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Citations for Roche :
2501 - 2550 of 9048 citations for Cow WAP kazal immunoglobulin kunitz and NTR domain containing protein 2 WFIKKN2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... 5 ul of the three amplicons with 20 μl of PCR master mix containing 1.25X KAPA HiFi HotStart ReadyMix (Roche), 0.375 uM of the i5 primer (Microsynth AG ...
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bioRxiv - Systems Biology 2020Quote: ... Supernatants were discarded and protein resuspended in Guanidine-HCl buffer (6M Gnd-HCl, 50mM Tris-HCl, pH 8.5, 5mM NaF, 5mM beta-glycerophosphate, 1mM Na-orthovanadate, containing Roche complete protease inhibitor ...
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bioRxiv - Neuroscience 2019Quote: ... The brains were transferred to an 1.5 ml Eppendorf tube containing 500 microliters 1 mg/ml Liberase DH (Roche, prepared according to the manufacturer’s recommendation ...
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bioRxiv - Immunology 2019Quote: ... MLN from indicated groups of mice were dissected into small pieces and digested with freshly made enzyme mix comprised of RPMI-1640 containing 0.12 mg/ml Collagenase P (Roche), 0.4 mg/ml Dispase (Roche ...
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bioRxiv - Immunology 2019Quote: ... containing phosphatase inhibitors (1 mM NaF and 0.1 mM Na3VO4) and protease inhibitors (cOmplete mini cocktail, EDTA-free, Roche). Immunoblots were carried out using the following antibodies ...
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bioRxiv - Molecular Biology 2019Quote: ... 5–10 × 106 HEK-293 or MEF cells were washed with cold sucrose-containing solution I (0.5 M sucrose, 3 mM MgCl2 with Cocktail protease inhibitor, Roche), harvested by scraping into 1 ml solution I and sonicated in a BioRuptor for 10 cycles (15 sec ON/15 sec OFF) ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then resuspended in 10 mM Tris-HCl buffer (pH 7.4) containing protease inhibitor cocktail (cOmplete Tablets, EDTA-free, Roche) after which cell lysis was performed with glass beads (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: Parasite were isolated from rings and early trophozoite stages and lysates were prepared in 1X PBS containing 0.5% TritonX-100 and protease inhibitors (Roche) by sonication (amplitude 30 ...
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bioRxiv - Cell Biology 2019Quote: HeLa cells were lysed in 1% NP-40 lysis buffer (150 mM NaCl, 20 mM HEPES, 0.5 mM EDTA) containing complete protease and phosphatase inhibitor cocktail (Roche). 10 mg of total protein lysate were incubated for 3 h with protein A Dynabeads (Invitrogen ...
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bioRxiv - Genetics 2019Quote: Tissues from 15-30 animals were dissected in CST lysis buffer (Cat#9803) containing 2x protease inhibitor (Roche, 04693159001) and 3x phosphatase inhibitor (Roche ...
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bioRxiv - Developmental Biology 2019Quote: ... 1X Denhardt solution) containing 1μg/ml DIG labeled RNA probes (prepared with a DIG RNA labeling mix, Roche, 11277073910) for overnight at 65°C in a humid chamber ...
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bioRxiv - Microbiology 2020Quote: ... and pellets were disrupted by sonication in PBS buffer (pH 8.0) containing 10 mM EDTA as well as protease inhibitors (Roche) and ...
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bioRxiv - Immunology 2021Quote: ... coated with H-2Kb-OVA and ICAM-1 at a 1:1 ratio for various times at 37 ºC and immediately lysed in 2× cold lysis buffer containing phosphatase inhibitors (1 mM NaF and 0.1 mM Na3VO4) and protease inhibitors (cOmplete mini cocktail, EDTA-free, Roche). Activation of PI3K and MAP kinase signaling was assessed by immunoblot for pAkt (Phospho-Akt (Ser473 ...
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bioRxiv - Immunology 2021Quote: Single-cells were sorted into PCR tubes containing 1μl of cell lysis solution (1:10 Cell Lysis buffer(Roche), 10U/μl Rnasin plus Ribonuclease inhibitor (Promega ...
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bioRxiv - Neuroscience 2021Quote: ... samples were homogenized in a lysis buffer containing a mixture of protease inhibitor (Protease Inhibitor Cocktail Tablets - Roche Diagnostics). Proteins were separated using SDS-polyacrylamide gel electrophoresis (SDS-PAGE-4-12% ...
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Biochemical alterations precede neurobehavioral deficits in a novel mouse model of Friedreich ataxiabioRxiv - Biochemistry 2021Quote: ... All the pieces were placed in 1.5 ml screw cap polypropylene tubes in the presence of lysis buffer consisting in 50 mM tris(hydroxymethyl)aminomethane (Tris) HCl pH 7.5 containing a protease inhibitor cocktail (Roche). For each 100 mg of tissue ...
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bioRxiv - Biochemistry 2020Quote: ... Elution buffer (50 mM Na2HPO4, 0.3 M NaCl, 250 mM imidazole; pH 8) containing EDTA-free protease inhibitors (cOmplete, Roche) was used to elute the proteins ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were harvested and incubated in buffer A (50 mM MMT pH 8.0/300 mM KCl/10 mM MgCl2/5 % glycerol) containing 1 g L-1 lysozyme/2.5 U mL-1/SmDNAse//complete protease inhibitor cocktail (Roche) for 1 h prior to lysis using EmulsiFlex C5 (Avestin ...
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bioRxiv - Biochemistry 2020Quote: ... The medium was discarded and the cells were resuspended in 200 µL of ice-cold 50 mM HEPES buffer pH 7.4 containing 1 x protease inhibitor cocktail (Roche) and 1 mM phenylmethylsulfonyl fluoride ...
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bioRxiv - Biochemistry 2020Quote: ... Lacrimal glands were also harvested and homogenized in 0.2% Triton X-100 (MilliporeSigma) containing a protease inhibitor cocktail (Roche) and prepared for PRG4 western blotting (2.2) ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were lysed with 400 μL of NAD+/NADH extraction buffer containing a cocktail of protease inhibitor (Roche Diagnostic ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were lysed in 1% NP-40 lysis buffer (150 mM NaCl, 20 mM HEPES, 0.5 mM EDTA) containing complete protease and phosphatase inhibitor cocktail (Roche). Total cell extracts were incubated for 3 h with 20 μl protein A Dynabeads (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... The cells pellet was resuspended in Lysis buffer (20mM Tris-HCl pH 7.5, 150mM NaCl, 1mM EDTA) containing protease inhibitor cocktail (Roche) and lysed using mechanical disruption (Isobiotec Cell Homogenizer) ...
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bioRxiv - Biochemistry 2021Quote: ... Mitochondria were resuspended in SEM buffer (250 mM sucrose, 1 mM EDTA, 10 mM MOPS-KOH, pH 7.2, containing 1X protease inhibitor cocktail from Roche).
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bioRxiv - Cell Biology 2021Quote: ... then 10-15 ml of pre-warmed to 37 °C enzyme buffer solution (EBS) containing 0.4 mg/ml Protease (Roche) and then with 15-20 ml of 37 °C EBS containing collagenase D (0.1 U/ml ...
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bioRxiv - Cell Biology 2021Quote: ... 300ul lysis buffer (50mM Tris-HCl, 150mM NaCl, 0.5% NP40 and 1mM EDTA) containing protease inhibitor (Roche, Basel, Switzerland) and 0.2M PMSF was added to each well and incubated on ice for 15 minutes ...
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bioRxiv - Microbiology 2020Quote: Adherent macrophages in 6-well plates were washed with PBS containing 1 mM sodium orthovanadate and 10 mM 1,10-phenanthroline (Roche) on ice prior to lysis ...
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bioRxiv - Neuroscience 2020Quote: Retinas were lysed on ice for 10 min in lysis buffer (20mM HEPES, 150mM NaCl, 1.5mM NgCl2, 2mM EGTA with 0.5% TritonX-100) containing protease inhibitors (Roche #5892970001) and phosphatase inhibitors (10mM NaF ...
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bioRxiv - Microbiology 2021Quote: The AAV2 stock sample (1.5*1011 genome-containing particles) was pre-treated with 10U DNase I (04716728001, Roche, Switzerland) in 1x DNase buffer for 15 min at 37°C to remove any DNA outside the particles ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cultures were centrifuged at 700 g for 3 min to remove the media and parasites were isolated from the RBC by incubating with 0.1% saponin in PBS containing protease (Roche) and phosphatase inhibitors (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... RNA was isolated from 200 µl of virus-containing cell culture supernatants using TriPure isolation reagent (Roche Applied Science) and purified according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... pH 8 containing 150 mM NaCl, 1% Nonidet-P40, 0.1% SDS, 10 mM NaF, and EDTA-free protease inhibitor cocktail [Roche]). Cellular debris and bacteria were removed by centrifugation and collected as the bacterial fraction ...
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bioRxiv - Plant Biology 2021Quote: ... qPCR reactions were performed in 10 μl reactions containing 4 μl of LightCycler 480 SYBR Green I Master (Roche), 4 μl of PCR-grade water (Roche) ...
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bioRxiv - Cancer Biology 2020Quote: Cells were treated in lysis buffer (50 mM Tris-HCl, 0.5% sodium deoxycholate, 25 mM EDTA) containing protease inhibitor cocktail (Roche) for 30 min on ice ...
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bioRxiv - Cell Biology 2021Quote: ... After addition of the extraction solution containing 1% acetic acid and Complete Mini protease inhibitor cocktail (Roche, Basel, Switzerland) in 1:2 w/v proportion ...
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bioRxiv - Cell Biology 2020Quote: ... cells from mouse blood infected with the DEH-GFP-expressing parasite were pelleted and then lysed in hypotonic buffer (10 mM Tris-HCl pH 8.4, 5 mM EDTA) containing protease inhibitors (Roche), freeze/thawed twice ...
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bioRxiv - Cancer Biology 2021Quote: Cell pellets and tumor-bearing mammary glands were homogenized in 1% SDS solution containing protease (cat# 11836153001, Roche AG) and phosphatase inhibitor cocktail (cat# 04906845001 ...
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bioRxiv - Cell Biology 2021Quote: ... were harvested in ice-cold lysis buffer (0,25% NP40, 5% glycerol, 50 mM Tris HCl, 150 mM NaCl) containing protease inhibitor (Roche), DNAse (5 µl/ml ...
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bioRxiv - Cell Biology 2021Quote: ... The pelleted cells were homogenized in 1X RIPA lysis buffer containing freshly added phosphatase and protease inhibitor cocktail (Roche). To remove cellular debris ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumors were injected in multiple locations with a total of 1.5 mL RPMI containing 50 µg/mL Liberase DL research grade (Roche) and 10 U/mL DNase I (Sigma) ...
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bioRxiv - Developmental Biology 2022Quote: ... and then homogenized with a glass tissue homogenizer in lysis buffer (50 mM Tris pH 7.5, 150mM NaCl, 1% NP-40, 0.5% sodium deoxycholate) containing protease inhibitors (Roche, 11697498001). Supernatant fractions were collected by centrifugation and boiled with 2× Laemmli sample buffer (Bio-Rad ...
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bioRxiv - Biophysics 2022Quote: ... 0.3 M NaCl) containing either 200 µM PMSF or a tablet of complete EDTA-free protease-inhibitor cocktail (Roche) and 10 ng ml−1 DNAse I ...
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bioRxiv - Neuroscience 2022Quote: ... The human tissues were homogenized in TBS (20 mM Tris-HCl, 140 mM NaCl, pH 7.5) containing protease and phosphatase inhibitors (Roche). Homogenates were ultracentrifuged (4 °C for 60 min ...
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bioRxiv - Developmental Biology 2022Quote: ... The alkaline phosphatase activity was visualized by incubating the specimens in NTMT containing 0.07 mg/ml nitroblue-tetrazolium chloride (Roche), and 0.035 mg/ml 5-bromo-4-chloro-3-indolyphosphatase (Roche) ...
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bioRxiv - Molecular Biology 2022Quote: ... after which the cultures were treated with 0.15% saponin in PBS containing cOmplete Mini EDTA-free Protease and PhosSTOP Phosphatase inhibitor cocktails (both Roche) for 10 min at 4°C to lyse the host erythrocytes.
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bioRxiv - Immunology 2022Quote: ... Cells were spun down again and the pellet resuspended in 1.25 ml cold lysis buffer (PBS containing 1% TX-100, 1 mM NEM, and Complete Protease Inhibitor from Roche). Cells were lysed for 30 minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... The cells were lysed in TBS/1% (v/v) IGEPAL-630 (sigma) containing 1X Complete protease cocktail inhibitors (Roche). The TCR-CD3 complex was purified from the lysate using Dynabeads M-280 streptavidin (Invitrogen) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Lung segments were suspended in 10X volume of ice cold PBS containing cOmplete Mini protease inhibitor cocktail (Roche, Switzerland), 2mM EDTA (Sigma ...
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bioRxiv - Physiology 2022Quote: ... for 3 min and incubated twice for 3 min with CSK buffer containing 0.5% Triton X-100 and complete protease inhibitors cocktail (catalog no. 11873580001, Roche). Cells were after washed with PBS-S ...
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bioRxiv - Microbiology 2022Quote: ... bacteria were washed twice in phosphate buffer saline and resuspended in 7H9 base media + 0.05% tyloxapol + 0.085% NaCl containing either 5g/L or 50g/L of fatty acid free BSA (fraction V Roche) with no glycerol nor dextrose added ...