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Citations for BioLegend :
6251 - 6300 of 7138 citations for Primary Human Melanocyte Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: BMDMs were cocultured with naïve CD4+ T cells and differentiated into Tregs in the presence 0.5 μg/mL (anti-CD3 BioLegend), 1ng/mL TGFβ (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... and unspecific binding of antibodies to Fc receptors was blocked by incubating the cells with TruStain FcXTM (Biolegend) diluted 1:50 in FACS buffer for 15 min at 4 °C ...
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Fine tuning of CpG spatial distribution with DNA origami for improved therapeutic cancer vaccinationbioRxiv - Synthetic Biology 2023Quote: ... CD8 OT-I cells and CD4 OT-II were isolated by MojoSort kit purchased from BioLegend (#480035, #480033) according to the manufacturer protocol ...
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bioRxiv - Immunology 2023Quote: ... 2.5×105-1×106 cells for each condition resuspended in 100μl PBS per tube with 1μl/100 μl Zombie Aqua Fixable Live Cell Detection reagent (BioLegend). Tubes were incubated for 15min in the dark at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were labeled with an antibody cocktail containing Anti-CD14-PE/Cy7 (Clone 63D3, 367111, BioLegend, 1:100), Anti-CD80-APC (Clone 2D10 ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were washed with PBS containing 1% FBS and incubated with APC anti-His Tag Antibody (BioLegend) for 20 minutes on ice ...
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bioRxiv - Bioengineering 2023Quote: ... The following antibodies were mixed and added directly to the cell suspension: BV421 CD86 (1:200, Biolegend, 105031), PacBlue Ly6G (1:150 ...
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bioRxiv - Immunology 2023Quote: ... Cells were plated in a 96-well round-bottom plate coated with 3 μg/ml anti-CD3 (Biolegend), uncoated well or with PMA (1 ng/ml ...
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bioRxiv - Microbiology 2023Quote: ... a small portion of cells were taken to assess infection by staining with CD90-AF700 antibody (Biolegend, 140323) for 20 minutes (1:1000 dilution in FACS Buffer) ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were subjected to a live/dead staining with the Zombie NIR™ Fixable Viability Kit (423105; BioLegend) and incubated in the dark for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... Cells were stained with an allophycocyanin (APC)-anti-CD14 or APC-anti-mouse IgG1 isotype control antibody (BioLegend) on ice ...
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bioRxiv - Immunology 2023Quote: ... stained cells were further incubated with DNA-barcoded anti-biotin and sample hashtag (anti-MHC-I) antibodies (BioLegend) for 20 minutes in PBE ...
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bioRxiv - Immunology 2023Quote: ... cells were washed with FACS buffer and stained with goat-anti-mouse IgG-Alexa Fluor647 (1:400, Biolegend) in FACS buffer for 20 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Cells were labelled with live/dead and surface stains prior to fixation and permeabilization with fixation buffer (Biolegend) and intracellular staining permeabilization wash buffer (Biolegend) ...
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bioRxiv - Microbiology 2023Quote: ... An early activation marker of T cells was also monitored using PE-Conjugated CD-69 antibody (Biolegend, 310906). Analysis was performed on FlowJo software ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently washed with PBS with ice cold 2mM EDTA and stained with TruStain FcX Plus (Biolegend) and LIVE/DEAD Fixable Blue on ice for 30 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... resuspended in fluorescence-activated cell sorting (FACS) buffer (2% BSA in PBS) and stained with 7-AAD (BioLegend) as a dye for cell viability assay ...
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bioRxiv - Immunology 2023Quote: ... after which 2.5 x 105 cells were resuspended in CITE-seq buffer containing TruStain FcX blocker (Biolegend 422302) and MojoSort CD45 Nanobeads (Biolegend 480030) ...
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bioRxiv - Bioengineering 2022Quote: ... resuspended in fluorescence-activated cell sorting (FACS) buffer (2% FBS in PBS) and stained with 7-AAD (BioLegend), and the indicated cell-surface marker ...
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bioRxiv - Cancer Biology 2023Quote: ... splenocytes were processed as described above and adherent tumor cells were concurrently collected for Annexin V (Biolegend, 640920) and 7-AAD staining (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... were stimulated with 500x Cell Activation Cocktail containing 40.5 μM phorbol-12-myristate 13-acetate (PMA) and 669.3 μM ionomycin (Biolegend) in the presence of 5 μg/mL BFA (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... MA9 (passaged through Rag2-/-γc-/- mice) or non-transformed Rag2-/-γc-/- bone marrow (BM) cells and 0.01μg/mL soluble CD3 (2C11, Biolegend). Dilution of CTV on CD4+ and CD8+ T cell populations was evaluated by flow cytometry ...
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bioRxiv - Immunology 2022Quote: ... Erythrocyte-depleted BM cells were stained with antibodies including PE conjugated anti-CD19 (6D5, Biolegend, San Diego, CA), allophycocyanin conjugated anti-B220 (RA3-6B2 ...
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bioRxiv - Immunology 2023Quote: ... stained with cell surface antibodies at 1:1200 (anti-CD8: PerCP-Cyanine5.5, eBioscience, # 45-0081-82, or Biolegend, #100734 ...
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bioRxiv - Immunology 2023Quote: Step-by-step protocol was followed for flow cytometry analysis for cell suspension samples (Biolegend, San Diego, CA). For T cell activation experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... Resuspended cells were then pooled into a single tube and incubated with Tru-Stain Fc blocking solution (Biolegend) for 10 mins at RT ...
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bioRxiv - Immunology 2023Quote: ... Binding of anti-TIGIT antibodies HB0036/HB0030 to cell surface was detected by APC-anti his (Biolegend/362605). For ELISA based assay of anti-TIGIT antibodies in tumour digest ...
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bioRxiv - Cancer Biology 2023Quote: ... All cell suspensions were centrifuged at 1500x rpm for 5 minutes and resuspended in RBC lysis buffer (Biolegend) for 5 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... freshly sorted cells were stained ex vivo or after stimulation with carrier-free recombinant mouse IL-25 (Biolegend) at a concentration of 100 ng/ml in complete RPMI medium for 36 h ...
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bioRxiv - Neuroscience 2023Quote: ... Cell suspensions were first stained for viability using Zombie NIR™ Fixable Viability Kit (BioLegend, San Diego, CA) for 20 min at room temperature in PBS combined with FcR blocking antibody (1 μg ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 million cells were incubated for 20 min at room temperature with Zombie viability dye (Biolegend ref 77143). Then cells were incubated in PBS + 10% FBS to block Fc receptors ...
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bioRxiv - Molecular Biology 2023Quote: Cells were trypsinized and stained with Annexin V and 7-AAD using APC Annexin V staining kit (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... cells were fixed with 2% PFA for 20min and permeabilized with Perm/Wash buffer (Biolegend, Cat No.421002) for 30mins ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant of cell culture was collected and analyzed by LEGENDPlexTM to analyze the concentrations of cytokines (BioLegend). BMDCs were collected and stained with live/dead dye (Cat#L34957 ...
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bioRxiv - Bioengineering 2023Quote: The T cell phenotypic characterization panel during tumor challenge assays consisted of: Anti-CD4 BV785 (BioLegend, Cat. # 317442), Anti-CD8 Pacific Blue (BioLegend ...
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bioRxiv - Bioengineering 2023Quote: The weekly tumor challenge panel for T cell tracking consisted of: Anti-CD4 APC-Cy7 (BioLegend, Cat. # 317418), Anti-CD8 Pacific Blue (BioLegend ...
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bioRxiv - Bioengineering 2023Quote: The T cell activation and exhaustion marker panel during tumor challenge assays: Anti-CD4 BV785 (BioLegend, Cat. # 317442), Anti-CD8 Pacific Blue (BioLegend ...
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bioRxiv - Neuroscience 2024Quote: ... centrifuged at 1500rpm for 5mins at 4°C and resuspended in 500µl Cell Staining buffer (BioLegend, Cat#: 420201) containing 1:4000 dilution of SYTOX Blue (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... centrifuged at 1500rpm for 5mins at 4°C and resuspended in 500µl Cell Staining buffer (BioLegend, Cat#: 420201) containing 1:4000 dilution of SYTOX Blue (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... centrifuged at 1500rpm for 5mins at 4°C and resuspended in 500µl Cell Staining buffer (BioLegend, Cat#: 420201) containing 1:4000 dilution of SYTOX Blue (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumor cells were washed with 1xPBS and blocked with 5% mouse Fc blocking reagent (Biolegend, San Diego, CA) in FACS buffer (1% FBS and 1 mM EDTA in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 X10^5 cells were then resuspended to be stained with 5ul of Annexin V-FITC (#640945, Biolegend) in 100ul of Annexin V binding buffer (10mM HEPES ...
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bioRxiv - Microbiology 2023Quote: ... 107 cells per sample were then incubated with 10 μl of Fc-blocker (anti-CD16/CD32 antibody, Biolegend) for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... Fc-receptors were first neutralized by incubating cells on ice for 5-10 minutes with TruStain FcX (BioLegend) at 2 μg/mL in 100 μL of blocking buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Cytokine release was measured in cell culture supernatants using ELISA per the manufacturer’s protocol with slight modifications (Biolegend). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... The single-cell suspensions were incubated in mouse Fc block (anti-CD16/32) antibody (Biolegend, 101335, 1:300) and then stained with the appropriate antibodies in FACS Buffer as described previously [33] ...
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bioRxiv - Genetics 2023Quote: ... Cells were pooled following staining with unique hashtag antibody oligonucleotide-conjugates (HTO) (30 minutes) staining (Biolegend, TotalSeq-B). Pooled samples were immediately run on a 10x Chromium Controller after preparation with a 10X Chromium 3’ library preparation kit (10X Genomics ...
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bioRxiv - Cancer Biology 2023Quote: ... A far-red BST2 antibody was used to label BST2-expressing cells (diluted 1:40, BioLegend, cat. 348416). Cell viability was determined by Sytox Blue (diluted 1:1000 ...
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bioRxiv - Immunology 2023Quote: TNFα and CCL5/RANTES were quantified in cell culture supernatants using mouse ELISA kits (BioLegend, R&D Systems) according to manufacturers’ instructions ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed with PBS prior to staining with Zombie NIR Fixable live/dead dye (1:500) (Biolegend) for 20 min at 4°C ...