Cancer Biology 2019Quote: One million cells were stained in 100µL of Zombie Aqua (Fixable Viability Kit BioLegend; San Diego, California, USA) for twenty minutes at room temperature ...
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bioRxiv - Pathology 2020Quote: ... supernatant and attached cells were collected and stained with Annexin V-FITC (640905, BioLegend, St. Diego, CA, USA) and PI (Cayman Chemical ...
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bioRxiv - Cancer Biology 2020Quote: ... with simultaneous depletion of mouse cells stained with anti-mouse-specific antibodies directed against CD45 and CD31 (Biolegend).
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bioRxiv - Cancer Biology 2020Quote: ... anti-Phospho-c-Jun (Ser73) (D47G9) (Cell Signalling, #3270) and Ly-6A/E (Sca-1) (Biolegend, cat#108101). Appropriate Alexa Fluor labelled secondary antibodies (ThermoFischer Scientific ...
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bioRxiv - Microbiology 2020Quote: ... adherent monolayers were detached by incubation at 37°C for 5 mins with Accutase Cell Detachment Solution (BioLegend). After washing ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed twice in PBS and stained with near infrared Zombie viability dye per manufacturers protocol (Biolegend). Cell surfaces were immuno-stained with the following cocktails of fluorochrome-conjugated Abs (Biolegend) ...
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bioRxiv - Cell Biology 2021Quote: ... 50 μl of blood was resuspended in 1 ml of red blood cell (RBC) lysis buffer (Biolegend™) for 10 min on ice followed by two washes with 1 ml phosphate buffer saline (PBS) ...
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bioRxiv - Immunology 2020Quote: ... Live/dead cell markers Zombie Red™ (423109) or Zombie Violet™ (423113) dyes were purchased from Biolegend.
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bioRxiv - Immunology 2019Quote: ... cells were washed twice with FACS buffer and stained with anti-CD154 BV510 and anti-CD279 BV421 (Biolegend) and Live/dead fixable near IR APC-Cy7 in FACS buffer for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... After a quick wash with DPBS cells were stained with Zombie Red™ Fixable Viability Kit (#423110, BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... For the basal cells enrichment experiment and subpopulations isolation, CD271 (ME20.4, 345106) and CD66 (ASL-32, 342306) (Biolegend) were also included ...
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bioRxiv - Genetics 2021Quote: ... before staining cells with a mixture of antibodies in PBS containing the live/dead marker Zombie Aqua (BioLegend). Antibodies against the following antigens were used [indicating antigen-fluorophore (clone)] ...
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bioRxiv - Immunology 2021Quote: ... Fc-blocked cells were initially stained with FITC-conjugated anti-CD4 and BV421-conjugated anti-CD25 (102034; BioLegend) antibodies ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated for 30 min with the following antibodies: PE-anti-Foxp3 (259D, BioLegend 3202080 Lot B293618) and AF700-anti-Ki-67 (B56 ...
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bioRxiv - Immunology 2020Quote: ... Cells were pelleted and washed with PBS before staining with TruStain FcX anti-mouse CD16/32 (Biolegend 101319) or TruStain FcX anti-human (Biolegend 422301 ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained with specific marker of populations of interest (Ly6G-BD BioSciences, Ly6C-BioLegend, CD11b-BD BioSciences) and neutrophils (Ly6Ghi ...
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bioRxiv - Immunology 2021Quote: Naïve CD4+ T cells were isolated from spleens and lymph nodes of BDC-2.5 transgenic NOD mice using the Mouse CD4 Naïve T Cell Isolation Kit (BioLegend). Naïve T cells were further selected positively by treatment with anti-CD62L biotinylated antibodies (BD Pharmingen ...
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bioRxiv - Immunology 2021Quote: ... Cells were also stained to measure activation with the following markers: CD137 APC (4:100; Biolegend Cat# 309810), OX40 PE-Cy7 (2:100 ...
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bioRxiv - Immunology 2020Quote: CH12 cells were stimulated to undergo CSR to IgA by treatment with 1-5 μg/mL αCD40 (BioLegend), 5 ng/ml TGFβ (R&D Systems ...
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bioRxiv - Immunology 2021Quote: ... cells were first stained with Zombie Aqua fixable live/dead exclusion dye per manufacturer’s instructions (BioLegend cat #423101), followed by surface staining in FACS buffer containing 2.5% FBS ...
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bioRxiv - Immunology 2020Quote: ... 2.5 × 105 CTV-labelled T-cells were stimulated with 5µg/ml of plate-bound anti-CD3 (300331, Biolegend) and 5µg/ml of soluble anti-CD28 (302913 ...