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Citations for BioLegend :
6501 - 6550 of 7138 citations for Primary Human Melanocyte Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... CD73- CD4+ CD25- Foxp3- newly generated CD4SP thymocytes were sorted from Foxp3eGFP-Cre-ERT2 reporter mice and reconstituted at a concentration of 20,000 cells/100uL in complete RPMI + 100ng/mL recombinant mouse IL-7 (Biolegend). CD4SP thymocytes and DC subsets were co-cultured at a 2:1 ratio in round-bottom 96-well plates for 4 days and then analyzed by flow cytometry on day 5 to evaluate the percentage of Tregs generated in vitro.
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bioRxiv - Immunology 2023Quote: Single-cell suspensions from the tumors and draining lymph nodes were first stained with 1 μL Zombie Aqua (BioLegend) to exclude dead cells ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were resuspended in 1mL of PBS and live/dead dye used at 1:500 (Zombie NIR, BioLegend #423106). Cells were washed with 5mL of FACS buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were stained with the following monoclonal antibodies to determine cellular architecture of most abundant spleen and immune cells (Table S10): anti-mouse CD45.2 (Biolegend), anti-mouse Gr-1 (eBioscience) ...
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bioRxiv - Genomics 2023Quote: ... Cells were stained on ice in the dark for 30 mins with CD34-APC (clone 581; Biolegend; 1:100), CD38-PE/Cy7 (clone HB7 ...
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bioRxiv - Immunology 2023Quote: ... and then single-cell suspensions were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Biolegend, #426803) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were labelled with rat-anti-Prominin-1 antibody (Brilliant Violet 421-conjugated; BioLegend, #141213, Lot# B255870, 1:1000) and Alexa Fluor 647-conjugated recombinant human EGF (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... resuspended in 50ul/staining volume of Fc block mastermix (5ul Fc block and 45ul cell staining buffer, both Biolegend; one staining volume = up to 2 x 106 cells ...
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bioRxiv - Neuroscience 2024Quote: Cytokine levels in supernatant collected from BALF or cell culture were determined with commercially available ELISA kits from Biolegend, R&D systems ...
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bioRxiv - Biochemistry 2024Quote: ... We determined the amounts of IL-2 in the culture supernatant of activated or naïve CD4+ T cells with an ELISA kit (BioLegend). We measured the amounts of IgE in the serum with a commercially available ELISA kit (Sigma-Aldrich).
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bioRxiv - Biochemistry 2024Quote: ... Cells were stained in 100 µL 1X PBS containing 0.5% (v/v) Zombie UV fixable viability dye (BioLegend # 423108) on ice for 20 min ...
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bioRxiv - Immunology 2024Quote: ... HVEM binding to BTLA was assessed by incubating cells with the HVEM-Fc recombinant protein (20 µg/ml; Biolegend) followed by a staining with an Alexa488 conjugated-anti-IgG1 secondary Ab (clone HP6069 ...
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bioRxiv - Microbiology 2024Quote: ... This protocol consistently resulted in >80% of cell viability as measured by tryphan blue or calcein violet-AM (BioLegend).
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bioRxiv - Immunology 2024Quote: Bulk cultured T cells were stimulated with 5 µM peptides for 5hr in the presence of brefeldin A (Biolegend). The cells were collected and stained with specific antibodies against surface markers ...
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bioRxiv - Immunology 2024Quote: ... and the endothelial cells were separated using Dynabeads coupled to anti-CD31 antibody and anti-ICAM2 antibody (Biolegend, USA) according to the manufacturer’s instructions (Thermo fisher scientific ...
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bioRxiv - Immunology 2024Quote: The following antibodies were used for staining mouse tissue sections for imaging or isolated cells for flow cytometry: B220 PCPCy5.5 (clone RA3-6B2; Biolegend), B220 PE/Fire 700 (clone RA3-6B2 ...
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bioRxiv - Immunology 2024Quote: Single cell suspensions were first washed in PBS and then incubated with 50 μl Zombie UV viability dye (BioLegend) for 10 min at room temperature in the dark ...
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bioRxiv - Neuroscience 2024Quote: ... cells were placed on ice for 15 mins and treated with cold Mouse monoclonal antibody against LAMP1(#328611; Biolegend) specific to the N terminal luminal epitope of the antigen ...
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bioRxiv - Immunology 2024Quote: Liver cells were collected by centrifugation and then incubated with Fc blocking antibody (purified anti-mouse CD16/32, BioLegend) on ice for 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were then resuspended in 50uL of PBS containing fluorophore-conjugated antibodies for CD11b (1:100, Biolegend, 101216), MHCII (1:100 ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T cells were identified with CD3-Pacific Blue (cat# 300431) or CD8-Pacific Blue (cat# 301033) antibodies (Biolegend). Activation of CD8+ CAR-T cells was determined with CD25 staining using CD25-APC antibody (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed and then fixed and permeabilized using the True-Nuclear™ Transcription Factor Buffer Set (BioLegend 424401). Intracellular staining antibodies (IFNγ ...
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bioRxiv - Immunology 2024Quote: Skin cytokines or growth factors were quantified using LegendPlex Mouse Cytokine release syndrome and Hematopoietic stem cell kits (Biolegend). Briefly ...
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bioRxiv - Immunology 2024Quote: ... and single cell preparations were obtained by passing the cell mixture through a PluriStrainer (500 – 70µM cell strainers; 43-50500, PluriSelect) before blocking with Fc block for 10 min (422302, BioLegend). CD45+ cells were enriched using anti-CD45 magnetic MicroBeads and LS columns (130-045-801 and 130-042-401 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were washed with FACS buffer (PBS, 1% BSA, 0.1% NaN3) and incubated with Zombie Green viability dye (BioLegend) to exclude dead cells ...
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bioRxiv - Immunology 2024Quote: ... to lyse the red blood cells and then stained on ice for 30 minutes with the following antibodies: BV421-labeled CD45.1 (A20, BioLegend), BV785-labeled CD45.2 (104 ...
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bioRxiv - Immunology 2024Quote: ... cells were resuspended in 50 µL Fc blocking medium (2.5 µg/ml purified anti-mouse CD16/32 Antibody (BioLegend) in Cell Staining Buffer (BioLegend)) ...
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bioRxiv - Synthetic Biology 2021Quote: ... cells were stained for surface markers and then prepared for intracellular staining using True-Nuclear Transcription Factor staining kits (Biolegend). For experiments demonstrating stimulation response ...
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bioRxiv - Synthetic Biology 2021Quote: ... The knock-out of the T cell receptor (TCR) was assessed with the absence of signal after staining 1:200 with APC-labeled anti-CD3ε (Biolegend). Each CAR construct contained a Strep tag which allowed for a two-step staining to validate successful knock-in ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then stained in FC buffer (2% FCS in PBS) with anti-CD3-APC conjugated antibodies (1:80; BioLegend) and LIVE/DEAD Fixable Near-IR Dead Cell Stain (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... After staining BM was washed two times with cell stain buffer followed by a second incubation step with PE-conjugated Streptavidin (405204, Biolegend) for 20 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... before plating the cells in petri dishes in RPMI 1640 medium containing 10 % heat-inactivated FCS and 50 ng/ml M-CSF (BioLegend) or 50 ng/ml GM-CSF (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained directly for determining NK cell numbers and Ki67 staining or incubated for 4 hours in medium containing Brefeldin A (Biolegend) and Monensin (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... the cell suspensions were stained with the following antibodies: PE anti-mouse Ly-6C (BioLegend, 128008, clone HK1.4, 1:200), PerCP/Cyanine5.5 anti-mouse/human CD11b (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained with the following antibodies and stains (in multiple sets to prevent fluorophore overlap): CD4-FITC (BioLegend), CD8-PECy5 (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... total thymocytes were processed to single cell suspension in 10% FBS/HBSS (20×106 cells/ml) and incubated with rat anti-mouse CD24 antibodies (M1/69, Biolegend) (30 μg/100 ×106 cells ...
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bioRxiv - Immunology 2021Quote: ... Antibodies for flow cytometry and cell isolation against the following proteins (all mouse unless otherwise indicated) were obtained from BioLegend, eBioscience ...
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bioRxiv - Immunology 2021Quote: Cytokine levels in supernatants from stimulated cells were determined using the LEGENDplex™ Mouse anti-viral response panel (Biolegend, USA) as per manufacturer’s instructions and read on BD Accuri™ C6 Plus Flow Cytometer (BD Biosciences) ...
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bioRxiv - Microbiology 2021Quote: ... the cells were incubated in PBS-5% FCS on ice for 30 min with rabbit anti-K10 (1:1000) (Biolegend), and K10 was visualized with anti-rabbit AF488 (Life Technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... and transferred to a 2 mL Eppendorf tube for immunostaining for 30 minutes on ice for the immune cells (CD45-PE/Cy7, 1:250, 103114, BioLegend), endothelial cells (ICAM2-A647 ...
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bioRxiv - Immunology 2021Quote: ... cells were washed in staining buffer (1% BGS-supplemented PBS) and incubated with anti-Flag-PE (clone L5; BioLegend #637310) and Ghost Dye Violet 510 (Tonbo Biosciences #13-0870-T100 ...
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bioRxiv - Immunology 2020Quote: ... Intracellular staining for Foxp3 was performed on single cell suspensions from spleen and LNs specifically using a FOXP3 Fix/Perm Kit (BioLegend).
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bioRxiv - Immunology 2022Quote: We quantified CD4+ and CD8+ T cells by incubating 100 μl of whole blood with anti-CD8-Brilliant Violet 421 (clone SK1; BioLegend), anti-CD4-APC (clone L200 ...
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bioRxiv - Cell Biology 2022Quote: ... 105 - 106 cells were resuspended in FACS buffer with 1 µg/ml TruStain FcXTM anti-mouse CD16/32 antibody (BioLegend) to block for 5 minutes before adding conjugated surface antibodies in FACS buffer at a 2X concentration ...
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bioRxiv - Genomics 2020Quote: ... The remaining 450μl of cell suspension was stained with 15 μl of each of the following antibodies: CD3 APC (Biolegend, 300411), CD4+ FITC (BD ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... activated T-cells were treated with HDACI as described before and after 72 h total protein acetylation of the cells was determined by flow cytometry using PE-coupled anti-Acetylated Lysine Antibody (Biolegend). Cells were washed with PBS ...
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bioRxiv - Immunology 2021Quote: ... The resultant cell suspension was stained with the following antibodies directed against cell surface antigens for 30 minutes at 4°C: CD45::BV421 (H130, BioLegend) and HLA-DR::PE-Cy7 (L243 ...
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BET protein inhibition regulates macrophage chromatin accessibility and microbiota-dependent colitisbioRxiv - Immunology 2021Quote: ... LPMCs and splenocytes were washed with cold 1x PBS and stained for viability followed by cell-surface marker staining for CD45 (1:100; Clone 30-F11, BioLegend), CD3ε (1:300 ...
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bioRxiv - Immunology 2021Quote: ... cell-free supernatants were collected followed by ELISA to detect IL-10 and TGFβ using capture and detecting antibodies (Biolegend). Other M2 phenotype characteristics including the cell surface expressions of CD206 ...
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bioRxiv - Immunology 2021Quote: FACS purified cell subsets were labeled by incubation for 30 minutes at 4°C with TotalSeq anti-mouse Hashtag antibodies (BioLegend), then washed ...