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Citations for Roche :
2601 - 2650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... in LightCycler 96 instrument (Roche, Basel, Switzerland). We chose ACTB as the housekeeping gene ...
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bioRxiv - Biochemistry 2024Quote: ... 1% 2-mercaptoethanol and 0.02% bromophenol blue) containing protease inhibitors (0469315900, cOmplete® Mini EDTA-free Protease Inhibitor Cocktail Tablets, Roche, Mannheim, Germany). The samples were separated by SDS-PAGE using 7.5% or 10% acrylamide gels at 100 V for about 2 hours ...
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bioRxiv - Biochemistry 2024Quote: ... nuclease (Pierce) and EDTA-free cOmpleteTM protease inhibitor cocktail (Roche). Cell debris were removed by centrifugation at 1000×g for 20 min ...
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bioRxiv - Biophysics 2024Quote: ... Protease inhibitor (cOmplete; Roche), lysozyme ...
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bioRxiv - Biochemistry 2024Quote: ... and EDTA-free cOmpleteTM protease inhibitor cocktail (Roche). The resuspension was solubilized at 4 °C for 4 h with 2% (w/v ...
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Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... 500 μg/mL of pronase (10165921001, Roche), 150 μg/mL of trypsin (J63688.03 ...
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bioRxiv - Genetics 2024Quote: ... sequencing libraries were prepared using the KAPA Hyper Prep kit (KAPA Biosystems, Kusatsu, Japan) with an optimized manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Plasmid was cut and antisense probe was synthesized by in vitro transcription with Dig RNA Labeling Mix (Roche, Cat. No. 11277073910) or Fluorescein RNA labeling mix (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... 2mM EDTA pH 8.0) containing cOmplete™ Protease Inhibitor Cocktail (Roche). Nuclei were lysed using a probe sonicator (Misonix ...
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bioRxiv - Developmental Biology 2024Quote: ... or Fluorescein RNA labeling mix (Roche, Cat. No. 11685619910) together with T7 RNA polymerase (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... SP6 RNA polymerase (Roche, Cat. No. 10810274001) or T3 RNA polymerase (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... together with T7 RNA polymerase (Roche, Cat. No. 10881775001), SP6 RNA polymerase (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... or T3 RNA polymerase (Roche, Cat. No. 11031163001)(foxl2l ...
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bioRxiv - Developmental Biology 2024Quote: ... Blood glucose concentrations were assessed in blood collected from the tail vein with the Accu-Chek Aviva Connect glucose analyzer (Roche/Mannheim).
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bioRxiv - Developmental Biology 2024Quote: ... 5’-AGCGTTCACATCATATGGCA-3’) using Taq DNA polymerase (Roche, Cat. No. 11146165001). Fragments of foxl2l and id1 were cloned into pGEMT-easy plasmid by TA cloning while nanos2 fragment was cloned into pCS2+ plasmid by BamHI and KpnI ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were stained with BM-purple (Roche, Cat. No. 11442074001). Fluorescent in situ hybridization was performed as described (Brend & Holley ...
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bioRxiv - Cancer Biology 2024Quote: ... HA (Roche, 3F10, 1:10,000), Flag (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... or in RIPA buffer supplemented with protease inhibitor cocktail (Roche) and Halt Phosphatase inhibitor cocktail (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2024Quote: ... and qPCR analysis was performed with SYBR Green (Roche, NJ, USA). Primers for each were designed using NCBI primer blast and were ordered from Integrated DNA Technologies (IDT) ...
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bioRxiv - Biophysics 2024Quote: ... and cOmplete protease inhibitors cocktail tablet (Roche).The lysate was cleared by centrifugation at 20 000 x g for 30 minutes and the supernatant was loaded onto a Ni-NTA resin equilibrated in Buffer A ...
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bioRxiv - Bioengineering 2024Quote: ... livers were perfused at low flow for about 10 min with digestion buffer (low-glucose DMEM supplemented with 1 mM Hepes) containing freshly added Liberase (32 μg/ml; Roche). Digestion was stopped using isolation buffer (low-glucose DMEM supplemented with 10% FBS) ...
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bioRxiv - Biochemistry 2024Quote: ... 2 μg ml-1 pepstatin and protease inhibitor cocktail tablets (Roche). The cells were then lysed by sonication and cell debris was removed by centrifugation at 30,000 g for 45 min at 4° C ...
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bioRxiv - Biophysics 2024Quote: ... were resuspended in 20 mM Tris (pH 7.4) and 300 mM NaCl (50 mL volume) containing complete protease inhibitor cocktail (Roche) and lysed by soni- cation ...
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Refining Spatial Proteomics by Mass Spectrometry: An Efficient Workflow Tailored for Archival TissuebioRxiv - Bioengineering 2024Quote: ... Blocking of endogenous peroxidase was achieved with Inhibitor CM (Roche). Afterward ...
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Refining Spatial Proteomics by Mass Spectrometry: An Efficient Workflow Tailored for Archival TissuebioRxiv - Bioengineering 2024Quote: ... the antibody-antigen complex was visualized using Discovery Chromomap DAB-kit (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: All qPCR reactions were carried out using 384-well plates in 3 technical replicates in 10 µl final reaction volume on the LightCycler 480 System II (Roche). Reaction mix consisted of Power Track SYBR Green Master Mix 2X ...
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bioRxiv - Cancer Biology 2024Quote: ... phosphatase inhibitor cocktails (Roche #04906837007) were added to the lysis buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with complete™ Mini Protease Inhibitor Cocktail (Roche #11836153001), and subjected to sodium dodecyl sulfate (SDS)- polyacrylamide gel electrophoresis (PAGE) ...
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bioRxiv - Bioengineering 2024Quote: ... and was gap repaired with HiFi HotStart Uracil+ Ready Mix (Kapa Biosystems, Wilmington, MA). USER enzyme (NEB ...
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bioRxiv - Bioengineering 2024Quote: Trastuzumab was purchased from Roche. Trastuzumab Fab was prepared using Pierce Fab Preparation Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... The quantitative real-time PCR was done using Maxima SYBR Green/ROX qPCR Master Mix (2X) (Fermentas) and LightCycler 480 (Roche) quantitative PCR machine ...
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bioRxiv - Biochemistry 2024Quote: ... 2 μg ml-1 pepstatin and protease inhibitor cocktail tablets (Roche) and lysed with an EmulsiFlex-C3 homogenizer at pressures above 20,000 psi ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was stored as flow through and the beads were washed 6 times with 1 ml RIPA buffer complemented with 0.25× protease inhibitor (cOmplete, Roche). Bound proteins were eluted from agarose beads with 1 bead volume of Laemmli buffer heated at 95°C for 5 min ...
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bioRxiv - Biochemistry 2024Quote: ... 1 volume of dry parasite pellet was resuspended in 10 volumes of ice-cold RIPA buffer complemented with 1X protease inhibitor (cOmplete, Roche). After one hour of incubation on ice with regular mixing ...
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bioRxiv - Cell Biology 2024Quote: ... GFP (1:1,000, Roche, 13921700), HA (1:5,000 ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with cOmplete EDTA-free protease inhibitor tablets (Roche). Lauryl maltose neopentylglycol (L- MNG ...
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bioRxiv - Biochemistry 2024Quote: ... Supernatants after high-speed centrifugation were loaded onto His-Tag Purific Resin (Roche), washed and eluted with 50 mM Tris.HCl pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... DNaseI and cOmplete protease inhibitor cocktail (Roche)) and sonicated ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with protease inhibitor (cOmplete EDTA-free, Roche) and phosphatase inhibitor (PhosSTOP ...
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bioRxiv - Biochemistry 2024Quote: ... and phosphatase inhibitor (PhosSTOP, Roche). Chromatin was degraded using a Bioruptor sonication rod (Diagenode Diagnostics ...
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bioRxiv - Biochemistry 2024Quote: ... Disodium ATP was obtained from Roche and ADP was obtained from Sigma Aldrich.
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bioRxiv - Biochemistry 2024Quote: ... The cells were lysed by sonification and treated with DNase–I (Roche, Basel, Switzerland) for 30 minutes on ice to remove bacterial DNA ...
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bioRxiv - Biophysics 2024Quote: ... Tumor spheroids were digested into single cell suspensions using 0.028 Wünsch units/ml of Liberase TM Research Grade (Roche, Mannheim, Germany) and 20 μg/ml of DNase I in L-15 with 10 % CS for 1 hour at 37 °C and 250 RPM on an orbital shaker ...
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bioRxiv - Cancer Biology 2024Quote: ... and digested into single cells (tissues) using a mix of collagenase/dispase (Roche), accutase (Millipore) ...
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bioRxiv - Biochemistry 2024Quote: ... Complete protease inhibitor cocktail (Roche) and 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... DNA vectors were transfected using FuGENE 6 transfection reagent (Roche Applied Science, Laval, QC) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... yielding the plasmid constructs pXOON-r5-HT3A and pXOON-gp5-HT3A (Roche Diagnostics, Mannheim, Germany). Point mutations were generated by site-directed mutagenesis using the QuickChange mutagenesis kit (Stratagene ...
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bioRxiv - Biochemistry 2024Quote: ... 50 mM NaCl, 0.1 mM EDTA, 10% [v/v] glycerol, 1% [w/v] digitonin, 2 mM PMSF, 1 x Roche EDTA free protease inhibitor cocktail). After removing the debris by a clarifying spin (12,000 x g ...
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bioRxiv - Biochemistry 2024Quote: ... 2 PhosSTOP easy Pack (ROCHE), Protease Inhibitor cocktail (Sigma) ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei were pelleted at 1,400g for 5 min at 4 °C and lysed in 5Lml LB2 (10LmM Tris-Cl pH 8.0, 200LmM NaCl, 1LmM EDTA, 0.5LmM EGTA, Roche protease inhibitors) for 10 min at room temperature with rotation ...