Labshake search
Citations for Roche :
2501 - 2550 of 3852 citations for hsa mir 635 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: The qRT-PCR was conducted on a LightCycler® 96 system (Roche, Basel, Switzerland) using a DyNAmo ColorFlash SYBR® Green qPCR Kit (Finnzymes ...
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bioRxiv - Microbiology 2020Quote: ... and subsequently quantified by quantitative PCR against Illumina DNA Standards (Kapa Biosystems, Wilmington, MA). Sequencing was carried out on a MiSeq Desktop Sequencer (Illumina Inc ...
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bioRxiv - Microbiology 2020Quote: ... and SYBR green PCR analysis was performed using Kapa HiFi HotStart Ready Mix (Roche) 16 ...
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bioRxiv - Microbiology 2020Quote: ... PCR reactions were performed using the Kapa Library Amplification Kit (Kapa Biosystems, Massachusetts, USA) and BSA 400ng/μL ...
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bioRxiv - Biochemistry 2020Quote: ... released DNA was purified from protein components (High pure PCR Product Purification Kit, Roche) and chromatin quality analysed using EtBr agarose gel electrophoresis (1.3 % Biozym ME Agarose ...
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bioRxiv - Biochemistry 2020Quote: ... released DNA was purified from protein components (High pure PCR Product Purification Kit, Roche) and analysed using ethidium bromide (EtBr ...
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bioRxiv - Molecular Biology 2019Quote: ... by real-time PCR on a LightCycler® 480 (LC480) (Roche Applied Science, Germany). Real-time PCR assays were performed in a final volume of 12 µL using the LightCycler® 480 Probe Master Mix 1X (Roche Applied Science ...
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bioRxiv - Cell Biology 2019Quote: ... Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed using SYBR Green (Roche) on a LightCycler 480 (Roche) ...
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bioRxiv - Microbiology 2019Quote: ... qRT-PCR reactions were performed in a Light Cycler 480 II (Roche, Basel, Switzerland) using 1 μg of cDNA ...
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bioRxiv - Molecular Biology 2020Quote: ... Q-PCR was performed according to standard procedures using FastStart SYBR Green Master (Roche) and a combination of 18SFw and 18SR primers for 18S rDNA (226 bp long product ...
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bioRxiv - Genetics 2021Quote: ... SYBR Green-based quantitative PCR detection was performed using FastStart Universal SYBR Green (Roche) on a StepOne Plus (Applied Biosystems ...
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bioRxiv - Genetics 2020Quote: ... The real-time PCR reaction was run on LightCycler 480 (Roche Diagnostics, Mannheim, Germany) using TaqMan premade gene expression assays (Applied Biosystems ...
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bioRxiv - Immunology 2020Quote: The resulting cDNA was used as template for High Fidelity PCR amplification (KAPA, Roche) using a set of 6 FR1-specific forward primers 30 including sample-specific barcode sequences (6bp ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR reactions were carried out using LightCycler 480 SYBR green I mastermix (Roche). Results were analysed using the comparative CT (ΔΔCT ...
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bioRxiv - Biochemistry 2021Quote: ... and libraries were quantified by Q-PCR using the Kapa Library Quantification Kit (Roche). RNA-seq experiments were performed on an Illumina HiSeq3000 using a paired-end read length of 2x150 pb ...
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bioRxiv - Developmental Biology 2021Quote: ... qPCR was performed on the LightCycler 2.0 Real-Time PCR system (Roche Life Science). Primer pairs were designed to span an exon-exon junction or at least one intron (Table S3) ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were tested by real time PCR in a cobas z480 analyzer (Roche Diagnostics) using the cobas EGFR Mutation Test ...
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bioRxiv - Developmental Biology 2020Quote: ... Additional rounds of PCR were then performed using KAPA HiFi HotStart Ready mix (Roche), initially with primers containing the adaptors (Universal adaptors ...
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bioRxiv - Genetics 2021Quote: Human BE5.1 was amplified using high fidelity PCR (Expand high fidelity system, Roche, UK) from human DNA (Cambio ...
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bioRxiv - Cancer Biology 2021Quote: ... qRT–PCR was performed using a LightCycler 480 II instrument (Roche Molecular Systems, Inc.) and TB Green Premix Ex Taq II (Tli RNase H Plus ...
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bioRxiv - Immunology 2020Quote: ... qRT-PCR was performed in triplicate using LightCycler® 480 SYBR Green Mix (Roche). The standard double delta Ct method was used to determine the relative quantification of target genes ...
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bioRxiv - Microbiology 2020Quote: ... using the PCR master mix KAPA2G Fast™ (KAPA Biosystems, Wilmington MA, United States). Amplified products were visually verified ...
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bioRxiv - Microbiology 2020Quote: ... Triplicate independent real time PCRs are performed using the Light Cycler System (Roche diagnostics). The change in fluorescence of SYBR Green I in every cycle was monitored by the system software ...
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bioRxiv - Developmental Biology 2020Quote: ... was used to perform qPCR on a Lightcycler 96 Real-Time PCR System (Roche). The relative transcript abundance was normalized to atp5h expression as the housekeeping gene control (Wen et al. ...
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bioRxiv - Immunology 2021Quote: ... and real time PCR was performed using KAPA Sybr Fast master mix (Kapa Biosystems) following manufacturer’s instructions using CFX connect real-time PCR detection system (BioRad ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by a second purification with High Pure PCR Product Purification Kit (Roche, Germany). The TOPO TA cloning kit (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... The transcripts were quantified using Light Cycler 480Real-Time PCR system (Roche, Basel, Switzerland). Primers were designed using Primer Quest (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2020Quote: ... and tibiotarsal joint using Roche High Pure PCR template preparation kit (Roche, Indianapolis, IN) as previously described [62,63,90] ...
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bioRxiv - Immunology 2020Quote: ... The LD-PCR mastermix contained 6.25 μl 2× KAPA HiFi HotStart ReadyMix (Roche Diagnostics), 0.125 μl 20 μM PCR_Satija forward primer(30) ...
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bioRxiv - Microbiology 2021Quote: ... Concentration of the pool was monitored with quantitative PCR (KAPA Library Quantification Kit, Roche). Amplicon libraries were mixed with 5% PhiX and sequenced with four MiSeq reagent kits v2 500 cycles (Illumina) ...
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bioRxiv - Genomics 2021Quote: ... and the Kapa HiFi Uracil+ PCR system (cat#: KK2801, Kapa Biosystems, Boston, MA, USA) with the following cycling parameters ...
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bioRxiv - Genomics 2021Quote: ... DIG-labeled LINE DNA probe was prepared by PCR DIG Probe synthesis kit (Roche). Hybridization and autoradiography were performed according to the DIG Application Manual (Roche).
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bioRxiv - Microbiology 2021Quote: ... The 25 μl PCR reaction was performed using a KAPA HiFi HotStart ReadyMix (Roche) and contained 1 μl of extracted fecal microbial DNA and 1 μM of each primer ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted using a Tissue Lysis Buffer and PCR template preparation kit (Roche).
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bioRxiv - Immunology 2022Quote: ... DNA was subsequently PCR amplified by using KAPA HiFi Hotstart Ready Mix (KAPA Biosystems) and Nextera Index Kit (Illumina ...
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bioRxiv - Genomics 2022Quote: ... fourteen non-coding regions of interest were PCR amplified from human genomic DNA (Roche) using the Phusion High-Fidelity PCR Kit (NEB) ...
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bioRxiv - Cell Biology 2022Quote: ... Whole transcriptome amplification (WTA) was performed with KAPA HiFi PCR Mastermix (Kapa Biosystems KK2602) using approximately 6,000 beads per 50 μL reaction volume ...
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bioRxiv - Cell Biology 2022Quote: ... Quantative PCR was performed using the LightCycler 480 SYRB Green I Master (Roche, 04707516001). The raw data was analyzed by absolute quantification/2nd derivative of three independent biological replicates with each being the average of three technical replicates.
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bioRxiv - Cancer Biology 2022Quote: ... PCR reactions were performed in triplicate using the LightCycler 480 instrument (Roche Life Science). The initial denaturation was performed at 95 °C for 7 minutes (ramp rate 4.8 °C/s) ...
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bioRxiv - Genomics 2022Quote: ... DNA was amplified by PCR using KAPA2G FAST Genotyping Mix (Kapa Biosystems, Wilmington, MA) using primers listed in Table 1 ...
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bioRxiv - Immunology 2022Quote: ... Library molarity was measured via quantitative PCR with the KAPA Library Quantification Kit (Roche) on a BioRad CFX Connect thermal cycler ...
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bioRxiv - Genetics 2022Quote: ... Amplification was performed using the Expand Long Template PCR system (Roche Diagnostic, catalogue #11681834001) with 5% DMSO added ...
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative qRT-PCR was performed with LightCycler® 480 SYBR Green I Master (Roche) using CFX96 Touch Real-Time PCR Detection System (BIORAD ...
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bioRxiv - Microbiology 2022Quote: ... The probes were then purified using a PCR clean up kit (Roche Applied Science). The oligonucleotides had been ordered with 5’ biotinylated ends allowing for subsequent complex detection ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative PCR (qPCR) was performed using LightCycler® 480 II system (Roche Diagnostics, Switzerland) with the following conditions ...
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bioRxiv - Neuroscience 2022Quote: ... Real-time quantitative PCR was performed on a LightCycler 1.5 (Roche diagnostics Ltd, UK) using the QuantiTech SYBR Green PCR kit (Qiagen Ltd) ...
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative PCRs were performed using LightCycler® SYBR Green I Master (Roche, Cat# 04887352001) with primers listed in Table S3 on a LightCycler® LC480 instrument (Roche) ...
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bioRxiv - Molecular Biology 2022Quote: ... and β-actin were analyzed by real-time PCR on a LightCycler (Roche Diagnostics) using the LightCycler FastStart DNA Master SYBR Green I kit (Roche Diagnostics ...
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bioRxiv - Neuroscience 2022Quote: ... qRT-PCR was performed using the LightCycler480 SYBRGreen I Master1 kit (Roche Life Science) and the CFX Connect Real-Time PCR Detection System (BioRad) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The 10 μl PCR mixture consisted of 5 μl KAPA2G Robust HotStart ReadyMix (Roche), 1 μl molecular grade water ...