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Citations for Roche :
2801 - 2850 of 9048 citations for Cow WAP kazal immunoglobulin kunitz and NTR domain containing protein 2 WFIKKN2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: Cells were lysed with ice-cold RIPA lysis buffer containing EDTA-free protease inhibitor cocktail and PhosSTOP phosphatase inhibitor (Roche), with DNA-shearing by 21-gauge needle ...
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bioRxiv - Genomics 2020Quote: ... cell pellets of approximately 1-5 million cells digested overnight at 50C in 500 μl lysis buffer containing 100 μg/ml proteinase K (Roche), 10 mM Tris pH 8.0 ...
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bioRxiv - Neuroscience 2022Quote: Right hemispheres of 3 PS19 mice brains of identical ages ranging from 1 to 6 weeks and 1 year old were gently homogenized in 1:10 ratio w/v of TBS buffer containing protease inhibitor cocktails (Roche) using a dounce homogenizer ...
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bioRxiv - Neuroscience 2022Quote: ... and age-matched controls lacking evident tau pathology were gently homogenized at 4°C in 10 mL of TBS buffer containing protease inhibitor cocktails (Roche) using a dounce homogenizer ...
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bioRxiv - Molecular Biology 2022Quote: In vitro transcribed RNA was prepared from linearized plasmid containing the human PTH 3’-UTR (68) using a Biotin RNA Labeling Mix (Roche) and T7 RNA polymerase ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1% Triton X-100, pH 7.4, containing phosphatase inhibitors (10 mM NaF, 1 mM Na3VO4) and protease inhibitors (cOmplete®, Roche) and the protein concentration was adjusted to 2 mg/ml using the Bradford method (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... and were homogenised in TBS pH 7.4, 1% NP-40 containing phosphatase inhibitors (10mM NaF, 1mM Na3VO4) and protease inhibitors (cOmplete®, Roche). Protein concentration was adjusted to 10 mg/ml by the Bradford method (Pierce ...
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bioRxiv - Immunology 2022Quote: ... In short lungs were minced with scissors and incubated in a digestion cocktail containing 1 mg/mL collagenase A (Roche) and 30 μg/mL DNase I (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... dried and solubilized in 0.2 ml Hepes (100 mM) buffer containing cOmplete™ mini EDTA-free protease inhibitor cocktail (Roche) (1 tablet/20 ml of buffer solution) ...
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bioRxiv - Neuroscience 2019Quote: ... Hybridization with Stellaris FISH probes was done in a total volume of 50 μl hybridization buffer containing 50 μg competitor tRNA from E.coli (10109541001, Roche Diagnostics), 10 % Dextran Sulfate (9011-18-1 ...
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bioRxiv - Microbiology 2019Quote: ... in a final volume of 25 μl containing 12.5 ng of genomic DNA and 2X KAPA HiFi HotStart ReadyMix (Kapa Biosystems). PCR reactions were performed in a Thermal Cycle T gradient (Biometra GmbH ...
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bioRxiv - Bioengineering 2020Quote: ... placing the mucus in a beaker of ice-cold PBS containing a cOmplete protease inhibitor cocktail tablet (Roche, Basel, Switzerland). The crude PIM was centrifuged at 11000 × g at 4 °C for 10 min ...
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bioRxiv - Neuroscience 2019Quote: ... the cortical and hippocampal tissues were dissected from mouse brains and homogenized in ice-cold RIPA buffer containing protease and phosphatase inhibitor cocktails (Roche), followed by centrifugation at 20,817 × g for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 200mg of cell pellet was re-suspended in 1/3 w/v of buffer TK buffer (20mM Tris-HCl, 100mM KCl, 10mM MgCl2, 1mM DTT) containing a cocktail of EDTA-free protease inhibitor (cOmpleteTM, Roche) or TK-EDTA buffer (TK buffer supplemented with 20mM EDTA) ...
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bioRxiv - Plant Biology 2020Quote: ... The second index was added to the amplified libraries through a second PCR containing 12.5 μl KAPA HiFi Hotstart ReadyMix (Kapa biosystems), 1.25 μl 5 μM p7 primer (Supplemental Table 3) ...
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bioRxiv - Microbiology 2021Quote: ... each tube contained about 106 of cells in 200 μl of the digestion buffer containing 50 μU of neuraminidase (Roche). The tubes were incubated at 37°C for one hour ...
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bioRxiv - Neuroscience 2020Quote: Equal amounts of protein were collected in co-immunoprecipitation buffer (10 mM HEPES, 140 mM NaCl, 0.5% NP-40, pH = 7.4) containing protease inhibitors (Complete mini, Roche Applied Science) and phosphatase inhibitor (100 mM sodium orthovanadate ...
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bioRxiv - Biochemistry 2020Quote: ... The thorax and the abdomen were then washed with 3 ml of pre-chilled 0.1 M KBP pH 7.4 homogenization buffer (HB) containing 1x protease inhibitor (Roche Complete ULTR). The complex was homogenized in 20 ml of HB using 40 ml glass Dounce homogenizer with a loose B pestle (Wheaton Science ...
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bioRxiv - Neuroscience 2021Quote: ... The blocking solution was removed and replaced with antibody solution containing 1% heat inactivated goat serum and a 1:5000 dilution of anti-DIG-AP antibody (Roche). Slides were then incubated overnight at 4°C in a humidified chamber ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were lysed using RIPA buffer (50 mMTris–HCl, 10 mM MgCl2, 20% Glycerol and 1% Triton X-100) containing protease and phosphatase inhibitors (Roche). Protein concentration was determined by BCA assay (Pierce ...
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bioRxiv - Neuroscience 2020Quote: Cells of 5 pooled organoids were washed with PBS and then lysed in RIPA buffer containing complete protease and phosphatase inhibitor cocktail (Roche). Lysates were cleared by centrifugation at 20.000g for 10min and quantified with the Pierce™ BCA Protein Assay kit (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: Total cellular protein was isolated from exponentially growing cells and lysed in ice cold RIPA buffer containing 1x cOmplete Mini Protease Inhibitor Tablets (Roche). Protein concentration were quantified and 75 ug resolved (SDS-PAGE ...
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bioRxiv - Neuroscience 2020Quote: ... frozen tissue was mechanically homogenized in TES buffer (4% Tris Base pH 7.5, 0.1 mM EDTA, 100 mM Na2Cl) containing protease inhibitor (PI) (cOmplete, EDTA-free Protease Inhibitor Cocktail (Roche, Mannheim, Germany)) with the VDI 12 homogenisator (VWR ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were resuspended in ice-cold NP-40 Buffer (150mM NaCl, 1% NP-40, 50mM Tris pH8) containing EDTA free protease inhibitors (complete cocktail, Roche) and lysed using a 1ml tissue homogenizer (Fisher) ...
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bioRxiv - Plant Biology 2020Quote: ... with added Roche cOmplete protease inhibitor (# 11836170001, Roche. Samples were further diluted 21 times in 5x Laemmli loading buffer containing Roche protease inhibitor ...
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bioRxiv - Cell Biology 2021Quote: ... Approximately eight million cells were harvested for each sample and lysed into Pierce lysis buffer (Thermo) containing Complete protease inhibitors (Roche). Lysate was clarified by centrifugation at 10,000 g for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... At 80% confluency the cells were harvested on ice by scraping and washed once with PBS containing protease inhibitors (Roche). The cells were pelleted at 300g for 5 min at 4°C and were washed once with KPBS buffer (136 mM KCL ...
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bioRxiv - Plant Biology 2021Quote: ... qPCR reactions were performed in a 10-μl volume containing 5 μl of LightCycler 480 SYBR Green I Master (Roche), 4 μl of the corresponding primer pair (1.5 μM each) ...
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bioRxiv - Genetics 2020Quote: ... These pieces were then added to the 3-4 ml of digestion media containing 0.1 mg/ml liberase (Roche, 501003280), 100 U/ml DNase I (Sigma ...
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bioRxiv - Immunology 2021Quote: ... Lungs were cut into small pieces and digested in 5 mL of RPMI containing DNase I (100 μg/mL, Roche), Dispase II (800 μg /mL ...
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bioRxiv - Neuroscience 2019Quote: The brain samples were homogenised in 5 volumes (w/v) of sterile PBS containing cOmplete Mini EDTA-free Protease Inhibitor Cocktail tablets (Roche), sodium fluoride (10 mM ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were briefly washed in ice-cold PBS and lysed in RIPA buffer containing phosphatase inhibitor cocktail (PhosStop, Roche, USA) and protease inhibitor cocktail (HaltProtease Inhibitor Cocktail ...
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bioRxiv - Cancer Biology 2021Quote: 160 μl DMEM containing 10 % FBS were added to the bottom wells of xCELLigence Real-Time Cell Analyzer CIM plate (Roche), as chemo-attractant ...
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bioRxiv - Cell Biology 2021Quote: ... Cells lysed with lysis buffer were incubated in the buffer containing protease and phosphatase inhibitor cocktails (04693159001 and 04906845001, Roche), 1 mM serine protease inhibitor PMSF (phenylmethylsulfonyl fluoride ...
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bioRxiv - Cell Biology 2021Quote: The cell pellet was resuspended in 1 mL of ice-cold PBS containing 0.1% NP-40 supplemented with proteinase inhibitor cocktail (PIC) (cOmplete™, Mini, EDTA-free, Roche). The cell suspension was vortexed for five seconds at maximum speed ...
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bioRxiv - Cell Biology 2022Quote: ... Triton X-100 (1%vol/vol)) containing PMSF (final concentration 1 mM) and complete protease inhibitor cocktail (without EDTA, from Roche). A 500 μl volume of glass beads was added to the tubes ...
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bioRxiv - Molecular Biology 2022Quote: ... was run on a 15% polyacrylamide gel containing 7M urea and 10 µg/ml APM and then transferred to positively charged nylon membranes (Roche). Pre-hybridization and hybridization steps were performed with Dig Easy Hyb (Roche) ...
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bioRxiv - Immunology 2022Quote: One million cells were collected by centrifugation and resuspended in cold lysis buffer (1% Triton X-100 in PBS) containing protease inhibitors (Complete protease inhibitor cocktail, Roche) and was incubated on ice for 30 minutes ...
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bioRxiv - Physiology 2022Quote: Mouse platelets were isolated using modified standard procedures,[50] as previously outlined.[44] Washed platelets were resuspended directly in NP-40 lysis buffer containing Complete protease inhibitors (Roche). Glomeruli were isolated using magnetic bead perfusion as previously described,[48 ...
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bioRxiv - Neuroscience 2022Quote: Flash frozen striatal samples were homogenized in a RIPA buffer (Pierce; 89900) containing freshly added phosphatase (Pierce) and protease (Roche) inhibitors ...
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bioRxiv - Cell Biology 2022Quote: ... collected and lysed with NP-40 extract buffer (50 mM Tris, pH 8, 150 mM NaCl, 1 % NP-40) containing protease inhibitors (#11873580001 Roche) and 30 µg of lysate was migrated in 10 % SDS-PAGE gels (Bio-Rad Laboratories) ...
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bioRxiv - Developmental Biology 2022Quote: ... and prepared for flow cytometry immunostaining as follows: Matrigel-embedded organoids were digested using a HBSS solution containing 10 U/ml dispase II (Roche) and 125 U/ml of collagenase type IV (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... The cortices were immediately removed from the skull and homogenized in RIPA buffer containing protease inhibitors (Roche cOmplete Ref. 11697498001). Homogenates were cleared by centrifugation at 500 x g twice for 7 min ...
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bioRxiv - Microbiology 2022Quote: ... UK) at 1kbar (10g of cells in 40ml final of 20mM Tris pH 8.0 containing protease inhibitor (Roche, EDTA free), at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... islets were pre-incubated for 1 hour in KRBH buffer (described in in vitro calcium imaging) containing 1% w/v BSA (10775835001, Roche) and 3 mM glucose before incubation with 11 mM glucose ± agonists in KRBH in a shaking 37°C water-bath (80 rpm ...
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bioRxiv - Neuroscience 2020Quote: ... Protein aggregation was evaluated by western blot in total cell extracts prepared in 1% Triton X-100 in PBS containing proteases and phosphatases inhibitors (Roche). Protein quantification was performed with the Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Neuroscience 2020Quote: ... cells were washed once with room temperature PBS and then lysed in a solution of 1 volume 5x SDS-PAGE sample buffer (containing fresh 5% (v/v) beta-mercaptoethanol) to 4 volumes of Immunoprecipitation buffer (IPB; (Thomas et al. 2012)) containing fresh Protease Inhibitor Cocktail (Roche) and 1 μM microcystin-LR ...
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bioRxiv - Immunology 2020Quote: ... Labelled cells were washed twice with 1mL FACS buffer and resuspended in 500uL FACS buffer containing DNAse I (10ug/mL, Roche). Sorting of the cells was performed into 500uL RNAlater on FACSAria III (BD Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: ... and incubated for 30 min at 37 °C in a solution (1 ml per well) containing 10 U/ml of dispase II (Roche) and 125 U/ml of collagenase type IV (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed with cold phosphate buffered saline (PBS) then scraped into cold PBS containing protease inhibitors (Roche, Laval, QC) and 5 mM sodium fluoride (New England Biolabs ...