Labshake search
Citations for Roche :
2851 - 2900 of 9048 citations for Cow WAP kazal immunoglobulin kunitz and NTR domain containing protein 2 WFIKKN2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... The washed pellet was resuspended in approximately 1/3 of packed cell volume of ORC lysis buffer containing cOmplete Protease Inhibitor Cocktail Tablet (1 tablet per 25 mL total volume; Roche) and frozen dropwise in liquid nitrogen.
-
bioRxiv - Neuroscience 2020Quote: ... Cells were dissociated into a single-cell suspension by incubating the retinas in 1mL of HBSS containing 10U of papain (Roche) and 200µM cysteine ...
-
bioRxiv - Neuroscience 2021Quote: ... Tissue was thawed on ice and homogenized using a motorized pestle in RIPA buffer containing both protease and phosphatase inhibitor cocktails (Roche). Lysis was completed for 15 minutes on ice ...
-
bioRxiv - Biophysics 2021Quote: ... Cells were washed with ice-cold PBS and resuspended in Tris-HCl containing 4mM EDTA and 1X Complete protease inhibitor cocktail (Roche). Every step was then performed at 4° C ...
-
bioRxiv - Neuroscience 2021Quote: ... were either left empty or filled with 6 μl of a mix of post-translational modification (PTM) inhibitors containing the following reagents: phosphatase inhibitors (PhosSTOP, 04906845001, Roche), protease inhibitors (cOmplete Protease Inhibitor Cocktail ...
-
bioRxiv - Neuroscience 2022Quote: ... Hippocampi were isolated and stored in 10 volumes of sucrose buffer 1 (320mM sucrose, 1mM NaHCO3, 1mM MgCl2, 0.5mM CaCl2, 1µM PMSF) containing protease inhibitor cocktail (Roche, Mannheim, Germany). Tissues were homogenized for 10 strokes using a motor-driven 2 ml Potter-Elvehjem homogenizer fitted with a Teflon pestle ...
-
bioRxiv - Developmental Biology 2022Quote: ... The liver was dissected from the abdominal cavity and placed into a solution containing Hanks’ buffered saline solution (HBSS) supplemented with 1.07 Wünsch units/ml Liberase DH (Roche Applied Science) and 70 U/ml hyaluronidase (Sigma-Aldrich) ...
-
bioRxiv - Genomics 2022Quote: Human embryonic hindlimb muscles were collected in sterile PBS and mechanically dissociated using fine scissors in 10 mL (per gram of tissue) of enzyme solution containing 2.5 U/mL Dispase II (Roche, #4942078001) and 1 mg/mL Collagenase B (Roche ...
-
bioRxiv - Neuroscience 2019Quote: ... cells were washed with cold PBS and lysed on ice with RIPA buffer containing complete mini protease inhibitor cocktail (Roche), followed by bath ultrasonication in chill water and centrifugation for total protein extract ...
-
bioRxiv - Neuroscience 2019Quote: ... Remaining pellets were washed with PBS and resuspended in 1% sarkosyl in PBS containing complete mini protease inhibitor cocktail (Roche), vortexed for 30 sec and incubated at room temperature for 1 hr ...
-
bioRxiv - Cell Biology 2019Quote: Lysates from A375 cells stably expressing FLAG-EZH2 with or without Dox-inducible expression of FLAG-USP7 were prepared by incubating the cells in lysis buffer containing protease inhibitor cocktail (Roche). Anti-FLAG immunoaffinity columns were prepared using anti-FLAG M2 affinity gel (Sigma ...
-
bioRxiv - Genetics 2019Quote: Protein was isolated from 10 brain-imaginal disc complexes were dissected from wandering L3 larvae or S2 cells and homogenized in 1X Laemmli/RIPA buffer containing 1X protease inhibitors (Roche cOmplete Mini EDTA-free protease inhibitor tablets ...
-
bioRxiv - Developmental Biology 2019Quote: ... testis lysates were prepared by sonication of ∼100-mg pieces of freshly removed whole testis in 1.0 mL ice-cold RIPA buffer containing protease inhibitors (cOmpleteTM, EDTA-free Protease Inhibitor Cocktail Tablets, Roche, 4693159001) and phosphatase inhibitors (PhosSTOPTM ...
-
bioRxiv - Microbiology 2019Quote: Whole cell lysates were prepared by suspending purified parasite pellets in whole cell lysis buffer (1XPBS, 1% v/v Triton X-100) containing Protease Inhibitors (cOmplete EDTA-free, Roche). 24 h in vitro ookinetes were also subjected to cellular fractionation using the following method ...
-
bioRxiv - Molecular Biology 2020Quote: MtDNA copy number was analyzed in duplicate by quantitative real-time PCR using 2 μl of 1/400-diluted SacI-treated total DNA in a 10 μl reaction containing 0.2 μM forward and reverse primers and 1× KAPA SYBR FAST qPCR Master Mix for LightCycler 480 (KAPA Biosystems) in a LightCycler 96 instrument (Roche) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Transfected cells were harvested in ice-cold PBS using a cell scraper and lysed by sonication for three times 10 seconds in ice-cold PBS containing Complete protease inhibitors (Roche). Unbroken cells were removed by centrifugation at 500 g for 2 minutes ...
-
bioRxiv - Microbiology 2020Quote: ... Hemolymph was collected from cold anesthetized mosquitoes by the proboscis clipping method directly into 1X PBS containing 2X EDTA-free cOmplete Mini Protease Inhibitor Cocktail (Roche). Hemolymph protein concentrations were determined using a modified Bradford assay (Bio-Rad ...
-
bioRxiv - Developmental Biology 2019Quote: Three to five-day-old female ovaries were dissected and homogenized in RIPA lysis buffer containing protease and phosphatase inhibitors (Roche). In some samples ...
-
bioRxiv - Molecular Biology 2019Quote: ... cell culture media supernatants were collected and RNA lysis buffer (Fenozol Plus, A&A Biotechnology, Poland) or RIPA buffer containing protease inhibitors (Roche) were added to the specified wells ...
-
bioRxiv - Plant Biology 2020Quote: ... in a reaction volume of 20 μL containing 10 μL of the LightCycler 480 SYBR Green I Master Mix (Roche), 0.3 μM of each specific forward and reverse primer (Table S9 ...
-
bioRxiv - Immunology 2020Quote: ... intestines were further chopped and incubated for 30 min at 37°C in medium containing liberase (20 µg/ml, Roche) and DNAse (400 µg/ml ...
-
bioRxiv - Synthetic Biology 2021Quote: The cell pellet was thawed and resuspended in lysis buffer (1×PBS containing 150 mM NaCl, pH 7.4, 0.5 mM TCEP, 0.1 mM PMSF, 1 × Roche complete protease inhibitors), and lysed in a cell disruptor ...
-
bioRxiv - Microbiology 2020Quote: After adding 1 mL of PBS (pH 7.4) containing protease inhibitor (1 tablet per 10 mL, Roche cOmplete™, #04693159001) and 500 μL of 0.5 mm diameter glass beads ...
-
bioRxiv - Zoology 2020Quote: ... the digoxygenin probe is visualized by replacing the APB is replaced with the same containing 20 µl/ml of a commercial NBT/BCIP stock solution (Roche Diagnostics GmBH ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: Whole-cell lysates were prepared by using Triton-X sample buffer containing protease inhibitor cocktail from Roche (Grenzach-Wyhlen, Germany). The protein concentration was assessed by using the DC Protein assay reagent obtained from Bio-Rad Laboratories ...
-
bioRxiv - Genetics 2021Quote: ... cells were collected in lysis buffer (TBS + 1% Triton X-100) containing protease inhibitors (Complete Mini EDTA-Free, Roche 04693159001), briefly sonicated ...
-
bioRxiv - Immunology 2020Quote: Cell lysates from flow-sorted bone marrow osteoclast progenitors grown in presence of M-CSF and RANKL over time were harvested in 1% NP40 lysis buffer containing 1X cOmplete Protease Inhibitor cocktail (Roche). Samples were separated by SDS-PAGE and transferred to nitrocellulose membrane ...
-
bioRxiv - Microbiology 2021Quote: ... the parasites were re-suspended in 1xPBS containing 1 mg/ml soybean trypsin inhibitor and 25x protease inhibitor cocktail (Roche) before the parasites were pelleted and lysed in lysis buffer (4% SDS ...
-
bioRxiv - Biochemistry 2020Quote: ... 2.5 mL of Sf9 cells at a density of 1.2×106 cells/mL were transfected with 5 µL of recombinant bacmid containing the gene of interest using 3 µL of X-tremeGENE HP DNA transfection reagent (Roche) and 100 µL of transfection medium (Expression Systems) ...
-
bioRxiv - Biochemistry 2019Quote: ... The lens-vitreous tissue was then placed into a filtered centrifugation tube containing 20 microliters of protease inhibitor cocktail (Roche) dissolved in PBS ...
-
bioRxiv - Immunology 2021Quote: ... the dura mater was carefully removed from the brain and then chopped for enzymatic digestion in Hanks’ Balanced Salt Solution (HBSS) containing 15 mg/mL collagenase D (Roche) and 1 mg/mL DNase (Roche ...
-
bioRxiv - Biochemistry 2020Quote: Cells were washed twice with ice-cold PBS and lysis in ice-cold 100 mM sodium carbonate containing protease inhibitor cocktail (Roche) by tip-probe sonication ...
-
bioRxiv - Neuroscience 2020Quote: ... Protein aggregation was evaluated by western blot in total cell extracts prepared in 1% Triton X-100 in PBS containing proteases and phosphatases inhibitors (Roche). Sample quantification was performed with the Pierce BCA Protein Assay Kit (Thermo Scientific).
-
bioRxiv - Cell Biology 2020Quote: ... Cells were harvested by centrifugation and lysed in 1% Nonidet P40/1 mM EDTA/PBS containing Complete protease inhibitors (Roche), and nuclei were removed by centrifugation ...
-
bioRxiv - Biochemistry 2021Quote: The retina was homogenized in lysis buffer (100 mM Tris-HCl [pH 7.4], 300 mM sucrose) containing a 1X cOmplete protease inhibitor cocktail (Roche, Switzerland). After removing the tissue debris (800 × g ...
-
bioRxiv - Biochemistry 2021Quote: ... retinas were homogenized in lysis buffer (100 mM Tris-HCl [pH 7.4], 300 mM sucrose) containing a 1X cOmplete protease inhibitor cocktail (Roche, Switzerland). After removing the tissue debris (800 × g ...
-
bioRxiv - Neuroscience 2021Quote: ... tissue was homogenized by probe sonication at 20kHz for 10s in ice-cold TBS buffer (tris-buffered saline, 1% Triton X-100; pH 7.4) containing protease inhibitor (Roche 11697498001) and phosphatase inhibitor (Sigma 4906845001 ...
-
bioRxiv - Neuroscience 2021Quote: Protein lysates were prepared following euthanasia of zebrafish larvae (6 dpf) in RIPA buffer containing protease inhibitors (Complete ULTRA Tablets, Roche), followed by homogenisation via probe sonication ...
-
bioRxiv - Genomics 2022Quote: ... The reaction was quenched using 125 mM glycine for 5 minutes with rotation and the cells were washed three times using ice-cold PBS containing complete protease inhibitor cocktail tablets (Roche). Cells were resuspended in lysis buffer (1% SDS ...
-
bioRxiv - Neuroscience 2021Quote: Dissected brain regions of interest or culture samples were homogenized with TX-soluble buffer (50 mM Tris [pH 8.0], 150 mM NaCl, 1% Triton-X 100) containing protease and phosphatase inhibitors (Roche, USA). The supernatants were collected for soluble fraction after centrifugation (20 ...
-
bioRxiv - Developmental Biology 2021Quote: ... The testicular tissue was finely minced and incubated in a dissociating solution containing 40 mg/mL Collagenase H (Roche, Switzerland), 33.3 mg/mL Dispase II (Roche) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Tumors were digested in 500 µL (STAMP microtumors) or 3000 mL (subcutaneous) of phosphate buffered saline containing 0.1 mg/mL DNAse I (Roche, Switzerland) and collagenase D at 1 mg/mL (Roche ...
-
bioRxiv - Cancer Biology 2020Quote: ... minced with a sterile scalpel in a petri-dish containing complete medium (DMEM/10%FBS/2mM L-Glutamine/1x NEAA/1x Sodium Bicarbonate/10mM HEPES/ 1x Pen/Strep) and then incubated with complete medium containing 1 mg/ml Collagenase P (Roche) for 90 minutes at 37°C ...
-
bioRxiv - Developmental Biology 2021Quote: ... 9 and 12h node induced and contralateral uninduced tissues were dissected in ice cold 1x PBS and transferred to low binding PCR tubes containing 1x Protease Inhibitor Cocktail in 1xPBS (cOmplete mini EDTA-free; Roche) on ice ...
-
bioRxiv - Cancer Biology 2021Quote: ... tumors were mechanically minced and incubated for 30-45 minutes in DMEM/Ham’s F12 medium (Fujifilm, 042-30555) containing Liberase DH (Roche, 5401089001) at a final concentration of 0.26 U/mL at 37°C with continuous stirring ...
-
bioRxiv - Cell Biology 2020Quote: ... followed by addition of 300 μL of RIPA buffer containing cOmplete™ protease inhibitor and phosphatase inhibitor (both from Roche). Cells were scraped with cell scrapers and transferred to 1.5 tubes ...
-
bioRxiv - Immunology 2020Quote: ... Bacterial pellets obtained by centrifugation at 13,500g for 10 min at 4°C were resuspended in 1 ml of PBS containing protease inhibitors (cOmplete Mini, Roche; 1 tablet per 10 ml of PBS following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... HEK293T and A549 cells were lysed in ice-cold RIPA lysis buffer containing complete protease inhibitor and phosphatase inhibitor cocktails (Roche), resolved by SDS-PAGE ...
-
bioRxiv - Immunology 2020Quote: ... mouse thymus was collected and homogenized in 1 ml of cold PBS containing cOmplete protease inhibitor cocktail (Roche, Indianapolis, IN) using a tissue homogenizer (Omni International ...
-
bioRxiv - Cell Biology 2021Quote: ... The cell pellets were thawed on ice and lysed (3 freeze thaw cycles) after re-suspending each one in 100 μl of PBS containing protease inhibitors (Roche), 0.8 % NP40 ...