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Citations for Roche :
2551 - 2600 of 9048 citations for Cow WAP kazal immunoglobulin kunitz and NTR domain containing protein 2 WFIKKN2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Cell pellets were then collected in RIPA buffer containing Complete Mini protease inhibitor cocktail (Roche Diagnostic Systems, Laval, Canada) and lysed through five cycles of freeze-thaw ...
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bioRxiv - Biophysics 2022Quote: ... The sinoatrial node explants from old animals were first incubated for 20 min in Low-Calcium Tyrode’s containing twice the concentration of collagenase B used in the young (Cf = 1.08 U/ml, Roche 11088807001), followed by the same 30 min digestion with the three other enzymes at the same concentration used for young tissues ...
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bioRxiv - Cell Biology 2022Quote: ... human tissue was homogenized (Dounce homogenizer) in TBS (20 mM Tris–HCl, 140 mM NaCl, pH 7.5) containing protease and phosphatase inhibitors (Roche). Homogenates were ultracentrifuged (4 °C for 60 min ...
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bioRxiv - Biophysics 2022Quote: ... The cell pellets were resuspended in lysis buffer containing 20 mM Tris (pH 8.0) and 150 mM NaCl and supplemented with EDTA-free protease inhibitor cocktail (Roche). The Sf-9 cells were lysed using 50 homogenizing cycles on ice ...
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bioRxiv - Cell Biology 2022Quote: ... 1% lauryl maltose neopentyl glycol (LMNG)) containing a mix of proteases inhibitors (cOmplete Tablets, Roche, 04 693 124 001). After centrifugation at 5,000 g for 10 min ...
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bioRxiv - Immunology 2020Quote: Lungs were aseptically isolated from mice and incubated at 37°C in 10% FBS RPMI media containing 1.4 mg/ml collagenase A (Roche) and 30 μg/ml DNase I for 60 min ...
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bioRxiv - Microbiology 2021Quote: ... which was freed from muscle tissue with a scalpel and floated on PBS containing 2.86 mg/ml dispase II (Roche) for 60min with the epithelial side facing upwards to separate the epithelial tissue from the lamina propria ...
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bioRxiv - Immunology 2020Quote: ... Small intestines were minced into small pieces and digested in RPMI medium containing 0.1 mg/ml DNase I (Roche) and 100 mg/ml Collagenase VIII (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... washed once with 50 mM Tris-HCl (pH 7.5) containing 25% (wt/vol) sucrose and Complete EDTA-free protease inhibitor cocktail (Roche), and then incubated for 10 minutes at RT in the same buffer containing 100 μg/ml lysozyme (Thermo Scientific ...
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bioRxiv - Immunology 2019Quote: ... 4μg of Env DNA containing CMV promoter was combined with 4μg of SG3Δenv backbone and FuGene 6 transfection reagent (Roche Diagnostics) was added as per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Pellets were then incubated in 300 ul 10mM Tris-HCl (pH 7.5) solution containing 100ug/mL proteinase K (Roche) at 60C overnight followed by 30 min at 90C to denature Proteinase K ...
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bioRxiv - Neuroscience 2020Quote: ... pH 7.5, 1 mM EGTA, 10% sucrose, 0.8 M NaCl containing sarkosyl (final 1%, w/v) and protease inhibitor (Roche). After ultracentrifugation at 135,000 × g for 20 min at 25 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Neurons were harvested on DIV14 and lysed by addition of lysis buffer (50 mM Tris pH 8.0 and 1% SDS containing cOmplete Mini Protease Inhibitor (Roche)) and boiling at 95°C for 5 min ...
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bioRxiv - Immunology 2022Quote: ... The remaining tissue was then incubated with constant shaking at 37C in RPMI media containing 0.1mg/ml collagenase/dispase (Roche) and 20µg/ml DNase (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... resuspended in 20mM Tris-HCl pH 8.0 (30 ml per liter of culture) containing complete EDTA-free protease inhibitor cocktail (Roche) and lysed using a Microfluidizer M-110L (Microfluidics ...
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bioRxiv - Immunology 2022Quote: ... Collected tissue was mechanically dissociated by chopping followed by enzymatic digestion for 45 minutes at 37°C in DMEM containing collagenase D (1.5 mg/ml, Roche) and DNase I (60 U/ml ...
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bioRxiv - Immunology 2019Quote: ... 1μg of linearised DNA was incubated in a final volume of 20μl containing transcription reagents and transcription reaction was performed according to standard protocols (Roche).
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... a 183-nucleotide long single-stranded DNA probe containing digoxygenin dNTP was prepared via asymmetric PCR (Roche Molecular Biochemicals) and the following primers ...
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bioRxiv - Cell Biology 2019Quote: Cells were lysed in Triton X-100 lysis buffer (1% Triton X-100 in TBS, pH 7.5) containing 10 μM Leupeptin (Roche) and 1 mM Pefabloc SC (Roche) ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were lysed with TNE (Tris) buffer containing protease (Complete Mini) and phosphatase inhibitors (PhosStop Roche, 50mM Sodium Fluoride). Protein concentrations were determined by Bradford assay and lysates stored at −80°C until use.
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bioRxiv - Immunology 2020Quote: Mice were euthanized and lungs were gently homogenized in HEPES buffer containing Liberase Blendzyme 3 (70 μg/ml; Roche) and DNaseI (30 μg/ml ...
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bioRxiv - Physiology 2021Quote: ... we collected feces of 90 adults that had fed on Arabidopsis wild type leaves for one day in a total volume of 1 mL 20 mM MES buffer pH 6.5 containing protease inhibitors (cOmplete, EDTA-free, Roche). After homogenization with metal beads for 3 min at 25 Hz in a TissueLyzer II and centrifugation at 4 °C for 10 min at 16,000 × g ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Bronchoalveolar lavage (BAL) was collected by instilling the lungs with phosphate-buffered saline containing cOmplete protease inhibitor cocktail (Roche) (0.5 mL x 1 ...
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bioRxiv - Neuroscience 2020Quote: ... The brains were transferred to a 1.5 ml Eppendorf tube containing 500 microliters 1 mg/ml Liberase DH (Roche, prepared according to the manufacturer’s recommendation ...
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bioRxiv - Neuroscience 2019Quote: ... one series of frozen brain slices was homogenized in 1 ml PBS containing complete protease inhibitor (Roche Applied Science) and 1 mM AEBSF (Sigma ...
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bioRxiv - Neuroscience 2020Quote: Cultured cells were lysed in RIPA buffer containing protease inhibitor cocktail (1 tablet/15 mL) (Roche Diagnostics, Mannheim, Germany), sonicated and stored at −80°C until analysis ...
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bioRxiv - Developmental Biology 2019Quote: ... and incubated on a rocker for 10 minutes with 0.4 mL RIPA lysis buffer containing protease inhibitors (cOmpleteTM tablets, Roche) and phosphatase inhibitors (PhosSTOPTM tablets ...
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bioRxiv - Genetics 2020Quote: ... These pieces were then added to 3-4 ml of digestion media containing 0.1 mg/ml Liberase (Roche, 501003280), 100 U/ml DNase I (Sigma ...
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bioRxiv - Immunology 2021Quote: ... brain and dura mater were chopped and subject to enzymatic digestion in Hanks’ Balanced Salt Solution (HBSS) containing 2.5 mg/mL collagenase D (Roche) and 0.1 mg/mL DNase I (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... Pelleted cells were re-suspended in lysis buffer (phosphate buffered saline containing 0.5% Triton-X 100 and complete protease inhibitors [Roche]) and DAPI was added (final concentration 5μM ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were then collected and resuspended in lysis buffer (50 mM Tris-HCl pH 7.4) containing a protease inhibitor cocktail (Roche), lysed by sonication and incubated for 10 min at 25°C with DNase I (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were harvested and resuspended in 20 mM TRIS pH 8.0 and 150 mM KCl containing EDTA free Complete Protease Inhibitor (Roche) followed by lysis by two passes through a STANSTED SPCH-10 high pressure homogeniser from Homogenising Systems (UK ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting pellets were re-suspended in Lysis buffer (300mM NaCl, 50mM Tris pH 7.5, 10% Glycerol) containing the protease inhibitor cocktail (Roche), and lysed using sonication ...
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bioRxiv - Neuroscience 2021Quote: ... PFA was removed and cells were washed 3x with PBS then blocked in a buffer containing 0.2% Triton X-100 (Roche) in 50% normal goat serum (NGS ...
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bioRxiv - Neuroscience 2020Quote: ... Tissues were weighed and minced, and then tissue homogenization buffer (PBS containing 0.04% bovine serum albumin, Complete Protease inhibitors (Roche)) was added at 5 volumes of the tissue weight ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 30 animals per group were dissected in CMFB (CMF containing 0.5% BSA) and dissociated using 1:50 Liberase (2.5mg/mL, Roche 5401135001) in CMFB at 30°C with agitation at 300 rpm for 30 minutes on an Eppendorf ThermoMixer ...
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bioRxiv - Cell Biology 2019Quote: ... 0.1% SDS and 50 mM Tris) containing Complete Protease Inhibitor Cocktail Tablets (Cat. No. 04693116001, Roche Diagnostics, South Africa). Proteins of interest for human were detected with antibodies (Santa Cruz Biotechnology ...
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bioRxiv - Developmental Biology 2021Quote: ... for 2hr at room temperature and then incubated in blocking solution containing anti-Digoxigenin-AP antibody (1:2000; Roche) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Culture supernatant containing retroviruses was harvested after 48 hours and premixed with DOTAP liposomal transfection reagent (Roche, Basel, Switzerland) prior to spin transfection at 2400 rpm at 32°C for 2 hours into target cells ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed twice in PBS at RT and lysis buffer (PBS, containing 0.1% Triton-X100 and 1x Protease Inhibitor (cOmplete EDTA free, Roche) was added (300µL for 6-well plates ...
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bioRxiv - Immunology 2021Quote: Freshly collected feces were resuspended at 0.1mg/mL in 1X PBS containing EDTA-free Complete Mini Protease Inhibitor Cocktail Tabs (Roche) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... They were lysed in polysome lysis buffer containing 100 μg/mL cycloheximide and protease cocktail inhibitor EDTA-free (Roche)) ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was discarded and the pellets were resuspended in ice-cold water containing cOmplete™ protease inhibitors (Roche). Each sample was subsequently passed 10 times through a 26-gauge needle to shear the host macrophage-like cells while leaving the fungi intact ...
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bioRxiv - Neuroscience 2020Quote: The frozen half-brains were weighed and homogenized in 1 ml PBS containing complete protease inhibitor (Roche Applied Science) and 1 mM AEBSF (Sigma ...
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bioRxiv - Immunology 2019Quote: ... De-epithelialized LP was minced and digested in complete RPMI medium (supplemented with 1% Pen/Strep) containing 0.25 mg/mL Liberase TL and 20 U/mL DNase I (both from Roche), stirring at 37°C for 1h ...
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bioRxiv - Immunology 2020Quote: ... The organs were chopped mechanically with scalpels and transferred into gentleMACS C tubes containing DMEM supplemented with 1μg/mL Collagenase D (Roche) and 25mg/mL DNase I (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2021Quote: ... containing 1% NP-40 and 0.25% sodium deoxycholate (modified RIPA) plus a Complete Protease Inhibitor Cocktail (Roche Applied Science) on ice ...
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bioRxiv - Immunology 2021Quote: ... the tissues were finely chopped and transferred into 5ml polystyrene tubes () containing digestion buffer (5ml/sample) made with RPMI medium + Liberase TL (100μg/ml; Roche) + DNaseI (100μg/ml ...
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bioRxiv - Genomics 2021Quote: ... containing 25 Abs260 ribosome units (1 A260 = 12 μg/μL) [5] were digested with 60 U of MNase (Roche) and 60 U of SUPERase·In (Ambion ...
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bioRxiv - Genomics 2021Quote: ... followed by lysing the cells on ice for 3 min in 50 μl of ATAC-seq RSB containing 0.1% NP40 (Roche), 0.1% Tween-20 (Roche) ...