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Citations for Roche :
2901 - 2950 of 9048 citations for Cow WAP kazal immunoglobulin kunitz and NTR domain containing protein 2 WFIKKN2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... whole testes from mice at postnatal day 21(P21) were lysed in RIPA buffer containing protease-inhibitor cocktail (11697498001, Roche). Protein lysates were then blocked by incubation with protein A Dynabeads (10002D ...
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bioRxiv - Microbiology 2022Quote: ... the V4 region of the 16S rRNA gene was amplified with barcode primers containing the index sequences using a KAPA HiFi HotStart Real-time PCR Master Mix (Roche). We monitored PCR product amplification and concentration on a Bio-Rad CFT Connect Real-Time PCR system ...
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bioRxiv - Genetics 2022Quote: ... with 5mM EDTA to remove waste and epithelial cells then were minced and dissociated in RPMI containing collagenase (100 mg/ml collagenase A; Roche), DNase I (50 μg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... At the completion of the assay islets were lysed in RIPA buffer (Pierce) containing 1 mM PMSF and EDTA-free protease inhibitor cocktail (Roche)) at 95°C for 5 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... The cell pellet was resuspended in buffer A (20mM Sodium Phosphate pH 7.4, 300mM NaCl, 30mM Imidazole) containing cOmplete™ protease inhibitor cocktail (Roche) and sonicated ...
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bioRxiv - Molecular Biology 2022Quote: Mouse pancreata from adult mice (9-14 week old) were perfused with a solution containing 1 mg/mL collagenase-P (Roche) in Hank’s buffered salt solution (HBSS ...
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bioRxiv - Cancer Biology 2022Quote: ... Wells were then washed twice with PBT 0.2% and blocked with PBT 0.2% containing 5% bovine serum albumin (Roche, Cat# 10735094001). Primary antibody staining with anti-Wg (1:500 ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.05% Tween 20) before exchange of the pre-incubation solution for hybridization buffer containing 3 ng/µl of digoxigenin-labeled (Roche) riboprobe ...
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bioRxiv - Developmental Biology 2022Quote: ... Matrigel drops (at least 4 samples per time point) were first digested with HBSS solution containing 10 U/ml dispase II (Roche) and 125 U/ml collagenase type IV (Gibco ...
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bioRxiv - Cell Biology 2022Quote: Whole endothelial extracts were prepared from 70-80% confluent cells in lysis buffer (3% SDS, 60 mM sucrose, 65 mM Tris (pH 6.8) containing protease inhibitor cocktail (Roche-11836170001). Protein extracts were sonicated briefly and the concentration of the isolated proteins was assessed using BCA Protein Assay Reagent (Thermo Scientific™ ...
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bioRxiv - Immunology 2022Quote: ... Single cell suspension was generated by mechanically disrupting tissues through a 100µm filter into FBS followed by a Percoll gradient centrifugation (40% Percoll, GE Healthcare; FBS free media containing 0.5mg/ml DNase, Roche). Cells were then washed with complete media and analyzed by flow cytometry.
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bioRxiv - Immunology 2022Quote: ... Tissue was incubated at 37°C for 15 minutes in FBS free DMEM media containing Liberase™ TL (0.25mg/ml; Roche) and DNAse I (0.5mg/ml ...
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bioRxiv - Immunology 2022Quote: ... Tissue was incubated at 37°C for 30 minutes in FBS free DMEM media containing Liberase™ TL (0.25mg/ml; Roche) and DNAse I (0.5mg/ml ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were lysed by the lysis buffer 10 M urea containing the cOmplete™ protease inhibitor cocktail (Roche, #11697498001). The cell tubes were ultrasonicated at 4 ℃ for two cycle (1 min per cycle ...
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bioRxiv - Genomics 2022Quote: Cells were lysed in RIPA buffer (Beyotime, China) containing protease inhibitors (Complete Mini Ethylene Diamine Tetraacetic Acid-Free Protease Inhibitor Cocktail, Roche). The protein lysates were centrifuged ...
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bioRxiv - Immunology 2022Quote: ... tumor tissue was incubated at 37°C for 30minutes in FBS free DMEM media containing liberase™ TL (0.25mg/ml; Roche) and DNAse I (0.5mg/ml ...
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bioRxiv - Genetics 2022Quote: ... 50000 cells were lysed in NPB buffer (PBS containing 5% BSA, 1mM DTT, 0.2% IGEPAL, 1□×_Roche Complete Protease Inhibitor) and incubated on ice for 15 min to isolate nuclei ...
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bioRxiv - Neuroscience 2022Quote: ... washing them twice with PBS containing 1 mM CaCl2 and MgCl2 and collecting cells by scraping them in CaCl2/MgCl2 PBS containing cOmplete protease inhibitor cocktail (Roche). Lysates were cleared by centrifuging at 1000 x g for 10 min at 4 °C and pellets were resuspended in PBS with 1 % Igepal ...
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bioRxiv - Microbiology 2022Quote: ... 33.75 µL cells were mixed with a PCR master mix containing 37.5 µL KAPA HiFi HotStart ReadyMix (Roche, CN KK2602), 2.25 µL second strand synthesis primer SMRT_dT (10 µM ...
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bioRxiv - Molecular Biology 2022Quote: ... Tissue fragments were dissected with sterile scissors in a FBS-free DMEM solution containing 1x Liberase TM Research Grade (Roche) and Antibiotic-Antimycotic (Gibco) ...
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bioRxiv - Neuroscience 2022Quote: Cells were washed twice with PBS and scraped in the according lysis buffer containing 1x cOmplete protease inhibitor cocktail (PI) (Roche) on ice ...
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bioRxiv - Molecular Biology 2023Quote: HEK293 tauRD biosensor cell pellets were lysed by resuspension in cold 0.01% Triton in PBS containing cOmplete™ mini EDTA-free protease inhibitor tablet (Roche) at a concentration of 10% w/vol followed by incubation in the lysis buffer for 15 minutes on ice ...
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bioRxiv - Molecular Biology 2023Quote: ... and DS10 cell pellets were lysed by resuspension in cold 0.05% Triton in PBS containing cOmplete™ mini EDTA-free protease inhibitor tablet (Roche) at a concentration of 10% w/vol followed by incubation in the lysis buffer for 20 minutes on ice ...
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bioRxiv - Molecular Biology 2023Quote: ... and each was lysed by trituration in 1 mL ice-cold PBS with 0.25% Triton-X containing cOmplete™ mini EDTA-free protease inhibitor cocktail tablet (Roche) at a concentration of 10% w/vol followed by a 15-minute incubation on ice ...
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bioRxiv - Molecular Biology 2022Quote: ... The dtSARM1 was released from the cytoplasm by incubating with ice-cold PBS containing 200 μM Digitonin and protease inhibitor (Roche).The cleared lysate was incubated with StrepTactinTM resin (IBA ...
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bioRxiv - Neuroscience 2022Quote: ... Pulverized cortex and hippocampal samples were homogenized on ice using a pestle pellet motor in 10X volume of homogenization buffer containing PhosphoSTOP (Roche) and cOmplete Mini Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... cell pellets were resuspended in 400 µL Equilibration Buffer A containing protease-inhibitor mix (Complete EDTA-free; 11 873 580 001, Roche). Cells were lysed using the equivalent of 100 µL glass beads and 4 cycles of 30-second on/off vortexing (incubating on ice during the “off” cycle) ...
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bioRxiv - Molecular Biology 2022Quote: ... cell pellets were resuspended in 1 mL Equilibration Buffer B containing protease-inhibitor mix (Complete EDTA-free; 11 873 580 001, Roche) and 0.2 mg/mL lysozyme ...
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bioRxiv - Cancer Biology 2024Quote: ... human 293FT cells were grown on 10cm dishes and transfected with sgRNA-containing vector (pX330A-1x2-cNAT10/PITCh) and donor vector using Xtremegene 9 transfection reagent (Roche). 72 h after transfection ...
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bioRxiv - Plant Biology 2024Quote: ... The transformants were screened on MS plants with 1% sucrose and 0.8% agar containing 40mg/L hygromycin (Roche, no. 10843555001). For each construct ...
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bioRxiv - Bioengineering 2024Quote: ... the supernatant was discarded, and 5 mL of Hank’s Balanced Salt Solution (Ca-, Mg-) (gibco) buffer containing liberase (Roche, #05401119001) (50 mg/mL ...
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bioRxiv - Neuroscience 2024Quote: hiPSC-DANs cell pellets were collected in ice-cold PBS and were homogenised in RIPA buffer containing protease (cOmplete EDTA-free, Roche) and phosphatase inhibitors (PhosSTOP ...
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bioRxiv - Molecular Biology 2024Quote: ... Pellets were resuspended in 15 ml of Lysis buffer (50 mM Tris HCl pH 7.5, 500 mM NaCl) containing Complete EDTA-free protease inhibitor (Roche, 11873580001), and sonicated 10 cycles of 15 seconds on ...
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bioRxiv - Molecular Biology 2024Quote: ... Ovaries were cut into 6-8 pieces and enzymatically digested in L-15 media containing Liberase TM (Roche, Indianapolis, IN) and DNase I (Worthington Biochemicals ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were minced by mechanical chopping and incubated in 1xPBS containing 150 mg/mL DNAse I (Roche Diagnostics, Indianapolis, IN) and 25 mg/mL liberase (Roche Diagnostics ...
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bioRxiv - Microbiology 2024Quote: ... The bacterial pellets were then resuspended in 20 ml of lysis buffer pH 8.0 (50 mM NaH2PO4, 300 mM NaCl, 5 mM Imidazole) containing 1× cOmplete EDTA-free protease inhibitor cocktail (Roche, Germany) and 1 mg/ml lysozyme ...
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bioRxiv - Cell Biology 2023Quote: Frozen liver tissues that were crushed using a Multi-beads Shocker (Yasui Kikai, Japan) or cultured cells were dissolved in RIPA buffer containing 1× cOmplete proteinase Inhibitor Cocktail (Roche). Protein concentration in the lysates was determined using a BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We did PCRs in a reaction volume of 25 μl containing 12.5 μl 2x FastStart PCR master mix (Roche, Germany), one forward and two reverse MHC IIB primers (Bracamonte et al. ...
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bioRxiv - Cell Biology 2024Quote: ... 100 synchronized young adult worms (24h post-L4 stage) were picked into 30 μl of 1XTE (Tris-EDTA Buffer, pH=8) containing 1X complete protease inhibitor cocktail (Roche) and flash-frozen in liquid nitrogen ...
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bioRxiv - Immunology 2024Quote: ... Lungs were resected and minced with scissors before incubation in a collagenase buffer containing 1 mg/mL Collagenase-A (Roche), 2000U DNaseI (Sigma) ...
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bioRxiv - Cell Biology 2024Quote: ... followed by an 8-minute perfusion with myocyte digestion buffer containing 5 mg of liberase dispase high DH enzyme (Roche) per digestion ...
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bioRxiv - Immunology 2023Quote: ... Hemolymph was extracted by proboscis clipping from groups of 40 mosquitoes per genotype in sterile 1x phosphate-buffered saline (PBS) containing a protease inhibitor cocktail (Roche), and protein concentration determined using Bradford protein quantification assay (Fermentas) ...
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bioRxiv - Immunology 2024Quote: ... All lung lobes were cut into small pieces and digested in R5 media (RPMI 1640 supplemented with FCS (5 %) containing DNase I grade II (30 µg/ml) and Liberase TM (50 µg/ml, Roche) for 45 min at 37 °C with a magnetic stirrer.
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bioRxiv - Biochemistry 2022Quote: Cells were washed and harvested in PBS 1x, lysed in RIPA buffer 1x (Thermo, 88900) containing phosphatase and protease inhibitors (Roche). Lysates were centrifuged at 15,000 g to separate soluble and pellet fractions ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed once with PBS and resuspend with Tris-HCl containing 4 mM EDTA and 1× complete protease inhibitor cocktail (Roche). Dounce homogenizer is used to fractionate the cell membranes and then 3 min 600 g centrifugation was used to obtain Post-Nuclear Supernatants (PNS) ...
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bioRxiv - Immunology 2022Quote: ... frozen tissue fragments were thawed and incubated for 5 minutes at 37°C in a dissociation buffer containing RPMI-1640 supplemented with 10 U/mL DNase (Roche), 200 U/mL collagenase IV (Worthington Biochemical) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Slides were incubated with 50 μL of block buffer containing a fluorescein-labeled anti-DIG antibody (sheep, 1:100, Roche) for 60 min at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... For subsequent PCR amplification of the cDNA the optimal PCR cycle number was determined with an aliquot of 1 μL unpurified cDNA in a 10 μL qPCR containing 1X Kapa HiFi Hotstart Readymix (Roche), 1x SybrGreen and 0.2 μM UP primer (Table S2) ...
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bioRxiv - Plant Biology 2023Quote: ... untreated and chitosan treated tissue samples were ground in liquid nitrogen and dissolved in an extraction buffer containing Phosstop (Roche), and the concentration of proteins determined by the Bio-Rad protein assay kit with bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: ... IPTG-treated bacteria were washed with PBS and eluted with 1X HEPES buffer containing 1% Triton X-100 and EDTA-free protease inhibitor cocktail (Roche) The elutes were sonicated and centrifuged at 18,000 x g ...