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Citations for Roche :
2501 - 2550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... the KAPA RNA HyperPrep Kit (Cat.# KK8541, Roche Sequencing Solutions) was used to generate libraries ...
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bioRxiv - Cancer Biology 2024Quote: ... Cytotoxicity was evaluated by measuring LDH release according to the manufacturer’s protocol (Roche). S180 cell line was used as the unrelated control ...
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bioRxiv - Cell Biology 2024Quote: ... Nuclei were pelleted at 800g for 10 min at 4°C and washed twice in buffer 1 and once in freezing buffer (1M Tris pH 8, 100% glycerol, 100mM MgAc2, 0.5M EDTA, 1M DTT, 1x PTase inhibitor cocktail Roche, 20U/µl SUPERase In Ambion). Nuclei were resuspended in freezing buffer ...
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bioRxiv - Cell Biology 2024Quote: ... 1M Tris pH 7.5, 1M MgCl2, 100% Triton X-100, 100mM EGTA, 1M DTT, 1x PTase inhibitor cocktail Roche, 20U/µl SUPERase In Ambion, 1M CaCl2), dounced in a 1m dounce homogenizer with a loose pestle 25 times ...
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bioRxiv - Cell Biology 2024Quote: ... Library molarity was measured via quantitative PCR with the KAPA Library Quantification Kit (Roche KK4824) on a BioRad CFX Connect thermal cycler ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with protease inhibitor cocktail (Roche, 11697498001) and 1 mM PMSF (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... Protease Inhibitor from Roche (11697498001). Wildtype human adenovirus species C2 (HAdV-C2WT ...
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bioRxiv - Cell Biology 2024Quote: ... cOmplete™ Proteasehemmer-Cocktail by Roche were used to stop protein degradation ...
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bioRxiv - Cell Biology 2024Quote: Tail snip DNA was extracted using KAPA Express Extract Kit (Roche). Genotyping PCR was performed with HotStarTaq Master Mix (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... cells were detached using cold PBS and lysed in 350 µl lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 1% Triton X-100) containing protease inhibitor (Roche) for at least 20 min on ice ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was blotted with anti-GFP monoclonal primary antibody (mouse monoclonal, Sigma- Roche #11814460001) at 4°C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... containing 5% beta-mercaptoethanol and protease and phosphatase inhibitors (Roche Complete Mini protease inhibitor ...
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bioRxiv - Cell Biology 2024Quote: ... (anti-GFP (Clones 7.1 and 13.1, Roche), anti-actin ...
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bioRxiv - Cell Biology 2024Quote: ... containing 5% beta-mercaptoethanol and protease and phosphatase inhibitors (Roche Complete Mini protease inhibitor, Sigma-Aldrich #11836153001, and Roche Complete Ultra phosphatase inhibitor ...
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bioRxiv - Cell Biology 2024Quote: ... Trypsin was inhibited using 10 µM phenylmethylsulfonyl fluoride (PMSF, Roche Diagnostics) in a total volume of 1ml PBS for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mM DTT with Protease inhibitor cocktail (Roche) and incubated for 10 min on ice ...
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bioRxiv - Cell Biology 2024Quote: ... The quantitative real-time PCR was done using Maxima SYBR Green/ROX qPCR Master Mix (2X) (Fermentas) and LightCycler 480 (Roche) quantitative PCR machine ...
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bioRxiv - Cell Biology 2024Quote: ... just after the resection the tissue was dissociated into small pieces (∼2 mm × 2 mm × 2 mm) and then digested for 30 minutes at 37°C with Collagenase (2 mg/mL, Roche, Basel, Switzerland) and DNase I (400 U/mL ...
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bioRxiv - Cell Biology 2024Quote: ... and DNase I (400 U/mL, Roche). The digested tissue pieces were then filtered through a 70 µm filter ...
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bioRxiv - Cell Biology 2024Quote: ... Faststart Essential DNA Green Master mix (Roche, Basel, Switzerland) was used to perform qPCR on a Lightcycler 96 Real-Time PCR System (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... The aqueous phase from each MaXtract tube (∼400 µl) was then transferred to 1.5 ml tubes containing 2 µl of 2 µg/µl glycogen (Roche, Cat# 10901393001), 1 ml ethanol was added ...
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bioRxiv - Cancer Biology 2024Quote: ... EDTA-free Protease Inhibitor Cocktail (Roche, Cat# 04693159001) at 200 x g for 3 min ...
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bioRxiv - Cell Biology 2024Quote: ... including a protease inhibitor cocktail (Roche, 04906845001, Basel, Switzerland) and a phosphatase inhibitor cocktail (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... was used to perform qPCR on a Lightcycler 96 Real-Time PCR System (Roche, Basel, Switzerland). The relative transcript abundance was normalized to Ef1α expression as the reference gene control ...
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bioRxiv - Cell Biology 2024Quote: ... and a phosphatase inhibitor cocktail (Roche, 11697498001) for 30 min followed by centrifugation and the supernatant was collected as a protein extract ...
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bioRxiv - Cell Biology 2024Quote: ... Genomic DNA was isolated by lysing cells (10 mM Tris-HCl pH 8.0, 1 mM EDTA, 0.67% SDS, and 125 μg/ml proteinase K from Roche), followed by incubation 4 hours at 55°C with weak agitation ...
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bioRxiv - Cell Biology 2024Quote: ... 10ul/ml cOmplete™ Protease Inhibitor Cocktail (Roche, SKU 11836170001), phosphatase inhibitor cocktail mix (10 mM tetrasodium pyrophosphate ...
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bioRxiv - Cell Biology 2024Quote: ... Lentiviral vectors expressing sgRNAs were transfected into HEK293T cells with lentiviral packaging vectors CMV VSV-G and psPAX2 using XtremeGene 9 transfection reagent (Roche). After 24 h ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with protease inhibitors (cOmpleteMini, Roche). Protein concentration was determined using a Pierce BCA protein assay kit (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... 1 tablet EDTA-free protease inhibitor (Roche, #11836170001). After pellets were fully resuspended ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with protease inhibitors (cOmpleteMini, Roche). Lysates were then transferred to 1.5ml Eppendorf tubes and vortexed vigorously for 30s prior to centrifugation at 21,000g for 10 minutes at 2°C ...
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bioRxiv - Cell Biology 2024Quote: ... Real-time PCR reactions were performed on a LightCycler 480 Instrument II (Roche Life Science) using Luna Universal qPCR Master Mix (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... assay was performed following our previous publication(4, 55) and the instruction of In Situ Cell Death Detection Kit TMR red (Roche, 12156792910). Cells were cultured on coverslips for 36 h ...
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bioRxiv - Cell Biology 2024Quote: ... monosodium salt (WST-1 assay) (Roche, Manheim, Germany, Cat. #5015944001) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Phosphatase inhibitor cocktail (Roche 04906837001) and Protease inhibitor cocktail (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... and Protease inhibitor cocktail (Roche, 04693116001). LDS sample buffer (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... cDNAs were amplified using the LightCycler® 480 SYBR Green I Master (Roche). The relative mRNA abundance was calculated using the LightCycler® software and normalized to Cyclophillin and Tata Binding Protein (TBP ...
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bioRxiv - Cell Biology 2024Quote: ... and a LightCycler® R 96 instrument (Roche Diagnostics, Basel, Switzerland) using specific primer sequences (supplementary Table S1) ...
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bioRxiv - Cell Biology 2024Quote: ... D-Mel (d.mel-2) cells were transfected using X-tremeGene HP DNA transfection reagent (#06366236001, Roche). Two days after transfection ...
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bioRxiv - Cell Biology 2024Quote: ... deparaffinized microsections were rehydrated and incubated with hyaluronidase (4mg/mL in PBS, pH 5.5) followed by pronase (1mg/mL in PBS, pH 7.4, both Roche Diagnostics, Mannheim, Germany). After blocking with 5% bovine serum albumin sections were incubated with a mouse anti-human type II collagen antibody (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... the supernatant was cleaned using SPRIselect beads and 30 ng of cDNA is used as input for the PCR amplification (20 cycles) with Kapa HiFi HotStart Ready Mix (Roche Diagnostics) and addition of Illumina-index sequences ...
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bioRxiv - Cell Biology 2024Quote: ... ATP levels were assessed using the ATP Bioluminescence Assay Kit CLS II (Roche) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with complete Protease Inhibitor Cocktail (Roche) and PhosSTOP (Roche) ...
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bioRxiv - Cell Biology 2024Quote: ... bound and unbound fractions were treated with 0.3 mg ml-1 of Proteinase K (Roche) in digestion buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was extracted using the High Pure FFPET DNA Isolation Kit (Roche). Extracted FFPE DNA was repaired using the NEBNext FFPE DNA Repair Mix ...
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bioRxiv - Cell Biology 2024Quote: Cells were harvested and lysed in RIPA buffer (Roche) containing 1x Halt Protease Inhibitor Cocktail (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... and 1x PhosStop (Roche, 4906837001), clarified at 13,000 g 4°C for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... and 1x PhosStop (Roche, 4906837001) for 30 min on ice ...
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bioRxiv - Cell Biology 2024Quote: ... was transfected into U2OS cells using JetPRIME transfection reagent (Roche Applied Science, Mannheim, Germany), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... cells were lysed in cOmplete Protease Inhibitor Cocktail (Roche) containing RIPA buffer.