Labshake search
Citations for Roche :
2001 - 2050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... blots were probed with 1:500 anti-HA (Roche, #11867423001). Anti-mouse IgG-HRP (Abcam ...
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bioRxiv - Immunology 2024Quote: ... Adherent HEK 293T cells were transfected with Xtremegene HP reagent (Roche) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... and 0.8 µg of transfer plasmid using 5.8 X-tremeGENE HP (Roche). Media was replaced after 16 hours and supernatant harvested after a further 24 hours by filtering through a 0.45 cellulose acetate filter ...
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bioRxiv - Genomics 2024Quote: ... and 0.1% Tween-20 (Roche, 11332465001) in 1x divalent cation-free phosphate-buffered saline ...
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bioRxiv - Genomics 2024Quote: ... All hybridization reagents were purchased from Roche as part of the KAPA Hyper Prep kit ...
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bioRxiv - Genomics 2024Quote: ... Amplified libraries were subjected to hybridization with biotinylated oligonucleotide pools that were designed by the HyperDesign Team (Roche) and synthesized by Roche ...
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bioRxiv - Genomics 2024Quote: ... we used bisulphite-converted product as input in PCR reactions and tested an annealing temperature gradient ranging from 55 to 62C for 30 cycles with Kapa Uracil+ HotStart ReadyMix polymerase (Roche, 7959052001). Samples of each PCR product were run on a 2% agarose gel ...
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bioRxiv - Genomics 2024Quote: ... and synthesized by Roche. All hybridization reagents were purchased from Roche as part of the KAPA Hyper Prep kit ...
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bioRxiv - Genomics 2024Quote: ... using the Biotin and Dig Nick Translation Mix (Roche, Mannheim, Germany). Chromosome preparations were made from fibroblast cell culture as described previously61 ...
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bioRxiv - Genomics 2024Quote: ... and 22-bp Sat III were synthesized as probes and labeled with Dig-dUTP or Bio-dUTP (Roche Diagnostics, Basel, Switzerland) using the Biotin and Dig Nick Translation Mix (Roche ...
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bioRxiv - Genomics 2024Quote: ... and HTO were performed in 1X KAPA HiFi PCR reagent (Roche) with 1 µM of forward primer and 1 µM of reverse primer and thermal protocol as specified below ...
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bioRxiv - Genomics 2024Quote: ... Proteinase K (100 mg/ml, Roche) was then added ...
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bioRxiv - Genomics 2024Quote: ... and libraries were constructed using on-bead PCR amplification using the KAPA HyperPlus kit (Roche). The constructed libraries were then sequenced using the Illumina NovaSeq 6000 platform (paired-end 50 bp reads ...
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bioRxiv - Genomics 2024Quote: ... the tissue fragments were transferred into a Douncer homogenizer containing a protease inhibitor cocktail (PIC, Roche, 1 tablet in 50 ml of PBS) solution immersed in ice and homogenized using pestles A and B (from less to more plunger adjustment) ...
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bioRxiv - Genomics 2024Quote: ... for biotin-labeled probes and rhodamine-conjugated anti-digoxigenin (Roche Diagnostics, Basel, Switzerland) for digoxigenin-labeled probes ...
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bioRxiv - Genomics 2024Quote: ... Adapted fragments were amplified by 12 cycles of PCR using the Kapa Hifi Hotstart NGS library Amplification kit (Roche, Basel, Switzerland), followed by 0.8x AMPure XP (Beckman Coulter Genomics ...
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bioRxiv - Genomics 2024Quote: ... as well as a Kapa library quantification kit for Illumina libraries (Roche, Switzerland), were used ...
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bioRxiv - Immunology 2024Quote: ... minced and digested for 30 min in collagenase D (2 mg/mL, Roche) and DNase I (100 μg/mL ...
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bioRxiv - Genetics 2024Quote: ... and lentiviral envelope vector CMV VSV-G using XtremeGene 9 transfection reagent (Roche). Viral particles were collected 48h after transfection and passed through a 0.45 μm filter to eliminate cells and cell debris ...
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bioRxiv - Genetics 2024Quote: ... and viral envelope vector gag-pol using XtremeGene 9 transfection reagent (Roche). Collection of viral particles and transduction was performed as described for lentiviral particles ...
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bioRxiv - Genetics 2024Quote: ... supplemented with a protease inhibitor cocktail (Complete Mini, Roche Diagnostics). Proteins were run on 4-10% Bis-Tris gels (BioRaD ...
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bioRxiv - Genetics 2024Quote: ... These templates were transcribed in vitro using T7 and digoxigenin-RNA labeling mix (Roche). The quality of the synthesized DIG-labeled RNA probes was confirmed by electrophoresis and dot-blot ...
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bioRxiv - Genomics 2024Quote: ... The PCR was done using 2x Kapa HiFi Master mix (Roche, 09420398001) with the barcoded primers described in the Omni-ATAC protocol ...
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bioRxiv - Genomics 2024Quote: ... cDNA was synthesized with 200ng of the stored RNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche, 04897030001) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... and supplemented with pronase (0.3 µg/ml, Roche, #10165921001). Cartilage fragments were digested in this medium for 90 minutes at 37°C with gentle shaking ...
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bioRxiv - Genetics 2024Quote: ... the SYBR Green I Master mix was used on a LightCycler 480 Real Time PCR System (Roche). The primers are listed in Table S2 ...
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bioRxiv - Genetics 2024Quote: ... 0.5% NP-40) supplemented with Complete Protease inhibitor cocktail (Roche, 11697498001), Complete Phosphatase inhibitor cocktail (Roche ...
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bioRxiv - Genomics 2024Quote: ... 1 U/µl Protector RNase inhibitor (Roche, catalog no. 3335402001), 0.2 U/µl SUPERaseIn (Thermo Fisher ...
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bioRxiv - Genomics 2024Quote: ... DNA was extracted using the MagNA Pure DNA isolation kit (Roche Molecular Systems ...
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bioRxiv - Genomics 2024Quote: ... Both FFPE extracted DNA and PBMC DNA Libraries were prepared using KAPA HyperPlus Kit (Roche) following the recommended protocol ...
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bioRxiv - Genomics 2024Quote: ... DNA was isolated following standard KAPA Pure Beads (KAPA Biosystems Catalog# KK8000) protocol with a 1.5X ratio of beads to DNA ...
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bioRxiv - Genomics 2024Quote: ... Cells were then transfected with the Tet-pLKO-puro plasmids targeting either UHRF1 or DNMT1 transcripts and the accompanying lentiviral packaging (psPAX2) and envelope (pMD.2G) plasmids with Opti-MEM and Xtreme HP Gene transfection reagent (Roche) per the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... and Kapa Illumina Library Quantification qPCR assays (Kapa Biosystems). Individually indexed libraries were pooled and 50 bp ...
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bioRxiv - Genomics 2024Quote: ... and protease inhibitor cocktail (Roche cOmplete Mini tablets, EDTA-free)) on ice and centrifuged to remove insoluble components ...
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bioRxiv - Genomics 2024Quote: ... Protease inhibitor cocktail (Roche cOmplete Mini tablets, EDTA-free)) and incubated for 10 minutes rotating at 4°C ...
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bioRxiv - Genomics 2024Quote: ... with SYBR Green 2× PCR Master Mix I (Roche, Cat# 04887352001) and 1 μM of forward and reverse primer respectively ...
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bioRxiv - Genomics 2024Quote: ... 25× protease inhibitor cocktail (Roche), 1:1000 dilution of SUPERase In™ RNase (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... and 1.0% Nonidet P-40) containing protease and phosphatase inhibitors (PMSF, NaF, Na3VO4, and Roche PhosSTOP). Lysates were cleared by centrifuging at 10,000 g for 10 min at 4° C ...
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bioRxiv - Immunology 2024Quote: ... and subsequent PCR with the KAPA HiFi HotStart Ready Mix (Roche) and ISO SMART primer as per the standard protocol ...
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bioRxiv - Immunology 2024Quote: ... Libraries were quantified by qPCR measured by Kapa Library Quantification kit (Kapa Biosystems, Woburn, MA) using ABI’s 9700HT real-time PCR system (Lifetech ...
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bioRxiv - Genomics 2024Quote: ... Oligo pool amplification PCRs were assembled with 1X KAPA HiFi HotStart Ready Mix (Roche KK2601), 1X EvaGreen qPCR dye (Biotium 31000) ...
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bioRxiv - Genetics 2024Quote: ... supplemented with 1X Protease Inhibitor Cocktail (Roche) and then centrifuged for 10 min at 13,200 rpm to remove cell debris.
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bioRxiv - Genetics 2024Quote: ... In order to link the sequencing adaptors and double indexed libraries the second PCR step were performed using TG Nextera® XT Index Kit (FC-131-200(1,2,3) and the Kappa Hifi Hot Start (Roche KK2602) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Miniaturization involved testing libraries at 1/6th (IDT mini) or 1/8th (Roche mini, Illumina mini) of reaction volume tailoring input DNA to each kit between 24-45 ng total (Table 2) ...
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bioRxiv - Genomics 2024Quote: ... the dialyzed sample was loaded again onto a cOmplete His-tag purification column (Roche), and the untagged Tn5 was collected in the flow-through ...
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bioRxiv - Genomics 2024Quote: ... cDNA was synthesized with 200ng of the stored RNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche, 04897030001) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... PCR was performed with KAPA HiFi DNA polymerase (Kapa Biosystems cat. KK2502) using barcoded indexing primers for Illumina ...
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bioRxiv - Genomics 2024Quote: ... and finally quantified by qPCR (Kapa Biosystems, Wilmington, MA, USA). These libraries were sequenced with 150 bp paired-end reads on an Illumina HiSeq 4000 or NovaSeq 6000 instrument (Illumina ...
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bioRxiv - Immunology 2024Quote: ... + 0.2 mg/mL DNAse I (Roche, cat. 10104159001) and incubated on a shaker at 37°C for 1 hour ...
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bioRxiv - Immunology 2024Quote: ... Cells were then rinsed fast with ice cold PBS and lysed using M-PER lysis buffer (supplemented with protease inhibitor (Roche) and HALT phosphatase inhibitor (Thermo ScientificTM #87786 ...