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Citations for Roche :
2401 - 2450 of 3852 citations for hsa mir 635 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was amplified using KAPA HiFi HotStart PCR kit (Kapa Biosystems, KK2501) according to the manufacturer’s guidelines and specific primers flanking the CRISPR cut site (with the CRISPR cut site off center ...
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bioRxiv - Genomics 2023Quote: DNA amplicons for NGS were generated by PCR using KAPA HiFi HotStart (Roche), according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... with 6-8 cycles of PCR amplification using KAPA-HiFi (Kapa Biosystems, KK2602). DNA library was sequenced using an Illumina NextSeq 500 75-cycle high output kit.
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bioRxiv - Pathology 2023Quote: ... Splicing analysis was carried out by PCR amplification with FastStart Taq Polymerase (Roche) using specific primers (Supplementary Table 3) ...
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bioRxiv - Immunology 2023Quote: ... followed by 25 cycles of PCR using KAPA HiFi master mix (KAPA Biosystems) using G115 WT TCR in pMIG as template ...
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bioRxiv - Biochemistry 2023Quote: ... in a real-time PCR device (Light Cycler 480, Roche Diagnostics, Mannheim, Germany). Fluorescence was recorded at 465 nm excitation and 580 nm emission wavelengths ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR reactions were performed in a LightCycler96® instrument (Roche, Basel, Switzerland) real-time system under the following conditions ...
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bioRxiv - Plant Biology 2023Quote: ... and used as templates for qRT-PCR analysis using LightCycler 480 (Roche, Switzerland). Primers used for gene expression are listed in Table S1.
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bioRxiv - Cancer Biology 2023Quote: ... DNA was purified using a High Pure PCR Template Preparation Kit (Roche, 11796828001) and q-PCR was performed as describe above ...
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bioRxiv - Cancer Biology 2023Quote: ... Cytosolic ribosome fractions were collected and digested with PCR grade proteinase K (Roche) in the presence of 1% SDS prior to RNA isolation using TRIzol (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... qPCR was performed on a LightCycler® 480 Real-Time PCR System (Roche) with AceQ qPCR SYBR Green Master Mix (Q111-02 ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified using the High Pure Purification Kit (Roche Applied Science). The oligonucleotides used to amplify and sequence the different hit-related genes are listed in Table S4.
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bioRxiv - Neuroscience 2023Quote: ... with the real-time PCR system (Light Cycler 480, Roche; or CFX384, BioRad). The number of such transgenes was quantified by absolute quantification methods against standard plasmids of pre-quantified concentration ...
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bioRxiv - Cell Biology 2024Quote: ... 12.5 mM EDTA) containing 100 μl proteinase K (recombinant PCR grade solution, Roche) was added to each sample and digested at 65 °C for 15 min ...
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bioRxiv - Developmental Biology 2024Quote: ... Linearized plasmid DNA and PCR products were used to synthesize digoxigenin-UTP (Roche) labeled antisense probes with RNA polymerases from Promega and RNA probes were purified with Illustra ProbeQuant G-50 microcolumns (GE Healthcare ...
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bioRxiv - Genomics 2024Quote: ... quantitative (q)PCR analysis was then performed using SYBR Green mix (Kapa Biosystems), and the relative expression was determined by the 2-ΔΔCt method on a QuantStudio 6 qPCR machine (Thermo Fisher) ...
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bioRxiv - Neuroscience 2024Quote: ... qRT-PCR was performed using a LightCycler 480 real time machine (Roche, Switzerland). For RT-PCR ...
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bioRxiv - Cancer Biology 2024Quote: ... and expanded by PCR using Roche KAPA HiFi HotStart ReadyMix (Roche cat. KK2602). hSTARR-ORI vector (Addgene #99296 ...
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bioRxiv - Molecular Biology 2024Quote: ... PCRs were performed on a Roche LC80 using LightCycler 480 Probes Master (Roche).
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bioRxiv - Microbiology 2024Quote: ... and the PCR was run on the LightCycler 480 System (Roche, Basel, Switzerland) using the KAPA SYBR Fast qPCR Kit (Nippon Genetics ...
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bioRxiv - Genomics 2024Quote: ... The post-enrichment PCR amplification was performed using KAPA HiFi HotStart ReadyMix (Roche) and IS5 and IS6 primers as described above ...
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bioRxiv - Biochemistry 2024Quote: ... The eluted DNA was PCR amplified with KAPA HiFi 2x HotStart ReadyMix (Roche) and 10x Library Amplification Ready Mix primers (Roche ...
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bioRxiv - Plant Biology 2024Quote: ... PCRs were performed using a KAPA SYBR FAST qPCR Kit (KAPA Biosystems, USA) with the Thermal Cycler Dice Real Time System (Takara) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The resulting cDNAs were analyzed by real-time PCR (LightCycler 480 II, Roche), and the cycle threshold was determined from the amplification curve as an index for relative mRNA content in each reaction.
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bioRxiv - Synthetic Biology 2024Quote: PCR preparation of samples for sequencing was conducted using KAPA HiFi (Roche KK2101) for a total of 24 cycles in a two-step PCR ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative PCR was performed using the Roche Light Cycler 480II Assay system (Roche). The levels of different mRNA species were calculated with the delta-delta CT method and normalized to Actb ...
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Characterization of epigenetic alterations in esophageal cancer by whole-genome bisulfite sequencingbioRxiv - Cancer Biology 2021Quote: ... DNA fragments were then PCR amplified by HiFi HotStart Uracil and ReadyMix (Kapa Biosystems). The clustering of the index-coded DNA samples was performed by Illumina cBot Cluster Generation System ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were double size-selected using the KAPA Pure beads (Roche, Cat#: 07983280001) to remove fragments larger than 500 bp (0.65X beads:sample ratio ...
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bioRxiv - Cell Biology 2020Quote: ... Expression levels of individual transcripts were determined by quantitative PCR using SYBR Green (Roche) for detection ...
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bioRxiv - Cell Biology 2020Quote: ... Real-time PCR was performed using KAPA SYBR FAST qPCR master mix (Kapa Biosystems) on an Eppendorf Realex Master cycler ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: ... Custom DIG-labeled probes were generated using PCR DIG Probe Synthesis Kit (Roche #11636090910) and blots were hybridized for 16 hours at 48°C with a final concentration of 25ng/ml probe in 10ml DIG EasyHyb buffer rolling in hybridization tubes ...
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bioRxiv - Microbiology 2019Quote: ... and latterly also into cobas PCR Media tubes (Roche Molecular Systems, Pleasanton, CA, USA), for stabilization during transportation to Oxford ...
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bioRxiv - Microbiology 2019Quote: ... and cDNA analysed on custom real-time ready 384 well custom PCR arrays (Roche). Arrays contained the following murine targets ...
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bioRxiv - Molecular Biology 2021Quote: ... The PCR product was purified with 1.5x ratio KAPA pure bead (Roche Cat# KK8002) and then eluted into water ...
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bioRxiv - Evolutionary Biology 2021Quote: ... qPCR was performed on a LightCycler 480 real-time PCR cycler (Roche, Basel, CH) using Luna Universal qPCR master mix (NEB ...
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bioRxiv - Developmental Biology 2020Quote: ... Each PCR product was ligated into Eam1105I-digested pJC53.2 vector (Quick Ligation Kit, Roche) for use in ISH and RNAi experiments (Collins et al. ...
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bioRxiv - Microbiology 2022Quote: ... 0.5 µM final) for PCR amplification by using KAPA HiFi HotStart ReadyMix (KAPA Biosystems). The PCR program included 95°C for 3 min ...
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bioRxiv - Molecular Biology 2020Quote: ... and libraries were quantified by Q-PCR using the Kapa Library Quantification Kit (Roche). RNA-seq experiments were performed on an Illumina HiSeq3000 using a paired-end read length of 2×150 pb.
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bioRxiv - Molecular Biology 2021Quote: ... QHR-4C libraries were purified by the High-Pure PCR Product Purification kit (Roche).
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bioRxiv - Molecular Biology 2021Quote: PCR reactions were performed in triplicate in 384 well plates with SYBR Green (Roche) and specific primers ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time PCR was conducted using LightCycler® 480 SYBR Green I Master (Roche) on a LightCycler® 480 System (Roche) ...
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bioRxiv - Genomics 2020Quote: ... Library prep PCR was then done by adding 6.75 μl KAPA HiFi MasterMix (Roche), 0.75 μl DMSO and 0.75 μl EvaGreen (Biotium) ...
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bioRxiv - Genomics 2020Quote: ... The cDNA samples were subjected to PCR using KAPA HiFi DNA Polymerase (Kapa Biosystems) and Ligation Sequencing Kit Primer Mix ...
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bioRxiv - Plant Biology 2019Quote: ... The qRT-PCR reactions were performed on a Light Cycler 96 System (Roche, USA) using the SYBR premix ExTaq™ II (Takara ...
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bioRxiv - Genetics 2019Quote: ... Secondary PCR products were cleaned and size selected using KAPA Pure Beads (Roche #KK8002) according to the manufacturer’s protocol for a 0.55X-0.75X double-sided size selection ...
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bioRxiv - Developmental Biology 2019Quote: ... Quantitative PCR was performed using Light Cycler 480 SYBR Green I Master Mix (Roche) in a LightCycler 480 II Instrument ...
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bioRxiv - Genomics 2019Quote: ... Index tagged samples were amplified (6 cycles of PCR, KAPA HiFi kit, KAPA Biosystems), quantified (Accuclear dsDNA Quantitation Solution ...
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bioRxiv - Evolutionary Biology 2019Quote: ... qPCR assays were performed using the LightCycler® 480 Real-Time PCR system (Roche). All reactions were performed in triplicate for the three biological replicates ...
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bioRxiv - Biophysics 2019Quote: ... The DSF assay was conducted on a LightCycler 480 II Real-Time PCR (Roche) with the excitation and emission wavelengths set to 465 and 580 nm ...
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bioRxiv - Genetics 2019Quote: ... Amplification products were detected on the LightCycler 480 II Real-Time PCR instrument (Roche). LightCycler 480 Software was used to quantify products by absolute quantification analysis using the second derivative maximum method ...