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Citations for BioLegend :
6001 - 6050 of 7138 citations for Primary Human Melanocyte Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 1.106 cells were blocked in PBS/1mM EDTA/1% FBS/1µg of TrueStain FcX™ (Biolegend, 101320).
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bioRxiv - Microbiology 2022Quote: ... 5e5 to 1e6 cells were resuspended in 22.5μl of Staining Buffer and incubated with 2.5μl of TruStain FcX (BioLegend #422302) for 10 minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... Overnight fixed cells were permeabilized with 1X Permeabilization buffer (True Nuclear 10X Perm, Catalogue No. 73162, Biolegend) for intracellular staining ...
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bioRxiv - Immunology 2022Quote: Cells enriched from various tissues were stained for viability using the Zombie Fixable Viability Kit (Biolegend®) then incubated with anti-CD16/32 Fc block (BioXcell) ...
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bioRxiv - Microbiology 2022Quote: ... 1×106 cells were plated and then incubated with Fc block (Biolegend; cat#101320; 1:100 dilution) and surface stained for 20 min at 4°C in the dark ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated 30 minutes with 1μM gp33 before addition of 5μg/ml of brefeldin A (Biolegend). The cells were then incubated 4 hours 30 minutes before staining.
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bioRxiv - Immunology 2022Quote: ... Identification of MAIT T cells was determined by staining with Vα7.2-PE and CD161-BV421 antibodies (Biolegend). CD4- population for calculating MAIT cell percentage in PBMC was determined by gating out CD4+ cells stained with CD4-BV605 antibody (Biolegend) ...
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bioRxiv - Immunology 2023Quote: ... The cells were then stained with FITC Annexin V Apoptosis Detection Kit with 7-AAD (BioLegend 640922) following the manufacturer’s instructions and subjected to flow cytometry ...
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bioRxiv - Immunology 2024Quote: ... Fixation/permeabilization of cells was conducted for intracellular staining using the eBioscience Foxp3 fixation/permeabilization kit (BioLegend) or Tonbo Foxp3 / Transcription Factor Staining Buffer Kit.
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bioRxiv - Molecular Biology 2022Quote: ... as well as antibodies to cell surface markers (anti-45 AF700 [Biolegend, 147716], anti-CD3e FITC [Biolegend, 100306] ...
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bioRxiv - Immunology 2022Quote: ... T cell degranulation was also measured simultaneously with the addition of anti-CD107a PE (Biolegend, 1:200), and monensin (BD GolgiStop ...
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bioRxiv - Immunology 2022Quote: Single cell suspensions were stained for viability using the Zombie Aqua fixable viability kit (1:1000 BioLegend) and fixed with 4% paraformaldehyde (PFA ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then incubated for 1 hour on ice with AF700 conjugated CD45.2 monoclonal antibody (104, Biolegend). For scRNA-seq libraries ...
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bioRxiv - Cell Biology 2022Quote: ... and then stained with live/dead cell discriminating BioLegend Zombie Dyes (UV, NIR, Violet, or Yellow, BioLegend #423108 ...
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bioRxiv - Immunology 2022Quote: ... 0,5*106 CD4+ T cells were incubated with biotinylated 1μg/mL anti-CD3ε (Clone 145-2C11, BioLegend) alone or in combination with 2ug/mL biotinylated anti-CD28 (Clone 37,15 ...
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bioRxiv - Immunology 2024Quote: ... Cells were fixed for 5 to 10 min at room temperature in 4% paraformaldehyde fixation buffer (BioLegend). Cells were permeabilized for 30 min at 4°C in PhosFlow Perm Buffer III (BD Biosciences) ...
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bioRxiv - Cancer Biology 2024Quote: Oligonucleotides were conjugated to two antibodies against HGSOC cell surface proteins β2-Microglobulin (β2M) and CD298 (BioLegend) by iEDDA-click chemistry according to CITE-seq antibody-oligo conjugation protocol (https://cite-seq.com) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then harvested and incubated with a panel of antibodies containing anti-mouse CD19 (Biolegend,115504), anti-mouse CD3d (Biolegend ...
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bioRxiv - Molecular Biology 2023Quote: ... 57,58 Bone marrow cells were cultured with 20 ng/ml of macrophage colony-stimulating factor (BioLegend #576404) for 7 days as previously described 58 to generate BMDMs ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with inhibitors for 6 hours and then stained with Zombie Aqua (BioLegend, Cat. # 423101) following the manufacturer’s instructions.
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bioRxiv - Pathology 2024Quote: ... and the cell pellet was stained with anti-Cd31 (Alexa Fluor 488-conjugated 1:100, Biolegend, 102514), anti- Cd45 (Alexa Fluor 488-conjugated 1:100 ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated in the dark on ice for 20 minutes with APC anti-CD235a (Biolegend, #349114), APC anti-EPCAM (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... peripheral blood mononuclear cells (PBMCs) were stained with the following antibodies: allophycocyanin (APC)-anti-CD34 (581; BioLegend), FITC-anti-CD14 (61D3 ...
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bioRxiv - Immunology 2024Quote: ... cells were fixed and permeabilized using the True-Nuclear Transcription Factor Buffer Set (BioLegend Cat. No. 424401) using the manufacturer’s protocol with overnight fixation/permeabilization.
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bioRxiv - Immunology 2024Quote: ... Cells were washed once and resuspended in 100μl blocking buffer (2%FBS and 1% FcX (BioLegend, 422302) in PBS ...
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bioRxiv - Immunology 2024Quote: ... Isolated cells were treated for 5 min with TruStain FcXTM (anti-mouse CD16/CD32) (cat. #101320, BioLegend) prior to staining ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated with antibodies against TNF-α (clone: Mab11) and IL-1β (clone: CRM56) from Biolegend for 60 minutes ...
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bioRxiv - Immunology 2024Quote: ... Cells that were used in CITE-seq experiments were blocked with TruStain Fc block (BioLegend; Cat. 422301) for 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... T cell activation was assessed by fluorophore-conjugated monoclonal antibodies detecting CD137 (also known as TNFRSF9; BioLegend) and CD25 (also known as IL2RA ...
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bioRxiv - Immunology 2024Quote: ... and incubated for 2 hours after which cells were treated with 1x Brefeldin A (BioLegend; cat#: 420601) and incubated for a final 4 hours before analysis by flow cytometry for T cell activation.
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bioRxiv - Immunology 2024Quote: ... When appropriate NK cells were fixed and permeabilised using the True-Nuclear Transcription Factor Buffer set (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... were stained after cell fixation and permeabilization with True-NuclearTM transcription factor buffer set per protocol (BioLegend). Data were collected on BD LSR Fortessa or Beckman CytoFLEX flow cytometer and analyzed by FlowJo (Tree Star Inc. ...
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bioRxiv - Synthetic Biology 2023Quote: ... stable pools of cells were analyzed by flow cytometry using APC anti-Flag-tag antibody (Biolegend; 637307). Stable pools went through another round of selection in complete media supplemented with with 0.7ug/mL puromycin and 1ng/mL human prolactin or purified mouse prolactin and were analyzed by flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... The following antibodies were used for labeling cells: PE rat anti-mouse CD45 (Clone 30-F11; BioLegend), FITC rat anti-mouse CD3 (Clone 17A2 ...
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bioRxiv - Cell Biology 2023Quote: ... 1–2 million cells/100 μl were incubated with the following antibodies: anti-CD11c PeCy7 (117318, Biolegend), anti-CD11b (101212 ...
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bioRxiv - Immunology 2023Quote: ... cells were stimulated with PPD (20 μg/ml) in the presence of anti-CD28 antibody (37.51, BioLegend) for 1.5 hours and 10 μg/ml Brefeldin A (Sigma ...
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bioRxiv - Immunology 2023Quote: ... cells were stimulated for the following 5h using a combination of protein transport inhibitor (Monensin) (Biolegend, USA), Ionomycin (1 ug/ml) ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was aspirated and cells were resuspended in 25 µL FcR block per well (200x, Biolegend #101320) diluted in FACS buffer (2 % FBS ...
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bioRxiv - Synthetic Biology 2023Quote: Cytokine concentrations in cell culture supernatants were measured by ELISA using kits to detect IFNγ (Biolegend; 430104), IL-2 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Nonspecific binding was blocked by incubating cells anti-mouse CD16/32 Fc block (Biolegend; cat. no. 101310). Sytox-Green viability dye (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: For stimulation and cell culture: anti-CD3ε (145-2C11) and anti-CD28 (37.51) antibodies were from Biolegend. For cell sorting and flow cytometry analysis ...
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bioRxiv - Pathology 2023Quote: ... pure populations of known cells were be obtained by FACS isolation of CD45+ (leukocyte, Biolegend Cat#304006), CD45+/CD68+ (histiocytes ...
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bioRxiv - Cancer Biology 2022Quote: ... and cells were incubated with a blocking solution containing mouse TruStain FcX (Biolegend, San Diego, CA; #101319) before antibody staining ...
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Podoplanin expression in fibroblasts determines lymph node architecture and adaptive immune functionbioRxiv - Immunology 2022Quote: ... Cells were permeabilised for 20 minutes at 4°C using the FOXP3 Fix/perm buffer set (Biolegend) containing purified rat IgG2b anti-mouse CD16/CD32 receptor antibody ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in washing buffer (PBS 0.04% BSA) and incubated with mouse Fc blockers (FcX, Biolegend). Each sample was then incubated with either TotalSeq anti-mouse Hashtag Antibody 1 (Biolegend #155861 ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed and permeabilized with a cytokine staining buffer set (Biolegend, Cat#420801 and Cat#421002) for cytokine/chemokine staining and a Foxp3/Transcription Factor Staining Buffer Set (eBioscience/Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Cell suspensions were analyzed by flow cytometry after staining with anti-TCRβ APC (BioLegend, clone H57-597), anti-CD11b PerCpCy5.5 (BD Biosciences ...
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bioRxiv - Bioengineering 2023Quote: ... Then cells were labeled with biotinylated HEL generated in-house and anti-mouse CD19-CF594 (ID3, Biolegend), followed by staining with streptavidin-allophycocyanin and live/dead Aqua (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: ... placental single-cell suspensions were stained with PerCP/Cy5.5-conjugated anti-mouse CD34 (BioLegend, San Diego, CA), PE-conjugated anti-mouse Ly-6A/E (SCA1 ...
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bioRxiv - Immunology 2023Quote: ... Trypsinized tumor cells were counted and stained with carboxyfluorescein diacetate succinimidyl ester (CFSE, 1 μL/mL; BioLegend) following the manufacturer’s protocol and cultured overnight ...