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Citations for Roche :
2651 - 2700 of 9048 citations for Cow WAP kazal immunoglobulin kunitz and NTR domain containing protein 2 WFIKKN2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The medium from each well was removed and replaced with fresh medium containing 10 µl of 12 mM MTT as per the manufacturer’s instruction (Roche). After 4 h of incubation ...
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bioRxiv - Immunology 2023Quote: ... Cells were lysed by sonication in 700 mM NaCl;20 mM HEPES 7.5 containing 1 protease inhibitor tablet (Roche) + 5ul Turbonuclease ...
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bioRxiv - Genomics 2023Quote: ... and the pellet resuspended in 100 µl of ice-cold RIPA lysis buffer containing 1× protease inhibitor cocktail (Roche), incubated for 30 min on ice ...
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bioRxiv - Molecular Biology 2023Quote: ... Precipitants were washed with acetone and dissolved in SDS-PAGE sample buffer containing 4 mM Pefabloc SC (11429876001, Roche). Equivalent protein amounts were subjected to immunoblotting analysis.
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bioRxiv - Cell Biology 2023Quote: ... The remaining cortical tissue was minced and transferred to 10 mL HBSS containing Collagenase A (Roche, 50-100-3278) at 200 units/ml ...
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bioRxiv - Microbiology 2023Quote: ... using 100 nL containing (final concentration) 1 × Light Cycler 480 SYBR® Green I Master Mix (Roche Inc., USA), nuclease free PCR-grade water ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting cell pellet was resuspended in a lysis buffer of Buffer A + 0.5% Triton X-100 containing 5% protease inhibitor cocktail cOmplete (Roche, 118614500 ...
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bioRxiv - Cancer Biology 2023Quote: ... Each suspension was then supplemented with 0.1 mL of RPMI containing 1.25 mg mL−1 Liberase TM (Roche, 5401119001) and 20 mg mL−1 DNase I (Roche ...
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bioRxiv - Genomics 2023Quote: ... Cells were washed twice in cold PBS and scraped off the plates in cold PBS containing protease inhibitors (Roche, Complete 11836170001 and Halt ...
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bioRxiv - Genomics 2023Quote: Fresh pinch biopsies were washed twice in DPBS before being coarsely chopped with a scalpel and transferred into 0.5 mL DPBS solution containing 0.1 WU ml−1 Liberase TH (Roche, 5401151001) and 0.5 U ml−1 RQ1 DNase I (Promega ...
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bioRxiv - Systems Biology 2023Quote: ... The left lobes of the lung were then inflated with digestion solution containing 1.5 mg/mL of Collagenase A (Roche) and 0.4 mg/mL DNaseI (Roche ...
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bioRxiv - Microbiology 2023Quote: ... PCR was performed with one microliter of DNA from the BAL in a final volume of 20 µl containing 0.2 µl polymerase (KAPA LongRange HotStart, Roche), the provided buffer ...
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bioRxiv - Plant Biology 2023Quote: ... the cells were harvested in 20 mM potassium phosphate buffer (pH 8.0) containing 500 mM NaCl and cOmplete EDTA-free protease inhibitor cocktail (Roche). The inclusion bodies were pelleted from sonicated cells at 3000 × g for 15 minutes and solubilized in 20 mM potassium phosphate buffer (pH 8.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell pellets were lysed with 200 μl buffer containing 8 M urea and 200 mM EPPS (pH at 8.5) with protease inhibitor (Roche) and phosphatase inhibitor cocktails 2 and 3 (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... then the pellet was resuspended in 40 ml binding buffer (50 mM NaPi, 300 mM NaCl, 5 mM imidazole, pH 7.5) containing protease inhibitor (Roche cOmplete EDTA-free protease inhibitor cocktail ...
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bioRxiv - Neuroscience 2023Quote: ... containing 0.1 mM CaCl2 and 1.0 mM MgCl2 and lysed in 8 urea with Complete protease inhibitor (Roche 11245200) and/or Halt phosphatase inhibitor (Pierce A32957 ...
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bioRxiv - Biochemistry 2024Quote: ... pellets were resuspended in buffer A (25mM Tris, pH 7.5, 300mM NaCl, 30 mM imidazole,) containing cOmplete EDTA-free protease inhibitor cocktail (Roche), 1,5µM pestatin and 2µM bestatin ...
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bioRxiv - Cell Biology 2024Quote: ... and the cell pellet was gently resuspended in 1 ml ice-cold vPBS containing EDTA-free protease inhibitor (Roche). The cells were again pelleted by centrifugation (1000 x g ...
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bioRxiv - Cell Biology 2024Quote: ... or ice-cold 8% (v/v) paraformaldehyde solution/ 0.2% (v/v) glutaraldehyde in vPBS containing EDTA-free protease inhibitor (Roche) was added to the resuspended cells ...
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bioRxiv - Cell Biology 2024Quote: ... If the cells were fixed with 4% (v/v) paraformaldehyde solution and 0.1% (v/v) glutaraldehyde in vPBS containing EDTA-free protease inhibitor (Roche), the coverslips were directly mounted on glass slides after cell attachment ...
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bioRxiv - Cell Biology 2024Quote: Cells or tissues were washed with PBS and lysed in an appropriate amount of protein lysis buffer (TritonX100 -1%, Tris 7.4 -50mM, EDTA -5mM, NaCl -150mM, and glycerol -5%) containing Protease Inhibitor Cocktail (Roche). We used Phosphatase Inhibitor Cocktail (Millipore ...
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bioRxiv - Neuroscience 2024Quote: ... Human tissues were homogenized in TBS (20 mM Tris-HCl, 140 mM NaCl, pH 7.5) containing protease and phosphatase inhibitors (Roche). Following homogenization ...
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bioRxiv - Bioengineering 2024Quote: ... The media was removed and 150 μL of serum free RPMI containing 0.25 mg/mL Liberase TL (Roche 054010120001) were added to the samples ...
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bioRxiv - Molecular Biology 2023Quote: ... thrombus samples were obtained and homogenized with 0.3 ml of PBS (pH 7.2) containing Complete Protease Inhibitor Mixture (Roche Diagnostics). The homogenates were centrifuged at 12,000 g for 15 min ...
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bioRxiv - Biochemistry 2023Quote: ... The cell pellet was resuspended in 300 µL of buffer containing 10 mM Na-phosphate buffer (pH 7.0) and EDTA-free Protease inhibitor (Roche). Soluble and membrane fractions were isolated by lysing cells with three freeze-thaw cycles (−20°C to room temperature) ...
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bioRxiv - Cancer Biology 2023Quote: ... Bones were cut into small fragments using scissors in a dissociating media containing 10 U/ml DNase I and Collagenase P (1.2 mg/ml, 11213865001, Roche). Bones and BM were digested at 37°C for 20 min under agitation ...
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bioRxiv - Cell Biology 2023Quote: ... washed once in PBS and lysed for 15–20 min on ice with lysis buffer A (50 mM Tris, pH 7.8, 1% NP-40, 150 mM NaCl) containing protease inhibitors (Complete, Roche), 2-mercaptoethanol ...
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bioRxiv - Biophysics 2024Quote: ... were resuspended in 20 mM Tris (pH 7.4) and 300 mM NaCl (50 mL volume) containing complete protease inhibitor cocktail (Roche) and lysed by soni- cation ...
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bioRxiv - Plant Biology 2024Quote: ... the tissue was finely chopped on ice for approximately 1 minute using 600 μL of pre-chilled Nuclei Isolation Buffer containing 0.4U/μL RNase inhibitor (Roche, Protector RNase Inhibitor ...
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bioRxiv - Immunology 2024Quote: ... The content was transferred to a 50 ml tube containing 2.5 ml of the same medium to which 0.5 mg/ml of collagenase A (Roche, 10103578001) and 0.01% DNase I (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoprecipitates were washed three times with lysis buffer containing 1x cocktail of protease inhibitors from Roche (Complete, EDTA free) and were then transferred to new tubes ...
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bioRxiv - Cancer Biology 2024Quote: Whole cell lysates were collected using RIPA lysis buffer (Beyotime Biotechnology, Jiangsu, China) containing 1% protease inhibitor (Roche, 11697498001) and 1% phosphatase inhibitor (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were lysed in SDS based lysis buffer (50 mM Tris Ph7.4, 150 mM NaCl, 1% SDS, 1mM EDTA and 50mM NaF) containing protease inhibitors (Roche) and sonicated ...
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bioRxiv - Molecular Biology 2024Quote: ... The pellet was resuspended with 1 ml of single nuclei wash buffer containing 0.2 U/µl RNase inhibitor (3335402001, Roche) and transferred to a microcentrifuge tube ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Both forebrains and connective tissues were dissociated in the solution containing 2mg/ml Collagenase/Dispase (Roche, Basel, Switzerland; 10269638001) in Hanks’ Balanced Salt solution ...
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bioRxiv - Cancer Biology 2024Quote: Cell lysates were prepared using NP-40 lysis buffer (100 mM Tris pH 8.0, 1% NP-40, 150mM NaCl) containing protease inhibitor cocktail (Roche) for 20 min with shaking on ice ...
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bioRxiv - Developmental Biology 2024Quote: ... Larvae were placed in 1.5mL microcentrifuge tubes containing 1mL of 5% cOmpleteTM EDTA-free protease inhibitor cocktail (PIC) (Roche Diagnostics Corporation ...
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bioRxiv - Immunology 2024Quote: Bacterial loads were evaluated by mechanical disruption of organs in 0.05% v/v Tween 20 in PBS containing a proteinase inhibitor cocktail (Roche) prepared according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The pellets were gently resuspended in 50 mM HEPES containing complete EDTA-free protease inhibitor mixture (Roche Applied Science) followed by cell disruption ...
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bioRxiv - Pathology 2020Quote: ... RNA was extracted with the High Pure Kit Nucleic Acid Kit DNA-RNA (Roche Diagnostics) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2022Quote: ... Illumina sequencing libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase Kit (Roche) and sequenced with 2×150bp paired-end sequencing on an Illumina NovaSeq6000 System ...
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Neural circuit-wide analysis of gene expression during deafening-induced destabilization of birdsongbioRxiv - Neuroscience 2022Quote: ... and library concentrations were determined using the KAPA Library Quantification Kit (Illumina Complete Kit, Roche). Samples were pooled at equal concentrations then size selected using a BluePippin and 2% BluePippin gels ...
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bioRxiv - Genetics 2023Quote: ... Completed libraries were quantified by qPCR using KAPA Library Quantification kit (Complete kit; Universal) (Roche) and sequenced with 150 bp paired-end reads on an Illumina NextSeq 550 instrument ...
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bioRxiv - Microbiology 2022Quote: ... A 2-step qPCR was done using the LightCycler 480 (Roche, Anderlecht, Belgium). The activation cycle was at 95 °C for 10 minutes ...
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bioRxiv - Genomics 2019Quote: ... 5.6mmol/L glucose with 2% BSA fraction V fatty acid free (Roche Diagnostics), 50μmol/L 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2019Quote: ... The enzymatic mix was composed by 2 μg/ml collagenase A (Roche 10103586001), 2,4 U/ml dispase II (Roche 04942078001 ...
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bioRxiv - Microbiology 2019Quote: ... Reactions were performed in 1X FastStart Universal Probe Master Mix (Rox) 2× (Roche) in 20 µl volume ...
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bioRxiv - Bioengineering 2020Quote: ... and 10 μl of 2× KAPA HiFi HotStart Ready Mix (KAPA Biosystems, USA). The following conditions were used ...
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bioRxiv - Genomics 2021Quote: ... ChIP enrichments were confirmed by qPCR with 2× SYBR FAST mastermix (KAPA Biosystems) using the CFX384 Real-Time System C1000 Touch Thermo Cycler (BioRad) ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM TCEP and an EDTA-free protease inhibitor cocktail tablet (cOmplete, Roche)) and lysed by sonication ...