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Citations for Roche :
2401 - 2450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... using a LightCycler 96 System (Roche, Basel, Switzerland). The transcript level of each gene was normalized to that of PP2A (At1G13320) ...
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bioRxiv - Plant Biology 2024Quote: ... 1% Triton X-100 and 0.1% protease inhibitor cocktail (Roche)] ...
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bioRxiv - Plant Biology 2024Quote: ... 1 mM EDTA and 0.1% protease inhibitor cocktail (Roche)] and three times with 1 X PBS ...
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bioRxiv - Bioengineering 2024Quote: ... The media was removed and 150 μL of serum free RPMI containing 0.25 mg/mL Liberase TL (Roche 054010120001) were added to the samples ...
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bioRxiv - Plant Biology 2024Quote: ... and 0.1% protease inhibitor cocktail (Roche)] for 30 minutes on ice ...
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bioRxiv - Plant Biology 2024Quote: ... using 0.7X-1.2X library volume of AMPure Beads following the manufacture’s protocol (Roche Holding AG). Ligated libraries were then amplified using PCR and cleaned up twice with 1.2x library volume of AMPure Beads to generate final purified libraries ...
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bioRxiv - Plant Biology 2024Quote: CUT&RUN and greenCUT&RUN libraries were constructed using the KAPA HyperPrep Kit (Roche Holding AG), with minor modifications ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and quantitative PCR (KAPA Biosystems). The sequencing library was clustered on one lane of a flowcell and loaded on the Illumina HiSeq instrument (4000 or equivalent ...
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bioRxiv - Plant Biology 2024Quote: ... 1% PVP and 0.1% protease inhibitor cocktail (Roche)].
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bioRxiv - Plant Biology 2024Quote: ... 0.1% protease inhibitor cocktail EDTA-free (Roche)] ...
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bioRxiv - Biochemistry 2024Quote: ... and a complete EDTA-free inhibitor cocktail (Roche). Lysis was achieved by two passes through a cell disruptor at 20 and 28 kPsi ...
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bioRxiv - Plant Biology 2024Quote: ... or Fluorescein-12-UTP (Roche, Cat No: 11373242910) was prepared (Additional file1) ...
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bioRxiv - Plant Biology 2024Quote: Samples were then rehydrated and treated with Proteinase K 10µg.ml−1 (Roche, Cat No: 3115836001) for 10 min at room temperature (RT) ...
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bioRxiv - Biochemistry 2024Quote: ... Next, 50 µl of lytic buffer was added (2% NP-40, protease inhibitors (Roche), 0.2 µM LargeBiT (produced in house ...
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bioRxiv - Plant Biology 2024Quote: ... 0.5mg.ml−1 tRNA (ROCHE 10109509001), 0.01% Tween-20 ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were mounted in Citifluor AF1(Agar Scientific, Cat No: AGR1320)/PBS solution (3:1) with (optional a DAPI (Roche, Cat No: 10236276001) to a final concentration of 10µg.ml−1 ...
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bioRxiv - Plant Biology 2024Quote: ... Fab fragments (Roche, Cat No: 11207733910) and visualized with Vector® VIP Substrate Kit ...
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bioRxiv - Plant Biology 2024Quote: ... with DNAse I treatment (Roche). Quantitative PCR was carried out using 5x PyroTaq qPCR mix Plus EvaGreen (CMB Cultek Molecular Bioline ...
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bioRxiv - Plant Biology 2024Quote: ... Fab fragment (Roche 11093274910) and for Fluorescein with Anti-Fluorescein-AP ...
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bioRxiv - Plant Biology 2024Quote: ... was performed in 2-day-old cells according to the manufacturer’s protocol (TMR red in situ cell death detection kit, Roche Diagnostics GmbH, Heidelberg, Germany) with modifications as described earlier (Smetana et al. ...
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bioRxiv - Plant Biology 2024Quote: ... Digoxigenin-11-UTP (Roche, Cat No: 11209256910) or Fluorescein-12-UTP (Roche ...
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bioRxiv - Plant Biology 2024Quote: ... Fab fragments (Roche, Cat No: 11426338910). AP activity was visualized with Vector® Blue Alkaline Phosphatase Substrate (Vector Laboratories ...
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bioRxiv - Biochemistry 2024Quote: ... 4°C) the lysate was transferred to 50 μl HA bead slurry (anti-HA affinity matrix, Roche) or 30 μl c-myc bead slurry (EZview red anti-c-myc affinity gel ...
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bioRxiv - Physiology 2024Quote: ... Real-time PCR was performed by using ChamQ Universal SYBR qPCR Master Mix (Q711-02, Vazyme) on the Roche LightCycler96 (Roche, 05815916001) system ...
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bioRxiv - Physiology 2024Quote: ... and cOmplete EDTA-free protease inhibitor cocktail (Roche, #11836170001), snap frozen in liquid nitrogen and stored at –80°C until use ...
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bioRxiv - Physiology 2024Quote: ... digested for 30 minutes at 37°C with 0.5-1 µg/ml of proteinase K (Roche), quickly rinsed with cold PBS ...
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bioRxiv - Physiology 2024Quote: Digoxigenin (DIG)-labeled cRNA probes were generated by in vitro transcription using DIG RNA Labeling Mix (Roche Diagnostics), T7 RNA polymerase (Roche Diagnostics) ...
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bioRxiv - Physiology 2024Quote: ... Fluorescein-labeled cRNA probes for esr1 and esr2a were generated by in vitro transcription using Fluorescein RNA Labeling Mix (Roche Diagnostics), SP6 or T7 RNA polymerase (Roche Diagnostics) ...
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bioRxiv - Physiology 2024Quote: ... RNA probes were synthesised and labelled using the T7-ScribeTM Standard RNA IVT Kit (Madison, Wisconsin, United States) and Digoxigenin-11-UTP (Roche, Basel, Switzerland). Embryos at various stages were fixed in 4% paraformaldehyde overnight at 4°C ...
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bioRxiv - Physiology 2024Quote: ... Hybridization signal was detected with anti-DIG conjugated to alkaline phosphatase (RRID: AB_514497; Roche Diagnostics) and visualized using 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium substrate (Roche Diagnostics) ...
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bioRxiv - Physiology 2024Quote: Coarsely chopped liver fragments from each mouse were digested in 5 ml liberase-based digestion mix (5 ml of DMEM [Thermo Fisher Scientific, Cat #61965059] + 1 mg Liberase™ Research Grade [Roche, Cat #5401127001] + 0.1 μg DNAse I [Sigma-Aldrich ...
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bioRxiv - Physiology 2024Quote: ... ddH2O) supplemented with phosphatase and protease inhibitors (Roche), incubated on a rotating wheel for 2 h at 4°C and sonicated twice for 10 s ...
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bioRxiv - Physiology 2024Quote: ... The reaction volume of 10 μl contained FastStart Essential DNA Green Master Mix (2X, Roche), forward and reverse primers and cDNA template (1:5 diluted) ...
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bioRxiv - Physiology 2024Quote: ... instrument using LightCycler 384-well plates with sealing foil (Roche). The reaction volume of 10 μl contained FastStart Essential DNA Green Master Mix (2X ...
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bioRxiv - Plant Biology 2024Quote: ... and then total proteins were extracted and protein levels were determined by immunoblot analysis with anti-GFP antibody (Roche).
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bioRxiv - Physiology 2024Quote: ... For single cell preparation samples were incubated for 60-min at 37°C with gentle shaking in RPMI media supplemented with 50 mg/ml of Liberase TL (Roche #5401020001), 40 U/ml of DNAse I (Sigma-Aldrich #D4527) ...
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bioRxiv - Biochemistry 2024Quote: ... DSF was performed in a Light Cycler 480 II (Roche Applied Science, Penzberg, Germany) using a 4 °C/min temperature gradient from 20 °C to 95 °C ...
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bioRxiv - Biophysics 2024Quote: ... to which DNAse I was added to a final concentration of 20 µg/ml together with an EDTA-free protease inhibitor tablet (Roche Applied Science). Cells were disrupted by 3 cycles of freeze/thaw and the suspension was then homogenized using an ultrasonic processor (QSonica) ...
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bioRxiv - Biochemistry 2024Quote: ... and 1 × phosphatase inhibitor (PhosSTOP, 04906837001, Roche, Basel, Switzerland).
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bioRxiv - Biochemistry 2024Quote: ... in LightCycler 96 instrument (Roche, Basel, Switzerland). We chose ACTB as the housekeeping gene ...
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bioRxiv - Biochemistry 2024Quote: ... 1% 2-mercaptoethanol and 0.02% bromophenol blue) containing protease inhibitors (0469315900, cOmplete® Mini EDTA-free Protease Inhibitor Cocktail Tablets, Roche, Mannheim, Germany). The samples were separated by SDS-PAGE using 7.5% or 10% acrylamide gels at 100 V for about 2 hours ...
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bioRxiv - Biochemistry 2024Quote: ... nuclease (Pierce) and EDTA-free cOmpleteTM protease inhibitor cocktail (Roche). Cell debris were removed by centrifugation at 1000×g for 20 min ...
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bioRxiv - Biophysics 2024Quote: ... Protease inhibitor (cOmplete; Roche), lysozyme ...
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bioRxiv - Biochemistry 2024Quote: ... and EDTA-free cOmpleteTM protease inhibitor cocktail (Roche). The resuspension was solubilized at 4 °C for 4 h with 2% (w/v ...
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bioRxiv - Cancer Biology 2024Quote: ... phosphatase inhibitor cocktails (Roche #04906837007) were added to the lysis buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with complete™ Mini Protease Inhibitor Cocktail (Roche #11836153001), and subjected to sodium dodecyl sulfate (SDS)- polyacrylamide gel electrophoresis (PAGE) ...
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bioRxiv - Bioengineering 2024Quote: ... and was gap repaired with HiFi HotStart Uracil+ Ready Mix (Kapa Biosystems, Wilmington, MA). USER enzyme (NEB ...
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bioRxiv - Bioengineering 2024Quote: Trastuzumab was purchased from Roche. Trastuzumab Fab was prepared using Pierce Fab Preparation Kit (Thermo Fisher Scientific) ...
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Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... 500 μg/mL of pronase (10165921001, Roche), 150 μg/mL of trypsin (J63688.03 ...
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bioRxiv - Biochemistry 2024Quote: ... Supernatants after high-speed centrifugation were loaded onto His-Tag Purific Resin (Roche), washed and eluted with 50 mM Tris.HCl pH 7.5 ...