Labshake search
Citations for Roche :
1201 - 1250 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... PhosSTOP (Roche, 4906845001) and 10 mM NEM (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2024Quote: ... PhosSTOP (Roche, 49068450001) and 20 mM NEM (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2024Quote: ... and PhosSTOP (Roche, 49068450001) for 10 mins on ice ...
-
bioRxiv - Cell Biology 2024Quote: ... and EDTA-free protease inhibitor cocktail (Roche). Protein concentration was measured using the Bradford method (Bio-Rad # 500-0006) ...
-
bioRxiv - Cell Biology 2024Quote: ... EDTA-free Protease Inhibitor Cocktail Tablet was from Roche (catalog no. 11836170001). Protein A/G PLUS-Agarose (Catalog no ...
-
bioRxiv - Cell Biology 2024Quote: ... supplemented with protease inhibitor cocktail (Rowe Scientific; CP2778) and PhosSTOP EASYpack Phosphatase Inhibitor Cocktail (Roche; 4906837001), and kept on ice for 30 minutes ...
-
bioRxiv - Cell Biology 2024Quote: ... and from WT and Hcar1 KO mice were lysed in RIPA buffer (cell signaling) supplemented with 0.1 mg/ml PMSF and proteinase inhibitor cocktail (11697498001, Roche, USA), and then were homogenized thoroughly (BRANSON Sonifier 150 ...
-
bioRxiv - Cell Biology 2024Quote: ... and EDTA-free protease inhibitor cocktail (Roche) and lysates spun at 4 °C at 10,000 x g for 10 min to yield supernatants for analysis ...
-
bioRxiv - Cell Biology 2024Quote: ... Immunoprecipitation of GFP/YFP-tagged proteins was performed with anti-GFP antibodies (11814460001, Roche) using a method we previously described (Ishii and Akiyoshi ...
-
bioRxiv - Cell Biology 2024Quote: ... Cell growth was monitored using a CASY cell counter (Roche). Expression of GFP fusion proteins and RNAi were induced with doxycycline at a final concentration of 10 ng/mL and 1 µg/mL ...
-
bioRxiv - Cell Biology 2024Quote: Cell viability staining was performed using the In situ cell death detection kit (Roche Diagnostic). In summary ...
-
bioRxiv - Cell Biology 2024Quote: ... and phospho-stop (Roche). Spheroids were sonicated three times with an ultrasonic processor (UP50H ...
-
bioRxiv - Cell Biology 2024Quote: ... 10 mM NaF and 5 mM Na3VO4) containing protease/phosphatase inhibitors (Roche). Cells were incubated in lysis buffer for 2-3 hours in rotator at 4°C ...
-
bioRxiv - Cell Biology 2024Quote: ... and protease inhibitors (Roche, #05 892 970 001) for four hours at 4°C ...
-
bioRxiv - Cell Biology 2024Quote: ... ChIP-qPCR was performed on a LightCycler 480 instrument (Roche) using SYBR Green I Master Mix (4887352001 ...
-
bioRxiv - Cell Biology 2024Quote: ... using SYBR Green I Master Mix (4887352001, Roche). The relative expression of genes was normalized to the expression of Tuba1a ...
-
bioRxiv - Cell Biology 2024Quote: ... with protease and phosphatase inhibitors (Roche, Hong Kong). After quantification ...
-
bioRxiv - Cell Biology 2024Quote: ... ChIP-qPCR was performed on a LightCycler 480 instrument (Roche) using SYBR Green I Master Mix (4887352001 ...
-
bioRxiv - Cell Biology 2024Quote: ... using SYBR Green I Master Mix (4887352001, Roche). Specific primers for ChIP-qPCR analysis are listed in Suppl ...
-
bioRxiv - Cell Biology 2024Quote: ... RT-qPCR was performed on a LightCycler 480 instrument (Roche) using SYBR Green I Master Mix (4887352001 ...
-
bioRxiv - Cell Biology 2024Quote: ... a protease inhibitor cocktail (04,693,159,001) was purchased from Roche; and rhodamine phalloidin (RM02835 ...
-
bioRxiv - Cell Biology 2024Quote: ... The glucose levels were measured using a glucometer (ACCUCHEK Active, Roche Applied Science).
-
bioRxiv - Cell Biology 2024Quote: ... 1X complete protease inhibitor cocktail (Roche, Cat. # 04693132001), 1 mM sodium orthovanadate (Na3VO4 ...
-
bioRxiv - Cell Biology 2024Quote: ... For pulldown experiments mouse brain extracts were obtained by homogenizing four brains in homogenization buffer (20 mM HEPES, 320 mM sucrose, pH 7.5) containing complete EDTA-free protease inhibitor cocktail (Roche). The homogenate was centrifuged at 1000 x g for 15 min at 4°C ...
-
bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used: anti-HA (Rat, Roche #ROAHAHA, 1:500), anti-RFP (Rabbit ...
-
bioRxiv - Cell Biology 2024Quote: ... Complete EDTA-free protease inhibitor cocktail tablets were from Roche Diagnostics GmbH (Mannheim ...
-
bioRxiv - Cell Biology 2024Quote: ... anti-GFP (1:3000, Roche, cat # 1814460001) and anti-Flag (1:3000 ...
-
bioRxiv - Cell Biology 2024Quote: ... two steps of transfections were performed: DNA using XtremeGENE™ 9 (Roche) followed by siRNA (4392420-s30722 ...
-
bioRxiv - Immunology 2024Quote: ... + 0.2 mg/mL DNAse I (Roche, cat. 10104159001) and incubated on a shaker at 37°C for 1 hour ...
-
bioRxiv - Immunology 2024Quote: ... Cells were then rinsed fast with ice cold PBS and lysed using M-PER lysis buffer (supplemented with protease inhibitor (Roche) and HALT phosphatase inhibitor (Thermo ScientificTM #87786 ...
-
bioRxiv - Microbiology 2024Quote: ... Lactate was quantified using a lactate assay kit (D-Lactic/L-Lactic acid UV method, r-biopharm, Roche). All samples were measured using fresh growth medium as control.
-
bioRxiv - Immunology 2024Quote: ... Brains were homogenized using a glass Potter and digested for 45 min at room temperature (RT) in Hanks’ balanced salt solution (HBSS) medium with collagenase D (1 mg/ml, Roche Diagnostics cat # 11088882001) and deoxyribonuclease (DNase ...
-
Regulatory T cells control type 1-driven immunopathology restraining GM-CSF-producing helper T cellsbioRxiv - Immunology 2024Quote: ... then digested in 0.25 mg/ml Liberase (Roche, 5401119002) and 200U/ml DNase I (Sigma ...
-
bioRxiv - Immunology 2024Quote: ... Transcriptome libraries were generated using the KAPA RNA HyperPrep (Roche) using a poly-A selection (Thermo Scientific) ...
-
bioRxiv - Immunology 2024Quote: ... quantified using the Qubit 3.0 Fluorometer as well as by quantitative PCR (KAPA Biosystems, Wilmington, MA, USA), and sequenced on a partial S4 lane of an Illumina NovaSeq 6000 with a paired-end ...
-
bioRxiv - Immunology 2024Quote: ... and by qPCR using the KAPA Library Quantification Kit for Illumina (Roche, Basel, Switzerland). Libraries were pooled and sequenced on the Illumina NovaSeq 6000 (Illumina ...
-
bioRxiv - Microbiology 2024Quote: ... genomic DNA was obtained by automated purification using the MagNA Pure 96 DNA and Viral NA Small Volume Kit and DNA Blood ds SV 2.0 protocol (Roche Diagnostics). The DNA was quantified with Qubit fluorometer (Invitrogen ...
-
bioRxiv - Microbiology 2024Quote: Tissues were weighed and homogenized with zirconia beads in a MagNA Lyser instrument (Roche Life Science) in 1 ml of DMEM medium supplemented with 2% heat inactivated FBS ...
-
bioRxiv - Microbiology 2024Quote: In-situ detection of DNA fragments following treatment of cells with MLT 5 μM and 10 μM for 24 h was measured by TUNEL using a Cell Death Detection kit (In-Situ Cell Death Detection Kit, POD; Roche; Cat. No. 11 684 817 910). 1000 μL of cells were centrifuged (3000 rpm for 5 min) ...
-
bioRxiv - Microbiology 2024Quote: ... Primer pair mix with 7 µL of 2X SYBR green (LightCycler 480 SYBR Green I Master; Roche; Cat No. 04707516001) and 1 µL of 10 µM of forward and reverse primer were prepared for all the gene targets ...
-
bioRxiv - Microbiology 2024Quote: ... cDNAs were diluted to 1 ng/ µL and used for qPCR analysis in a 10 µL reaction mix containing 5 µL of LightCycler® 480 SYBR Green I Master mix (Roche), 300 nM of each primer ...
-
bioRxiv - Microbiology 2024Quote: ... qPCR was performed in triplicate using a LightCycler ® 480 System (Roche) with preheating at 95 °C for 5 minutes and then 40 cycles of 95 °C for 15 s and 60 °C for 60 s ...
-
bioRxiv - Microbiology 2024Quote: ... 0.1% SDS) supplemented with cOmplete Mini Protease Inhibitor Cocktail (Roche, Penzberg, Germany). Cell-free virions were further cleared of debris by centrifugation at 1000 × g for 5 min at 4°C and resuspended in RIPA buffer supplemented with cOmplete Mini Protease Inhibitor Cocktail ...
-
The transcriptomic landscape of monosomy X (45,X) during early human fetal and placental developmentbioRxiv - Genetics 2024Quote: ... RNA was used to generate cDNA libraries using a KAPA mRNA HyperPrep Kit (Roche, Basel, Switzerland) on a Hamilton Starlet robotic platform (Hamilton Company ...
-
Genomic dissection and mutation-specific target discovery for breast cancer PIK3CA hotspot mutationsbioRxiv - Genomics 2024Quote: ... 10 µg/ml insulin (Roche), 0.5 µg/mL hydrocortisone (Sigma) ...
-
bioRxiv - Genomics 2024Quote: ... The probe was labeled with alpha-32P CTP using the High Prime DNA labeling kit (Roche, #11585584001). The labelled probe was purified on a G50 sephadex column (Cytiva ...
-
bioRxiv - Genomics 2024Quote: ... Libraries were quantified by qPCR (KAPA Biosystems) and sequenced with 151 bp paired-end reads on an Illumina NextSeq instrument ...
-
bioRxiv - Genomics 2024Quote: ... treated with USER enzyme and amplified by PCR using HiFi HotStart Uracil+ Ready Mix (Kapa/Roche). Completed libraries were quantified by qPCR using Kapa Library Quantification kit (Kapa Biosystems ...
-
bioRxiv - Genomics 2024Quote: ... using Kapa HiFi Polymerase (KAPA Biosystems). Libraries were quantified by qPCR (KAPA Biosystems ...
-
bioRxiv - Genomics 2024Quote: ... Completed libraries were quantified by qPCR using Kapa Library Quantification kit (Kapa Biosystems) and sequenced with 151-bp paired-end reads on an Illumina MiSeq or MiniSeq instrument.