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Citations for Roche :
1851 - 1900 of 7633 citations for ssc mir 758 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The 8μm thick tissues were subjected to TUNEL staining according to the manufacturer’s instructions for an In-Situ Cell Death Detection Kit (Cat. 11684817910, Roche Diagnostics, Indianapolis, IN, USA). Sections were then visualized with a fluorescence microscope (Nikon ...
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bioRxiv - Microbiology 2022Quote: ... #439259) or HeV (Advanced Cell Diagnostics Inc #410719) and detected using the Discovery mRNA purple HRP detection kit (Roche Tissue Diagnostics #760-255) on the Ventana Discovery ULTRA staining platform (Roche Tissue Diagnostics) ...
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bioRxiv - Genetics 2022Quote: Southern blot analysis was performed to ensure that the foreign gene Bar was present in randomly excised segments of the T2 transformed soybean genome by following the protocol from the DIG High Prime DNA Labeling and Detection Starter Kit I (Roche, Indianapolis, IN, USA). DNA was extracted from the plants with positive PCR results ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections as previously described (46) (Iba-1 staining was conducted on a Ventana Benchmark using OptiView and UltraView detection kits provided by Roche (Roche Molecular Systems, Inc). Sections were deparaffinized in xylene and rehydrated through an ethanol series ending in distilled water ...
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bioRxiv - Immunology 2022Quote: Tumor tissue sections were subjected to the terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick-end labeling (TUNEL) assay using the In Situ Cell Death Detection Kit (Roche Diagnostics, Indianapolis, IN) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... following by the secondary antibody incubation and chromogenic detection with DISCOVERY Purple and Yellow kits (Ventana-Roche Diagnostics, #760-229 and # 760-239). These chromogenic dyes are covalently deposited and have unique spectra (83 ...
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bioRxiv - Plant Biology 2024Quote: ... was performed in 2-day-old cells according to the manufacturer’s protocol (TMR red in situ cell death detection kit, Roche Diagnostics GmbH, Heidelberg, Germany) with modifications as described earlier (Smetana et al. ...
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bioRxiv - Neuroscience 2024Quote: ... brain sections were processed for DNA strand breaks using Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) from Fluorescence In Situ Cell Death Detection kit (Roche Diagnostic, Indianapolis, IN) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... The three PCR reactions per sample were combined and purified using the High Pure PCR Product Purification Kit of ROCHE™ (Roche Diagnostics GmbH, Mannheim, Germany). Sequencing was performed using the MiSeq™ Illumina® (2 x 300 bp ...
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bioRxiv - Plant Biology 2019Quote: Quantitative reverse transcription PCR was performed using KAPA SYBR FAST ABI PRISM kit (Kapa Biosystems) using a QuantStudio™ 12K Flex Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... we used the Roche PCR High Purity Template Preparation Kit (Roche México, Mexico City, Mexico), following manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... ligation products were amplified by PCR using a KAPA Library Amplification Kit (KAPA Biosystems KK2610) with 500 nM of Illumina multiplex primer (a 5′ primer that adds a P5 capture site ...
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bioRxiv - Cell Biology 2019Quote: ... probes were prepared by using specific primers (Table 2b) using DIG labeling PCR kit (ROCHE). DIG labeled PCR products were purified using the nucleospin gel and PCR cleanup kit (Macherey ‒Nagel).
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bioRxiv - Genomics 2020Quote: Genomic DNA was extracted using a High Pure PCR Template Preparation Kit (Roche, Basel, Switzerland). Whole genome sequencing was carried out using both Illumina MiSeq and Oxford Nanopore MinION ...
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bioRxiv - Plant Biology 2021Quote: ... PCR reactions were performed using KAPA SYBR FAST qPCR Master Mix kit by Kapa Biosystems, with the following cycling conditions ...
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bioRxiv - Genetics 2021Quote: ... and amplified by PCR with the KAPA HiFi HotStart Uracil+ ReadyMix kit (Roche, Basel, Switzerland) (Table S2) ...
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bioRxiv - Genetics 2022Quote: ... Fbxw4 and Fgf8 transcribed from linearized gene-specific probes (PCR DIG Probe Synthesis Kit; Roche). Primers for probe generation are listed in Supplementary Table 3 ...
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bioRxiv - Cell Biology 2022Quote: ... Indexed libraries were quantified by qRT-PCR using a commercially available kit (Roche, KAPA Biosystems) and insert size distribution was determined with the Agilent Bioanalyzer ...
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bioRxiv - Cell Biology 2022Quote: ... Indexed libraries were quantified by qRT-PCR using a commercially available kit (Roche, KAPA Biosystems) and insert size distribution was determined with the Agilent Bioanalyzer ...
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bioRxiv - Bioengineering 2022Quote: ... Polymerase chain reaction (PCR) was performed using KAPA2G Fast/HiFi HotStart ReadyMix kit (Kapa Biosystems). Plasmids and DNA were purified using Monarch kits (NEB ...
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bioRxiv - Genomics 2020Quote: ... The ligated fragments were quantified using quantitative PCR and a KAPA Library quantification kit (Roche), and then submitted for sequencing on an Illumina HiSeqX device running a 2 x 150 bp paired-end configuration (Admera Health ...
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bioRxiv - Genomics 2020Quote: ... qRT-PCR was performed using a SYBR green I master kit (Roche Diagnostics, Indianapolis, USA) with the LightCycler System (Roche LightCycler 480 ...
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bioRxiv - Cell Biology 2019Quote: ... genomic DNA was isolated using the High Pure PCR Template Preparation Kit (Roche Life Science). STR DNA genotyping at DSMZ was carried out as described previously using a nonaplex PCR reaction of eight highly polymorphic STR loci plus Amelogenin-based gender determination (Dirks and Drexler 2013) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Paired-end libraries were prepared with the PCR-free KAPA Hyper Prep Kit (KAPA Biosystems). The 150 bp paired-end reads were sequenced with Illumina HiSeq2500 at the Genomics Facility Basel to a mean coverage of 23 (range 18–38 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and the synthesized primers for qRT-PCR and KAPA SYBR Fast qPCR Kit (KAPA BIOSYSTEMS) were used to perform qRT-PCR with StepOnePlus (Applied Biosystems) ...
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... cDNA was amplified for 20 cycles using KAPA HiFi HotStart ReadyMix PCR Kit (Kapa Biosystems). Aliquots of the amplified cDNA were also used for antibody cloning later ...
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bioRxiv - Microbiology 2020Quote: ... Total genomic DNA was extracted using the High Pure PCR Template Preparation Kit (Roche, Germany) according to the manufacturer’s instructions and the 16S rRNA gene of the isolate was amplified by PCR using 27F:AGAGTT TGATCMTGGCTCAG (Wilmotte et al. ...
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bioRxiv - Microbiology 2020Quote: ... A probe for mGFP ORF was generated using the PCR DIG Synthesis Probe Kit (Roche) and primers P35-P36 (Table S1 ...
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bioRxiv - Immunology 2020Quote: ... q-PCR was performed using the Smart SYBRGreen fast Master kit (Roche Diagnostics, Meylan, France) and a CFX Touch RTPCR Detection System (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... PCR was carried out using KAPA SYBR FAST qPCR Kit Master Mix (KAPA BIOSYSTEMS, KK4602) and primer sets (Supplementary Table 1 ...
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bioRxiv - Genomics 2022Quote: ... Library molarity was measured via quantitative PCR with the KAPA Library Quantification Kit (Roche KK4824) on a BioRad CFX Connect thermal cycler ...
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bioRxiv - Cancer Biology 2022Quote: ... and DNA was extracted using the High Pure PCR template preparation kit (Roche, Cat# 11796828001) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... Libraries were purified using the High Pure PCR Production Purification Kit (11732676001, Roche / Sigma-Aldrich). Libraries were validated on an Agilent 2100 and quantified using quantitative PCR (Q-PCR) ...
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bioRxiv - Genetics 2022Quote: ... cinerea genomic DNA using the KAPA HiFi HotStart PCR kit (Kapa Biosystems, Wilmington, MA, USA). The PCR thermocycling profile used was as follows ...
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bioRxiv - Plant Biology 2023Quote: ... Libraries were prepared with the purified PCR product by the Kapa DNA Hyper Kit (Roche) together with TruSeq DNA UD indexes for Illumina (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were constructed using the KAPA Hyper PCR-free Kit (KR0961, KAPA Biosystems, Wilmington, MA) and sequencing was performed using a NovaSeq S4 XP (Illumina ...
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bioRxiv - Biochemistry 2022Quote: DNA was isolated from tail snips with the High Pure PCR Template Preparation Kit (Roche) per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The pool was quantified by quantitative PCR with a Library Quant Kit (Kapa Biosystems, KK4824) and sequenced on an Illumina NovaSeq 6000 with paired-end 150 bp reads.
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bioRxiv - Neuroscience 2023Quote: ... Library molarity was measured via quantitative PCR with the KAPA Library Quantification Kit (Roche KK4824) on a BioRad CFX Connect thermal cycler ...
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bioRxiv - Genomics 2024Quote: ... and libraries were constructed using on-bead PCR amplification using the KAPA HyperPlus kit (Roche). The constructed libraries were then sequenced using the Illumina NovaSeq 6000 platform (paired-end 50 bp reads ...
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bioRxiv - Microbiology 2024Quote: Total DNA isolated using the High Pure PCR Template Preparation Kit (Roche Diagnostics, Indianapolis, IN) according to the manufacturer’s instructions was used as a template for nested PCR amplification (detection limit 10-2 parasites per sample ...
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bioRxiv - Cell Biology 2024Quote: ... Library molarity was measured via quantitative PCR with the KAPA Library Quantification Kit (Roche KK4824) on a BioRad CFX Connect thermal cycler ...
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bioRxiv - Microbiology 2023Quote: ... Library molarity was measured via quantitative PCR with the KAPA Library Quantification Kit (Roche KK4824) on a BioRad CFX Connect thermal cycler ...
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bioRxiv - Microbiology 2023Quote: ... 5 µl RNA eluate was used in a RVFV RT-qPCR using the LightCycler one-tube RNA Amplification Kit HybProbe (Roche, Almere, The Netherlands) in combination with a LightCycler 480 real-time PCR system (Roche ...
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bioRxiv - Pathology 2023Quote: ... The RT-qPCR reactions were performed as recommended by the kit protocol and run on a Roche Light Cycler (Roche, Indianapolis, IN, USA). The limit for cycle of quantification was defined at less than 29 ...
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bioRxiv - Molecular Biology 2024Quote: RNA was extracted from Lipo246 and Lipo815 cell lines and Reverse transcription (RT) reactions were carried out using Transcriptor RT enzyme (Roche Diagnostics, Basel, Switzerland). Nested PCR was performed using a 2-step PCR reaction as previously described(31).
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bioRxiv - Developmental Biology 2019Quote: Non-radioactive RNA in situ hybridization was performed using a digoxigenin (DIG) RNA labeling kit and PCR DIG probe synthesis kit (Roche; http://www.rochediagnostics.us) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The purified indexed PCR library products were quantified using the KAPA Library Quantification Kit from Roche (cat. no. 07960255001, kit code KK4844), normalized to a concentration of 7 nM ...
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bioRxiv - Immunology 2020Quote: ... ATAC-Seq libraries were then purified using a QIAGEN PCR cleanup kit and quantified using KAPA library quantification kit (KAPA Biosystems, Roche) and sequenced on the Nextseq 550 platform.
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bioRxiv - Immunology 2021Quote: ... ATAC-Seq libraries were then purified using a QIAGEN PCR cleanup kit and quantified using KAPA library quantification kit (KAPA Biosystems, Roche) and sequenced on the Nextseq 550 platform.