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Citations for BioLegend :
5951 - 6000 of 7138 citations for Primary Human Melanocyte Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... mixed LN cells were activated using plate-bound anti-CD3ε (1.5 μg/mL) (2C11, BioLegend, London, UK) and soluble anti-CD28 (37.51 ...
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bioRxiv - Immunology 2020Quote: ... Cells were then fixed and permeabilized with either Perm Fix and Wash buffer (Biolegend, for cytokine staining) or the Foxp3 transcription factor staining buffer set (eBioscience ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were pelleted at 220-300 x g and resuspended in 5 mL RBC lysis buffer (BioLegend) at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Following a wash, cells were incubated (RT, 10 min) with Rhesus Fc Receptor Binding Polyclonal Antibody (Biolegend). Desired staining antibodies were added directly to this mixture at indicated dilutions (Supplemental Table 27 ...
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bioRxiv - Immunology 2022Quote: ... The abundance of Nectin-2+ monocytes and NK cell intracellular cytokine production of IFN-γ (4S.B3, Biolegend) were analyzed by flow cytometry (BD LSRFortessa™ Cell Analyzer ...
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bioRxiv - Genomics 2022Quote: ... and PE anti-DYKDDDDK Tag antibody (0.6 ul antibody per million cells in 100 ul) (Biolegend #637310) for DYKDDDDK-tagged Cas9 was carried out at room temperature for 45 minutes ...
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bioRxiv - Genomics 2022Quote: ... 150-300 Million cells were collected and stained with Zombie Violet™ fixable viability kit (Biolegend #423113) with 1:200 dilution in phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with PBS and stained with anti-CD3-Pacific Blue (1:200, Biolegend catalog #300417) and anti-CD8-AF700 (1:200 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated with an anti-INF-γ-FITC antibody (clone XMG1.2 BioLegend, San Diego, CA) at 4°C for 30 min in the dark ...
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bioRxiv - Immunology 2022Quote: ... the cell samples were stained with the following indicated antibodies in FACS buffer: anti-CD62L (161204, BioLegend), anti-CD44 (25-0441-82 ...
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bioRxiv - Immunology 2022Quote: ... Viable cells were sorted on the BD FACSMelody into polypropylene tubes based on CD8-APC (Biolegend, 100721), CD103-PE (Biolegend ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the cells were carefully washed with PBS and subsequently fixed with fixation buffer (BioLegend, San Diego, USA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2019Quote: ... The following antibodies were used to perform NK cell and monocyte sorting: CD3-Allophycocyanin (clone OKT3; BioLegend), CD14-Brilliant Violet 421 (clone HCD14 ...
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bioRxiv - Developmental Biology 2020Quote: ... Dead cells were stained with eFluor 450-fixable viability dye (eBioscience) before fixation with fixative buffer (BioLegend).
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CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... Following antibodies were purchased and used for aortic cell staining: CD45 (30F-11, BD Bioscience and Biolegend), CD31 (MEC13.3 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed twice with CSM before pooling and staining with E-Cadherin-AF647 (clone 67A4, Biolegend) and EpCAM-FITC (clone 9C4 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Plates were washed three times with PBS and blocked with Cell Staining Buffer (BioLegend, San Diego, CA). Plates were then incubated with rabbit-anti-LC3B (Cell Signaling Technologies ...
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bioRxiv - Immunology 2019Quote: ... The negatively enriched cells were stained with anti-mouse PD-1 and anti-mouse KLRG1 antibodies (BioLegend), as well as ESAT-6 tetramer (as above ...
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bioRxiv - Synthetic Biology 2020Quote: ... We evaluated the percentage of dead cells using the Zombie Violet Fixable Viability Kit (Biolegend, CA, USA). Briefly ...
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bioRxiv - Neuroscience 2019Quote: ... and cells were stained with rat anti-mouse ICAM-1 (1:100; Biolegend YN1/1.7.4 in PerCP), rat anti-mouse CD45 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... lung cells were surface stained with a biotinylated Y-Ae antibody followed by APC-Streptavidin (405207, Biolegend). Mouse H2-O expression was detected by staining intracellularly with an anti-mouse H2-Oβ antibody97 provided by L ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with monoclonal antibodies CD16-Alexa Fluor 647 clone 38G (BioLegend INC, San Diego, CA), CD56-PE-Cy5 clone CMSSB ...
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bioRxiv - Immunology 2020Quote: ... mitochondrial mass was determined in individual cells by labelling them with the probe MitoSpy Green FM (Biolegend). Green fluorescence was analyzed by flow cytometry.
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bioRxiv - Immunology 2020Quote: The isolated cells were then stained for 30 min at 4°C with the following antibodies (Biolegend): APC-Fire 750-anti CD11b (Cat ...
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bioRxiv - Genomics 2019Quote: ... freshly isolated CD4+ T cells were stained with monoclonal antibodies against CD3-PE and CD4-FITC (Biolegend) using standard procedures ...
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bioRxiv - Immunology 2019Quote: ... Cells were washed and then blocked with anti-CD16/32 followed by staining with Zombie Aqua (BioLegend) and antibodies ...
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bioRxiv - Genomics 2021Quote: ... Resultant cell pellets were resuspended in 50ul of antibody cocktail (all antibodies from Biolegend, Supplementary File 12) for 30 minutes before further washing and fixation in 2% paraformaldehyde ...
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bioRxiv - Immunology 2021Quote: Single cell suspensions were stained with Live/Dead stain (LIVE/DEAD Fixable Near-IR or Yellow, Biolegend) and Fc-Receptor block was performed (using clone 93 ...
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bioRxiv - Cell Biology 2020Quote: ... Freshly isolated cells were pre-incubated with anti-CD56 Alexa Fluor 647 (clone HCD56, Biolegend, 1:100) for 20 minutes in a conical tube then incubated on #1.5 coverslips that had been pre-coated with poly-L-lysine for an additional 20 minutes at 37°C 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed with PBS5 and stained with APC-conjugated goat anti-rat IgG (Biolegend, 1:200) for 20 min ...
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bioRxiv - Cell Biology 2019Quote: HEK293 cells stably expressing EPO-eGFP and A1At-mCherry were incubated with 1ug/mL Brefeldin A (Biolegend) for 12 hr ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were blocked with PBS containing 3% BSA and stained with Alexa Fluor 647-anti-DYKDDDDK (BioLegend) at 0.5 μg/mL or with Alexa Fluor 488-streptavidin (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... or from c-Relfl/fl and c-Relf/fNcr1Cre using MojoSort Magnetic Cell Separation (purchased from BioLegend). For anti-tumor cytotoxicity ...
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bioRxiv - Immunology 2020Quote: ... All cells were then stained in 1X PBS buffer with Zombie NIR fixable viability kit (#B262784, Biolegend) according to the manufacturer instructions ...
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bioRxiv - Immunology 2020Quote: ... and anti CD38 FITC (Stem Cell, 60131FI) and the Zombie NIR™ Fixable Viability Kit (Biolegend, 77184). Avi-tagged and biotinylated BG505 SOSIP ...
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bioRxiv - Immunology 2020Quote: ... Cells were then fixed (TrueNuclear™ TF fixation and permeabilization buffer set, BioLegend, San Diego, CA, USA) at room temperature for 60 min ...
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bioRxiv - Immunology 2020Quote: ... Equal cell numbers where incubated at room temperature for 10 minutes with zombie aqua viability dye (BioLegend), followed by 10-minute incubation on ice with blocking buffer containing 10% mouse serum with 0.25 μg/ml anti CD16/CD32 (BioLegend) ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed and stained for 30 minutes in CD44 PerCP Cy5.5 (Biolegend clone IM7, 1:400), B220 BV510 (Biolegend clone RA3-6B2 ...
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bioRxiv - Microbiology 2021Quote: ... cells were stimulated with PPD (20 μg/ml) in the presence of anti-CD28 antibody (37.51, BioLegend) for 1.5 hours and 10 μg/ml Brefeldin A (Sigma ...
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bioRxiv - Immunology 2020Quote: ... Coated plates were washed and cells were plated with 1 µg/ml soluble αCD28 antibody (37.51, Biolegend). Bone marrow-derived dendritic cells (BMDC ...
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bioRxiv - Immunology 2021Quote: ... but it was followed by a step of red blood cells lysis with RBC lysis buffer (Biolegend). Data were acquired in BD FACS Canto II (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used for myeloid cell phenotyping: BV421 or PE-Cy5-conjugated anti-CD11b (BioLegend), APC-Cy7-conjugated anti-Ly6G ...
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bioRxiv - Immunology 2021Quote: ... and 250,000 cells were resuspended in 100 µl of FACS buffer containing TruStain FcX blocker (Biolegend 422302) and MojoSort CD45 Nanobeads (Biolegend 480030) ...
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bioRxiv - Cancer Biology 2021Quote: ... We excluded dead cells in samples originating from transplanted 3T3 tumors using Zombie Aqua labeling (423102, Biolegend) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... and cells were then washed and incubated with TruStain fcX (CD16/CD32. Clone 93, BioLegend, catalog # 101320), and stained with surface antibodies for indicated immune cells ...
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bioRxiv - Immunology 2022Quote: ... B cells were stimulated for 2 days with 10 ug/ml of anti-CD40 (Biolegend, Cat#102812) and 10 ng/ml of rec ...
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bioRxiv - Immunology 2022Quote: ... the stained cells were distributed across the three 96-well plates provided with the LEGENDScreen kit (Biolegend), each well containing a unique PE-labeled exploratory antibody as well as isotype controls and blanks ...
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bioRxiv - Immunology 2022Quote: ... FSC-A/H and dead cell exclusion by using a Fixable Viability Kit (Near-IR staining, Biolegend).
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bioRxiv - Immunology 2022Quote: ... B cells were stained with anti CD21-FITC and anti CD23-PE antibodies (Biolegend, San Diego, CA) and sorted at the BD FACS-Aria Fusion ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated with a Live/Dead amine reactive viability dye (Zombie ultraviolet (UV) dye) (BioLegend) diluted 1:2000 in 10 μl 1X PBS for 15 min at RT in the dark to facilitate dead cell exclusion ...